##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364382_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268427 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.099155053259373 31.0 31.0 34.0 28.0 34.0 2 31.148420910172554 33.0 31.0 34.0 27.0 34.0 3 31.29455785881582 33.0 31.0 34.0 28.0 34.0 4 34.7149760605036 37.0 35.0 37.0 32.0 37.0 5 34.794218196133265 37.0 35.0 37.0 32.0 37.0 6 34.85844155443397 37.0 35.0 37.0 32.0 37.0 7 34.77809028018093 37.0 35.0 37.0 32.0 37.0 8 34.7130390354197 37.0 35.0 37.0 32.0 37.0 9 36.2512772066282 39.0 37.0 39.0 32.0 39.0 10-11 36.15675267487118 39.0 37.0 39.0 31.5 39.0 12-13 36.17188915490779 39.0 37.0 39.0 32.0 39.0 14-15 37.476469156821004 40.0 38.0 41.0 32.0 41.0 16-17 37.47885296727644 40.0 38.0 41.0 31.5 41.0 18-19 37.490047067858036 40.0 38.0 41.0 31.0 41.0 20-21 37.52251361846778 40.0 38.0 41.0 31.0 41.0 22-23 37.48732976639759 40.0 38.0 41.0 31.0 41.0 24-25 37.49858514292062 40.0 38.0 41.0 31.0 41.0 26-27 37.45760652646085 40.0 38.0 41.0 31.0 41.0 28-29 37.459232763496466 40.0 38.0 41.0 31.0 41.0 30-31 37.352870292938675 40.0 37.5 41.0 30.5 41.0 32-33 37.311392652265205 40.0 37.0 41.0 30.0 41.0 34-35 37.30544315510264 40.0 37.0 41.0 30.0 41.0 36-37 37.243023469567234 40.0 37.0 41.0 30.0 41.0 38-39 37.149017799558905 40.0 37.0 41.0 30.0 41.0 40-41 37.08607153767787 40.0 37.0 41.0 30.0 41.0 42-43 36.95446646508792 40.0 37.0 41.0 30.0 41.0 44-45 36.83860710527604 40.0 37.0 41.0 30.0 41.0 46-47 36.72670996245416 40.0 36.0 41.0 30.0 41.0 48-49 36.57643821026641 40.0 36.0 41.0 30.0 41.0 50-51 36.4527941608877 40.0 36.0 41.0 30.0 41.0 52-53 36.29056257926749 40.0 35.0 41.0 29.0 41.0 54-55 36.101038737415834 39.0 35.0 41.0 29.0 41.0 56-57 35.91250280480703 39.0 35.0 41.0 29.0 41.0 58-59 35.68619356055981 39.0 35.0 41.0 28.5 41.0 60-61 35.44107370437753 38.5 35.0 40.5 28.0 41.0 62-63 35.17346756144235 38.0 34.0 40.0 28.0 41.0 64-65 34.83180283077216 37.0 34.0 40.0 28.0 41.0 66-67 34.52025919282393 37.0 34.0 40.0 27.5 41.0 68-69 34.19513125174405 36.0 34.0 39.0 27.0 41.0 70-71 33.82791335141047 36.0 33.5 39.0 26.0 41.0 72-73 33.47526347552731 35.0 33.0 38.5 26.0 40.0 74-75 33.12504237517892 35.0 33.0 37.5 26.0 40.0 76-77 32.89398997631398 35.0 33.0 37.0 26.0 39.0 78-79 32.72156895505123 35.0 33.0 37.0 26.0 39.0 80-81 32.458220167543416 35.0 33.0 36.0 26.0 39.0 82-83 32.16055575074711 35.0 33.0 36.0 25.5 37.0 84-85 31.901847403509127 35.0 33.0 35.5 25.0 37.0 86-87 31.69905577741757 35.0 33.0 35.0 25.0 36.5 88-89 31.429325034484247 35.0 32.5 35.0 24.0 36.0 90-91 31.304997471816375 35.0 32.0 35.0 24.0 36.0 92-93 31.154266811319033 35.0 32.0 35.0 23.0 36.0 94-95 31.040980379796217 35.0 32.0 35.0 23.0 35.5 96-97 30.933250221408933 35.0 32.0 35.0 22.0 35.0 98-99 30.794597974719927 35.0 32.0 35.0 20.0 35.0 100-101 30.69963503343947 35.0 32.0 35.0 19.5 35.0 102-103 29.67786642461935 33.5 30.0 34.5 18.0 35.0 104-105 30.374586222082527 34.5 31.5 35.0 18.0 35.0 106-107 30.694756763166723 35.0 32.0 35.0 19.0 35.0 108-109 30.729514108234472 35.0 32.0 35.0 19.0 35.0 110-111 30.64754166653809 35.0 32.0 35.0 18.0 35.0 112-113 30.54775666133404 35.0 32.0 35.0 16.0 35.0 114-115 30.435951212007264 35.0 32.0 35.0 8.5 35.0 116-117 30.302614102195044 35.0 32.0 35.0 3.5 35.0 118-119 30.114486161556 35.0 32.0 35.0 2.0 35.0 120-121 29.924203864616317 35.0 32.0 35.0 2.0 35.0 122-123 29.740360611119513 35.0 32.0 35.0 2.0 35.0 124-125 28.938173015926896 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2909392904067083 1101 2 0.2489611105280538 1101 3 0.3166013796338589 1101 4 0.4061709002453071 1101 5 0.25833139732605304 1101 6 0.26409027511066085 1101 7 0.264729074519515 1101 8 0.2249491549923377 1101 9 0.4174852972970555 1101 10-11 0.37743714737624146 1101 12-13 0.4646273249919446 1101 14-15 0.5342207513889932 1101 16-17 0.4784017020277247 1101 18-19 0.3975094365447873 1101 20-21 0.46845956356347784 1101 22-23 0.5295396690290985 1101 24-25 0.5199769108568688 1101 26-27 0.5290751092426973 1101 28-29 0.5194079430847154 1101 30-31 0.5669864397356434 1101 32-33 0.4543167205326881 1101 34-35 0.4621739633264639 1101 36-37 0.41843440584909786 1101 38-39 0.5120721112990196 1101 40-41 0.3808552600751085 1101 42-43 0.4486098404880039 1101 44-45 0.4353771876356092 1101 46-47 0.4224599616983724 1101 48-49 0.307328638889274 1101 50-51 0.18482023193585206 1101 52-53 0.2383519322593557 1101 54-55 0.3149783597536313 1101 56-57 0.18324892496030287 1101 58-59 0.3333540044790695 1101 60-61 0.27754994686371504 1101 62-63 0.22776431767916705 1101 64-65 0.3559780513098474 1101 66-67 0.17594882604002748 1101 68-69 0.1698599578115818 1101 70-71 0.18809340895461446 1101 72-73 0.20615372925075803 1101 74-75 0.11709995558961594 1101 76-77 0.21278593697167025 1101 78-79 0.2779581736854553 1101 80-81 0.23484528312273056 1101 82-83 0.1372727349428402 1101 84-85 0.22845369063302456 1101 86-87 0.17210873247080372 1101 88-89 0.2344316318710007 1101 90-91 0.09266856175564442 1101 92-93 0.05443808640395886 1101 94-95 0.06048600749393529 1101 96-97 -0.008033733241145313 1101 98-99 -0.053741556513031696 1101 100-101 -0.037223015827475336 1101 102-103 -0.1465712300970452 1101 104-105 -0.09267887552119092 1101 106-107 -0.10590069813947522 1101 108-109 -0.06284052612688384 1101 110-111 -0.14857690781647293 1101 112-113 -0.12515278855730116 1101 114-115 -0.22791361195464788 1101 116-117 -0.25790171993560307 1101 118-119 -0.17966203718897233 1101 120-121 -0.30693107447415 1101 122-123 -0.3542348655352967 1101 124-125 -0.4704717684133861 1102 1 0.13323663077759917 1102 2 0.15336396602605262 1102 3 0.27960029251972074 1102 4 0.2712460348901544 1102 5 0.2498048593161215 1102 6 0.24752747190330382 1102 7 0.248003199386865 1102 8 0.1766374875468273 1102 9 0.3303024527326599 1102 10-11 0.38020754431559567 1102 12-13 0.26722188869094055 1102 14-15 0.3332881279614739 1102 16-17 0.3626927057522451 1102 18-19 0.4230378803917816 1102 20-21 0.49789495897349667 1102 22-23 0.40692870396522096 1102 24-25 0.42576568553134564 1102 26-27 0.3681469023064352 1102 28-29 0.4262831111895764 1102 30-31 0.4663666414970322 1102 32-33 0.5175045931320952 1102 34-35 0.42540513599041674 1102 36-37 0.3279497151509929 1102 38-39 0.36888511123531487 1102 40-41 0.3570069279203736 1102 42-43 0.3352561620821817 1102 44-45 0.27299617993183034 1102 46-47 0.292595851445995 1102 48-49 0.3283249420477645 1102 50-51 0.33255936028998434 1102 52-53 0.349759221833871 1102 54-55 0.39988007120165747 1102 56-57 0.32332477790959757 1102 58-59 0.22904750267552032 1102 60-61 0.21670004712363777 1102 62-63 0.26727639533176273 1102 64-65 0.2822501300425486 1102 66-67 0.39663607090381703 1102 68-69 0.24555324652259003 1102 70-71 0.27580385894376036 1102 72-73 0.32988577776566785 1102 74-75 0.21697132383993534 1102 76-77 0.27218500587613903 1102 78-79 0.34078694210995764 1102 80-81 0.23272467543429087 1102 82-83 0.26559405217193444 1102 84-85 0.2636101527160051 1102 86-87 0.24391601816311237 1102 88-89 0.26962638750863377 1102 90-91 0.20356688818796798 1102 92-93 0.25563847643125825 1102 94-95 0.12606340751244005 1102 96-97 0.09600846135526098 1102 98-99 0.06558759806618042 1102 100-101 0.04199164630854213 1102 102-103 -0.12720502157888802 1102 104-105 0.03293897440670435 1102 106-107 0.02138582826611568 1102 108-109 0.12682519180134832 1102 110-111 0.09468585294605703 1102 112-113 0.028325856563881757 1102 114-115 0.020490719166495808 1102 116-117 -0.11954388785624914 1102 118-119 -0.07611972482907348 1102 120-121 -0.16710290442847153 1102 122-123 -0.09345061737999316 1102 124-125 -0.0774071203407658 1103 1 0.27225993448461594 1103 2 0.2964619027546611 1103 3 0.3373747229044852 1103 4 0.36889461714022787 1103 5 0.21743298912072362 1103 6 0.2304832325181465 1103 7 0.23690932860819203 1103 8 0.1859587815910828 1103 9 0.3844899186963744 1103 10-11 0.49569567066583886 1103 12-13 0.3073571938401045 1103 14-15 0.34215691046996 1103 16-17 0.30089526896181695 1103 18-19 0.3526346548136772 1103 20-21 0.4030689935227443 1103 22-23 0.3969184258327232 1103 24-25 0.4878146015139819 1103 26-27 0.5078922707760682 1103 28-29 0.3813201039722287 1103 30-31 0.5168230226694561 1103 32-33 0.5150109898302588 1103 34-35 0.4654798482923752 1103 36-37 0.35208001431166025 1103 38-39 0.3493872390635602 1103 40-41 0.43336566589028536 1103 42-43 0.4112827247783102 1103 44-45 0.39264072077112644 1103 46-47 0.3437923242249852 1103 48-49 0.36235981219314084 1103 50-51 0.41357029814833624 1103 52-53 0.32641979146327316 1103 54-55 0.27204813424086893 1103 56-57 0.21332695811951652 1103 58-59 0.3273402378574062 1103 60-61 0.18047170647102462 1103 62-63 0.2450591124018615 1103 64-65 0.30724803005679746 1103 66-67 0.16030065457338338 1103 68-69 0.139443892478198 1103 70-71 0.26422991726579426 1103 72-73 0.14473734888534295 1103 74-75 0.13460384237880163 1103 76-77 0.1531693281847879 1103 78-79 0.1763250717163629 1103 80-81 0.21861262963288652 1103 82-83 0.11939276122339493 1103 84-85 0.05698170107089595 1103 86-87 0.16497609096891352 1103 88-89 0.22461685026998524 1103 90-91 0.18211178210801648 1103 92-93 0.1700845851340702 1103 94-95 0.0862691966776481 1103 96-97 0.06200067866650372 1103 98-99 0.06801442654028733 1103 100-101 0.06991352872632817 1103 102-103 -0.14162248553227386 1103 104-105 -0.012618824279694962 1103 106-107 0.015064823972707586 1103 108-109 0.02207139035523653 1103 110-111 0.015928459344547008 1103 112-113 -0.0461426582790061 1103 114-115 -0.11006402628219547 1103 116-117 -0.08375744083393855 1103 118-119 -0.1555405723550045 1103 120-121 -0.30027130629684606 1103 122-123 -0.3228448493562155 1103 124-125 -0.3252258250318043 1104 1 0.22452869776123663 1104 2 0.2712966851842573 1104 3 0.31695730453961346 1104 4 0.3703514527334164 1104 5 0.33883314659115626 1104 6 0.3686305862752022 1104 7 0.2878654325536587 1104 8 0.3099228423810345 1104 9 0.42732889908933913 1104 10-11 0.4443279622164553 1104 12-13 0.29291691136698006 1104 14-15 0.3628885675514013 1104 16-17 0.4145081294843891 1104 18-19 0.3845512535271354 1104 20-21 0.42510254352615817 1104 22-23 0.4448513220810497 1104 24-25 0.34610220881529585 1104 26-27 0.4268616071065239 1104 28-29 0.41728075321478286 1104 30-31 0.3822946803313414 1104 32-33 0.276618208754428 1104 34-35 0.33569284531429133 1104 36-37 0.3616423644596267 1104 38-39 0.1514821014267227 1104 40-41 0.20734348895980048 1104 42-43 0.21043021241332127 1104 44-45 0.06484878022417462 1104 46-47 0.16300114294393353 1104 48-49 0.1419119670197233 1104 50-51 0.0017704073103601559 1104 52-53 0.017563296463094957 1104 54-55 -0.04442362921269449 1104 56-57 -0.11305608076032314 1104 58-59 -0.06538316747355566 1104 60-61 -0.276641145157555 1104 62-63 -0.215350179664604 1104 64-65 0.08693513948190201 1104 66-67 -9.952278836493633E-4 1104 68-69 -0.01188121843921408 1104 70-71 -0.03917484261051385 1104 72-73 -0.07689807803087234 1104 74-75 -0.14046733294795644 1104 76-77 -0.09487273009050767 1104 78-79 0.04996782540806777 1104 80-81 -0.029058736493361437 1104 82-83 -0.01277277559445622 1104 84-85 -0.027326538415238133 1104 86-87 -0.009945665322646846 1104 88-89 0.02010750362250846 1104 90-91 0.02900356658884462 1104 92-93 -0.051300873847313966 1104 94-95 -0.0726660335409477 1104 96-97 -0.07526064642568642 1104 98-99 -0.07970782257195097 1104 100-101 -0.0444025187469812 1104 102-103 -0.35290039276702245 1104 104-105 -0.14931683211950997 1104 106-107 -0.028710365913983793 1104 108-109 -0.06722305408400686 1104 110-111 -0.20472553915938718 1104 112-113 -0.17855320112135686 1104 114-115 -0.29668699693109346 1104 116-117 -0.32286483061633575 1104 118-119 -0.2658874192482408 1104 120-121 -0.35848261252441915 1104 122-123 -0.4530232815236275 1104 124-125 -0.5959455922526722 1105 1 0.22011027811964112 1105 2 0.12659675038540996 1105 3 0.13625649115498106 1105 4 0.22508112995261342 1105 5 0.18563343610169625 1105 6 0.2003939315731813 1105 7 0.32048377362702496 1105 8 0.30630005268997706 1105 9 0.3987819664701746 1105 10-11 0.35553635251531546 1105 12-13 0.40412840140744066 1105 14-15 0.5078470509979311 1105 16-17 0.4435293048051534 1105 18-19 0.4409342654154855 1105 20-21 0.4832718148195383 1105 22-23 0.48124326548847307 1105 24-25 0.4471921099370917 1105 26-27 0.5153131334095207 1105 28-29 0.4012310195039106 1105 30-31 0.37708094160154815 1105 32-33 0.4412647143109112 1105 34-35 0.31375258115413374 1105 36-37 0.26497310342470826 1105 38-39 0.30001878802322324 1105 40-41 0.36444119112756823 1105 42-43 0.28335412050714126 1105 44-45 0.3006854257957201 1105 46-47 0.295084862967812 1105 48-49 0.28663628230767557 1105 50-51 0.3128896955812692 1105 52-53 0.31299620468774236 1105 54-55 0.3091752640463241 1105 56-57 0.23842170994636547 1105 58-59 0.19615513214320401 1105 60-61 0.1867546812757439 1105 62-63 0.27008130125781804 1105 64-65 0.211499588039203 1105 66-67 0.13522122929289537 1105 68-69 0.10968212069481353 1105 70-71 0.20930204295386545 1105 72-73 0.034747731105156276 1105 74-75 0.08004748528239958 1105 76-77 0.13895694381578494 1105 78-79 0.19994531453388475 1105 80-81 0.18830793442767657 1105 82-83 0.19892905093495017 1105 84-85 0.201474448670389 1105 86-87 0.16730208920765932 1105 88-89 0.19692688985057671 1105 90-91 0.20053580616636424 1105 92-93 0.20610846337375222 1105 94-95 0.22159030514197298 1105 96-97 0.15156330059697254 1105 98-99 0.04556779019766921 1105 100-101 -0.034762366772604025 1105 102-103 -0.11206801178266801 1105 104-105 -0.0021512260707368114 1105 106-107 5.268587319271489E-4 1105 108-109 0.12928586415679888 1105 110-111 0.08773254971402267 1105 112-113 0.017198234418717817 1105 114-115 0.002044205112284203 1105 116-117 -0.042362906955776936 1105 118-119 0.013639950981609417 1105 120-121 -0.08054192807838234 1105 122-123 -0.1091064622457587 1105 124-125 -0.18441608115830732 1106 1 0.3587240554475635 1106 2 0.3953107515604444 1106 3 0.3624260720953316 1106 4 0.45659851881825375 1106 5 0.39895399216596417 1106 6 0.35970154595859327 1106 7 0.3928992644249334 1106 8 0.46720417264087644 1106 9 0.5676920430513377 1106 10-11 0.6363053299068469 1106 12-13 0.4732724313198915 1106 14-15 0.6042759218463729 1106 16-17 0.5807940075830373 1106 18-19 0.5542095067464388 1106 20-21 0.46337325092351733 1106 22-23 0.5491591608209916 1106 24-25 0.545828431161155 1106 26-27 0.661197635615622 1106 28-29 0.5649167587671258 1106 30-31 0.7104887654665859 1106 32-33 0.5891857672453895 1106 34-35 0.6029955197986681 1106 36-37 0.6093303823823106 1106 38-39 0.49161533358909537 1106 40-41 0.556207085304969 1106 42-43 0.44231381308859596 1106 44-45 0.47421636434621917 1106 46-47 0.49301367516260797 1106 48-49 0.4565488994037281 1106 50-51 0.38415166478112184 1106 52-53 0.3893070400845673 1106 54-55 0.4889726726574608 1106 56-57 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2216 124-125 -0.857808098256065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33138.0 3 6463.0 4 4126.0 5 3422.0 6 3805.0 7 4508.0 8 5006.0 9 5632.0 10 6271.0 11 7128.0 12 7933.0 13 8258.0 14 8334.0 15 7931.0 16 7571.0 17 6532.0 18 6325.0 19 6127.0 20 6299.0 21 6583.0 22 7927.0 23 10406.0 24 14666.0 25 20305.0 26 24756.0 27 26271.0 28 30073.0 29 36922.0 30 44206.0 31 53316.0 32 68945.0 33 90962.0 34 126436.0 35 190281.0 36 369855.0 37 693957.0 38 306922.0 39 829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82043680632673 18.698383828939722 12.907361459396382 24.573817905337165 2 15.34166033639846 20.199206155710048 39.660383131492196 24.79875037639929 3 17.82903736476505 25.42093056370508 30.0167477308566 26.73328434067327 4 12.035342985849228 16.574893994848974 38.39670129780812 32.993061721493675 5 12.67558621334334 39.0662651797718 35.31420876466057 12.943939842224292 6 31.89480345099008 37.65071623698327 17.516662955213416 12.937817356813236 7 28.070637942192494 32.87089857147644 22.080925569002396 16.97753791732867 8 26.376130409367555 35.19321326770192 20.573075780298762 17.857580542631762 9 26.683688741140887 15.828545507525698 20.015279310288584 37.47248644104483 10-11 24.226026527668548 26.847494119265182 28.659148377904657 20.26733097516161 12-13 24.958555598978222 24.393399666416933 28.281381528526378 22.366663206078467 14-15 22.10652487625189 26.08140869949703 25.14608746137452 26.665978962876558 16-17 22.30590980916923 29.17224230836967 26.444907663846717 22.076940218614382 18-19 22.508906504134032 28.103445273629102 27.418477080919306 21.969171141317563 20-21 22.88641487833086 27.5868096233226 27.322926266290754 22.203849232055788 22-23 22.84437630128719 27.406965267121226 27.298674367744695 22.449984063846887 24-25 22.595305028550623 27.726327538862833 27.173830147498684 22.50453728508786 26-27 22.085397080310955 27.96526579106707 27.056309737168032 22.893027391453945 28-29 22.53182335462054 27.951938364161315 27.03030588905364 22.485932392164504 30-31 22.373803298591135 27.597851018086132 26.944159003854217 23.084186679468512 32-33 21.9290745625433 28.01596438409125 26.954765300762713 23.100195752602744 34-35 23.033726068298545 27.84141392522555 26.63360677761523 22.491253228860675 36-37 23.16082234131119 27.208034824154463 26.646367711877687 22.98477512265666 38-39 22.465536087066635 27.688921402903823 26.75358018465247 23.091962325377075 40-41 22.733151884973378 27.370951686949564 27.21092798757193 22.68496844050513 42-43 22.473489696860423 27.310334703529186 26.819748894655532 23.396426704954855 44-45 22.352990187553356 27.58920461145537 27.230536840042596 22.82726836094868 46-47 23.082564059271924 27.797748791948955 26.408261409395458 22.711425739383664 48-49 22.52563119730104 27.823024501119058 26.492587154005836 23.158757147574068 50-51 22.584482551124633 27.68125225100918 26.905406257287538 22.828858940578648 52-53 22.954688576956066 27.295388404565944 26.957441176088093 22.792481842389893 54-55 22.285141057096673 27.566017569123346 27.067815017449874 23.081026356330106 56-57 22.442565902524482 28.001971411013628 26.742662092569912 22.81280059389198 58-59 22.337270737497327 27.288701582382412 26.96810327916188 23.405924400958376 60-61 21.9802147803495 27.64387221801345 27.254061819939558 23.12185118169749 62-63 22.194562196704663 27.541090346744163 27.687646357634577 22.5767010989166 64-65 22.132922823542067 27.54365523878295 27.08121454823424 23.242207389440747 66-67 22.023367367946335 27.844533778997054 26.979421850953344 23.152677002103268 68-69 22.574593473175796 27.138856143772465 27.51811659691002 22.768433786141717 70-71 22.461474302131226 27.199485449692588 27.116563146039578 23.222477102136605 72-73 22.21868323295707 27.459247926329855 26.882955975438875 23.439112865274208 74-75 22.28865948477091 27.79283006574269 26.90360868440113 23.014901765085266 76-77 22.479429378792588 27.370861905171257 27.29616184058843 22.853546875447726 78-79 22.54473068132499 27.286261151398033 27.21832725227216 22.950680915004817 80-81 22.79300971695543 27.598432555724152 26.806621036974864 22.801936690345556 82-83 22.759221290246643 27.434768444454043 27.027855083209936 22.778155182089378 84-85 22.471642143713307 27.717057562998722 27.005923508840297 22.80537678444767 86-87 22.72298853057624 27.632573745392637 27.003842832260393 22.640594891770732 88-89 22.508933489780077 27.70972236580808 26.87069844008076 22.91064570433108 90-91 22.435971332437703 27.633735853298607 27.01145181607883 22.91884099818486 92-93 22.722152986043483 27.24315300235452 27.222455621862608 22.812238389739388 94-95 22.284955918404414 26.956802725436834 27.667257242637678 23.090984113521074 96-97 22.690332751137134 26.688552659073135 27.544808681982452 23.076305907807278 98-99 22.347679652267445 27.223826380592577 27.113242315101765 23.315251652038214 100-101 22.384164203482182 26.800615410387472 27.575036203852488 23.240184182277858 102-103 22.251258815677293 26.534163697616208 27.717066904367037 23.497510582339462 104-105 21.994779605635838 27.207415234717164 27.233711174914955 23.564093984732043 106-107 22.366820027827202 26.926742859359763 27.06689285821352 23.639544254599514 108-109 22.438303130547716 26.75165062077095 27.078600716191094 23.731445532490238 110-111 22.320509892884758 27.040933733850935 27.39871927236755 23.239837100896757 112-113 22.263164286959665 27.221038436435848 27.63152935253616 22.88426792406833 114-115 22.16838772476472 27.60539276626585 27.11922912114944 23.106990387819987 116-117 22.166115431963384 27.50187632872177 27.422877654550526 22.90913058476432 118-119 22.079581120890612 27.50563479639205 27.072205883033956 23.34257819968338 120-121 21.973110975368076 27.800330177418008 27.14844029857491 23.078118548639004 122-123 22.02246532211544 27.815048049444073 27.26424890795716 22.898237720483323 124-125 22.0652841648131 27.85332498962363 27.18172101576657 22.8996698297967 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 629.0 1 543.5 2 406.5 3 316.5 4 263.5 5 232.0 6 202.0 7 191.0 8 184.0 9 166.0 10 172.5 11 180.0 12 168.0 13 177.0 14 210.0 15 226.5 16 273.0 17 342.5 18 411.0 19 548.5 20 776.5 21 1164.0 22 1734.5 23 2604.5 24 3822.5 25 5472.0 26 8267.0 27 12196.0 28 15741.5 29 19568.5 30 25030.0 31 32284.5 32 43233.0 33 49696.0 34 50447.0 35 54514.5 36 62673.0 37 69558.0 38 72913.0 39 76432.0 40 76609.5 41 76330.0 42 76680.0 43 77517.0 44 79531.0 45 82397.5 46 82294.5 47 80029.5 48 78287.5 49 74913.5 50 74243.0 51 73084.0 52 72672.5 53 76186.0 54 79487.5 55 75791.0 56 68447.5 57 63401.5 58 60172.5 59 52849.0 60 40164.5 61 31860.5 62 27820.0 63 21159.0 64 14516.5 65 10802.0 66 7853.5 67 5909.5 68 4355.0 69 3003.5 70 2062.0 71 1283.0 72 873.0 73 649.5 74 475.0 75 359.0 76 231.0 77 138.0 78 91.0 79 53.0 80 34.0 81 23.0 82 20.0 83 18.5 84 17.0 85 14.5 86 9.0 87 8.0 88 7.0 89 7.0 90 6.5 91 4.0 92 3.0 93 3.0 94 2.5 95 1.5 96 0.5 97 0.5 98 1.5 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042716825359599406 2 0.009874684087255177 3 0.0026450046662290653 4 0.006215760965638303 5 0.023099707418400502 6 0.029624052261765534 7 0.003129922188371061 8 4.4083411103817754E-5 9 0.0 10-11 1.3225023331145326E-4 12-13 0.0011682103942511706 14-15 3.747089943824509E-4 16-17 1.5429193886336214E-4 18-19 2.2041705551908877E-5 20-21 2.2041705551908877E-5 22-23 0.0 24-25 0.0 26-27 2.2041705551908877E-5 28-29 2.865421721748154E-4 30-31 1.7633364441527102E-4 32-33 1.1020852775954439E-4 34-35 8.155431054206285E-4 36-37 6.612511665572663E-5 38-39 1.5429193886336214E-4 40-41 5.730843443496308E-4 42-43 7.273762832129929E-4 44-45 0.0014327108608740771 46-47 2.2041705551908877E-5 48-49 0.0 50-51 0.0 52-53 0.0011241269831473528 54-55 0.0031960473050267873 56-57 4.4083411103817754E-5 58-59 5.29000933245813E-4 60-61 7.273762832129929E-4 62-63 4.849175221419953E-4 64-65 0.0012343355109068971 66-67 0.0022041705551908877 68-69 0.0028654217217481542 70-71 0.0016972113274969834 72-73 0.002005795205223708 74-75 0.0016751696219450747 76-77 9.698350442839906E-4 78-79 0.002005795205223708 80-81 9.25751633180173E-4 82-83 3.5266728883054203E-4 84-85 3.5266728883054203E-4 86-87 0.002116003732983252 88-89 0.0060173856156711236 90-91 4.1879240548626866E-4 92-93 0.001058001866491626 94-95 8.816682220763551E-4 96-97 2.2041705551908877E-4 98-99 7.494179887649018E-4 100-101 9.698350442839906E-4 102-103 0.0011020852775954439 104-105 7.053345776610841E-4 106-107 0.0063039277878459395 108-109 0.0016751696219450747 110-111 0.0012784189220107149 112-113 0.002005795205223708 114-115 0.0024245876107099767 116-117 0.0015208776830817124 118-119 0.045956956075730006 120-121 0.011131061303713982 122-123 0.003813215060480236 124-125 0.0022702956718466147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2268427.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.865602327055875 #Duplication Level Percentage of deduplicated Percentage of total 1 81.74586844749076 44.03290441672361 2 9.95810060120089 10.72798173834206 3 3.3355096325504827 5.390077062750856 4 1.5410340170905759 3.320349021482655 5 0.825167855175833 2.2224081769985524 6 0.5201835442412839 1.681199995870768 7 0.3222887294042983 1.2152193572808834 8 0.23764281525384368 1.0240618705876439 9 0.17753420218316487 0.8606688060824543 >10 1.0875016389833494 11.576031460268123 >50 0.13488399527034214 5.077679939043314 >100 0.10808303551370724 10.38503654287675 >500 0.005315559121317489 1.9261374752061793 >1k 8.859265202195815E-4 0.5602441364861083 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 2 8.816682220763551E-5 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 3 8.816682220763551E-5 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 4 8.816682220763551E-5 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 5 1.7633364441527102E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 6 2.2041705551908877E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 7 2.2041705551908877E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 8 2.2041705551908877E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 9 2.2041705551908877E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 10-11 2.4245876107099765E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 12-13 2.645004666229065E-4 0.0 0.0 4.4083411103817754E-5 4.4083411103817754E-5 14-15 2.645004666229065E-4 0.0 0.0 6.612511665572663E-5 4.4083411103817754E-5 16-17 2.645004666229065E-4 0.0 0.0 8.816682220763551E-5 4.4083411103817754E-5 18-19 3.5266728883054203E-4 0.0 0.0 1.3225023331145326E-4 4.4083411103817754E-5 20-21 3.967506999343598E-4 0.0 0.0 1.3225023331145326E-4 6.612511665572663E-5 22-23 4.4083411103817754E-4 0.0 0.0 1.7633364441527102E-4 8.816682220763551E-5 24-25 4.849175221419953E-4 0.0 0.0 5.069592276939042E-4 8.816682220763551E-5 26-27 5.069592276939042E-4 0.0 0.0 9.257516331801728E-4 1.3225023331145326E-4 28-29 6.392094610053574E-4 0.0 0.0 0.0033282975383382403 1.3225023331145326E-4 30-31 6.612511665572663E-4 0.0 0.0 0.012872356042314785 1.5429193886336214E-4 32-33 7.053345776610841E-4 0.0 0.0 0.03381197631662822 1.7633364441527102E-4 34-35 8.375848109725373E-4 0.0 0.0 0.06453811385598919 1.7633364441527102E-4 36-37 8.816682220763551E-4 0.0 0.0 0.11549853709200253 1.7633364441527102E-4 38-39 9.698350442839906E-4 0.0 0.0 0.1946503017289073 1.7633364441527102E-4 40-41 9.918767498358996E-4 0.0 0.0 0.2793345344593412 1.7633364441527102E-4 42-43 0.0011241269831473526 0.0 0.0 0.36456980982857284 1.7633364441527102E-4 44-45 0.0012343355109068971 0.0 0.0 0.47059041353325454 1.7633364441527102E-4 46-47 0.0013445440386664416 0.0 0.0 0.6018708118004238 1.7633364441527102E-4 48-49 0.0014547525664259859 0.0 0.0 0.7400061804942367 1.983753499671799E-4 50-51 0.0014547525664259859 0.0 0.0 0.8964141230905821 2.2041705551908877E-4 52-53 0.0014547525664259859 0.0 0.0 1.042308172138667 2.645004666229065E-4 54-55 0.0014988359775298036 0.0 0.0 1.1994875744293292 3.085838777267243E-4 56-57 0.0014988359775298036 0.0 0.0 1.3793038083218019 3.3062558327863316E-4 58-59 0.0014988359775298036 0.0 0.0 1.5805445800107298 3.5266728883054203E-4 60-61 0.0014988359775298036 0.0 0.0 1.803033555851698 3.5266728883054203E-4 62-63 0.0015208776830817126 0.0 0.0 2.020298647476864 3.967506999343598E-4 64-65 0.0015870027997374392 0.0 0.0 2.239679742835013 4.1879240548626866E-4 66-67 0.0017633364441527102 0.0 0.0 2.498735908186598 4.628758165900864E-4 68-69 0.0017633364441527102 0.0 0.0 2.7908766735715984 4.849175221419953E-4 70-71 0.0018735449719122547 0.0 0.0 3.104662393808573 4.849175221419953E-4 72-73 0.001983753499671799 0.0 0.0 3.4264933365719945 5.29000933245813E-4 74-75 0.001983753499671799 0.0 0.0 3.7463405258357443 5.951260499015397E-4 76-77 0.001983753499671799 0.0 0.0 4.092615720056233 6.171677554534486E-4 78-79 0.001983753499671799 0.0 0.0 4.486016080746703 6.171677554534486E-4 80-81 0.002138045438535161 0.0 0.0 4.906020780038326 6.171677554534486E-4 82-83 0.0022041705551908877 0.0 0.0 5.35016114690929 6.612511665572663E-4 84-85 0.002314379082950432 0.0 0.0 5.797343269146417 6.612511665572663E-4 86-87 0.0024245876107099767 0.0 0.0 6.2515126120435 7.053345776610841E-4 88-89 0.0024245876107099767 0.0 0.0 6.728869829181191 7.494179887649018E-4 90-91 0.0025568378440214297 0.0 0.0 7.264549399209232 7.494179887649018E-4 92-93 0.0025568378440214297 0.0 0.0 7.810147736735632 7.935013998687196E-4 94-95 0.0025568378440214297 0.0 0.0 8.35662774248411 7.935013998687196E-4 96-97 0.0025568378440214297 0.0 0.0 8.919462693752102 9.257516331801728E-4 98-99 0.0027772548995405187 0.0 0.0 9.490761659952028 9.698350442839906E-4 100-101 0.0028654217217481542 2.2041705551908877E-5 0.0 10.132307541745888 9.698350442839906E-4 102-103 0.0029976719550596073 4.4083411103817754E-5 0.0 10.80250764075723 9.698350442839906E-4 104-105 0.0033503392438901493 4.4083411103817754E-5 0.0 11.467241396791698 9.698350442839906E-4 106-107 0.0035266728883054203 4.4083411103817754E-5 0.0 12.120755924700244 9.698350442839906E-4 108-109 0.0035927980049611473 4.4083411103817754E-5 0.0 12.811498893285965 9.698350442839906E-4 110-111 0.003614839710513056 4.4083411103817754E-5 0.0 13.545664021808946 9.698350442839906E-4 112-113 0.003614839710513056 6.612511665572663E-5 0.0 14.295192219101606 9.698350442839906E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1860 0.0 56.972893 1 GTATCAA 2980 0.0 46.348206 1 CAACGCA 3485 0.0 37.91289 5 ATCAACG 3485 0.0 37.74211 3 TCAACGC 3635 0.0 36.184666 4 GTACATG 9000 0.0 34.595562 1 AACGCAG 3840 0.0 34.24689 6 TATCAAC 4040 0.0 33.588467 2 ACATGGG 8815 0.0 33.353493 3 TACATGG 9420 0.0 32.60134 2 TAAGGTG 1545 0.0 29.661898 5 GTAAGGT 1505 0.0 28.868423 4 CGCAGAG 4590 0.0 28.386076 8 ACGCAGA 4670 0.0 27.90042 7 CATGGGA 5035 0.0 27.541853 4 GGTAAGG 1545 0.0 26.580141 3 CATGGGG 4600 0.0 26.394232 4 AGGTAAG 1470 0.0 25.102158 2 ATGGGAT 2785 0.0 23.934782 5 GTGTAAG 975 0.0 23.202787 1 >>END_MODULE