FastQCFastQC Report
Thu 26 May 2016
SRR1364382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268427
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.1646074570616555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.16041953300679282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.075.065871
GTATCAA58950.053.537141
ATCAACG75750.041.1791763
TCAACGC78000.039.9913144
CAACGCA79350.039.2359125
TATCAAC79700.039.2129942
AACGCAG82400.037.694746
GTACATG84850.033.8265951
ACATGGG86350.032.3325163
ACGCAGA96550.032.2291347
TACATGG90350.031.6917272
CGCAGAG98250.031.610928
GTAAGGT16050.030.04274
GGTAAGG16450.029.6740593
TAAGGTG17850.028.6806665
GTGGTAT17000.028.0223261
CATGGGA49650.027.8161964
AGGTAAG16700.027.4475292
GCAGAGT114500.026.7609129
CATGGGG42950.025.225354
Produced by FastQC (version 0.11.5)