FastQCFastQC Report
Thu 26 May 2016
SRR1364381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572419
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.053.7231671
GTATCAA26850.048.1267781
ATCAACG30400.041.705923
GTACATG94550.041.315561
TACATGG95150.040.2873542
TCAACGC31700.039.9955834
CAACGCA32850.038.5954325
ACATGGG96900.038.2697753
TATCAAC34600.037.331492
AACGCAG34300.036.7832876
CATGGGA72450.034.588794
ATGGGAT27950.029.8152125
CATGGGG33600.029.7619724
ACGCAGA43000.029.6094137
ATGGGAG31700.029.1047675
CGCAGAG45100.028.097898
GAGTACA69000.027.9619371
AGTACAT70350.025.9756222
GTATAGG4800.023.5713081
GCAGAGT53350.023.0837639
Produced by FastQC (version 0.11.5)