FastQCFastQC Report
Thu 26 May 2016
SRR1364381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572419
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.2954047235501479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.2706657703830849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.156764831765579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.076.710651
GTATCAA58600.057.4344021
TACGCCG350.007766935551.0495075
ATCAACG74000.045.0707323
TCAACGC75800.044.0790254
TATCAAC75700.044.058582
CAACGCA77250.043.097465
AACGCAG79200.042.0767256
TGGTATC12350.038.097672
GTACATG88600.036.1717761
ACGCAGA94300.035.332357
CGCAGAG95400.034.7378548
TACATGG91500.034.4979362
GTGGTAT14400.034.3349881
ACATGGG93250.032.6370123
GCAGAGT105450.030.8064929
GAGTACT61900.029.26798612-13
CATGGGA70050.029.2474924
GAGTACA69800.027.4803181
GTACTTT65900.027.13034214-15
Produced by FastQC (version 0.11.5)