FastQCFastQC Report
Thu 26 May 2016
SRR1364380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069643
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.059.27921
GTATCAA40000.053.741561
ATCAACG44650.046.385193
TCAACGC45400.045.356744
CAACGCA46250.044.5231555
AACGCAG48750.042.1178366
GTACATG113850.041.2150881
TATCAAC52500.039.9029432
TACATGG124250.037.0258182
ACATGGG122500.036.3887633
CATGGGA86200.034.7282034
ACGCAGA60250.034.0696877
TAGGCCG700.00278620834.008195
CGCAGAG60600.033.6749238
CATGGGG37200.029.7571664
ATGGGAT35700.028.6735745
GCAGAGT71000.027.73679
ATGGGAG39300.027.5613445
GTATAGA6350.026.2571371
CATATAG6950.025.689643
Produced by FastQC (version 0.11.5)