##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364380_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2069643 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34878237454479 31.0 31.0 34.0 28.0 34.0 2 31.4161273224416 33.0 31.0 34.0 28.0 34.0 3 31.508262052924103 34.0 31.0 34.0 28.0 34.0 4 34.93189405129291 37.0 35.0 37.0 32.0 37.0 5 35.01333659959713 37.0 35.0 37.0 32.0 37.0 6 35.093451382678076 37.0 35.0 37.0 32.0 37.0 7 35.03718950562971 37.0 35.0 37.0 32.0 37.0 8 34.96358454090875 37.0 35.0 37.0 32.0 37.0 9 36.53893111034125 39.0 37.0 39.0 32.0 39.0 10-11 36.45896562837166 39.0 37.0 39.0 32.0 39.0 12-13 36.47812642083683 39.0 37.0 39.0 32.0 39.0 14-15 37.81599290312387 40.0 38.0 41.0 32.0 41.0 16-17 37.825631038783015 40.0 38.0 41.0 32.0 41.0 18-19 37.845041874371574 40.0 38.0 41.0 32.0 41.0 20-21 37.87597136317713 40.0 38.0 41.0 32.0 41.0 22-23 37.83363169396848 40.0 38.0 41.0 32.0 41.0 24-25 37.848771261517086 40.0 38.0 41.0 32.0 41.0 26-27 37.81663093586671 40.0 38.0 41.0 32.0 41.0 28-29 37.81969112547429 40.0 38.0 41.0 32.0 41.0 30-31 37.7234742900104 40.0 38.0 41.0 31.5 41.0 32-33 37.68842766602742 40.0 38.0 41.0 31.5 41.0 34-35 37.67447163592948 40.0 38.0 41.0 31.5 41.0 36-37 37.61369980233306 40.0 38.0 41.0 31.0 41.0 38-39 37.532873543891384 40.0 38.0 41.0 31.0 41.0 40-41 37.475388750620276 40.0 38.0 41.0 31.0 41.0 42-43 37.35395983751787 40.0 37.5 41.0 31.0 41.0 44-45 37.24998127696419 40.0 37.0 41.0 31.0 41.0 46-47 37.15352454505439 40.0 37.0 41.0 30.5 41.0 48-49 37.01538767797152 40.0 37.0 41.0 30.0 41.0 50-51 36.911778263207715 40.0 36.5 41.0 30.0 41.0 52-53 36.76564146570206 40.0 36.0 41.0 30.0 41.0 54-55 36.582961409286526 40.0 36.0 41.0 30.0 41.0 56-57 36.405070101461945 39.0 35.0 41.0 30.0 41.0 58-59 36.19201210063765 39.0 35.0 41.0 30.0 41.0 60-61 35.95621901941543 39.0 35.0 41.0 29.0 41.0 62-63 35.70326162531413 38.0 35.0 40.5 29.0 41.0 64-65 35.37503859361252 38.0 34.5 40.0 29.0 41.0 66-67 35.07329549105812 37.0 34.0 40.0 28.5 41.0 68-69 34.750031527176425 37.0 34.0 39.5 28.0 41.0 70-71 34.39763113735074 36.0 34.0 39.0 28.0 41.0 72-73 34.02670436398934 36.0 34.0 39.0 28.0 40.5 74-75 33.65856502788162 35.0 34.0 38.0 27.5 40.0 76-77 33.41216528647695 35.0 33.5 37.0 27.5 39.0 78-79 33.23444115724306 35.0 34.0 37.0 28.0 39.0 80-81 32.94729574134283 35.0 34.0 36.5 27.0 39.0 82-83 32.63721134514503 35.0 33.0 36.0 27.0 37.0 84-85 32.36898392621336 35.0 33.0 36.0 26.0 37.0 86-87 32.14875608981839 35.0 33.0 35.0 26.0 36.5 88-89 31.877212881641906 35.0 33.0 35.0 26.0 36.0 90-91 31.7403692327614 35.0 33.0 35.0 25.0 36.0 92-93 31.58099899354623 35.0 33.0 35.0 25.0 36.0 94-95 31.4607470950304 35.0 33.0 35.0 25.0 35.5 96-97 31.340953488113648 35.0 33.0 35.0 24.5 35.0 98-99 31.191404266339653 35.0 33.0 35.0 24.0 35.0 100-101 31.083926793171575 35.0 32.0 35.0 23.5 35.0 102-103 30.05584779597254 34.0 30.5 35.0 19.5 35.0 104-105 30.738497653943217 34.5 32.0 35.0 20.0 35.0 106-107 31.053311126604925 35.0 33.0 35.0 23.0 35.0 108-109 31.069787881291603 35.0 33.0 35.0 23.0 35.0 110-111 30.981299673421937 35.0 33.0 35.0 21.0 35.0 112-113 30.86703189873809 35.0 33.0 35.0 19.5 35.0 114-115 30.740754323330158 35.0 33.0 35.0 18.5 35.0 116-117 30.582420011567212 35.0 33.0 35.0 16.0 35.0 118-119 30.37596532348816 35.0 32.0 35.0 4.5 35.0 120-121 30.158457279830387 35.0 32.0 35.0 2.0 35.0 122-123 29.93145097971003 35.0 32.0 35.0 2.0 35.0 124-125 29.080151262802328 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20720783513946017 1101 2 0.24253383781854154 1101 3 0.28751084991217013 1101 4 0.27620241902177867 1101 5 0.15065082652880335 1101 6 0.19511646463197252 1101 7 0.22317895107456565 1101 8 0.06351648489338402 1101 9 0.24683511199668828 1101 10-11 0.3068795156165294 1101 12-13 0.3889610481419936 1101 14-15 0.4253926952505509 1101 16-17 0.3515125411373603 1101 18-19 0.24334091505213706 1101 20-21 0.3697063297707146 1101 22-23 0.441329612172666 1101 24-25 0.4485207216389355 1101 26-27 0.6055052478304006 1101 28-29 0.5349523286680125 1101 30-31 0.58313850239049 1101 32-33 0.41636050514275524 1101 34-35 0.41606685347358763 1101 36-37 0.3552026129022039 1101 38-39 0.49155807319603184 1101 40-41 0.4073342370045552 1101 42-43 0.481270365289987 1101 44-45 0.5330487147730096 1101 46-47 0.5388737415574951 1101 48-49 0.4418183507220519 1101 50-51 0.44186832045782864 1101 52-53 0.3344294065415667 1101 54-55 0.40387661902227734 1101 56-57 0.33480281615933905 1101 58-59 0.35487560909951554 1101 60-61 0.2871925411645577 1101 62-63 0.25099773363595546 1101 64-65 0.36070343235682145 1101 66-67 0.3007437908919499 1101 68-69 0.2760591380571995 1101 70-71 0.31795739798350553 1101 72-73 0.39611813289329945 1101 74-75 0.41195901135507285 1101 76-77 0.34503880708687973 1101 78-79 0.4087179885511887 1101 80-81 0.40678255688476384 1101 82-83 0.3436113492660482 1101 84-85 0.33322479140323225 1101 86-87 0.270502302809156 1101 88-89 0.34632940976244697 1101 90-91 0.22362893635263958 1101 92-93 0.22608110845605722 1101 94-95 0.19538978472158774 1101 96-97 0.12196759142762659 1101 98-99 0.04253445351789509 1101 100-101 0.040241176363480236 1101 102-103 -0.13726325025153585 1101 104-105 0.0349208252272426 1101 106-107 0.02823220084399125 1101 108-109 0.08964950771578373 1101 110-111 0.051620544556381986 1101 112-113 0.008438448267135357 1101 114-115 -9.256516646800605E-4 1101 116-117 -0.004796107842757635 1101 118-119 -0.00600346495069104 1101 120-121 -0.2481254599420346 1101 122-123 -0.26404848870226516 1101 124-125 -0.22734532566087395 1102 1 0.16461336820288963 1102 2 0.18327511408613262 1102 3 0.2583915773718459 1102 4 0.33974937401610106 1102 5 0.31979296606567686 1102 6 0.33778081782264024 1102 7 0.3073651109186244 1102 8 0.30806348272985673 1102 9 0.3718257402767833 1102 10-11 0.39601662120100656 1102 12-13 0.2997221924557749 1102 14-15 0.3701946038682564 1102 16-17 0.36445957217649294 1102 18-19 0.40388383217401014 1102 20-21 0.39468999620174827 1102 22-23 0.4426342894641806 1102 24-25 0.3582322333241308 1102 26-27 0.30637065922212514 1102 28-29 0.29859286697960385 1102 30-31 0.35434201865979986 1102 32-33 0.3411551305952827 1102 34-35 0.3107702251506481 1102 36-37 0.1990931887006866 1102 38-39 0.2294310676479796 1102 40-41 0.17436666851066462 1102 42-43 0.0445508956757692 1102 44-45 0.10973348224725754 1102 46-47 0.08507699006337077 1102 48-49 0.12904991788782638 1102 50-51 0.10134018387706334 1102 52-53 0.09116365669687099 1102 54-55 0.06913217243572944 1102 56-57 0.07508568144219652 1102 58-59 0.0177902407641497 1102 60-61 0.04817686629361617 1102 62-63 0.01449234088056528 1102 64-65 -0.0037607462520981016 1102 66-67 0.08780157959447621 1102 68-69 0.023890254309371528 1102 70-71 -0.0541233112682562 1102 72-73 0.01192112863050454 1102 74-75 -0.003112454703234846 1102 76-77 0.03570577900384819 1102 78-79 0.08487151338891863 1102 80-81 0.018860293962504215 1102 82-83 -0.014982116235309206 1102 84-85 0.019849205001328585 1102 86-87 -0.016248227362432033 1102 88-89 -0.05415966013451623 1102 90-91 -0.07621857237645457 1102 92-93 -0.025373516353830894 1102 94-95 -0.11759285885316118 1102 96-97 -0.1654570331680496 1102 98-99 -0.1091066685179598 1102 100-101 -0.18423226685996497 1102 102-103 -0.33452188829964413 1102 104-105 -0.22293870072438793 1102 106-107 -0.2449440624638477 1102 108-109 -0.20747484697436036 1102 110-111 -0.22250159305259842 1102 112-113 -0.2702376758037204 1102 114-115 -0.29791947168481414 1102 116-117 -0.32954226811523313 1102 118-119 -0.48196165636941046 1102 120-121 -0.5863903261674253 1102 122-123 -0.6109594359421564 1102 124-125 -0.7373295543951031 1103 1 0.2573083713324884 1103 2 0.35275661325579577 1103 3 0.26889835576348275 1103 4 0.32208114435760393 1103 5 0.21964933001122233 1103 6 0.3123767735812848 1103 7 0.23994118918398755 1103 8 0.2983997068744628 1103 9 0.3266993260767137 1103 10-11 0.4963796007265344 1103 12-13 0.3542193215437095 1103 14-15 0.4322945846926558 1103 16-17 0.3536628985993744 1103 18-19 0.44432375829369875 1103 20-21 0.5088487755485502 1103 22-23 0.4578883841771457 1103 24-25 0.5223891132016973 1103 26-27 0.5568826114061523 1103 28-29 0.5320882883542382 1103 30-31 0.6712969523954584 1103 32-33 0.5674085993879032 1103 34-35 0.6068958958441897 1103 36-37 0.5545528512102251 1103 38-39 0.4609069461977242 1103 40-41 0.42771719656311546 1103 42-43 0.43542887558119503 1103 44-45 0.4659226313935747 1103 46-47 0.4734015406127696 1103 48-49 0.46448465212494483 1103 50-51 0.4033126017747648 1103 52-53 0.48103582241741805 1103 54-55 0.40176925984387424 1103 56-57 0.34925733195434105 1103 58-59 0.3527261382000475 1103 60-61 0.25620673680408146 1103 62-63 0.2467524288712113 1103 64-65 0.33344207726369035 1103 66-67 0.22748402451890826 1103 68-69 0.21033559662991053 1103 70-71 0.38356346987309564 1103 72-73 0.3522151121973778 1103 74-75 0.21356271434952845 1103 76-77 0.26464043907121493 1103 78-79 0.32636076058403773 1103 80-81 0.3438242040470598 1103 82-83 0.26924337970706347 1103 84-85 0.19775892051511335 1103 86-87 0.27314337264191835 1103 88-89 0.39306765748930417 1103 90-91 0.24468612672953327 1103 92-93 0.22171079837194085 1103 94-95 0.19004336927128307 1103 96-97 0.19962027522977266 1103 98-99 0.18558309388557248 1103 100-101 0.14690130019853598 1103 102-103 -0.04950065042459073 1103 104-105 0.18308980759529092 1103 106-107 0.08353951888589606 1103 108-109 0.04263731216136435 1103 110-111 0.07267773493327923 1103 112-113 0.011232006858282517 1103 114-115 -0.05393943239293719 1103 116-117 -0.14337913265232416 1103 118-119 -0.0856482547999029 1103 120-121 -0.2163710058517907 1103 122-123 -0.2567303581435141 1103 124-125 -0.2485346138575366 1104 1 0.32068548877074576 1104 2 0.2978148887864158 1104 3 0.3001895713076195 1104 4 0.3533537482064588 1104 5 0.30402792845585225 1104 6 0.23762229377926758 1104 7 0.3384488175562339 1104 8 0.27616897295254006 1104 9 0.3679821586489922 1104 10-11 0.44360399125408634 1104 12-13 0.38673230127391633 1104 14-15 0.4736895279501141 1104 16-17 0.467759528759224 1104 18-19 0.4891474934252713 1104 20-21 0.34749699600617134 1104 22-23 0.33233917397379287 1104 24-25 0.4767367721808071 1104 26-27 0.45663660664332184 1104 28-29 0.4545701369955637 1104 30-31 0.41325901272994514 1104 32-33 0.4238463517470734 1104 34-35 0.3906499672901944 1104 36-37 0.30740963630747586 1104 38-39 0.29685955443409284 1104 40-41 0.355492861099755 1104 42-43 0.3002034009499326 1104 44-45 0.23521993086963278 1104 46-47 0.30288961326930774 1104 48-49 0.286436419861559 1104 50-51 0.2202650919535145 1104 52-53 0.2385166777008081 1104 54-55 0.18530924172995356 1104 56-57 0.09234049914066134 1104 58-59 0.1267361612036595 1104 60-61 -0.007662280031468072 1104 62-63 0.17141090976922158 1104 64-65 0.1303970717616849 1104 66-67 0.22678746255055415 1104 68-69 0.09083700451103738 1104 70-71 -0.05507580816052382 1104 72-73 0.013992152457667828 1104 74-75 0.07934133517225206 1104 76-77 0.04524638080538068 1104 78-79 0.10549604800587531 1104 80-81 0.07924794279084324 1104 82-83 0.04865872358511325 1104 84-85 -0.023057060257357875 1104 86-87 -0.004803629619679839 1104 88-89 0.01631485428166002 1104 90-91 -0.014104044797612403 1104 92-93 -0.024951761240551207 1104 94-95 -0.027115798827786364 1104 96-97 -0.12544118741600485 1104 98-99 -0.09546082839374748 1104 100-101 -0.21304260921779417 1104 102-103 -0.40755398603684867 1104 104-105 -0.12438404352348087 1104 106-107 -0.09784571325150182 1104 108-109 -0.1078291035300829 1104 110-111 -0.09678857855903544 1104 112-113 -0.26341813732178565 1104 114-115 -0.3037956047359103 1104 116-117 -0.393848004206518 1104 118-119 -0.43322133378661576 1104 120-121 -0.46146632856757464 1104 122-123 -0.5674407720588093 1104 124-125 -0.5386347778166574 1105 1 0.18723360833533675 1105 2 0.20909004793145414 1105 3 0.08811631546608822 1105 4 0.29237373577299053 1105 5 0.235127930657967 1105 6 0.3188891695902427 1105 7 0.3272262973899558 1105 8 0.2201585374761592 1105 9 0.27691577255414757 1105 10-11 0.2828986818740944 1105 12-13 0.3276977183541874 1105 14-15 0.4090620006015655 1105 16-17 0.3196719053985291 1105 18-19 0.24351307727327054 1105 20-21 0.38117472010508635 1105 22-23 0.29254650929806303 1105 24-25 0.30504566981981185 1105 26-27 0.32608511064609047 1105 28-29 0.3083391175187131 1105 30-31 0.3475941629479564 1105 32-33 0.38077576723843265 1105 34-35 0.20557467407032703 1105 36-37 0.16086555677391345 1105 38-39 0.15245560087835486 1105 40-41 0.17645617558525117 1105 42-43 0.2569088996269713 1105 44-45 0.1711313390025495 1105 46-47 0.23482673615739458 1105 48-49 0.13664035884946912 1105 50-51 0.27373060431753515 1105 52-53 0.2751681017905767 1105 54-55 0.30225829414364114 1105 56-57 0.20697918527513792 1105 58-59 0.18874417943715827 1105 60-61 0.09531859800195264 1105 62-63 0.09098999485888726 1105 64-65 0.06276392436576117 1105 66-67 0.010220042732889567 1105 68-69 -0.001731424043931895 1105 70-71 0.003995723680333185 1105 72-73 -0.05700260566558768 1105 74-75 -0.04260635574250671 1105 76-77 0.04842716414791681 1105 78-79 0.22025405057049596 1105 80-81 0.19326304971191632 1105 82-83 0.15766677527379613 1105 84-85 0.12364370811080505 1105 86-87 0.021223338646173318 1105 88-89 0.003567211813397364 1105 90-91 0.03994036928778044 1105 92-93 0.05536010671057312 1105 94-95 0.02412928453067309 1105 96-97 0.00987634885958144 1105 98-99 0.011376500646026244 1105 100-101 -0.061095041113205184 1105 102-103 -0.28522304270230947 1105 104-105 -0.1663589707690427 1105 106-107 -0.17250042611286176 1105 108-109 -0.10556029155329227 1105 110-111 -0.1275577132301251 1105 112-113 -0.24387210510656132 1105 114-115 -0.265942970161376 1105 116-117 -0.22964678694601304 1105 118-119 -0.288600080343695 1105 120-121 -0.36942397218771106 1105 122-123 -0.42985860570438916 1105 124-125 -0.5268112818600805 1106 1 0.2942245173970228 1106 2 0.30642866609597164 1106 3 0.32258850741732914 1106 4 0.2921902012046047 1106 5 0.27801946705226044 1106 6 0.27853888879339905 1106 7 0.20060030367131532 1106 8 0.29842332310938957 1106 9 0.38751478839900244 1106 10-11 0.3892096491042878 1106 12-13 0.25935771268038366 1106 14-15 0.3995201619528572 1106 16-17 0.4169260896250435 1106 18-19 0.3837010399772822 1106 20-21 0.28334607368407916 1106 22-23 0.38691909029839167 1106 24-25 0.36928030756455854 1106 26-27 0.3944237099247232 1106 28-29 0.4235044975347222 1106 30-31 0.5488201276546718 1106 32-33 0.432823438408775 1106 34-35 0.4327265289849933 1106 36-37 0.4824665407683355 1106 38-39 0.3980690041456967 1106 40-41 0.46683639250764486 1106 42-43 0.4292611196418221 1106 44-45 0.4227289034475916 1106 46-47 0.4196075715722003 1106 48-49 0.37732324205598644 1106 50-51 0.2787306404819887 1106 52-53 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106-107 -0.5007763434046559 2216 108-109 -0.5041090958425016 2216 110-111 -0.5248748362480917 2216 112-113 -0.553296201154339 2216 114-115 -0.42816929491648636 2216 116-117 -0.49688925472416656 2216 118-119 -0.43217097696272333 2216 120-121 -0.23140366643329102 2216 122-123 -0.25184374634771345 2216 124-125 -0.1979702606765521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23737.0 3 5126.0 4 3553.0 5 3003.0 6 3424.0 7 4088.0 8 4088.0 9 4271.0 10 4753.0 11 5370.0 12 5940.0 13 5968.0 14 6176.0 15 5996.0 16 5606.0 17 5293.0 18 4896.0 19 4801.0 20 4932.0 21 5230.0 22 6286.0 23 8308.0 24 12192.0 25 17527.0 26 21801.0 27 21728.0 28 25592.0 29 31685.0 30 37936.0 31 46415.0 32 60013.0 33 80845.0 34 113081.0 35 171241.0 36 330878.0 37 655962.0 38 311198.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97922411897068 19.451406529079225 12.66671339386923 23.902655958080864 2 15.393242627470856 21.14702632022742 39.27840566173127 24.18132539057045 3 17.99485985395182 26.54403696189247 29.874646729716158 25.58645645443955 4 12.028700069870531 16.951238486583023 38.089828570765896 32.93023287278054 5 13.014525182863618 39.510962151399234 34.40924237554762 13.06527029018952 6 31.167219903375294 37.36986693319981 18.172327596039896 13.290585567385005 7 28.308794573592845 33.13425941361134 21.96877341957293 16.588172593222886 8 26.47517129782411 35.4030964790512 20.596180690274306 17.525551532850383 9 27.30292132507877 16.166218038569937 20.0573722134687 36.473488422882596 10-11 24.348202333592557 27.42917070420167 28.31763375444491 19.90499320776086 12-13 24.845521792920806 25.202204163694113 28.04793227383562 21.90434176954946 14-15 22.378011790959427 26.22102963491415 25.445144594231884 25.955813979894536 16-17 22.33133411189101 29.56723370597481 26.131441280339228 21.96999090179495 18-19 22.371501931251878 28.417378464488063 27.437909551506976 21.773210052753083 20-21 23.0501458706385 27.996846797658726 26.957536617443985 21.995470714258794 22-23 23.14103115418029 27.94553357537197 26.649053314534594 22.264381955913148 24-25 22.748150284855893 28.108760786280534 26.874876488360556 22.268212440503024 26-27 22.540033493715338 28.33287109558078 26.723389842301227 22.40370556840265 28-29 22.336061266767732 27.976420991598772 27.245399254700402 22.4421184869331 30-31 22.483196344869803 27.726961078719526 27.164544154801877 22.625298421608793 32-33 22.422546916371928 27.975517481301093 26.869987050888078 22.731948551438897 34-35 22.985784394965854 27.845935523647498 26.689132872735037 22.479147208651607 36-37 23.150016427977814 27.422474439999228 26.58264239191357 22.84486674010939 38-39 22.58411545386747 27.721481097804183 26.955671770048955 22.738731678279393 40-41 22.76296745293309 27.334936354673424 27.28461343518067 22.617482757212812 42-43 22.76978813542943 27.3519006350912 26.99222177554957 22.886089453929795 44-45 22.35341412615731 27.617437933489548 27.049965102196566 22.979182838156575 46-47 22.736589226542563 27.773523150380598 26.7140162404899 22.77587138258694 48-49 22.60645918160765 27.71101102943841 26.768288057408935 22.914241731545005 50-51 22.474262469421056 27.343580511228264 27.131128412001495 23.05102860734919 52-53 22.78505783309444 27.467850974539036 26.87824984254352 22.868841349823 54-55 22.24289464866015 27.40538016205668 27.147236558835843 23.20448863044732 56-57 22.345083036140085 27.740704788898174 27.14709942448956 22.767112750472183 58-59 22.609645202282532 27.072085348588875 27.035387967897645 23.282881481230945 60-61 22.38541374745122 27.48741314830742 27.199195987669235 22.927977116572123 62-63 22.456480695197566 27.475717248635323 27.164525983540518 22.903276072626596 64-65 22.19704572501789 27.339388899653123 27.18421481138243 23.279350563946558 66-67 22.19865876751374 27.566101288709984 26.959217506471088 23.276022437305187 68-69 22.27851128854958 27.266105359796093 27.29243926893203 23.162944082722298 70-71 22.138333509695318 27.279092117488972 27.31308407828153 23.26949029453418 72-73 22.349698127885542 27.30523269157826 26.91032260090791 23.434746579628282 74-75 22.19482031470264 27.5535494825219 26.96312266425783 23.288507538517624 76-77 22.34635143933601 27.20512365207581 27.324541853748336 23.123983054839844 78-79 22.387442736270113 27.289908972352507 27.203249337671064 23.119398953706312 80-81 22.296667039521573 27.562062826615463 27.076441456666007 23.064828677196957 82-83 22.51118677677325 27.317357538614374 27.064800440271718 23.106655244340658 84-85 22.397919050343248 27.14905355154926 27.076117879893623 23.376909518213864 86-87 22.397975454194047 26.894158291457288 27.400705450328566 23.3071608040201 88-89 22.684184331708167 26.894198199591212 27.195377694452365 23.22623977424826 90-91 22.399118685178788 27.153169520229415 27.398502634522515 23.049209160069285 92-93 22.534265323393672 26.890641597992676 27.30146550951466 23.273627569098988 94-95 22.46190367129843 26.62292921122794 27.195473376297702 23.719693741175927 96-97 22.757283457956547 26.561208636194195 27.221831252172784 23.459676653676475 98-99 22.55188670242669 26.633921675353005 27.15418387687265 23.66000774534766 100-101 22.195376093169088 26.676937505481096 27.48496277203449 23.64272362931533 102-103 22.62527547340839 26.531042670172628 27.128640585537724 23.715041270881258 104-105 22.45828473357022 26.472898968219376 27.41089452876993 23.657921769440467 106-107 22.5274340302777 26.448869539166274 27.41226183975917 23.611434590796858 108-109 22.462021125292296 26.828000229029264 26.832711274062266 23.87726737161617 110-111 22.278631105072403 26.878360232913334 27.204919185296173 23.63808947671809 112-113 22.34368190089457 26.880175532012558 27.449272659275202 23.326869907817667 114-115 22.299140599406066 27.12632309326231 27.072495798668523 23.502040508663104 116-117 22.082711235450155 27.014969003821975 27.273761723223217 23.62855803750465 118-119 22.181565494821566 27.27400389137975 26.865989099302695 23.678441514495994 120-121 22.062103463247706 27.296732895960496 27.104575888245954 23.536587752545845 122-123 22.23039895475266 27.38123309421625 27.08199045400032 23.306377497030766 124-125 22.319461090936677 27.483506987016987 26.930450183695935 23.2665817383504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1093.0 1 1010.0 2 884.5 3 719.5 4 566.0 5 480.5 6 400.5 7 380.5 8 373.0 9 362.0 10 346.5 11 290.5 12 285.5 13 311.5 14 304.0 15 331.5 16 388.0 17 463.5 18 606.5 19 748.0 20 906.0 21 1274.5 22 1792.5 23 2414.5 24 3317.0 25 4683.0 26 6816.0 27 9314.0 28 11746.0 29 15465.5 30 21247.0 31 26408.5 32 31459.0 33 37610.5 34 43863.5 35 48841.0 36 56780.0 37 65857.0 38 68696.5 39 69845.5 40 69558.5 41 71243.5 42 74067.5 43 76051.5 44 79790.0 45 83154.5 46 81150.5 47 75936.5 48 74847.0 49 74510.5 50 72453.5 51 69232.0 52 66849.0 53 67456.5 54 67677.5 55 62269.5 56 55997.5 57 51210.0 58 48026.5 59 43267.0 60 35261.5 61 29062.0 62 24344.0 63 19593.5 64 15307.0 65 11738.0 66 8339.0 67 6742.0 68 5110.5 69 3029.0 70 2030.0 71 1541.0 72 1271.0 73 985.5 74 777.0 75 659.5 76 455.0 77 200.5 78 93.5 79 55.5 80 30.0 81 21.0 82 19.0 83 15.0 84 12.0 85 9.5 86 6.0 87 7.0 88 6.0 89 5.0 90 6.5 91 4.5 92 4.5 93 5.0 94 3.5 95 1.5 96 1.5 97 2.0 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043389125564167345 2 0.008213976999898051 3 0.0025125106117335214 4 0.004880068688174724 5 0.02309577062324275 6 0.02932872964081245 7 0.0028990507058463707 8 9.663502352821236E-5 9 0.0 10-11 7.247626764615927E-5 12-13 7.006039205795395E-4 14-15 3.140638264666902E-4 16-17 1.9327004705642473E-4 18-19 4.831751176410618E-5 20-21 4.831751176410618E-5 22-23 4.831751176410618E-5 24-25 0.0 26-27 4.831751176410618E-5 28-29 2.65746314702584E-4 30-31 1.9327004705642473E-4 32-33 1.4495253529231853E-4 34-35 7.730801882256989E-4 36-37 1.4495253529231853E-4 38-39 1.2079377941026545E-4 40-41 4.590163617590086E-4 42-43 9.180327235180172E-4 44-45 0.0013770490852770259 46-47 4.831751176410618E-5 48-49 0.0 50-51 0.0 52-53 0.001135461526456495 54-55 0.0028265744382002115 56-57 9.663502352821236E-5 58-59 6.281276529333803E-4 60-61 0.001111302770574442 62-63 7.489214323436457E-4 64-65 0.0015461603764513978 66-67 0.0026091456352617336 68-69 0.0032855907999592197 70-71 0.0017394304235078225 72-73 0.002802415682318158 74-75 0.0018602242029180879 76-77 0.0012320965499847075 78-79 0.0025125106117335214 80-81 0.0012804140617488139 82-83 5.798101411692741E-4 84-85 5.31492629405168E-4 86-87 0.0020776530058565657 88-89 0.005459878829343998 90-91 3.8654009411284945E-4 92-93 0.0010388265029282829 94-95 0.0010146677470462297 96-97 2.174288029384778E-4 98-99 6.522864088154334E-4 100-101 0.0014253665970411322 102-103 0.0011596202823385483 104-105 8.213976999898049E-4 106-107 0.006426229064626121 108-109 0.0018602242029180879 110-111 0.0015461603764513978 112-113 0.002367558076441203 114-115 0.0029473682176104766 116-117 0.001594477888215504 118-119 0.04677135138765478 120-121 0.011668679091031642 122-123 0.0041069884999490255 124-125 0.0026333043911437866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2069643.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.68289654767625 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86226312602136 34.791114482279596 2 10.648139174191783 9.515794022913406 3 4.014993607129128 5.38204631960797 4 2.018628360558257 3.607926488121197 5 1.2073684259654696 2.6974359236172893 6 0.8007847378838032 2.1468828959892012 7 0.5289424881727443 1.654427773508524 8 0.3934151080391966 1.4063141258246636 9 0.31198403666912583 1.2546315391511684 >10 1.8411686254325816 15.810904313538934 >50 0.21673191904814024 6.769795202479326 >100 0.14562552127042283 11.820063696706057 >500 0.008817170233148186 2.4335051465948756 >1k 0.0011376993849223464 0.7091580696678195 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.247626764615927E-5 0.0 22-23 0.0 0.0 0.0 1.2079377941026545E-4 0.0 24-25 0.0 0.0 0.0 5.073338735231148E-4 0.0 26-27 0.0 0.0 0.0 0.001352890329394973 0.0 28-29 0.0 0.0 0.0 0.005266608782287573 0.0 30-31 0.0 0.0 0.0 0.019979291114457905 0.0 32-33 0.0 0.0 0.0 0.05138567376112692 0.0 34-35 0.0 0.0 0.0 0.09610353089880719 0.0 36-37 0.0 0.0 0.0 0.17312164465079244 0.0 38-39 0.0 0.0 0.0 0.285314906967047 0.0 40-41 0.0 0.0 0.0 0.4111578663566615 0.0 42-43 0.0 0.0 0.0 0.5342225688198399 0.0 44-45 0.0 0.0 0.0 0.6823399011327074 0.0 46-47 0.0 0.0 0.0 0.8639895866098646 0.0 48-49 0.0 0.0 0.0 1.0611491933633 0.0 50-51 0.0 0.0 0.0 1.2816944758105624 0.0 52-53 0.0 0.0 0.0 1.4920689220314807 0.0 54-55 0.0 0.0 0.0 1.7136047134699077 0.0 56-57 0.0 0.0 0.0 1.9764519774666454 0.0 58-59 0.0 0.0 0.0 2.26456930011601 0.0 60-61 0.0 0.0 0.0 2.578850555385639 0.0 62-63 0.0 0.0 0.0 2.897359592934627 0.0 64-65 0.0 0.0 0.0 3.2153129790983277 0.0 66-67 0.0 0.0 0.0 3.5770420309203086 0.0 68-69 0.0 0.0 0.0 3.9799134440094255 0.0 70-71 0.0 0.0 0.0 4.434895293536131 0.0 72-73 0.0 0.0 0.0 4.8885725702452065 0.0 74-75 0.0 0.0 0.0 5.344158388668964 0.0 76-77 0.0 0.0 0.0 5.818104861563081 0.0 78-79 0.0 0.0 0.0 6.34655348772711 0.0 80-81 0.0 0.0 0.0 6.934336018337461 0.0 82-83 0.0 0.0 0.0 7.532627607756507 0.0 84-85 0.0 0.0 0.0 8.124106428016812 0.0 86-87 0.0 0.0 0.0 8.726939863541684 0.0 88-89 0.0 0.0 0.0 9.375940681557157 0.0 90-91 0.0 0.0 0.0 10.088406551274785 0.0 92-93 0.0 0.0 0.0 10.821238252200983 0.0 94-95 0.0 0.0 0.0 11.558176941627131 0.0 96-97 0.0 0.0 0.0 12.303595354367879 0.0 98-99 0.0 0.0 0.0 13.065248451061368 0.0 100-101 0.0 0.0 0.0 13.900126736833357 0.0 102-103 0.0 0.0 0.0 14.774770334787208 0.0 104-105 0.0 0.0 0.0 15.652361300958667 0.0 106-107 0.0 0.0 0.0 16.517148126512637 0.0 108-109 0.0 0.0 0.0 17.379736505281343 0.0 110-111 0.0 0.0 0.0 18.30327742514047 0.0 112-113 0.0 0.0 0.0 19.2689512152579 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2220 0.0 59.2792 1 GTATCAA 4000 0.0 53.74156 1 ATCAACG 4465 0.0 46.38519 3 TCAACGC 4540 0.0 45.35674 4 CAACGCA 4625 0.0 44.523155 5 AACGCAG 4875 0.0 42.117836 6 GTACATG 11385 0.0 41.215088 1 TATCAAC 5250 0.0 39.902943 2 TACATGG 12425 0.0 37.025818 2 ACATGGG 12250 0.0 36.388763 3 CATGGGA 8620 0.0 34.728203 4 ACGCAGA 6025 0.0 34.069687 7 TAGGCCG 70 0.002786208 34.00819 5 CGCAGAG 6060 0.0 33.674923 8 CATGGGG 3720 0.0 29.757166 4 ATGGGAT 3570 0.0 28.673574 5 GCAGAGT 7100 0.0 27.7367 9 ATGGGAG 3930 0.0 27.561344 5 GTATAGA 635 0.0 26.257137 1 CATATAG 695 0.0 25.68964 3 >>END_MODULE