FastQCFastQC Report
Thu 26 May 2016
SRR1364380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069643
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48490.23429161454415087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.20462466232098966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.13142363199836882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.076.781461
GTATCAA64000.057.794471
ATCAACG83150.043.895033
TCAACGC84600.043.072314
CAACGCA85250.042.74395
AACGCAG88500.041.351056
TATCAAC90500.040.3300742
GTACATG110950.038.7063871
TACATGG121050.035.611552
ACATGGG120200.034.9222153
ACGCAGA105950.034.4232037
CGCAGAG106600.034.157498
CATGGGA84300.032.277944
GCAGAGT118800.030.2991669
CATGGGG39400.027.8060154
GAGTACT59750.027.33355912-13
CATATAG7550.026.813233
ATGGGAG36850.026.3370555
GAGTACA85400.026.2243441
CAGAGTA116100.025.90482510-11
Produced by FastQC (version 0.11.5)