##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364380_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2069643 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29336991935324 33.0 31.0 34.0 30.0 34.0 2 32.30864791657305 33.0 31.0 34.0 30.0 34.0 3 32.432278417098985 34.0 31.0 34.0 30.0 34.0 4 36.03865932433758 37.0 35.0 37.0 35.0 37.0 5 35.9732214686301 37.0 35.0 37.0 35.0 37.0 6 36.0335627931967 37.0 35.0 37.0 35.0 37.0 7 35.988755548662255 37.0 35.0 37.0 35.0 37.0 8 35.99336793833526 37.0 35.0 37.0 35.0 37.0 9 37.74594459044386 39.0 38.0 39.0 35.0 39.0 10-11 37.70752081397613 39.0 37.5 39.0 35.0 39.0 12-13 37.677653585666704 39.0 37.0 39.0 35.0 39.0 14-15 39.07322832971677 41.0 38.0 41.0 36.0 41.0 16-17 39.0750890370948 41.0 38.0 41.0 35.5 41.0 18-19 39.14231536550024 41.0 38.5 41.0 36.0 41.0 20-21 39.150741697964335 41.0 39.0 41.0 35.5 41.0 22-23 39.1092103807275 41.0 39.0 41.0 35.0 41.0 24-25 39.07255067661428 41.0 39.0 41.0 35.0 41.0 26-27 38.98719610097007 41.0 39.0 41.0 35.0 41.0 28-29 38.90026661602991 40.5 38.0 41.0 35.0 41.0 30-31 38.790301998943775 40.0 38.0 41.0 35.0 41.0 32-33 38.67541576010935 40.0 38.0 41.0 34.5 41.0 34-35 38.57702173756537 40.0 38.0 41.0 34.0 41.0 36-37 38.46012428230376 40.0 38.0 41.0 34.0 41.0 38-39 38.38464508130146 40.0 38.0 41.0 34.0 41.0 40-41 38.28015507988576 40.0 38.0 41.0 34.0 41.0 42-43 38.1348179371998 40.0 38.0 41.0 33.0 41.0 44-45 37.97167651619144 40.0 38.0 41.0 33.0 41.0 46-47 37.74598662667909 40.0 37.5 41.0 33.0 41.0 48-49 37.54612220561711 40.0 37.0 41.0 33.0 41.0 50-51 37.406986373978505 40.0 37.0 41.0 32.0 41.0 52-53 37.26840740166299 40.0 37.0 41.0 32.0 41.0 54-55 37.09421818158977 40.0 36.0 41.0 31.5 41.0 56-57 36.91418761593183 39.5 36.0 41.0 31.0 41.0 58-59 36.707381176367136 39.0 35.5 41.0 31.0 41.0 60-61 36.4676011756617 39.0 35.0 41.0 31.0 41.0 62-63 36.24695249373926 39.0 35.0 41.0 31.0 41.0 64-65 35.96105125376695 38.0 35.0 40.5 30.0 41.0 66-67 35.637983942158144 37.0 35.0 40.0 30.0 41.0 68-69 35.30682876225514 37.0 35.0 39.5 29.5 41.0 70-71 35.05091626913433 36.5 34.5 39.0 29.5 41.0 72-73 34.84013402311413 36.0 35.0 39.0 30.0 41.0 74-75 34.49445991410113 35.5 34.0 38.5 29.5 40.5 76-77 34.15974397516867 35.0 34.0 37.0 29.0 39.5 78-79 33.83466158173172 35.0 34.0 37.0 29.0 39.0 80-81 33.528381947997794 35.0 34.0 36.5 29.0 39.0 82-83 33.24298876666169 35.0 34.0 36.0 29.0 37.0 84-85 33.00048220876741 35.0 34.0 36.0 29.0 37.0 86-87 32.808528330731434 35.0 34.0 35.0 29.0 37.0 88-89 32.64469572771729 35.0 34.0 35.0 29.0 36.0 90-91 32.498668610963335 35.0 34.0 35.0 28.5 36.0 92-93 32.377637109395195 35.0 33.0 35.0 27.5 36.0 94-95 32.28523035132146 35.0 33.0 35.0 27.0 35.5 96-97 32.199806198460315 35.0 33.0 35.0 27.0 35.0 98-99 32.13290093992056 35.0 33.0 35.0 27.0 35.0 100-101 32.069071332592145 35.0 33.0 35.0 27.0 35.0 102-103 31.07556713887371 34.0 31.5 35.0 24.5 35.0 104-105 31.80029140291345 35.0 33.0 35.0 25.5 35.0 106-107 32.09299429901679 35.0 33.0 35.0 27.0 35.0 108-109 32.097042340152385 35.0 34.0 35.0 27.0 35.0 110-111 32.06027875338887 35.0 34.0 35.0 27.0 35.0 112-113 31.98578281375097 35.0 33.5 35.0 27.0 35.0 114-115 31.898856952624197 35.0 33.0 35.0 27.0 35.0 116-117 31.826298786795597 35.0 33.0 35.0 27.0 35.0 118-119 31.7042332904757 35.0 33.0 35.0 25.0 35.0 120-121 31.552913715070666 35.0 33.0 35.0 25.0 35.0 122-123 31.384383683562817 35.0 33.0 35.0 25.0 35.0 124-125 30.67797610505773 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12492728272803788 1101 2 0.16887623590547207 1101 3 0.19867404845506798 1101 4 0.13914612966009798 1101 5 0.18640449805926806 1101 6 0.2200344611637277 1101 7 0.2558812434193669 1101 8 0.2076137076217961 1101 9 0.25166818712285277 1101 10-11 0.2520368224023741 1101 12-13 0.17051695418490453 1101 14-15 0.3031328003335574 1101 16-17 0.32548688481028165 1101 18-19 0.3054296280535169 1101 20-21 0.29268508134999394 1101 22-23 0.27765649340246057 1101 24-25 0.30893233805478104 1101 26-27 0.2863206018008171 1101 28-29 0.27699291051727926 1101 30-31 0.32676250326065315 1101 32-33 0.1950957535417146 1101 34-35 0.30338034682731774 1101 36-37 0.4085146432106299 1101 38-39 0.37581522917093224 1101 40-41 0.27597166834497244 1101 42-43 0.3111134695846616 1101 44-45 0.31343214249408646 1101 46-47 0.30240773861666526 1101 48-49 0.23813877714775344 1101 50-51 0.22441080524399126 1101 52-53 0.20143379420980523 1101 54-55 0.2792192332680088 1101 56-57 0.3207175649311509 1101 58-59 0.10704562223815373 1101 60-61 0.1996989616639837 1101 62-63 0.049977077228327005 1101 64-65 -0.002738363606738403 1101 66-67 0.029873740623358458 1101 68-69 0.032889327341521835 1101 70-71 0.03688468700412528 1101 72-73 -0.004710123318574233 1101 74-75 0.049057165013906 1101 76-77 0.01167583199413258 1101 78-79 -0.24446651709921952 1101 80-81 -0.22489938621615835 1101 82-83 -0.15401726184908426 1101 84-85 -0.3580592777688665 1101 86-87 -0.300101316503671 1101 88-89 -0.33222742228657864 1101 90-91 -0.714847902100086 1101 92-93 -0.5654230145548347 1101 94-95 -0.39382526414765096 1101 96-97 -0.298559609653374 1101 98-99 -0.281159968235265 1101 100-101 -0.3154070068968693 1101 102-103 -0.2089730738521176 1101 104-105 -0.18008956602318094 1101 106-107 -0.137213287316456 1101 108-109 -0.10750916279472733 1101 110-111 -0.15807078251565088 1101 112-113 -0.22363988877512497 1101 114-115 -0.2659847484282025 1101 116-117 -0.1961515760605188 1101 118-119 -0.23830260431210348 1101 120-121 -0.2399807616605223 1101 122-123 -0.10772657009551168 1101 124-125 -0.19151395142979766 1102 1 0.09322409313011093 1102 2 0.11305425574138894 1102 3 0.02295430036689794 1102 4 0.07821120688306848 1102 5 0.02400731921471788 1102 6 0.08117770388591339 1102 7 0.14477280994250918 1102 8 0.21571622888221498 1102 9 0.2576513608691826 1102 10-11 0.27446334811837403 1102 12-13 0.26786636403431885 1102 14-15 0.35612786813125297 1102 16-17 0.3328697918642334 1102 18-19 0.34652261158070985 1102 20-21 0.266246120700508 1102 22-23 0.31760947414491625 1102 24-25 0.3408745065100973 1102 26-27 0.3897234732957955 1102 28-29 0.35973314786278365 1102 30-31 0.41130077200945436 1102 32-33 0.2518267905359153 1102 34-35 0.28663442218665836 1102 36-37 0.36417636696445754 1102 38-39 0.3693614612040079 1102 40-41 0.3197677236541878 1102 42-43 0.3582505430375278 1102 44-45 0.36060268637515946 1102 46-47 0.41224496523020804 1102 48-49 0.30815288828028997 1102 50-51 0.2023450907440676 1102 52-53 0.17592464205591796 1102 54-55 0.2283107119647454 1102 56-57 0.2088928642906538 1102 58-59 0.23788018728324545 1102 60-61 0.2740059899183791 1102 62-63 0.24648664709315682 1102 64-65 0.14894760880292068 1102 66-67 0.17788198238159225 1102 68-69 0.03545249273362572 1102 70-71 0.08795252754564586 1102 72-73 0.1899117143953717 1102 74-75 0.1374686317148459 1102 76-77 0.16292267238571156 1102 78-79 0.14378107838521714 1102 80-81 0.19242999492901447 1102 82-83 0.20851098719231942 1102 84-85 0.20104615140235182 1102 86-87 0.18469909263884432 1102 88-89 0.139646038073721 1102 90-91 0.13739086790184274 1102 92-93 0.219672737646178 1102 94-95 0.16750929554217464 1102 96-97 0.21686490248166024 1102 98-99 0.143583782166381 1102 100-101 0.13154972788881025 1102 102-103 0.21742813215434964 1102 104-105 0.14475633612535077 1102 106-107 0.10923282261649803 1102 108-109 0.17984517390171817 1102 110-111 0.2088969841231645 1102 112-113 0.050363763518006976 1102 114-115 -0.015529550604583164 1102 116-117 -0.02127996862312287 1102 118-119 -0.14923110076691515 1102 120-121 -0.07450698105516551 1102 122-123 -0.23860129965445154 1102 124-125 -0.49439120332020536 1103 1 0.08751257004522728 1103 2 0.11817106405418798 1103 3 0.19100350928318477 1103 4 0.08639565800881854 1103 5 -0.024351810719828393 1103 6 0.03469678722015601 1103 7 0.0975198947094924 1103 8 0.11099080168254005 1103 9 0.1730788499879381 1103 10-11 0.1915413403251165 1103 12-13 0.1700798788483695 1103 14-15 0.2537197933970958 1103 16-17 0.2742989796511921 1103 18-19 0.23126734100583946 1103 20-21 0.1431397106250003 1103 22-23 0.19424957537758303 1103 24-25 0.15867018150910184 1103 26-27 0.18594886612412154 1103 28-29 0.24521052970674617 1103 30-31 0.203174797400834 1103 32-33 0.18328851351731856 1103 34-35 0.17242973900003022 1103 36-37 0.2029921564381425 1103 38-39 0.1524843604814805 1103 40-41 0.14031740291523676 1103 42-43 0.11265821616962057 1103 44-45 0.12114293160567513 1103 46-47 0.1553584847477083 1103 48-49 0.014059649667153451 1103 50-51 -0.11018543644004808 1103 52-53 0.07909037888717307 1103 54-55 0.13648177635494818 1103 56-57 0.08787609649986905 1103 58-59 0.03360810007409043 1103 60-61 0.026384849006710454 1103 62-63 0.05130736433274308 1103 64-65 0.01666450105417283 1103 66-67 -0.015938049240048713 1103 68-69 -0.08946384016915943 1103 70-71 -0.01788182788303061 1103 72-73 0.025530968500937945 1103 74-75 0.07934125511703627 1103 76-77 0.03592642067167162 1103 78-79 0.13472603719387166 1103 80-81 0.07016636126245857 1103 82-83 0.09453808447771195 1103 84-85 0.0689823871246702 1103 86-87 0.0810868900866879 1103 88-89 0.050342492036023145 1103 90-91 0.11056222173497332 1103 92-93 0.1302927605845312 1103 94-95 0.03821129414561142 1103 96-97 0.05895117094721769 1103 98-99 0.07288169311203063 1103 100-101 -0.03026854355800168 1103 102-103 0.013067403047610782 1103 104-105 0.06976370787499775 1103 106-107 0.115180874846466 1103 108-109 0.12762672951274823 1103 110-111 0.12392038377510772 1103 112-113 0.17418111779252854 1103 114-115 0.15522821098851125 1103 116-117 0.13624776362769708 1103 118-119 0.11904948159226336 1103 120-121 0.2077324389012034 1103 122-123 0.1835127734226525 1103 124-125 -0.10158503601495639 1104 1 0.037762187018742566 1104 2 0.05768481644507517 1104 3 0.14563337222181616 1104 4 0.15504935625178717 1104 5 0.0863801773440116 1104 6 0.12532451014421042 1104 7 0.19904140630512757 1104 8 0.17076793268861223 1104 9 0.24994867560626943 1104 10-11 0.2275490788257386 1104 12-13 0.23526211047212797 1104 14-15 0.22192198733051782 1104 16-17 0.17392715322056063 1104 18-19 0.26381941630898353 1104 20-21 0.22468988202880524 1104 22-23 0.2109816546277301 1104 24-25 0.2370769587211683 1104 26-27 0.23006177766284708 1104 28-29 0.25797330702100396 1104 30-31 0.1942569870877051 1104 32-33 0.1771892737555163 1104 34-35 0.22690462929014643 1104 36-37 0.32620526849861164 1104 38-39 0.23056356084376262 1104 40-41 0.14977263969564802 1104 42-43 0.20416817750134442 1104 44-45 0.1682467095375415 1104 46-47 0.030378528111860703 1104 48-49 0.030328969487882773 1104 50-51 0.007887378679079404 1104 52-53 0.1822207008408654 1104 54-55 0.14427545128594232 1104 56-57 0.17396008788694672 1104 58-59 0.16732013551956726 1104 60-61 0.14228207808870508 1104 62-63 0.10579023893000539 1104 64-65 0.049606651059590945 1104 66-67 0.06234042841578713 1104 68-69 0.11197604427533747 1104 70-71 0.16318309587729374 1104 72-73 0.23411218245041 1104 74-75 0.2085626959662008 1104 76-77 0.20778648451088344 1104 78-79 0.21227271180152485 1104 80-81 0.12782368625305907 1104 82-83 0.15941443454145343 1104 84-85 0.15829748995373905 1104 86-87 0.15324779160636837 1104 88-89 0.13204563175192163 1104 90-91 0.12915101031403964 1104 92-93 0.0955423026707507 1104 94-95 0.23427410564372053 1104 96-97 0.10828042306982866 1104 98-99 0.14174303840500357 1104 100-101 0.13179796913451014 1104 102-103 0.05790330736455118 1104 104-105 0.05762255017028295 1104 106-107 0.05721458470137719 1104 108-109 0.13794955762702443 1104 110-111 0.11244539260740538 1104 112-113 0.10768725722915207 1104 114-115 0.09227938279846626 1104 116-117 0.04808344724737523 1104 118-119 0.0054746396705311895 1104 120-121 0.004461009461330434 1104 122-123 -0.06574584236059522 1104 124-125 -0.20923585241862241 1105 1 0.05156742618518706 1105 2 0.07789958178449297 1105 3 0.05395039438862881 1105 4 0.0685323457731144 1105 5 0.01977874122732004 1105 6 0.09119916415832563 1105 7 0.09128734171206077 1105 8 0.1330793979070748 1105 9 0.0806054177997737 1105 10-11 0.1111015894890528 1105 12-13 0.12416014542474585 1105 14-15 0.204356004391812 1105 16-17 0.17954275400790465 1105 18-19 0.14744010778951377 1105 20-21 0.13272592300166508 1105 22-23 0.16192598012040094 1105 24-25 0.14423019574309848 1105 26-27 0.14409074307611291 1105 28-29 0.07328068393134402 1105 30-31 0.17858963875403333 1105 32-33 0.10697256039274805 1105 34-35 0.1271603814643143 1105 36-37 0.06381689870618601 1105 38-39 0.14428772412538393 1105 40-41 0.22026440548564352 1105 42-43 0.11374908568677 1105 44-45 0.15302041909911424 1105 46-47 0.11525888136472418 1105 48-49 0.1017999346233438 1105 50-51 0.08728785755403123 1105 52-53 0.1291546391342564 1105 54-55 -0.04411931606924924 1105 56-57 0.05850683155417613 1105 58-59 -0.08245981195155849 1105 60-61 -0.1468414299795029 1105 62-63 -0.08144661911160966 1105 64-65 -0.052286139475391735 1105 66-67 0.02066135721638318 1105 68-69 -0.033411025846717735 1105 70-71 0.036190924390261614 1105 72-73 0.08743587024494559 1105 74-75 0.12146842476408182 1105 76-77 0.06975330840280236 1105 78-79 0.13825862335787775 1105 80-81 0.15752574878629844 1105 82-83 0.15336730423393163 1105 84-85 0.13675880983272037 1105 86-87 0.04206001824787364 1105 88-89 0.1044100620313202 1105 90-91 0.13420247836138088 1105 92-93 0.14542371674937016 1105 94-95 0.11829001294830022 1105 96-97 0.11280497197537898 1105 98-99 0.07501895092689637 1105 100-101 0.09544790637428235 1105 102-103 0.14439755561387457 1105 104-105 0.08549537643031613 1105 106-107 0.09175309161770429 1105 108-109 0.08891770405740118 1105 110-111 0.0924371812655096 1105 112-113 0.07478286663861411 1105 114-115 0.03739832444585289 1105 116-117 -0.007929687935277485 1105 118-119 -0.05198728494265836 1105 120-121 0.008262672311005304 1105 122-123 0.05460706324327802 1105 124-125 -0.06867109292327811 1106 1 0.11887900539818474 1106 2 0.09961894406866634 1106 3 0.08845941380170785 1106 4 0.04630668123131443 1106 5 0.11390776472295983 1106 6 0.06035028814933696 1106 7 0.12653682817691703 1106 8 0.08921694656834944 1106 9 0.18361733358322851 1106 10-11 0.14357399802514692 1106 12-13 0.1203974858591721 1106 14-15 0.1709419640676515 1106 16-17 0.1943852457865134 1106 18-19 0.1621995720252869 1106 20-21 0.16131261239567607 1106 22-23 0.07325738629054257 1106 24-25 0.14780399653003684 1106 26-27 0.16475601218781577 1106 28-29 0.13937573133087255 1106 30-31 0.0616727442513465 1106 32-33 0.1518669039517846 1106 34-35 0.21363494861606114 1106 36-37 0.287241454847873 1106 38-39 0.13525344344427026 1106 40-41 0.13559901502213023 1106 42-43 0.1979068679543019 1106 44-45 0.11562854074988138 1106 46-47 0.05954041836979229 1106 48-49 0.13134377638058936 1106 50-51 0.16960989231048273 1106 52-53 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68-69 -0.7930206453906976 2216 70-71 -0.8484992586828852 2216 72-73 -0.8327409646953186 2216 74-75 -0.8310603962031564 2216 76-77 -0.7640766773160124 2216 78-79 -0.7138599076626946 2216 80-81 -0.623203954376109 2216 82-83 -0.5869117496408123 2216 84-85 -0.5591434267927511 2216 86-87 -0.632434165841083 2216 88-89 -0.5333705148374293 2216 90-91 -0.5151663618947424 2216 92-93 -0.5250573157335623 2216 94-95 -0.5753924242558419 2216 96-97 -0.6290492839189987 2216 98-99 -0.5762860890775485 2216 100-101 -0.6292218411154593 2216 102-103 -0.741702199423063 2216 104-105 -0.7239320634274726 2216 106-107 -0.5825594121273632 2216 108-109 -0.4906097790291639 2216 110-111 -0.45018195110585424 2216 112-113 -0.4453161511550974 2216 114-115 -0.47297885553994234 2216 116-117 -0.44582706631689817 2216 118-119 -0.3254216863453827 2216 120-121 -0.2763977018105095 2216 122-123 -0.3214913114688862 2216 124-125 -0.3551619132285353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 262.0 3 19.0 4 51.0 5 153.0 6 327.0 7 702.0 8 1217.0 9 2110.0 10 3743.0 11 4814.0 12 6105.0 13 8682.0 14 13314.0 15 10669.0 16 4835.0 17 4488.0 18 4430.0 19 4305.0 20 4498.0 21 4821.0 22 5128.0 23 5786.0 24 7382.0 25 9899.0 26 13129.0 27 17343.0 28 22407.0 29 28807.0 30 36166.0 31 46019.0 32 59057.0 33 76712.0 34 104917.0 35 160306.0 36 317826.0 37 704975.0 38 372965.0 39 1274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2420637298778 19.67190692800956 13.066645143704436 24.019384198408204 2 15.65240961847043 21.428574879822268 38.548773870662714 24.370241631044582 3 18.01600565894698 26.49123544495355 30.594020321379094 24.898738574720376 4 12.32937274689403 17.20470631891587 37.81154527616599 32.654375658024115 5 12.728410144052429 39.17049354526965 34.64123224604698 13.459864064630938 6 31.091177688656202 37.582448064183 17.7083630329193 13.618011214241497 7 27.728018793579373 33.38411503819741 22.034428159832395 16.85343800839082 8 26.697454585162756 34.891089912608116 20.40250419999971 18.008951302229416 9 27.32350458509028 15.133576177147459 20.354476593306188 37.188442644456075 10-11 24.62216913738263 26.829627138593466 28.29700581211349 20.251197911910413 12-13 25.45547710402229 24.565009520965695 28.450293118185115 21.529220256826903 14-15 22.580101978940327 25.449558208831185 26.43890757971302 25.53143223251546 16-17 21.929216777966055 29.039573491660153 26.987287179479747 22.04392255089404 18-19 21.907183992601624 27.195173273844812 29.278527746089544 21.619114987464023 20-21 22.56327732438427 26.65415885230254 29.13719998096287 21.645363842350324 22-23 22.556619668222975 26.779835942720553 28.77757178411929 21.88597260493718 24-25 22.230307352524083 26.9695788114182 29.063128278645156 21.73698555741256 26-27 22.047892008037167 27.16548397048659 28.860154360514205 21.926469660962038 28-29 21.83712803881645 26.839403799118493 29.366898945612714 21.956569216452344 30-31 21.979828844505935 26.58722767285205 29.338505251037798 22.094438231604215 32-33 21.99110329011087 26.836628469230057 28.97583953549443 22.19642870516464 34-35 22.510533232832763 26.718777178639762 28.766645406930675 22.0040441815968 36-37 22.700291789453544 26.34084718958777 28.563935905854294 22.394925115104392 38-39 22.222649026909473 26.624615936178365 28.86193415965942 22.290800877252746 40-41 22.364796599990047 26.270667231659985 29.146286723156333 22.218249445193635 42-43 22.479529078203342 26.24121648033018 28.755828903825442 22.52342553764103 44-45 22.16298225418158 26.62718319709805 28.556550225461642 22.653284323258728 46-47 22.558914750031768 26.875577092281134 28.014662432119934 22.55084572556716 48-49 22.598958366048475 26.84016924739797 27.88897838409032 22.67189400246324 50-51 22.370114072813525 26.656336382651503 28.15934922109755 22.814200323437422 52-53 22.671711981245075 26.78232429457641 27.839463134463287 22.70650058971523 54-55 22.142224528578115 26.822137924270027 28.023601171796297 23.012036375355557 56-57 22.20690288298583 27.019642281215233 28.09714721262247 22.676307623176466 58-59 22.455273687297762 26.39662975691943 28.08310418753379 23.06499236824902 60-61 22.214169303594872 26.763069766138408 28.240570958373013 22.782189971893704 62-63 22.277199497691146 26.741544314647502 28.263884157799197 22.717372029862155 64-65 21.908464406663374 26.560571074335044 28.297440669719364 23.23352384928222 66-67 21.995266816547588 26.82655897659645 27.989851389829067 23.1883228170269 68-69 21.983034259798135 26.603133924401885 28.24346632013322 23.17036549566676 70-71 21.89446819654515 26.6524178221204 28.187079743037625 23.266034238296825 72-73 22.090645298683803 26.78194766863875 27.626612087086706 23.500794945590737 74-75 21.964440244042088 26.982093046965105 27.664312154318406 23.389154554674406 76-77 22.112085085246626 26.735179320868053 27.87974925131979 23.272986342565527 78-79 22.109422279451643 26.82494682052577 27.674344723786838 23.391286176235752 80-81 22.0051284330883 27.138852301706866 27.48623189176078 23.36978737344405 82-83 22.20535135769792 26.849992969802038 27.486068853420615 23.458586819079425 84-85 22.013971011901816 26.677771644136612 27.455973676613233 23.852283667348345 86-87 22.00795016338567 26.502614218973996 27.736208611823393 23.753227005816946 88-89 22.157734449854395 26.54832258510284 27.517958411184924 23.77598455385784 90-91 21.956032996995134 26.736036118306394 27.70760464485904 23.600326239839433 92-93 21.98507182156536 26.522931732670802 27.577533903190066 23.914462542573766 94-95 21.91501142950741 26.284798875941405 27.343822098787086 24.4563675957641 96-97 22.175950151789465 26.23800336579787 27.441616742597635 24.14442973981503 98-99 21.914922401963626 26.29549100326627 27.317117952880693 24.47246864188941 100-101 21.57369652640576 26.293834250641297 27.608191364404394 24.52427785854855 102-103 21.94808476630994 26.106241511217153 27.292508901293605 24.653164821179306 104-105 21.772533978476233 26.012275689390172 27.56152219321527 24.653668138918324 106-107 21.793248201016503 26.03763164722771 27.524870255276156 24.644249896479632 108-109 21.80121419040645 26.30414095026139 26.982215727169788 24.912429132162373 110-111 21.57452715817509 26.327781592328037 27.295764657335543 24.801926592161333 112-113 21.560191781867694 26.328188001505577 27.437364801562396 24.674255415064337 114-115 21.395235796705038 26.63000333874006 27.05092134247307 24.923839522081828 116-117 21.193201241277176 26.470610262400392 27.34914363229398 24.987044864028448 118-119 21.22812968226887 26.814745344970127 26.814552074923064 25.142572897837933 120-121 21.10777987422903 26.82030889504671 27.089221046223127 24.982690184501134 122-123 21.082631819900392 26.983027179946202 26.988680357503082 24.945660642650324 124-125 21.06099940907683 27.07133549119341 26.852747067972594 25.014918031757166 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 93.0 1 102.5 2 243.0 3 374.0 4 266.5 5 222.5 6 488.0 7 1237.5 8 2112.5 9 2307.0 10 1667.5 11 1077.0 12 1018.0 13 1087.5 14 1125.5 15 1244.0 16 1429.0 17 1574.5 18 1727.5 19 1875.5 20 2059.5 21 2323.0 22 2787.5 23 3491.0 24 4433.5 25 5764.0 26 7700.5 27 10021.5 28 12399.0 29 16215.0 30 21995.5 31 26813.5 32 31571.0 33 37423.0 34 43227.0 35 47720.0 36 55589.0 37 64741.5 38 67595.0 39 69048.5 40 68747.0 41 69988.0 42 72797.0 43 74941.5 44 78575.0 45 82092.5 46 80442.0 47 75683.0 48 74809.0 49 74169.0 50 71867.5 51 68673.0 52 66338.5 53 67132.5 54 67857.0 55 62279.0 56 55262.5 57 50246.5 58 47237.0 59 43163.0 60 35377.0 61 29018.0 62 24247.5 63 18985.0 64 14809.5 65 11435.0 66 7943.5 67 6478.5 68 4797.5 69 2727.0 70 1832.0 71 1420.0 72 1175.5 73 866.0 74 761.5 75 695.5 76 398.5 77 130.5 78 43.0 79 22.0 80 14.5 81 8.0 82 4.0 83 2.0 84 1.5 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025849868793796804 2 0.0 3 0.0 4 0.0 5 0.056048313646363174 6 0.014978428646872913 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.6911129117437162E-4 22-23 0.0 24-25 0.0 26-27 3.6238133823079634E-4 28-29 2.415875588205309E-4 30-31 2.174288029384778E-4 32-33 7.247626764615927E-5 34-35 1.4495253529231853E-4 36-37 0.0 38-39 0.0 40-41 9.663502352821236E-5 42-43 0.0 44-45 2.415875588205309E-5 46-47 0.0 48-49 4.831751176410618E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.415875588205309E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.247626764615927E-5 70-71 7.247626764615927E-5 72-73 2.174288029384778E-4 74-75 0.0 76-77 4.831751176410618E-5 78-79 2.415875588205309E-5 80-81 2.415875588205309E-4 82-83 0.0 84-85 2.415875588205309E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.4495253529231853E-4 100-101 0.0 102-103 0.0 104-105 0.0011596202823385483 106-107 9.663502352821236E-5 108-109 0.0016186366440975569 110-111 0.003962035964656707 112-113 0.0 114-115 0.0 116-117 2.415875588205309E-5 118-119 0.0 120-121 3.8654009411284945E-4 122-123 5.073338735231148E-4 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2069643.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.3750378601665 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55425744534772 31.260602622980283 2 11.731507751031046 9.707831547114926 3 4.448818422531311 5.522100919957176 4 2.284847563676744 3.7814261780733793 5 1.292632778787567 2.6741365080813906 6 0.8724364104346476 2.1658253707392787 7 0.5814349864226606 1.6839826203524082 8 0.43209672014140715 1.430241452408359 9 0.3408254105448863 1.2691497838501316 >10 2.037073884524456 16.169531014472206 >50 0.24759663175611324 7.099115646463698 >100 0.16546109461991138 12.747274262077383 >500 0.009225348800739996 2.508871613128074 >1k 0.0017855513807883865 1.9799104603013071 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4849 0.23429161454415087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4235 0.20462466232098966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2720 0.13142363199836882 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.8317511764106175E-5 0.0 22-23 0.0 0.0 0.0 1.2079377941026545E-4 0.0 24-25 0.0 0.0 0.0 5.073338735231148E-4 0.0 26-27 0.0 0.0 0.0 0.001352890329394973 0.0 28-29 0.0 0.0 0.0 0.005194132514641414 0.0 30-31 0.0 0.0 0.0 0.019906814846811748 0.0 32-33 0.0 0.0 0.0 0.05138567376112692 0.0 34-35 0.0 0.0 0.0 0.09607937214292513 0.0 36-37 0.0 0.0 0.0 0.17321827967432063 0.0 38-39 0.0 0.0 0.0 0.2856048120376316 0.0 40-41 0.0 0.0 0.0 0.4112303426243077 0.0 42-43 0.0 0.0 0.0 0.5344158388668964 0.0 44-45 0.0 0.0 0.0 0.6827747587385844 0.0 46-47 0.0 0.0 0.0 0.864617714262798 0.0 48-49 0.0 0.0 0.0 1.0613424634103563 0.0 50-51 0.0 0.0 0.0 1.2822742859517318 0.0 52-53 0.0 0.0 0.0 1.4929869547549988 0.0 54-55 0.0 0.0 0.0 1.7149334450434206 0.0 56-57 0.0 0.0 0.0 1.978698741763676 0.0 58-59 0.0 0.0 0.0 2.2680240022071443 0.0 60-61 0.0 0.0 0.0 2.583561512782639 0.0 62-63 0.0 0.0 0.0 2.9034959169286685 0.0 64-65 0.0 0.0 0.0 3.2225606058629435 0.0 66-67 0.0 0.0 0.0 3.5862706756672527 0.0 68-69 0.0 0.0 0.0 3.9910989479828163 0.0 70-71 0.0 0.0 0.0 4.447433687838917 0.0 72-73 0.0 0.0 0.0 4.902922871239147 0.0 74-75 0.0 0.0 0.0 5.359426722386422 0.0 76-77 0.0 2.4158755882053087E-5 0.0 5.8353058957511035 0.0 78-79 0.0 4.8317511764106175E-5 0.0 6.367281700273912 0.0 80-81 0.0 4.8317511764106175E-5 0.0 6.95726267766953 0.0 82-83 0.0 4.8317511764106175E-5 0.0 7.558235888991483 0.0 84-85 0.0 4.8317511764106175E-5 0.0 8.152807030004691 0.0 86-87 0.0 4.8317511764106175E-5 0.0 8.760158152879505 0.0 88-89 0.0 4.8317511764106175E-5 0.0 9.413676658244924 0.0 90-91 0.0 4.8317511764106175E-5 0.0 10.129452277518393 0.0 92-93 0.0 4.8317511764106175E-5 0.0 10.865762839291607 0.0 94-95 0.0 4.8317511764106175E-5 0.0 11.604851657991258 0.0 96-97 0.0 4.8317511764106175E-5 0.0 12.35302416890256 0.0 98-99 0.0 4.8317511764106175E-5 0.0 13.116730759846021 0.0 100-101 0.0 4.8317511764106175E-5 0.0 13.95356590484446 0.0 102-103 0.0 4.8317511764106175E-5 0.0 14.831205188527683 0.0 104-105 0.0 4.8317511764106175E-5 0.0 15.712154221766749 0.0 106-107 0.0 4.8317511764106175E-5 0.0 16.57776244502071 0.0 108-109 0.0 4.8317511764106175E-5 0.0 17.442935810668796 0.0 110-111 0.0 4.8317511764106175E-5 0.0 18.371525910507273 0.0 112-113 0.0 4.8317511764106175E-5 0.0 19.3421764043364 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3390 0.0 76.78146 1 GTATCAA 6400 0.0 57.79447 1 ATCAACG 8315 0.0 43.89503 3 TCAACGC 8460 0.0 43.07231 4 CAACGCA 8525 0.0 42.7439 5 AACGCAG 8850 0.0 41.35105 6 TATCAAC 9050 0.0 40.330074 2 GTACATG 11095 0.0 38.706387 1 TACATGG 12105 0.0 35.61155 2 ACATGGG 12020 0.0 34.922215 3 ACGCAGA 10595 0.0 34.423203 7 CGCAGAG 10660 0.0 34.15749 8 CATGGGA 8430 0.0 32.27794 4 GCAGAGT 11880 0.0 30.299166 9 CATGGGG 3940 0.0 27.806015 4 GAGTACT 5975 0.0 27.333559 12-13 CATATAG 755 0.0 26.81323 3 ATGGGAG 3685 0.0 26.337055 5 GAGTACA 8540 0.0 26.224344 1 CAGAGTA 11610 0.0 25.904825 10-11 >>END_MODULE