FastQCFastQC Report
Thu 26 May 2016
SRR1364379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631830
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.053.8957141
GTATCAA21700.048.5539631
ATCAACG25650.039.9103623
TCAACGC25800.039.447634
GTACATG66850.038.2893681
CAACGCA26650.038.1894535
AACGCAG27100.037.1149376
TATCAAC29200.035.46592
ACATGGG70800.034.046023
TACATGG72700.033.893042
CATGGGG40650.030.0149694
ACGCAGA35150.028.4368927
CGCAGAG36050.027.3960348
CATGGGA39800.026.1697484
GGACCGA3553.6379788E-1223.4709646
GAGTACA53050.023.3393691
ATGGGAG20700.022.426885
ATGGGGG24500.022.3494245
GCAGAGT43800.021.7335559
TAAGGTG8050.021.4410825
Produced by FastQC (version 0.11.5)