##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364379_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1631830 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.133561093986504 31.0 31.0 34.0 28.0 34.0 2 31.228513386811127 33.0 31.0 34.0 28.0 34.0 3 31.297906644687252 33.0 31.0 34.0 28.0 34.0 4 34.73145915934871 37.0 35.0 37.0 32.0 37.0 5 34.80508263728452 37.0 35.0 37.0 32.0 37.0 6 34.869605902575636 37.0 35.0 37.0 32.0 37.0 7 34.77269078274085 37.0 35.0 37.0 32.0 37.0 8 34.699407413762465 37.0 35.0 37.0 32.0 37.0 9 36.28061501504446 39.0 37.0 39.0 32.0 39.0 10-11 36.18441810727833 39.0 37.0 39.0 31.5 39.0 12-13 36.199036051549484 39.0 37.0 39.0 32.0 39.0 14-15 37.524630323011586 40.0 38.0 41.0 32.0 41.0 16-17 37.53164055079267 40.0 38.0 41.0 32.0 41.0 18-19 37.53213079793851 40.0 38.0 41.0 32.0 41.0 20-21 37.5565202870397 40.0 38.0 41.0 32.0 41.0 22-23 37.522426968495495 40.0 38.0 41.0 31.5 41.0 24-25 37.531545565408166 40.0 38.0 41.0 31.0 41.0 26-27 37.49035377459661 40.0 38.0 41.0 31.0 41.0 28-29 37.4887181875563 40.0 38.0 41.0 31.0 41.0 30-31 37.39335408712917 40.0 38.0 41.0 30.5 41.0 32-33 37.35756512626928 40.0 38.0 41.0 30.5 41.0 34-35 37.343117236476836 40.0 38.0 41.0 30.0 41.0 36-37 37.284923061838555 40.0 38.0 41.0 30.0 41.0 38-39 37.195454183340175 40.0 37.0 41.0 30.0 41.0 40-41 37.13295962201945 40.0 37.0 41.0 30.0 41.0 42-43 37.00208967845916 40.0 37.0 41.0 30.0 41.0 44-45 36.88707861725793 40.0 37.0 41.0 30.0 41.0 46-47 36.77678526562203 40.0 36.5 41.0 30.0 41.0 48-49 36.63595441927161 40.0 36.0 41.0 30.0 41.0 50-51 36.51149844040127 40.0 36.0 41.0 30.0 41.0 52-53 36.34515850303034 40.0 35.5 41.0 29.5 41.0 54-55 36.158108687792236 39.0 35.0 41.0 29.0 41.0 56-57 35.95839548237255 39.0 35.0 41.0 29.0 41.0 58-59 35.733667722740726 39.0 35.0 41.0 28.5 41.0 60-61 35.47766648486669 38.5 35.0 40.5 28.0 41.0 62-63 35.20027729604187 38.0 34.5 40.0 28.0 41.0 64-65 34.85809551240019 37.0 34.0 40.0 28.0 41.0 66-67 34.543006930869026 37.0 34.0 40.0 27.5 41.0 68-69 34.21686327619911 36.0 34.0 39.0 27.0 41.0 70-71 33.85997162694643 36.0 34.0 39.0 26.0 41.0 72-73 33.4936932768732 35.0 33.0 38.5 26.0 40.0 74-75 33.150551528039074 35.0 33.0 37.5 26.0 39.5 76-77 32.916010858974275 35.0 33.0 37.0 26.0 39.0 78-79 32.74937003241759 35.0 33.0 36.5 26.0 39.0 80-81 32.48741167891263 35.0 33.0 36.0 26.0 38.5 82-83 32.206767861848355 35.0 33.0 36.0 26.0 37.0 84-85 31.96801627620524 35.0 33.0 35.5 25.5 37.0 86-87 31.76122328919066 35.0 33.0 35.0 25.0 36.5 88-89 31.51224085842275 35.0 33.0 35.0 24.5 36.0 90-91 31.394006115833143 35.0 33.0 35.0 24.0 36.0 92-93 31.250578798036557 35.0 32.0 35.0 24.0 36.0 94-95 31.153213876445463 35.0 32.0 35.0 24.0 35.0 96-97 31.050316822218 35.0 32.0 35.0 23.0 35.0 98-99 30.917450040751795 35.0 32.0 35.0 22.0 35.0 100-101 30.827755035757402 35.0 32.0 35.0 20.0 35.0 102-103 29.817176115159054 33.5 30.0 34.5 19.0 35.0 104-105 30.51277216376706 34.5 31.5 35.0 19.0 35.0 106-107 30.840540681320974 35.0 32.5 35.0 20.0 35.0 108-109 30.868456579423103 35.0 33.0 35.0 20.0 35.0 110-111 30.797151664082655 35.0 33.0 35.0 19.0 35.0 112-113 30.699014603236854 35.0 32.5 35.0 18.5 35.0 114-115 30.593743527205653 35.0 32.0 35.0 17.5 35.0 116-117 30.459047817480986 35.0 32.0 35.0 12.5 35.0 118-119 30.28555027178076 35.0 32.0 35.0 3.5 35.0 120-121 30.10460740395752 35.0 32.0 35.0 2.0 35.0 122-123 29.91542869048859 35.0 32.0 35.0 2.0 35.0 124-125 29.10132642493397 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11016650541194295 1101 2 0.11862449981994416 1101 3 0.15131853121448913 1101 4 0.17037261248152902 1101 5 0.055121757168052454 1101 6 -0.016455232261861852 1101 7 0.07226117373133434 1101 8 0.005220626434287112 1101 9 0.041875593778108566 1101 10-11 0.17275746078050247 1101 12-13 0.20337235594184477 1101 14-15 0.2722117101126358 1101 16-17 0.198853285862036 1101 18-19 0.12378338314809412 1101 20-21 0.2594687478775768 1101 22-23 0.27024747469423716 1101 24-25 0.214480738145987 1101 26-27 0.2877245088496352 1101 28-29 0.2828288091652382 1101 30-31 0.3107809931395096 1101 32-33 0.28084040104933194 1101 34-35 0.3197244361256679 1101 36-37 0.24590212075924 1101 38-39 0.35305854486047394 1101 40-41 0.23649738365050865 1101 42-43 0.34317602253092616 1101 44-45 0.23612152279212495 1101 46-47 0.23979229082336673 1101 48-49 0.18668064185551714 1101 50-51 0.11568205969177825 1101 52-53 0.12675730786776285 1101 54-55 0.21375546170444437 1101 56-57 0.10731654125510204 1101 58-59 0.12655593253037978 1101 60-61 0.06107524910370188 1101 62-63 0.1468983300571267 1101 64-65 0.1836784150962174 1101 66-67 0.16969343987723562 1101 68-69 0.12394848438222539 1101 70-71 0.04696843432978426 1101 72-73 0.050667959827151776 1101 74-75 0.11664858429796965 1101 76-77 0.2046396672640256 1101 78-79 0.03138945281013861 1101 80-81 0.11399094949145905 1101 82-83 0.08737086664726945 1101 84-85 0.18282610588806492 1101 86-87 0.13039222555597263 1101 88-89 0.11180177307361916 1101 90-91 0.02192806855052254 1101 92-93 0.10348430478885717 1101 94-95 0.05611731673065634 1101 96-97 0.007735661361344626 1101 98-99 -0.0242860525356825 1101 100-101 -0.08468268814701574 1101 102-103 -0.14993789910459654 1101 104-105 -0.09734102769344233 1101 106-107 0.00926419532616407 1101 108-109 -0.0797677337000735 1101 110-111 -0.037857035394655014 1101 112-113 0.0013939913439564577 1101 114-115 -0.06895672231197025 1101 116-117 -0.12002348311574451 1101 118-119 -0.07040485230677618 1101 120-121 -0.34777513494472956 1101 122-123 -0.23044970756570038 1101 124-125 -0.3393277810986852 1102 1 0.2255909776207261 1102 2 0.26982746556912574 1102 3 0.3237627033530508 1102 4 0.3975968830214285 1102 5 0.3582677293126082 1102 6 0.38442388530536675 1102 7 0.28743566399548826 1102 8 0.268028043306515 1102 9 0.3781135299853915 1102 10-11 0.5147821215737736 1102 12-13 0.4003886524564777 1102 14-15 0.5321730853937297 1102 16-17 0.5609011273672664 1102 18-19 0.4881136445255976 1102 20-21 0.47144619639311003 1102 22-23 0.46525011454620113 1102 24-25 0.4541061566585469 1102 26-27 0.44155731111912644 1102 28-29 0.3173811911607345 1102 30-31 0.4467595567696776 1102 32-33 0.37397369859657914 1102 34-35 0.2549568212011337 1102 36-37 0.13567695323936846 1102 38-39 0.3140048155573112 1102 40-41 0.2723468123012722 1102 42-43 0.20069596200453788 1102 44-45 0.19098097295680816 1102 46-47 0.23169006781624546 1102 48-49 0.2908655744662525 1102 50-51 0.1929348972340108 1102 52-53 0.1311571224073731 1102 54-55 0.1517400739310517 1102 56-57 0.1953861561638277 1102 58-59 0.16240536118115045 1102 60-61 0.17337232828621296 1102 62-63 0.2322712162689342 1102 64-65 0.15870934813364812 1102 66-67 0.20935011761847733 1102 68-69 0.1346982776872423 1102 70-71 0.10826748775035355 1102 72-73 0.22002323869494944 1102 74-75 0.19399505251049476 1102 76-77 0.1996335045465969 1102 78-79 0.28797047605093695 1102 80-81 0.17781196660905607 1102 82-83 0.19729005759006668 1102 84-85 0.19855348571966758 1102 86-87 0.14999540058211025 1102 88-89 0.19370582212690124 1102 90-91 0.18907379223842113 1102 92-93 0.2320119252852777 1102 94-95 0.08129817829086505 1102 96-97 0.0923694257755514 1102 98-99 0.18289675220550095 1102 100-101 0.16612484192735977 1102 102-103 -0.06938409803935741 1102 104-105 -0.02406450738036625 1102 106-107 0.07671622637662878 1102 108-109 0.1696558266433783 1102 110-111 0.18553067478506335 1102 112-113 0.17218671693001042 1102 114-115 0.1628152055040175 1102 116-117 0.012092149200011448 1102 118-119 0.027201859551311713 1102 120-121 0.025992803965806388 1102 122-123 0.1024393012227165 1102 124-125 0.08153886585561665 1103 1 0.2892120455224365 1103 2 0.3119141568927333 1103 3 0.3211739588157805 1103 4 0.309401028435758 1103 5 0.28254285291709635 1103 6 0.21766650718525682 1103 7 0.19399746209647617 1103 8 0.21414778206473528 1103 9 0.33327072383603706 1103 10-11 0.40350267931138717 1103 12-13 0.31378786014102644 1103 14-15 0.33327439630485145 1103 16-17 0.2401097559632248 1103 18-19 0.22605330005989543 1103 20-21 0.19657472830705558 1103 22-23 0.3599960026689857 1103 24-25 0.40825498505751767 1103 26-27 0.4769096453941586 1103 28-29 0.39949613545380913 1103 30-31 0.4674541092361153 1103 32-33 0.5763074421582743 1103 34-35 0.5552250640671375 1103 36-37 0.4258193837334048 1103 38-39 0.3661421917929317 1103 40-41 0.3677077006573697 1103 42-43 0.31019585633965363 1103 44-45 0.3604626673494735 1103 46-47 0.3828132773910511 1103 48-49 0.3712342106794466 1103 50-51 0.2813501888609835 1103 52-53 0.3176537451848276 1103 54-55 0.3592297772352282 1103 56-57 0.28142439333851144 1103 58-59 0.24858997979758612 1103 60-61 0.13078325585451012 1103 62-63 0.1832739696813377 1103 64-65 0.16966516580057345 1103 66-67 0.16896163118096297 1103 68-69 0.03903507787359928 1103 70-71 0.08730728674746757 1103 72-73 0.057119340768835514 1103 74-75 0.08132322997416708 1103 76-77 0.14761080128042892 1103 78-79 0.1787056928314925 1103 80-81 0.08171295546299007 1103 82-83 0.06613345683198446 1103 84-85 -0.04962827661200109 1103 86-87 0.04686467537872119 1103 88-89 0.1343417967939935 1103 90-91 -0.06634587930144065 1103 92-93 -0.1598285839816107 1103 94-95 -0.11799434443699042 1103 96-97 -0.1641956242454654 1103 98-99 -0.1518851718754064 1103 100-101 -0.1515692783309852 1103 102-103 -0.2967803262084807 1103 104-105 -0.1500794712570297 1103 106-107 -0.15550842742900883 1103 108-109 -0.0946840146737351 1103 110-111 -0.06310095149743944 1103 112-113 -0.20631119148186983 1103 114-115 -0.2094573028721527 1103 116-117 -0.3026705588260299 1103 118-119 -0.4471500399933639 1103 120-121 -0.5127287356534787 1103 122-123 -0.5588199899451922 1103 124-125 -0.5692708622316225 1104 1 0.36745736524890305 1104 2 0.3762592469079742 1104 3 0.33041483152780415 1104 4 0.4731918037667455 1104 5 0.40515558775155114 1104 6 0.3754354382390588 1104 7 0.4020306828511906 1104 8 0.4642460103546924 1104 9 0.5614065891822619 1104 10-11 0.4209769890392181 1104 12-13 0.42198751297948434 1104 14-15 0.3373118173118357 1104 16-17 0.4356401834336836 1104 18-19 0.37156567047003364 1104 20-21 0.48683826607172875 1104 22-23 0.4701160053378288 1104 24-25 0.5061840891201896 1104 26-27 0.525619227497657 1104 28-29 0.5699600801113078 1104 30-31 0.5663533169495025 1104 32-33 0.5620132402220861 1104 34-35 0.455060273534329 1104 36-37 0.4505051326354135 1104 38-39 0.3414315548605771 1104 40-41 0.4828339041524927 1104 42-43 0.34837277104995934 1104 44-45 0.2547147502657978 1104 46-47 0.24281651813031857 1104 48-49 0.33191377040489556 1104 50-51 0.17283414031766853 1104 52-53 0.029863654009915308 1104 54-55 0.14810117389853872 1104 56-57 0.013150489444434754 1104 58-59 0.18534075741615652 1104 60-61 0.02184887658846435 1104 62-63 0.10239157780394947 1104 64-65 0.14970946649457773 1104 66-67 0.11014036006982764 1104 68-69 0.015556663209359556 1104 70-71 -0.043011735438888365 1104 72-73 -0.10727351308335642 1104 74-75 -0.11883478254186741 1104 76-77 0.025810104627396413 1104 78-79 0.11538445402184294 1104 80-81 0.057827859792340064 1104 82-83 0.09778308843618078 1104 84-85 0.15019030553279222 1104 86-87 0.0977133042600613 1104 88-89 0.22177106448223682 1104 90-91 0.1409724724966921 1104 92-93 0.08174522915058446 1104 94-95 0.11650967948093438 1104 96-97 0.08090037471241729 1104 98-99 -0.04408981714684046 1104 100-101 -0.18510298678518566 1104 102-103 -0.17425973003954098 1104 104-105 -0.09580370764460255 1104 106-107 -0.12178673491585812 1104 108-109 -0.12795083871747792 1104 110-111 -0.12192063401089825 1104 112-113 -0.15005656469111983 1104 114-115 -0.12345141870633825 1104 116-117 -0.12728778621472614 1104 118-119 -0.0888839308185787 1104 120-121 -0.02616403577664883 1104 122-123 -0.08418671771991981 1104 124-125 -0.2150744755500007 1105 1 0.12579130137015682 1105 2 0.08226804656328568 1105 3 2.741775186336781E-4 1105 4 0.14002829937792427 1105 5 0.24054573830176906 1105 6 0.18482970452036085 1105 7 0.2097675444332623 1105 8 0.1978892290954235 1105 9 0.11676139015217757 1105 10-11 0.1831312901669051 1105 12-13 0.2625671433604211 1105 14-15 0.312777562708888 1105 16-17 0.3113280168671295 1105 18-19 0.2074270439249375 1105 20-21 0.27821153227021966 1105 22-23 0.22909885575941047 1105 24-25 0.2559835966162538 1105 26-27 0.291767915883554 1105 28-29 0.2889419241391451 1105 30-31 0.2789387948974209 1105 32-33 0.2675282779461341 1105 34-35 0.1840955717466244 1105 36-37 0.14218644966936722 1105 38-39 0.23630691404355275 1105 40-41 0.24187755752952 1105 42-43 0.09442896556071645 1105 44-45 0.03665045428540026 1105 46-47 0.0441297571352095 1105 48-49 0.019174817410828382 1105 50-51 0.08772730009002316 1105 52-53 0.07538908065271244 1105 54-55 0.11970173831770126 1105 56-57 0.08653651168364007 1105 58-59 0.10806622892734907 1105 60-61 -0.011730079718319075 1105 62-63 -0.059068049557801316 1105 64-65 -0.06306864150614899 1105 66-67 -0.14233264289379122 1105 68-69 -0.13959046421791044 1105 70-71 -0.1321247566014634 1105 72-73 -0.07550449302780748 1105 74-75 -0.055590273939820634 1105 76-77 -0.06070542476192031 1105 78-79 -0.008981439294124982 1105 80-81 -0.052280002553956706 1105 82-83 -0.046536919614972305 1105 84-85 0.04535762373457786 1105 86-87 0.003554424040835613 1105 88-89 0.06250794388586556 1105 90-91 0.06896237920609138 1105 92-93 0.09597207239741934 1105 94-95 0.012436510462347172 1105 96-97 0.042659246964358744 1105 98-99 0.013584931071267903 1105 100-101 -0.09749536293323402 1105 102-103 -0.21194655498259252 1105 104-105 -0.06038939316123404 1105 106-107 -0.09929697968968654 1105 108-109 -0.008786480990629286 1105 110-111 -0.008598339558957946 1105 112-113 -0.055619353701718666 1105 114-115 -0.12486587366549884 1105 116-117 -0.11845248779277284 1105 118-119 -0.14425524293587344 1105 120-121 -0.22948230465360808 1105 122-123 -0.29614477206727585 1105 124-125 -0.43561518155618373 1106 1 0.2299149939976779 1106 2 0.31045188894071174 1106 3 0.2707775297642918 1106 4 0.38019299995887934 1106 5 0.26698985335836767 1106 6 0.18809427439529713 1106 7 0.29240092860688804 1106 8 0.2583946778996733 1106 9 0.38486294218837713 1106 10-11 0.5198950529750164 1106 12-13 0.42532953166538334 1106 14-15 0.6070540410033445 1106 16-17 0.5503068879194544 1106 18-19 0.430508372247175 1106 20-21 0.44364241169744645 1106 22-23 0.42720615792813277 1106 24-25 0.46506885594423153 1106 26-27 0.44759825550107024 1106 28-29 0.5026482717159197 1106 30-31 0.5243970977773671 1106 32-33 0.5091057825884491 1106 34-35 0.4324107063185352 1106 36-37 0.4605606126071038 1106 38-39 0.4157069685640664 1106 40-41 0.3465056565104021 1106 42-43 0.34076469236797635 1106 44-45 0.3284379561650539 1106 46-47 0.31239225626302414 1106 48-49 0.2663207750243686 1106 50-51 0.24071421333462695 1106 52-53 0.21146780667780263 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44-45 -0.6585005290785162 2216 46-47 -0.61795496844762 2216 48-49 -0.8365500238653425 2216 50-51 -0.5936990576671377 2216 52-53 -0.5717360903317683 2216 54-55 -0.4303652521485333 2216 56-57 -0.5931225386027847 2216 58-59 -0.7114973507122784 2216 60-61 -0.6852005256222284 2216 62-63 -0.7666587267302347 2216 64-65 -0.6175643886825029 2216 66-67 -0.7512007182067322 2216 68-69 -0.703142111134575 2216 70-71 -0.7282455876082281 2216 72-73 -0.6075216520411715 2216 74-75 -0.62142144319305 2216 76-77 -0.6200128895826964 2216 78-79 -0.7316754752961501 2216 80-81 -0.6817691023852674 2216 82-83 -0.6812096929759548 2216 84-85 -0.6750879108744492 2216 86-87 -0.7337871844984889 2216 88-89 -0.6759129719114796 2216 90-91 -0.7688590937864852 2216 92-93 -0.7127242798350544 2216 94-95 -0.6307081564465804 2216 96-97 -0.6917178672606639 2216 98-99 -0.715234264097738 2216 100-101 -0.5238032650015931 2216 102-103 -0.5995135085191272 2216 104-105 -0.8536670808305544 2216 106-107 -0.5870040444880047 2216 108-109 -0.5964954661330708 2216 110-111 -0.5758927371765488 2216 112-113 -0.6046433757295446 2216 114-115 -0.5169772477160386 2216 116-117 -0.6376306691382538 2216 118-119 -0.5879327314802829 2216 120-121 -0.3985867743284217 2216 122-123 -0.45722604398504885 2216 124-125 -0.33133133696815165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25049.0 3 4825.0 4 3095.0 5 2650.0 6 2892.0 7 3350.0 8 3709.0 9 3926.0 10 4443.0 11 5014.0 12 5252.0 13 5484.0 14 5362.0 15 4985.0 16 4750.0 17 4338.0 18 4181.0 19 4020.0 20 4111.0 21 4365.0 22 5302.0 23 6990.0 24 10166.0 25 14618.0 26 17562.0 27 18292.0 28 20791.0 29 25686.0 30 30184.0 31 37333.0 32 47978.0 33 63658.0 34 90146.0 35 137005.0 36 268932.0 37 511249.0 38 219483.0 39 654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61088545897644 18.119645019389054 12.59175697009641 24.677712551538097 2 15.70846262228953 19.855535487635144 39.19313885496001 25.24286303511532 3 18.6535187652363 24.28339606592499 29.52002519925922 27.54305996957949 4 12.61300792281142 15.954279592877313 36.971730859742706 34.46098162456856 5 13.514114860690224 38.30749163945198 34.46022978022301 13.718163719634784 6 32.982567551580466 36.1511674450451 17.28919573147446 13.577069271899978 7 29.171211805512996 32.40308129772396 20.84649891529495 17.57920798146809 8 26.7106254940772 35.20740518313795 19.892513313274055 18.189456009510796 9 26.86401156983264 15.888971277645341 19.19207270365173 38.05494444887029 10-11 25.13115651211003 26.445142229976305 27.742367613273206 20.68133364464046 12-13 25.530820301823614 23.852963634335776 27.62499279942444 22.991223264416167 14-15 22.520572021070848 25.456788337986602 24.835123554688362 27.18751608625419 16-17 23.116246590864176 28.849630950801508 25.476758127462535 22.557364330871778 18-19 23.195895399483092 27.712304149795248 26.661978671152436 22.429821779569224 20-21 23.562425964975496 26.99688509028826 26.705892590461378 22.734796354274867 22-23 23.64490285290222 26.793822516384218 26.50025630741447 23.061018323299095 24-25 23.339287793458876 27.09485669463118 26.476226077471303 23.089629434438635 26-27 23.100452590175628 27.455778927884317 26.299959646519444 23.14380883542061 28-29 23.151624714659967 27.414244787278047 26.368973388690577 23.06515710937141 30-31 23.064848380299534 27.07531638568858 26.478991830948022 23.38084340306386 32-33 22.912871718522062 27.2981950626582 26.391421407689958 23.397511811129778 34-35 23.607803196250075 27.155072699567995 26.02041832470952 23.21670577947241 36-37 23.557323443402122 26.842994482261613 26.14301262142824 23.456669452908027 38-39 23.141984589679616 26.93800236973432 26.364228838998706 23.55578420158736 40-41 23.398700901169764 26.76487377464536 26.493980507083027 23.34244481710185 42-43 23.207301293248857 26.731310214573234 26.46890390211423 23.592484590063677 44-45 23.114172877304075 26.992840161280984 26.460762447600377 23.432224513814564 46-47 23.324625313068957 27.213145495024293 26.03336501557455 23.4288641763322 48-49 23.22858385983834 27.07616602219594 26.117886054307128 23.577364063658592 50-51 23.171071741541706 26.963593021331878 26.406641623208298 23.458693613918115 52-53 23.43030516958428 26.830079898812 26.224618737184507 23.514996194419215 54-55 22.998452917869212 26.827948472267888 26.465185443026535 23.70841316683637 56-57 23.05928356966871 27.157482734837313 26.47055308882409 23.31268060666989 58-59 23.198696663186894 26.74739085978987 26.262316450153712 23.791596026869524 60-61 22.92753909170464 26.79748915923726 26.76366156719802 23.511310181860075 62-63 22.95918007787042 26.90549502133669 26.665424694672524 23.46990020612037 64-65 22.896951706032244 26.85350760326952 26.387518553154248 23.862022137543985 66-67 22.75198356644235 27.145735676863897 26.316152331529373 23.786128425164378 68-69 22.79696392244894 26.72795569736419 26.694924212966665 23.780156167220206 70-71 22.850959493760868 26.68204030155693 26.54964033538465 23.917359869297545 72-73 22.850422788282287 26.746491267850075 26.38863184265584 24.0144541012118 74-75 22.74555429042075 27.00119745213562 26.45275334324469 23.80049491419894 76-77 23.01032845896745 26.68258771591161 26.49797585736505 23.809107967755892 78-79 22.88968385895835 26.674245267842778 26.68294738132263 23.75312349187624 80-81 22.83572920129734 26.932671079986704 26.555235697947516 23.676364020768446 82-83 22.799775587512443 26.89634526921379 26.53545910339796 23.768420039875807 84-85 22.81866958937554 26.69458846398146 26.534950709928395 23.9517912367146 86-87 22.95143852101747 26.669822695524413 26.553785013152677 23.824953770305445 88-89 22.90077365076875 26.647470436539066 26.502502843583308 23.94925306910888 90-91 22.802248897933996 26.79916277607569 26.63839151524845 23.76019681074186 92-93 22.906817590591583 26.293168559803483 26.828921529425354 23.97109232017958 94-95 22.697907659536135 26.277952873161013 26.855256957156136 24.168882510146712 96-97 22.87023160856868 26.286955138919488 26.768593352295728 24.074219900216107 98-99 22.832275717529253 26.21154971611365 26.586101978471827 24.370072587885268 100-101 22.501971752613837 26.22777220450828 26.920597353714953 24.34965868916293 102-103 22.59011521019733 25.957868611349433 26.990470645912488 24.46154553254075 104-105 22.546684299416846 26.109686655080367 26.871845531225414 24.47178351427738 106-107 22.540596177694404 26.184408211868337 26.739316355863153 24.53567925457411 108-109 22.624651649484694 26.135350740517744 26.518302669295668 24.721694940701894 110-111 22.430581217417096 26.44467854190375 26.801064346773103 24.323675893906053 112-113 22.428587537106537 26.35648748489387 27.01744957065501 24.197475407344584 114-115 22.388420995219334 26.474477886432062 26.701377206867832 24.43572391148077 116-117 22.244164636950746 26.412454003915315 26.863061662407045 24.48031969672689 118-119 22.136678460567886 26.723578217721016 26.415068890402555 24.724674431308546 120-121 22.134511624429212 26.806439088309624 26.64613911806517 24.41291016919599 122-123 22.204742649897717 26.829022409124946 26.56694117834516 24.399293762632173 124-125 22.133086486483176 26.84036644623267 26.571519094780488 24.45502797250366 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 509.0 1 449.0 2 359.0 3 285.0 4 224.5 5 193.5 6 174.5 7 161.5 8 152.5 9 147.5 10 150.5 11 152.0 12 153.5 13 171.5 14 182.0 15 203.5 16 240.0 17 282.0 18 347.0 19 472.0 20 611.5 21 851.0 22 1251.5 23 1781.0 24 2571.0 25 3765.5 26 5176.5 27 7007.5 28 9119.0 29 11483.5 30 14827.5 31 18441.0 32 22314.0 33 25061.0 34 27608.0 35 30526.5 36 33668.5 37 37696.5 38 40872.0 39 43201.5 40 45203.0 41 48828.5 42 53077.0 43 56598.5 44 59838.5 45 63153.0 46 63765.0 47 64082.5 48 65212.5 49 63945.0 50 63406.0 51 62445.0 52 61498.5 53 62192.5 54 63109.5 55 59799.5 56 53289.5 57 48586.0 58 45221.0 59 39817.5 60 32423.0 61 26255.5 62 21540.0 63 16932.5 64 12774.0 65 9808.5 66 7135.5 67 5752.0 68 4365.0 69 2620.0 70 1809.5 71 1310.5 72 959.5 73 734.5 74 569.0 75 442.5 76 289.0 77 154.5 78 87.0 79 58.5 80 35.0 81 23.5 82 15.0 83 13.0 84 12.0 85 8.5 86 6.5 87 5.0 88 2.5 89 2.5 90 3.5 91 4.0 92 4.0 93 3.5 94 3.5 95 2.5 96 1.5 97 1.0 98 0.0 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04626707438887629 2 0.009130853091314658 3 0.00220611215629079 4 0.005270156817805777 5 0.021938559776447302 6 0.02739255927394398 7 0.003064044661514986 8 0.0 9 0.0 10-11 6.128089323029973E-5 12-13 9.804942916847956E-4 14-15 1.2256178646059945E-4 16-17 9.192133984544958E-5 18-19 1.532022330757493E-4 20-21 6.128089323029973E-5 22-23 3.064044661514986E-5 24-25 0.0 26-27 3.064044661514986E-5 28-29 3.064044661514986E-4 30-31 3.064044661514986E-5 32-33 3.064044661514986E-5 34-35 7.047302721484469E-4 36-37 1.2256178646059945E-4 38-39 9.192133984544958E-5 40-41 3.9832580599694816E-4 42-43 5.821684856878473E-4 44-45 0.0011949774179908447 46-47 6.128089323029973E-5 48-49 0.0 50-51 0.0 52-53 9.804942916847956E-4 54-55 0.0029721233216695366 56-57 2.1448312630604902E-4 58-59 0.001133696524760545 60-61 0.0015933032239877926 62-63 0.0010111347382999456 64-65 0.0019609885833695912 66-67 0.002880201981824087 68-69 0.0032785277878210357 70-71 0.0019609885833695912 72-73 0.002696359302133188 74-75 0.0019609885833695912 76-77 0.0017158650104483922 78-79 0.0026044379622877385 80-81 0.0016852245638332426 82-83 8.272920586090463E-4 84-85 6.434493789181471E-4 86-87 0.0019916290299847412 88-89 0.005270156817805777 90-91 5.208875924575477E-4 92-93 8.885729518393461E-4 94-95 0.0012868987578362942 96-97 2.1448312630604902E-4 98-99 9.192133984544958E-4 100-101 0.002022269476599891 102-103 0.0018384267969089917 104-105 0.001103056078145395 106-107 0.007261785847790518 108-109 0.0019916290299847412 110-111 0.0018997076901392915 112-113 0.0025737975156725885 114-115 0.003002763768284686 116-117 0.0020529099232150408 118-119 0.04663475974825809 120-121 0.012348099985905395 122-123 0.005147595031345177 124-125 0.003002763768284686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1631830.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.8346683262996 #Duplication Level Percentage of deduplicated Percentage of total 1 83.68645593920373 60.115888057961854 2 10.089627362493589 14.49570070241368 3 2.947869975099684 6.352777857910286 4 1.2168175868684243 3.4963875106520605 5 0.6229741118523644 2.2375569350392825 6 0.3629645710852701 1.5644063746864785 7 0.22798428964208142 1.146402308103216 8 0.15317081281954267 0.8802379628929253 9 0.1166882561135918 0.7544035957944752 >10 0.5412326922259392 6.819174220601731 >50 0.025745262054846067 1.2118529314044404 >100 0.008303078977970444 0.835744212815558 >500 1.6606156295380798E-4 0.08946732972400288 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.128089323029973E-5 0.0 0.0 0.0 0.0 3 6.128089323029973E-5 0.0 0.0 0.0 0.0 4 6.128089323029973E-5 0.0 0.0 0.0 0.0 5 6.128089323029973E-5 0.0 0.0 0.0 0.0 6 1.2256178646059945E-4 0.0 0.0 0.0 0.0 7 1.2256178646059945E-4 0.0 0.0 0.0 0.0 8 1.2256178646059945E-4 0.0 0.0 0.0 0.0 9 1.2256178646059945E-4 0.0 0.0 0.0 0.0 10-11 1.2256178646059945E-4 0.0 0.0 0.0 0.0 12-13 1.2256178646059945E-4 0.0 0.0 0.0 0.0 14-15 1.2256178646059945E-4 0.0 0.0 0.0 0.0 16-17 1.2256178646059945E-4 0.0 0.0 3.064044661514986E-5 0.0 18-19 1.532022330757493E-4 0.0 0.0 6.128089323029973E-5 0.0 20-21 1.8384267969089916E-4 3.064044661514986E-5 0.0 9.19213398454496E-5 0.0 22-23 1.8384267969089916E-4 6.128089323029973E-5 0.0 2.757640195363488E-4 0.0 24-25 1.8384267969089916E-4 6.128089323029973E-5 0.0 6.128089323029972E-4 0.0 26-27 1.8384267969089916E-4 6.128089323029973E-5 0.0 0.001103056078145395 0.0 28-29 2.451235729211989E-4 6.128089323029973E-5 0.0 0.0032478873412058852 0.0 30-31 3.064044661514986E-4 6.128089323029973E-5 0.0 0.013726920083587137 0.0 32-33 3.064044661514986E-4 6.128089323029973E-5 0.0 0.03373513172328 0.0 34-35 3.370449127666485E-4 6.128089323029973E-5 0.0 0.06474326369781165 0.0 36-37 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.11934453956600871 0.0 38-39 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.19490388091896826 0.0 40-41 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.27616234534234574 0.0 42-43 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.35582750654173534 0.0 44-45 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.4530496436516059 0.0 46-47 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.574110048228063 0.0 48-49 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.7050366766145983 0.0 50-51 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.8460746523841332 0.0 52-53 3.6768535938179833E-4 6.128089323029973E-5 0.0 0.9813828646366349 0.0 54-55 3.6768535938179833E-4 6.128089323029973E-5 0.0 1.128426367942739 0.0 56-57 3.6768535938179833E-4 6.128089323029973E-5 0.0 1.299920947647733 0.0 58-59 3.6768535938179833E-4 6.128089323029973E-5 0.0 1.4884209752241349 0.0 60-61 3.6768535938179833E-4 6.128089323029973E-5 0.0 1.6934668439727179 0.0 62-63 3.6768535938179833E-4 6.128089323029973E-5 0.0 1.897807982449152 0.0 64-65 3.6768535938179833E-4 6.128089323029973E-5 0.0 2.1086142551613833 0.0 66-67 3.6768535938179833E-4 6.128089323029973E-5 0.0 2.3509189069939884 0.0 68-69 3.6768535938179833E-4 6.128089323029973E-5 0.0 2.6263765220641853 0.0 70-71 3.6768535938179833E-4 6.128089323029973E-5 0.0 2.9261320113001967 0.0 72-73 3.6768535938179833E-4 6.128089323029973E-5 0.0 3.2366422972981255 0.0 74-75 3.6768535938179833E-4 6.128089323029973E-5 0.0 3.5412696175459457 0.0 76-77 3.6768535938179833E-4 6.128089323029973E-5 0.0 3.866119632559764 0.0 78-79 3.6768535938179833E-4 6.128089323029973E-5 0.0 4.237543126428611 0.0 80-81 3.6768535938179833E-4 6.128089323029973E-5 0.0 4.643283920506425 0.0 82-83 4.902471458423978E-4 6.128089323029973E-5 0.0 5.058645814821396 0.0 84-85 4.902471458423978E-4 6.128089323029973E-5 0.0 5.474743079855132 0.0 86-87 4.902471458423978E-4 6.128089323029973E-5 0.0 5.898194052076503 0.0 88-89 4.902471458423978E-4 6.128089323029973E-5 0.0 6.351243695728109 0.0 90-91 4.902471458423978E-4 6.128089323029973E-5 0.0 6.859568705073445 0.0 92-93 4.902471458423978E-4 6.128089323029973E-5 0.0 7.377790578675475 0.0 94-95 4.902471458423978E-4 6.128089323029973E-5 0.0 7.901987339367459 0.0 96-97 4.902471458423978E-4 6.128089323029973E-5 0.0 8.442944424357929 0.0 98-99 5.821684856878475E-4 6.128089323029973E-5 0.0 8.994166058964474 0.0 100-101 6.434493789181471E-4 6.128089323029973E-5 0.0 9.60005025033245 0.0 102-103 6.74089825533297E-4 6.128089323029973E-5 0.0 10.245552539173811 0.0 104-105 6.74089825533297E-4 6.128089323029973E-5 0.0 10.880054907680334 0.0 106-107 6.74089825533297E-4 6.128089323029973E-5 0.0 11.505426423095543 0.0 108-109 6.74089825533297E-4 6.128089323029973E-5 0.0 12.162786564776969 0.0 110-111 6.74089825533297E-4 6.128089323029973E-5 0.0 12.85700716373642 0.0 112-113 6.74089825533297E-4 6.128089323029973E-5 0.0 13.587904377294205 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 53.895714 1 GTATCAA 2170 0.0 48.553963 1 ATCAACG 2565 0.0 39.910362 3 TCAACGC 2580 0.0 39.44763 4 GTACATG 6685 0.0 38.289368 1 CAACGCA 2665 0.0 38.189453 5 AACGCAG 2710 0.0 37.114937 6 TATCAAC 2920 0.0 35.4659 2 ACATGGG 7080 0.0 34.04602 3 TACATGG 7270 0.0 33.89304 2 CATGGGG 4065 0.0 30.014969 4 ACGCAGA 3515 0.0 28.436892 7 CGCAGAG 3605 0.0 27.396034 8 CATGGGA 3980 0.0 26.169748 4 GGACCGA 355 3.6379788E-12 23.470964 6 GAGTACA 5305 0.0 23.339369 1 ATGGGAG 2070 0.0 22.42688 5 ATGGGGG 2450 0.0 22.349424 5 GCAGAGT 4380 0.0 21.733555 9 TAAGGTG 805 0.0 21.441082 5 >>END_MODULE