FastQCFastQC Report
Thu 26 May 2016
SRR1364379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631830
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.1986113749594014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.17507951195896632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.072.78151
GTATCAA45350.055.6748921
ATCAACG57700.042.9261323
TATCAAC58550.042.5063322
TCAACGC58450.042.3753244
CAACGCA59400.041.6976055
AACGCAG60350.041.1159256
GTACATG66000.036.992251
ACGCAGA71600.034.7345247
CGCAGAG72400.034.432898
TACATGG70950.033.734782
ACATGGG69800.033.5228843
GCAGAGT81400.030.2603539
GAGTACT48800.027.73701312-13
GTGGTAT13550.027.6867561
CATGGGG41700.027.5565914
CATGGGA37600.026.444354
CAGAGTA80500.026.27494210-11
TAAGGTG7950.025.463385
GAGTACA53100.025.4567051
Produced by FastQC (version 0.11.5)