##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364379_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1631830 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30336738508301 33.0 31.0 34.0 30.0 34.0 2 32.308058437459785 33.0 31.0 34.0 30.0 34.0 3 32.427721637670594 34.0 31.0 34.0 30.0 34.0 4 36.034945429364576 37.0 35.0 37.0 35.0 37.0 5 35.96729867694552 37.0 35.0 37.0 35.0 37.0 6 36.02654627013843 37.0 35.0 37.0 35.0 37.0 7 35.97948009290183 37.0 35.0 37.0 35.0 37.0 8 35.98324396536404 37.0 35.0 37.0 35.0 37.0 9 37.73775270708346 39.0 38.0 39.0 35.0 39.0 10-11 37.69616473529718 39.0 37.5 39.0 35.0 39.0 12-13 37.65996795009284 39.0 37.0 39.0 35.0 39.0 14-15 39.05465979912123 41.0 38.0 41.0 35.5 41.0 16-17 39.06085192697769 41.0 38.0 41.0 35.5 41.0 18-19 39.11614782177065 41.0 38.5 41.0 36.0 41.0 20-21 39.12239939209354 41.0 39.0 41.0 35.0 41.0 22-23 39.090065447993965 41.0 39.0 41.0 35.0 41.0 24-25 39.058712610995016 41.0 39.0 41.0 35.0 41.0 26-27 38.96886838702561 41.0 38.5 41.0 35.0 41.0 28-29 38.88161297439071 40.5 38.0 41.0 35.0 41.0 30-31 38.77735732276034 40.0 38.0 41.0 35.0 41.0 32-33 38.66394446725455 40.0 38.0 41.0 34.0 41.0 34-35 38.5786077593867 40.0 38.0 41.0 34.0 41.0 36-37 38.4714728249879 40.0 38.0 41.0 34.0 41.0 38-39 38.38912264145162 40.0 38.0 41.0 34.0 41.0 40-41 38.28004663475975 40.0 38.0 41.0 34.0 41.0 42-43 38.13531771079096 40.0 38.0 41.0 33.0 41.0 44-45 37.977224956030966 40.0 38.0 41.0 33.0 41.0 46-47 37.75330150812279 40.0 37.5 41.0 33.0 41.0 48-49 37.55407763063555 40.0 37.0 41.0 32.5 41.0 50-51 37.39319567602017 40.0 37.0 41.0 32.0 41.0 52-53 37.23399159226145 40.0 36.5 41.0 32.0 41.0 54-55 37.05359718843262 40.0 36.0 41.0 31.0 41.0 56-57 36.85691095273405 39.0 36.0 41.0 31.0 41.0 58-59 36.62987382264084 39.0 35.0 41.0 31.0 41.0 60-61 36.370332387564886 39.0 35.0 41.0 31.0 41.0 62-63 36.12783868417666 38.5 35.0 41.0 30.5 41.0 64-65 35.828462830074216 37.5 35.0 40.0 30.0 41.0 66-67 35.499234295239084 37.0 35.0 40.0 30.0 41.0 68-69 35.165017802099484 36.5 34.5 39.5 29.0 41.0 70-71 34.89807823118829 36.0 34.0 39.0 29.5 41.0 72-73 34.69888070448515 36.0 34.0 39.0 30.0 41.0 74-75 34.36526139365007 35.0 34.0 38.0 29.0 40.0 76-77 34.036714608752135 35.0 34.0 37.0 29.0 39.0 78-79 33.721509593523834 35.0 34.0 37.0 29.0 39.0 80-81 33.42965106659395 35.0 34.0 36.0 29.0 38.5 82-83 33.169088998241236 35.0 34.0 36.0 29.0 37.0 84-85 32.95161873479468 35.0 34.0 35.5 29.0 37.0 86-87 32.767274777397155 35.0 34.0 35.0 29.0 36.5 88-89 32.62023556375358 35.0 34.0 35.0 29.0 36.0 90-91 32.482406561958044 35.0 33.0 35.0 28.0 36.0 92-93 32.36525312072949 35.0 33.0 35.0 27.5 36.0 94-95 32.28894462045679 35.0 33.0 35.0 27.0 35.5 96-97 32.20985090358677 35.0 33.0 35.0 27.0 35.0 98-99 32.139172585379605 35.0 33.0 35.0 27.0 35.0 100-101 32.080379696414454 35.0 33.0 35.0 27.0 35.0 102-103 31.096527518185106 34.0 31.5 35.0 24.5 35.0 104-105 31.823743588486547 35.0 33.0 35.0 25.5 35.0 106-107 32.12268863790959 35.0 33.0 35.0 27.0 35.0 108-109 32.141932064001765 35.0 34.0 35.0 27.0 35.0 110-111 32.11312330328526 35.0 34.0 35.0 27.0 35.0 112-113 32.04310926996072 35.0 33.5 35.0 27.0 35.0 114-115 31.969465262925674 35.0 33.0 35.0 27.0 35.0 116-117 31.916635924085227 35.0 33.0 35.0 27.0 35.0 118-119 31.810992566627654 35.0 33.0 35.0 27.0 35.0 120-121 31.67747743331107 35.0 33.0 35.0 25.0 35.0 122-123 31.532402885104453 35.0 33.0 35.0 25.0 35.0 124-125 30.83681388379917 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07520478780239159 1101 2 0.1341234646695071 1101 3 0.12018392038149273 1101 4 0.050602574227212926 1101 5 0.15441878816152155 1101 6 0.15440915563873858 1101 7 0.18518884964373683 1101 8 0.15951578294921376 1101 9 0.12550959693345476 1101 10-11 0.14340278417165564 1101 12-13 0.19576310169552613 1101 14-15 0.2525876072415514 1101 16-17 0.2500848757127301 1101 18-19 0.22339800787641906 1101 20-21 0.20789749224532272 1101 22-23 0.15362918902636835 1101 24-25 0.2365819206537907 1101 26-27 0.19864543377256183 1101 28-29 0.16792650273472987 1101 30-31 0.17817237276715758 1101 32-33 0.029700447811464414 1101 34-35 0.1521708061416973 1101 36-37 0.3074185385566608 1101 38-39 0.12249597780224519 1101 40-41 0.16635908767356256 1101 42-43 0.21910045620347773 1101 44-45 0.2699325593949524 1101 46-47 0.22769442107563265 1101 48-49 0.03352574471126957 1101 50-51 0.009889389320122177 1101 52-53 0.0020324329471606006 1101 54-55 0.029390783329397152 1101 56-57 0.07022299643805496 1101 58-59 -0.05592811865837888 1101 60-61 0.05507060364699612 1101 62-63 -0.05239227729221341 1101 64-65 -0.1508373468448383 1101 66-67 -0.03108907092314439 1101 68-69 0.012733972310215336 1101 70-71 0.03629681480197888 1101 72-73 -0.029149698148245307 1101 74-75 -0.02015494784563998 1101 76-77 0.019790340649002758 1101 78-79 -0.22544932519883787 1101 80-81 -0.230140050027984 1101 82-83 -0.18751924441301782 1101 84-85 -0.32251258294011365 1101 86-87 -0.3058181876732249 1101 88-89 -0.3016008291311181 1101 90-91 -0.7495468505461531 1101 92-93 -0.5399608435519454 1101 94-95 -0.38900533425575645 1101 96-97 -0.24686334421956246 1101 98-99 -0.21043342991458758 1101 100-101 -0.35177158344948367 1101 102-103 -0.26736615178185374 1101 104-105 -0.11309775957473178 1101 106-107 -0.09280399628487146 1101 108-109 -0.07532878244177255 1101 110-111 -0.08252334802077144 1101 112-113 -0.17625326335450353 1101 114-115 -0.3253639634271117 1101 116-117 -0.2510132761656827 1101 118-119 -0.24291239544938747 1101 120-121 -0.18213410913710248 1101 122-123 0.009534837934982221 1101 124-125 0.053570859292296547 1102 1 0.09132837514161452 1102 2 0.11370150367389442 1102 3 0.11845722013030269 1102 4 0.1475763831835195 1102 5 0.15438074783031652 1102 6 0.1415171587547448 1102 7 0.1994593031671812 1102 8 0.23518002318180464 1102 9 0.3059247805019467 1102 10-11 0.26585727497977985 1102 12-13 0.23050752304419575 1102 14-15 0.25144253814137585 1102 16-17 0.31520652299506224 1102 18-19 0.3215872697341169 1102 20-21 0.27796355562051644 1102 22-23 0.3371279365438724 1102 24-25 0.3745534668535839 1102 26-27 0.36191600545992486 1102 28-29 0.3617858192988308 1102 30-31 0.32235505971195266 1102 32-33 0.21505692338764248 1102 34-35 0.3156195425686974 1102 36-37 0.29699668230978915 1102 38-39 0.3104548163261853 1102 40-41 0.3093494767011862 1102 42-43 0.26393604019515493 1102 44-45 0.20664254487163447 1102 46-47 0.28546518783456065 1102 48-49 0.24342043915092404 1102 50-51 0.1553297043161166 1102 52-53 0.07763409959147083 1102 54-55 0.16236186367296312 1102 56-57 0.06111417481174186 1102 58-59 0.07374668428994369 1102 60-61 -0.014575178387786991 1102 62-63 -0.022536681697317817 1102 64-65 0.022925373005769245 1102 66-67 -6.489038617729648E-4 1102 68-69 -0.09789907209425053 1102 70-71 -0.08605301915289232 1102 72-73 0.02229240713231917 1102 74-75 0.012869848385903992 1102 76-77 0.04090309200692133 1102 78-79 -0.011210682213224743 1102 80-81 -0.03140743625110076 1102 82-83 -0.024897563592716665 1102 84-85 0.04062795329624436 1102 86-87 -4.521854749768295E-4 1102 88-89 -0.03185068427463733 1102 90-91 0.002435703092089625 1102 92-93 0.028147429154230963 1102 94-95 -0.021772613984317957 1102 96-97 -0.029891586133288683 1102 98-99 0.04234888952753124 1102 100-101 0.050004330331645974 1102 102-103 0.006202268348058482 1102 104-105 -0.04402846313892539 1102 106-107 -0.07014509842169048 1102 108-109 -0.11840523082738486 1102 110-111 -0.027176687585125592 1102 112-113 -0.09029479494277126 1102 114-115 -0.09904953675095385 1102 116-117 -0.04436900857713155 1102 118-119 -0.12231186542077666 1102 120-121 -0.11237328726012663 1102 122-123 -0.10228554260432077 1102 124-125 -0.4297662811369882 1103 1 0.06933062247959043 1103 2 0.12187976726389849 1103 3 0.09175310143395876 1103 4 0.10992599539431325 1103 5 0.05164133400106152 1103 6 0.04614996473654287 1103 7 0.07302915648126884 1103 8 0.16512015362253862 1103 9 0.2527003081192163 1103 10-11 0.2357070218614865 1103 12-13 0.20747789381893256 1103 14-15 0.3541028609516417 1103 16-17 0.29774200090209035 1103 18-19 0.27110558020581976 1103 20-21 0.24973238572381717 1103 22-23 0.27569250120907185 1103 24-25 0.17356508134270143 1103 26-27 0.21616954388222354 1103 28-29 0.2783774035459956 1103 30-31 0.3125393057538375 1103 32-33 0.3174371847195019 1103 34-35 0.28077292239457563 1103 36-37 0.17744087837119338 1103 38-39 0.278952719333212 1103 40-41 0.13694069395881314 1103 42-43 0.11640633737380313 1103 44-45 0.10330166095832993 1103 46-47 0.020229582174813743 1103 48-49 -0.02954590825105896 1103 50-51 -0.029357463679509976 1103 52-53 0.11566652042343151 1103 54-55 0.12295192591344772 1103 56-57 0.02931138781719511 1103 58-59 -0.027556479873283024 1103 60-61 0.005955156621226365 1103 62-63 0.006017428180101092 1103 64-65 -0.03161923477934181 1103 66-67 -0.19318427907658986 1103 68-69 -0.09226036887707778 1103 70-71 -0.08890443955733218 1103 72-73 -0.060686593031086034 1103 74-75 0.11188045396907853 1103 76-77 0.06495288728008575 1103 78-79 0.08399157370045174 1103 80-81 0.08667059073243877 1103 82-83 0.0899712417023153 1103 84-85 0.062101615356347395 1103 86-87 0.02184787864459281 1103 88-89 0.0812461398876465 1103 90-91 0.06790315893939436 1103 92-93 -0.005786761713117983 1103 94-95 -0.03733832105532997 1103 96-97 -0.13641448730716377 1103 98-99 -0.06093551366947736 1103 100-101 -0.10912725027984749 1103 102-103 0.0035344327917989915 1103 104-105 -0.09744381692170023 1103 106-107 -0.12814430823215162 1103 108-109 -0.03629533106398952 1103 110-111 -0.1776332085135266 1103 112-113 -0.0629296626959217 1103 114-115 -0.15418984944333047 1103 116-117 -0.19444493775698035 1103 118-119 -0.20445077122985822 1103 120-121 -0.1253567356177463 1103 122-123 -0.1468856708434707 1103 124-125 -0.21896187142533208 1104 1 0.12329210851740413 1104 2 0.05123853510517762 1104 3 0.08818458150923902 1104 4 0.01853661845291299 1104 5 0.13238551788018782 1104 6 0.13176373004135655 1104 7 0.1845720131399773 1104 8 0.20622035283268758 1104 9 0.22964657856844184 1104 10-11 0.15531550666761262 1104 12-13 0.19520279953566444 1104 14-15 0.19454358031103425 1104 16-17 0.26640664687251103 1104 18-19 0.30004453463318725 1104 20-21 0.2047806314353977 1104 22-23 0.24871599445064163 1104 24-25 0.27454032145924145 1104 26-27 0.2078664733347182 1104 28-29 0.20322931976546244 1104 30-31 0.264804593094091 1104 32-33 0.15940904747736795 1104 34-35 0.23540601457878552 1104 36-37 0.3398379240031346 1104 38-39 0.19745871734852471 1104 40-41 0.18329544476608817 1104 42-43 0.21061706379715872 1104 44-45 0.200180701953677 1104 46-47 0.09401491391107442 1104 48-49 0.05673480331361702 1104 50-51 -0.009827773318377808 1104 52-53 0.07671331097953527 1104 54-55 0.10586311588961195 1104 56-57 0.0953554830525647 1104 58-59 0.1551603186999415 1104 60-61 0.157658292195336 1104 62-63 0.08709442327345585 1104 64-65 0.04906908341465055 1104 66-67 0.046400609758634914 1104 68-69 0.03830117922194631 1104 70-71 0.05841416641643349 1104 72-73 0.19373209141939896 1104 74-75 0.17092942408120138 1104 76-77 0.12385332350901024 1104 78-79 0.15815393261973298 1104 80-81 0.15735021400180216 1104 82-83 0.05748547818519967 1104 84-85 0.2124271719745039 1104 86-87 0.209778719574075 1104 88-89 0.2526055241048226 1104 90-91 0.2202631796547081 1104 92-93 0.2315081635645413 1104 94-95 0.24239281408244295 1104 96-97 0.16333986253805222 1104 98-99 0.11020173163859681 1104 100-101 0.03723025409674108 1104 102-103 0.043968480040934566 1104 104-105 0.08320568653561722 1104 106-107 0.06569849896821722 1104 108-109 0.11849921559788612 1104 110-111 0.09371886815431196 1104 112-113 -0.0021372066285891833 1104 114-115 -0.026254253311460474 1104 116-117 -0.017323974516070706 1104 118-119 -0.09254121327304787 1104 120-121 -0.11978419971808663 1104 122-123 -0.16335942879837262 1104 124-125 -0.3878184199956465 1105 1 0.09969532017144189 1105 2 0.022809484291727244 1105 3 0.04034575966084475 1105 4 0.036620780226513716 1105 5 -0.01323546375207485 1105 6 0.04618566690902526 1105 7 0.07540659660304527 1105 8 0.10778246462415808 1105 9 0.11881012908364141 1105 10-11 0.1433184545626247 1105 12-13 0.080026277024416 1105 14-15 0.1843349737575295 1105 16-17 0.21942090097225275 1105 18-19 0.23662810043298776 1105 20-21 0.10256640794680294 1105 22-23 0.11232395796048422 1105 24-25 0.18502710153390467 1105 26-27 0.1583644809895972 1105 28-29 0.13365134469258066 1105 30-31 0.10846866305523406 1105 32-33 0.13449005693222915 1105 34-35 0.18880710127436373 1105 36-37 0.12550016777895934 1105 38-39 0.24980324786059782 1105 40-41 0.1803658954732299 1105 42-43 0.12106898700512403 1105 44-45 0.09676221303292465 1105 46-47 0.070053045750754 1105 48-49 0.1372308401140785 1105 50-51 0.1581335769684955 1105 52-53 0.17170351436542575 1105 54-55 0.13531245077866316 1105 56-57 0.1252650334147063 1105 58-59 -0.05513613764091474 1105 60-61 -0.02185142631843462 1105 62-63 0.06185778970245792 1105 64-65 -0.15926883634850952 1105 66-67 -0.02830840092485687 1105 68-69 -0.07437980129909505 1105 70-71 -0.0893737067485958 1105 72-73 0.03030580294711882 1105 74-75 0.009814434160865915 1105 76-77 0.05048512282571238 1105 78-79 0.1338791389042413 1105 80-81 0.17067104079619355 1105 82-83 0.053183669087609076 1105 84-85 0.006628823927904648 1105 86-87 0.03569383026166406 1105 88-89 0.07680635836330652 1105 90-91 0.010247936982779038 1105 92-93 -0.04586731879683725 1105 94-95 -0.010559798400400666 1105 96-97 0.05032734866082933 1105 98-99 -0.02825314994764483 1105 100-101 0.05434785854416191 1105 102-103 0.08128105230996496 1105 104-105 0.08210808835719163 1105 106-107 0.09847496109584597 1105 108-109 0.11736492598083004 1105 110-111 0.07556551681072676 1105 112-113 0.07380830553671558 1105 114-115 0.15961304318995317 1105 116-117 0.14162988966899448 1105 118-119 -0.03321666072514162 1105 120-121 0.07104875883841189 1105 122-123 3.273688881790804E-4 1105 124-125 -0.0709409348058827 1106 1 0.08952193194457436 1106 2 0.08606194108705267 1106 3 0.05647277480919399 1106 4 0.09676070511343227 1106 5 0.11133625295639149 1106 6 0.033740201995072994 1106 7 0.10688994594782031 1106 8 0.06379719815222984 1106 9 0.06888572342636223 1106 10-11 0.07573058436141622 1106 12-13 0.07397823002953174 1106 14-15 0.15836520716494817 1106 16-17 0.09422254804466945 1106 18-19 0.052080653180432535 1106 20-21 0.11354261658618725 1106 22-23 0.05641270350404426 1106 24-25 0.04016173007084234 1106 26-27 0.04904352118207811 1106 28-29 0.03601574322922119 1106 30-31 0.07761894400906755 1106 32-33 0.19335764218797635 1106 34-35 0.17938149671529402 1106 36-37 0.1703939984845917 1106 38-39 0.10814108155104663 1106 40-41 0.047247580127177 1106 42-43 0.1230099273324683 1106 44-45 0.09835977736138801 1106 46-47 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-0.5988637278322884 2216 32-33 -0.5947209622977994 2216 34-35 -0.6899680736985303 2216 36-37 -0.513276971853692 2216 38-39 -0.558938380177608 2216 40-41 -0.8345611609937436 2216 42-43 -0.597661754159077 2216 44-45 -0.5683413673748419 2216 46-47 -0.6177961245128643 2216 48-49 -0.6339863035365241 2216 50-51 -0.4782633699797856 2216 52-53 -0.6251966070594079 2216 54-55 -0.495112521376349 2216 56-57 -0.48887453424196536 2216 58-59 -0.5511258682314022 2216 60-61 -0.6435203678542933 2216 62-63 -0.5341628141128183 2216 64-65 -0.3983047792817871 2216 66-67 -0.42210266116399 2216 68-69 -0.567571442779709 2216 70-71 -0.6366136552242878 2216 72-73 -0.5771209802401174 2216 74-75 -0.6467467169820367 2216 76-77 -0.5980317806341588 2216 78-79 -0.6524615271892742 2216 80-81 -0.5500262804714708 2216 82-83 -0.5181273498469778 2216 84-85 -0.5757730686450913 2216 86-87 -0.5320590125862381 2216 88-89 -0.5307823246056032 2216 90-91 -0.5903710785601959 2216 92-93 -0.5222515176144924 2216 94-95 -0.5241328645400145 2216 96-97 -0.5536162995793958 2216 98-99 -0.5594031258694869 2216 100-101 -0.4998270434444301 2216 102-103 -0.5993637253880486 2216 104-105 -0.6453434524973893 2216 106-107 -0.5127547681947249 2216 108-109 -0.408155044120889 2216 110-111 -0.3890091565091929 2216 112-113 -0.26016036626464967 2216 114-115 -0.24806604367163843 2216 116-117 -0.31147767028267026 2216 118-119 -0.20285025906596488 2216 120-121 -0.19079141830594892 2216 122-123 -0.28137958068949587 2216 124-125 -0.22333988777517888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 220.0 3 13.0 4 34.0 5 92.0 6 258.0 7 487.0 8 969.0 9 1581.0 10 2546.0 11 3410.0 12 4713.0 13 6528.0 14 9898.0 15 8279.0 16 4256.0 17 3854.0 18 3824.0 19 3485.0 20 3717.0 21 3671.0 22 3950.0 23 4525.0 24 5883.0 25 7842.0 26 10359.0 27 13756.0 28 17860.0 29 22952.0 30 28849.0 31 36838.0 32 46428.0 33 61108.0 34 84404.0 35 128943.0 36 261822.0 37 564348.0 38 269069.0 39 1059.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.068486466738754 18.273457938883944 12.94762496720584 24.710430627171466 2 16.173927431166238 20.25076141509839 38.28548316920267 25.289827984532703 3 18.852760397835556 24.38372869722949 30.004105819846433 26.759405085088524 4 13.011894621376003 16.374315952029317 36.51446535484701 34.099324071747674 5 13.31185613629967 38.14230012441159 34.48782464894655 14.058019090342192 6 32.85065577015837 36.39737360971762 16.764903992601095 13.987066627522918 7 28.689201693803888 32.66810881035402 20.814300509244223 17.82838898659787 8 26.93803888885484 34.83163074584975 19.55405893996311 18.676271425332295 9 26.959242077912528 15.120386314750926 19.30231703057304 38.61805457676351 10-11 25.36722575268257 25.91765073567712 27.606153827298186 21.108969684342117 12-13 26.06040457645711 23.3745243070663 27.899781227211168 22.665289889265424 14-15 22.816653695544268 24.856755912074174 25.564550228884137 26.762040163497424 16-17 22.69911694232855 28.406849978245287 26.180760250761416 22.713272828664753 18-19 22.75408590355613 26.654185791412093 28.355343387485217 22.23638491754656 20-21 23.066905989421674 25.969388911937358 28.625430269262615 22.33827482937836 22-23 23.1263673299302 25.821929980451397 28.42076074100856 22.63094194860984 24-25 22.904820296734524 26.119097037741085 28.387349409772717 22.588733255751674 26-27 22.648467752857393 26.495738804364944 28.234840996458555 22.620952446319105 28-29 22.598277325948548 26.421852608446933 28.386924198603587 22.592945867000935 30-31 22.598011428921605 26.053559664792488 28.475112220979575 22.873316685306328 32-33 22.514692900680863 26.227957584925594 28.345831095297875 22.91151841909567 34-35 23.148414550706892 26.17689586104388 27.955365298167788 22.71932429008144 36-37 23.147999485240497 25.851804415901164 28.013518565046603 22.986677533811733 38-39 22.78325560873375 26.02841595019089 28.173860022183682 23.014468418891674 40-41 23.014058173463905 25.82143383503087 28.19172258815505 22.972785403350173 42-43 22.980365601809012 25.81013340850456 28.100231028967475 23.109269960718947 44-45 22.9119735658415 26.099472493424553 27.916974092857828 23.071579847876126 46-47 23.178578650962418 26.375020682301464 27.352144524858595 23.094256141877523 48-49 23.299991267432578 26.352927478325615 27.092805743271708 23.254275510970096 50-51 23.191416998094162 26.43997842912558 27.267699453987245 23.100905118793012 52-53 23.402100709019933 26.308500272699973 27.14547471243941 23.14392430584068 54-55 22.965106659394667 26.33907943842189 27.226794457756014 23.46901944442742 56-57 23.003129006243306 26.632800760864505 27.289089291273346 23.074980941618847 58-59 23.174885358698734 26.167233208871764 27.157441128941816 23.500440303487682 60-61 22.89476320902398 26.170932686288612 27.709359341769467 23.22494476291794 62-63 22.88136631879546 26.285428016398765 27.630053375658004 23.203152289147766 64-65 22.681743809097764 26.175428813050377 27.523302059650824 23.61952531820104 66-67 22.653983564464436 26.560701788789277 27.275022520728264 23.51029212601803 68-69 22.663002879594465 26.2339696548715 27.558436035192457 23.544591430341573 70-71 22.724498369006028 26.229005893394337 27.362612580867946 23.68388315673169 72-73 22.81468035970736 26.294707188539956 27.148014816526146 23.742597635226538 74-75 22.72684654651526 26.531195038698886 27.1238119166825 23.618146498103357 76-77 22.992220993879258 26.298022830837564 27.119126525589703 23.590629649693472 78-79 22.90689682960138 26.35082280348529 27.198950625662793 23.543329741250542 80-81 22.831346140470377 26.681086109443754 26.96046664331857 23.527101106767304 82-83 22.79382656281598 26.568239338656603 27.008481275623076 23.629452822904344 84-85 22.849881942286846 26.395290192783683 26.998570315884812 23.75625754904466 86-87 22.992928184921226 26.33923264065497 26.956974684863006 23.7108644895608 88-89 22.837887525048565 26.458148213968368 26.865666153949856 23.838298107033207 90-91 22.801915640722378 26.578258764699754 26.927529215665846 23.69229637891202 92-93 22.843065760526528 26.078513080406662 27.082753718218193 23.995667440848617 94-95 22.68756549395464 26.098429370706505 27.035015902391795 24.17898923294706 96-97 22.89628821629704 26.072599474209934 26.99015828854721 24.040954020945808 98-99 22.859292945665484 25.978221336643326 26.780635073642937 24.38185064404825 100-101 22.53050946805411 26.013900349270557 27.023074408202575 24.432515774472762 102-103 22.628398791540786 25.780810501093864 27.05030548525275 24.540485222112597 104-105 22.601549140110496 25.91649081371987 26.9721293889571 24.509830657212532 106-107 22.621535331498993 26.014811591893768 26.77647181385316 24.587181262754086 108-109 22.706191789955422 25.990129954173476 26.519022481839137 24.784655774031965 110-111 22.56134480560867 26.17578565475315 26.813011717409424 24.44985782222876 112-113 22.5457002261265 26.135289827984533 26.964083268477722 24.35492667741125 114-115 22.4257735180748 26.25175416556872 26.66175398172604 24.660718334630445 116-117 22.275512767874105 26.254266682191158 26.832329348032573 24.63789120190216 118-119 22.24416759098681 26.46973030278889 26.368249143599513 24.917852962624785 120-121 22.187615380962082 26.637609176243128 26.567993761576396 24.606781681218393 122-123 22.185848373548666 26.681321065228108 26.50041870439715 24.632411856826078 124-125 22.103946585073125 26.68984516448879 26.50670704672703 24.69950120371105 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 52.0 2 106.0 3 158.0 4 102.5 5 87.5 6 245.0 7 603.0 8 1025.5 9 1220.0 10 981.5 11 692.5 12 655.5 13 698.0 14 792.0 15 910.0 16 1083.0 17 1200.5 18 1304.0 19 1444.0 20 1578.5 21 1831.0 22 2228.5 23 2709.5 24 3408.0 25 4558.0 26 5939.5 27 7826.5 28 9740.0 29 12035.0 30 15393.0 31 18702.0 32 22540.5 33 25309.5 34 27626.5 35 30446.5 36 33699.0 37 37456.5 38 40364.5 39 42570.0 40 44478.0 41 48360.0 42 52299.5 43 55388.5 44 58984.0 45 62314.0 46 62900.5 47 63369.5 48 64216.5 49 63052.0 50 63142.5 51 62339.5 52 61215.0 53 61925.0 54 62906.0 55 59224.5 56 52293.5 57 47832.5 58 44502.0 59 39067.0 60 31980.0 61 25932.5 62 21122.5 63 16529.0 64 12432.5 65 9477.5 66 6859.5 67 5540.0 68 4142.0 69 2467.0 70 1758.0 71 1255.0 72 925.0 73 699.5 74 572.0 75 499.0 76 286.0 77 102.0 78 42.0 79 23.0 80 12.0 81 4.5 82 2.5 83 1.0 84 1.5 85 1.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02708615480779248 2 0.0 3 0.0 4 0.0 5 0.058400691248475634 6 0.014523571695581036 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.8384267969089916E-4 22-23 0.0 24-25 6.128089323029973E-5 26-27 6.74089825533297E-4 28-29 3.9832580599694816E-4 30-31 3.064044661514986E-4 32-33 2.7576401953634873E-4 34-35 3.370449127666485E-4 36-37 0.0 38-39 0.0 40-41 2.1448312630604902E-4 42-43 0.0 44-45 1.2256178646059945E-4 46-47 0.0 48-49 4.596066992272479E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.2256178646059945E-4 58-59 6.128089323029973E-5 60-61 3.064044661514986E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.8384267969089916E-4 70-71 1.8384267969089916E-4 72-73 2.1448312630604902E-4 74-75 0.0 76-77 1.2256178646059945E-4 78-79 3.064044661514986E-5 80-81 3.370449127666485E-4 82-83 0.0 84-85 6.128089323029973E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.1448312630604902E-4 100-101 3.064044661514986E-5 102-103 0.0 104-105 0.0013175392044514442 106-107 0.0 108-109 0.0014707414375271933 110-111 0.004289662526120981 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.596066992272479E-4 122-123 6.434493789181471E-4 124-125 9.192133984544958E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1631830.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.82712565647779 #Duplication Level Percentage of deduplicated Percentage of total 1 82.40594347865081 56.717642266457055 2 10.82415278346396 14.899906475047755 3 3.204360051758854 6.61640675793013 4 1.3197318441311243 3.633333978754721 5 0.6429648180600999 2.2126710162658436 6 0.3882969943947005 1.6035219615142025 7 0.25734492508855744 1.2398618047288903 8 0.16474502050589937 0.9071140982110837 9 0.13196605387827118 0.8174559755402359 >10 0.6130951131784579 7.4109851848359725 >50 0.03550760241152951 1.63806748107467 >100 0.011201962937736645 1.2944478463828324 >500 1.723378849976137E-4 0.10070431554920917 >1k 5.170136549928411E-4 0.907880837707476 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3241 0.1986113749594014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2857 0.17507951195896632 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 6.128089323029973E-5 0.0 0.0 3.064044661514986E-5 0.0 18-19 6.128089323029973E-5 0.0 0.0 6.128089323029973E-5 0.0 20-21 6.128089323029973E-5 0.0 0.0 1.2256178646059945E-4 0.0 22-23 6.128089323029973E-5 0.0 0.0 3.370449127666485E-4 0.0 24-25 6.128089323029973E-5 0.0 0.0 6.74089825533297E-4 0.0 26-27 6.128089323029973E-5 0.0 0.0 0.001164336971375695 0.0 28-29 6.128089323029973E-5 0.0 0.0 0.0033704491276664848 0.0 30-31 6.128089323029973E-5 0.0 0.0 0.014002684103123487 0.0 32-33 6.128089323029973E-5 0.0 0.0 0.034041536189431496 0.0 34-35 6.128089323029973E-5 0.0 0.0 0.06517222995042377 0.0 36-37 6.128089323029973E-5 0.0 0.0 0.11977350581862081 0.0 38-39 6.128089323029973E-5 0.0 0.0 0.19542476851142582 0.0 40-41 6.128089323029973E-5 0.0 0.0 0.2768057947212639 0.0 42-43 6.128089323029973E-5 0.0 0.0 0.35677736038680496 0.0 44-45 1.8384267969089916E-4 0.0 0.0 0.45384629526359976 0.0 46-47 3.064044661514986E-4 0.0 0.0 0.5751824638595933 0.0 48-49 3.064044661514986E-4 0.0 0.0 0.7065074180521256 0.0 50-51 3.064044661514986E-4 0.0 0.0 0.8476066747148907 0.0 52-53 3.064044661514986E-4 0.0 0.0 0.9830068083072379 0.0 54-55 3.064044661514986E-4 0.0 0.0 1.1306937609922603 0.0 56-57 3.064044661514986E-4 0.0 0.0 1.3027398687363267 0.0 58-59 3.064044661514986E-4 0.0 0.0 1.492312311944259 0.0 60-61 3.064044661514986E-4 0.0 0.0 1.6984612367709873 0.0 62-63 3.064044661514986E-4 0.0 0.0 1.9034764650729548 0.0 64-65 3.064044661514986E-4 0.0 0.0 2.1154470747565615 0.0 66-67 3.064044661514986E-4 0.0 0.0 2.358946704007158 0.0 68-69 3.064044661514986E-4 0.0 0.0 2.636273386320879 0.0 70-71 3.370449127666485E-4 0.0 0.0 2.9376834596741084 0.0 72-73 3.6768535938179833E-4 0.0 0.0 3.2499708915757157 0.0 74-75 3.6768535938179833E-4 0.0 0.0 3.5545675713769205 0.0 76-77 3.6768535938179833E-4 0.0 0.0 3.8807657660418062 0.0 78-79 3.6768535938179833E-4 0.0 0.0 4.2551001023390915 0.0 80-81 4.902471458423978E-4 0.0 0.0 4.6626180423205845 0.0 82-83 4.902471458423978E-4 0.0 0.0 5.081197183530147 0.0 84-85 4.902471458423978E-4 0.0 0.0 5.500358493225397 0.0 86-87 5.208875924575477E-4 0.0 0.0 5.927302476360895 0.0 88-89 5.515280390726976E-4 0.0 0.0 6.382803355741713 0.0 90-91 5.515280390726976E-4 0.0 0.0 6.893150634563649 0.0 92-93 5.515280390726976E-4 0.0 0.0 7.412353002457364 0.0 94-95 5.515280390726976E-4 0.0 0.0 7.93900099887856 0.0 96-97 5.515280390726976E-4 0.0 0.0 8.480478971461487 0.0 98-99 5.515280390726976E-4 0.0 0.0 9.032282774553721 0.0 100-101 5.515280390726976E-4 0.0 0.0 9.640311797184754 0.0 102-103 5.821684856878475E-4 0.0 0.0 10.287713793716257 0.0 104-105 7.353707187635967E-4 0.0 0.0 10.924851240631684 0.0 106-107 8.272920586090463E-4 0.0 0.0 11.552367587309952 0.0 108-109 8.579325052241962E-4 0.0 0.0 12.211688717574749 0.0 110-111 8.579325052241962E-4 0.0 0.0 12.90848311404987 0.0 112-113 8.579325052241962E-4 0.0 0.0 13.643792551920237 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2520 0.0 72.7815 1 GTATCAA 4535 0.0 55.674892 1 ATCAACG 5770 0.0 42.926132 3 TATCAAC 5855 0.0 42.506332 2 TCAACGC 5845 0.0 42.375324 4 CAACGCA 5940 0.0 41.697605 5 AACGCAG 6035 0.0 41.115925 6 GTACATG 6600 0.0 36.99225 1 ACGCAGA 7160 0.0 34.734524 7 CGCAGAG 7240 0.0 34.43289 8 TACATGG 7095 0.0 33.73478 2 ACATGGG 6980 0.0 33.522884 3 GCAGAGT 8140 0.0 30.260353 9 GAGTACT 4880 0.0 27.737013 12-13 GTGGTAT 1355 0.0 27.686756 1 CATGGGG 4170 0.0 27.556591 4 CATGGGA 3760 0.0 26.44435 4 CAGAGTA 8050 0.0 26.274942 10-11 TAAGGTG 795 0.0 25.46338 5 GAGTACA 5310 0.0 25.456705 1 >>END_MODULE