FastQCFastQC Report
Thu 26 May 2016
SRR1364378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381355
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.049.4171681
GGTATCA12100.049.1943971
ATCAACG21150.040.800393
CAACGCA21600.039.6748625
TCAACGC22200.038.8706444
AACGCAG22900.037.9382136
TATCAAC23550.036.6423872
GTACATG53200.036.587871
ACATGGG53300.035.171433
TACATGG56550.033.7815132
ACGCAGA27800.030.6046817
CGCAGAG28400.029.747538
CATGGGG32800.029.0303864
GGTAAGG6750.026.449913
CATGGGA27400.026.2809734
TAAGGTG6900.025.8749085
GTAAGGT6800.024.5050624
GAGTACA41250.024.0986961
GCAGAGT35850.023.5656859
ATGGGGA20800.023.1754595
Produced by FastQC (version 0.11.5)