##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364378_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1381355 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.891304552414116 31.0 31.0 34.0 28.0 34.0 2 31.000937485295236 31.0 31.0 34.0 27.0 34.0 3 31.09240636910859 31.0 31.0 34.0 27.0 34.0 4 34.53314535365637 37.0 35.0 37.0 32.0 37.0 5 34.588113844739404 37.0 35.0 37.0 32.0 37.0 6 34.73933275660493 37.0 35.0 37.0 32.0 37.0 7 34.71176489751005 37.0 35.0 37.0 32.0 37.0 8 34.67650821114051 37.0 35.0 37.0 32.0 37.0 9 36.24806440053426 39.0 37.0 39.0 32.0 39.0 10-11 36.15852514378997 39.0 37.0 39.0 31.5 39.0 12-13 36.16036753767135 39.0 37.0 39.0 32.0 39.0 14-15 37.47124852047446 40.0 38.0 41.0 32.0 41.0 16-17 37.46828114423881 40.0 38.0 41.0 32.0 41.0 18-19 37.46258130603646 40.0 38.0 41.0 32.0 41.0 20-21 37.48600251202623 40.0 38.0 41.0 31.0 41.0 22-23 37.455329006663746 40.0 38.0 41.0 31.0 41.0 24-25 37.4658027806031 40.0 38.0 41.0 31.0 41.0 26-27 37.41569690629853 40.0 38.0 41.0 31.0 41.0 28-29 37.41814776071321 40.0 38.0 41.0 31.0 41.0 30-31 37.330374161602194 40.0 38.0 41.0 30.5 41.0 32-33 37.29088322697641 40.0 38.0 41.0 30.0 41.0 34-35 37.26992988768275 40.0 38.0 41.0 30.0 41.0 36-37 37.213670273029024 40.0 37.0 41.0 30.0 41.0 38-39 37.11456251289495 40.0 37.0 41.0 30.0 41.0 40-41 37.04962410097332 40.0 37.0 41.0 30.0 41.0 42-43 36.92237802737168 40.0 37.0 41.0 30.0 41.0 44-45 36.792689786477766 40.0 37.0 41.0 30.0 41.0 46-47 36.66781095373746 40.0 36.0 41.0 30.0 41.0 48-49 36.52102573198056 40.0 36.0 41.0 30.0 41.0 50-51 36.395282892522204 40.0 36.0 41.0 29.5 41.0 52-53 36.239607124888245 39.5 35.0 41.0 29.0 41.0 54-55 36.052063010594665 39.0 35.0 41.0 29.0 41.0 56-57 35.85795939494192 39.0 35.0 41.0 29.0 41.0 58-59 35.6339829370437 39.0 35.0 41.0 28.0 41.0 60-61 35.38101682768007 38.0 35.0 40.0 28.0 41.0 62-63 35.10490315668311 38.0 34.0 40.0 28.0 41.0 64-65 34.75947964136663 37.0 34.0 40.0 28.0 41.0 66-67 34.44646090251963 37.0 34.0 40.0 27.0 41.0 68-69 34.123614132500336 36.0 34.0 39.0 26.5 41.0 70-71 33.76837091117055 36.0 33.5 39.0 26.0 41.0 72-73 33.41649250192746 35.0 33.0 38.5 26.0 40.0 74-75 33.060601004086564 35.0 33.0 37.5 26.0 39.5 76-77 32.83491173521651 35.0 33.0 37.0 26.0 39.0 78-79 32.68417676846285 35.0 33.0 36.5 26.0 39.0 80-81 32.428691031631985 35.0 33.0 36.0 26.0 38.5 82-83 32.146447147909115 35.0 33.0 36.0 26.0 37.0 84-85 31.9018300871246 35.0 33.0 35.5 25.0 37.0 86-87 31.700408294754062 35.0 33.0 35.0 25.0 36.5 88-89 31.454604717831405 35.0 32.5 35.0 24.0 36.0 90-91 31.339048253345446 35.0 32.0 35.0 24.0 36.0 92-93 31.20468525469557 35.0 32.0 35.0 24.0 36.0 94-95 31.109574656768174 35.0 32.0 35.0 23.0 35.0 96-97 31.012664014681235 35.0 32.0 35.0 23.0 35.0 98-99 30.893690615374034 35.0 32.0 35.0 22.0 35.0 100-101 30.810174430179064 35.0 32.0 35.0 20.0 35.0 102-103 29.793688081629995 33.5 30.0 34.5 19.0 35.0 104-105 30.51363009508779 34.5 31.5 35.0 19.0 35.0 106-107 30.867599929055167 35.0 32.0 35.0 21.5 35.0 108-109 30.92202728480369 35.0 33.0 35.0 21.0 35.0 110-111 30.86368384665781 35.0 33.0 35.0 20.0 35.0 112-113 30.78864846473209 35.0 33.0 35.0 19.5 35.0 114-115 30.702651382157377 35.0 32.0 35.0 19.0 35.0 116-117 30.58192499393711 35.0 32.0 35.0 17.5 35.0 118-119 30.427573650509828 35.0 32.0 35.0 11.0 35.0 120-121 30.262439416370157 35.0 32.0 35.0 2.0 35.0 122-123 30.09739784486971 35.0 32.0 35.0 2.0 35.0 124-125 29.27592110644983 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03825932995684411 1101 2 0.02199930367839542 1101 3 0.07536255794488511 1101 4 0.3170277288146366 1101 5 0.015582181201700962 1101 6 0.08815753808084992 1101 7 0.1552851395924577 1101 8 0.08220282574720272 1101 9 0.26463156912553387 1101 10-11 0.4385187141567286 1101 12-13 0.3519872978592673 1101 14-15 0.4555650263758295 1101 16-17 0.5387465595185077 1101 18-19 0.31923756816820514 1101 20-21 0.4294156024527709 1101 22-23 0.4777691291513406 1101 24-25 0.5860833075799832 1101 26-27 0.5609340222599428 1101 28-29 0.4099625133952003 1101 30-31 0.45748878687006567 1101 32-33 0.3441526920399909 1101 34-35 0.4619808713361948 1101 36-37 0.2942844148256256 1101 38-39 0.41709422367977567 1101 40-41 0.4235321445121798 1101 42-43 0.5473500400336064 1101 44-45 0.4910715069230136 1101 46-47 0.4165910017753447 1101 48-49 0.4257990286357298 1101 50-51 0.2549011752994801 1101 52-53 0.2683650610078345 1101 54-55 0.22329168803121746 1101 56-57 0.12432823342980015 1101 58-59 0.20867274022632643 1101 60-61 0.1876411170033947 1101 62-63 0.28649443421048204 1101 64-65 0.35416769192728026 1101 66-67 0.2786440515421802 1101 68-69 0.20646353317533084 1101 70-71 0.20245203974806003 1101 72-73 0.36034415577794476 1101 74-75 0.23324461618260983 1101 76-77 0.2794601581900409 1101 78-79 0.2820043487819106 1101 80-81 0.2669293091840146 1101 82-83 0.24915745954395874 1101 84-85 0.17834891995186197 1101 86-87 0.25331037268708556 1101 88-89 0.3107335781814129 1101 90-91 0.2924084011045487 1101 92-93 0.18870520777158362 1101 94-95 0.19877520447256458 1101 96-97 0.29046237858319657 1101 98-99 0.2367227056161667 1101 100-101 0.14888676340462936 1101 102-103 -0.06898068400903767 1101 104-105 0.21754888192723598 1101 106-107 0.23126026986067316 1101 108-109 0.2943042874152546 1101 110-111 0.2597163581075854 1101 112-113 0.30442507556666953 1101 114-115 0.2086797560829794 1101 116-117 0.1288571644730574 1101 118-119 0.1695710062676241 1101 120-121 -0.10803367945607789 1101 122-123 -0.1556546236081786 1101 124-125 -0.3774533889286502 1102 1 0.0924811596358488 1102 2 0.13637782813466615 1102 3 0.0070485042311325685 1102 4 0.24277432916827024 1102 5 0.2854354513021633 1102 6 0.3521673023167722 1102 7 0.3252461737590622 1102 8 0.29059348679225394 1102 9 0.3316713233368276 1102 10-11 0.45082930866636417 1102 12-13 0.3850290948331647 1102 14-15 0.33293653907314535 1102 16-17 0.46542691596390995 1102 18-19 0.4946194161588906 1102 20-21 0.41083681855618437 1102 22-23 0.36593123777223724 1102 24-25 0.41524452775101395 1102 26-27 0.278321078473752 1102 28-29 0.3129167274829996 1102 30-31 0.43660685411693123 1102 32-33 0.43397624425129777 1102 34-35 0.281170368061332 1102 36-37 0.17122626529570084 1102 38-39 0.3528328357014985 1102 40-41 0.25454494501124003 1102 42-43 0.19467016502896684 1102 44-45 0.1625454450212871 1102 46-47 0.23528038759982906 1102 48-49 0.31356221853175015 1102 50-51 0.2483785740954758 1102 52-53 0.18507829930931052 1102 54-55 0.13491556978299712 1102 56-57 0.17537763238811266 1102 58-59 0.20435620313460134 1102 60-61 0.10941234601411054 1102 62-63 0.10290705831403812 1102 64-65 -0.007254166429063957 1102 66-67 -0.033341881625176484 1102 68-69 0.06690881857834086 1102 70-71 -0.07035073956412674 1102 72-73 -0.03841886011233697 1102 74-75 9.771874965025518E-5 1102 76-77 0.07904874291602226 1102 78-79 0.13051132232929774 1102 80-81 0.14108607216954283 1102 82-83 0.09742109889255346 1102 84-85 0.09516496850817546 1102 86-87 0.17013239568919758 1102 88-89 0.17780564418708877 1102 90-91 0.0927944498774309 1102 92-93 0.14109011940828609 1102 94-95 0.10665161509522747 1102 96-97 0.07264366866021632 1102 98-99 0.09469133429913157 1102 100-101 0.10052935015047026 1102 102-103 -0.24425063560855875 1102 104-105 -0.034701057021397475 1102 106-107 -0.021230887337200244 1102 108-109 0.017752045492020585 1102 110-111 -0.06142765366804781 1102 112-113 -0.10140508736891363 1102 114-115 0.006662832967379018 1102 116-117 0.06633608276976943 1102 118-119 0.018513865423301468 1102 120-121 -0.028331211835709524 1102 122-123 0.07064058023119912 1102 124-125 -0.016016345522558595 1103 1 0.13225897499450667 1103 2 0.18468788984948148 1103 3 0.10875288079655476 1103 4 0.2935224110910255 1103 5 0.24923116918617438 1103 6 0.2565088672756062 1103 7 0.3285815813684394 1103 8 0.35609852747145965 1103 9 0.4190836867443437 1103 10-11 0.4833896326491214 1103 12-13 0.4293356546173044 1103 14-15 0.4929854643516336 1103 16-17 0.32971662088125697 1103 18-19 0.3678569685005968 1103 20-21 0.3571504406142978 1103 22-23 0.39019037498960785 1103 24-25 0.46851930871547154 1103 26-27 0.4795499574837905 1103 28-29 0.47819805725566766 1103 30-31 0.4686989350146433 1103 32-33 0.43629661485109494 1103 34-35 0.47601543094675236 1103 36-37 0.35176718498353665 1103 38-39 0.3244842263693357 1103 40-41 0.37506313088490373 1103 42-43 0.3140044354765621 1103 44-45 0.27557009279083644 1103 46-47 0.2887312307906811 1103 48-49 0.26939697959823405 1103 50-51 0.25394590900563685 1103 52-53 0.31682077597606195 1103 54-55 0.307497181396549 1103 56-57 0.29242415379892606 1103 58-59 0.2676116514066962 1103 60-61 0.2112265995408933 1103 62-63 0.2774048628946275 1103 64-65 0.2968985703198683 1103 66-67 0.2984801822339449 1103 68-69 0.18714306730963415 1103 70-71 0.25160288929622965 1103 72-73 0.2641125731778615 1103 74-75 0.09141454172503671 1103 76-77 0.06364429912898828 1103 78-79 0.23939208945656532 1103 80-81 0.28572019013193284 1103 82-83 0.2486020230272814 1103 84-85 0.05540729137073441 1103 86-87 0.13853847078992487 1103 88-89 0.1814786908620718 1103 90-91 0.004389896331197463 1103 92-93 0.044313836438721665 1103 94-95 0.05564621145697046 1103 96-97 -0.06159041237033236 1103 98-99 -0.09954569562649951 1103 100-101 -0.01938315811091229 1103 102-103 -0.21936179623430974 1103 104-105 -0.13238991938383649 1103 106-107 -0.05373856930310694 1103 108-109 -0.064100166815237 1103 110-111 -0.2223697267224516 1103 112-113 -0.2018169669880443 1103 114-115 -0.17743748787805913 1103 116-117 -0.25611216906775525 1103 118-119 -0.2781737151590278 1103 120-121 -0.3814538609044753 1103 122-123 -0.3345215878002321 1103 124-125 -0.36601144639973526 1104 1 0.20601918789619944 1104 2 0.21126320870268955 1104 3 0.17654871806866979 1104 4 0.28065131629897166 1104 5 0.23412380404071342 1104 6 0.25502843258915675 1104 7 0.28538996209255174 1104 8 0.28477226502069186 1104 9 0.3261254759065437 1104 10-11 0.4124306858120761 1104 12-13 0.3677886288705565 1104 14-15 0.3441291588581308 1104 16-17 0.37151456126896676 1104 18-19 0.35645559217287115 1104 20-21 0.4462248189480178 1104 22-23 0.45001197497172996 1104 24-25 0.400666356259471 1104 26-27 0.4659889203906147 1104 28-29 0.4846902638261241 1104 30-31 0.42615758521754543 1104 32-33 0.47571783397412304 1104 34-35 0.4538541695881264 1104 36-37 0.31144340915231083 1104 38-39 0.33380312153139613 1104 40-41 0.4402647303836602 1104 42-43 0.3111212017128935 1104 44-45 0.24425113561149914 1104 46-47 0.15036633476312034 1104 48-49 0.2253043996472357 1104 50-51 0.11237002242065586 1104 52-53 0.17236917978338084 1104 54-55 0.2522487862078151 1104 56-57 0.11054375778207515 1104 58-59 0.11940493137780095 1104 60-61 0.08433592735103446 1104 62-63 0.10712021723448828 1104 64-65 0.24640202162741076 1104 66-67 0.30324525300433436 1104 68-69 0.1503229261017438 1104 70-71 0.03692868752937528 1104 72-73 0.04726480235726882 1104 74-75 -0.0027890309266211943 1104 76-77 0.08697077864025005 1104 78-79 0.10423419390370725 1104 80-81 0.11805167069886835 1104 82-83 0.10102311401165309 1104 84-85 0.054001756236804255 1104 86-87 0.04014120721144465 1104 88-89 0.05807831501908112 1104 90-91 -0.041076868103893105 1104 92-93 -0.1280030153449836 1104 94-95 -0.1323036703681808 1104 96-97 -0.2762461457543779 1104 98-99 -0.2569211697053255 1104 100-101 -0.2636223907017232 1104 102-103 -0.552695471574733 1104 104-105 -0.33748142452055774 1104 106-107 -0.2877403896928392 1104 108-109 -0.2436473904700911 1104 110-111 -0.33860265999868844 1104 112-113 -0.3538501763324078 1104 114-115 -0.38211801449601523 1104 116-117 -0.4201713736869479 1104 118-119 -0.49830885812026793 1104 120-121 -0.6472695227315128 1104 122-123 -0.5556278028898589 1104 124-125 -0.6658224988832337 1105 1 -0.36027092523589843 1105 2 -0.28552005061327534 1105 3 -0.36416564130094997 1105 4 -0.25354588799420696 1105 5 -0.3308517798401027 1105 6 -0.21831526492361775 1105 7 -0.1277250505874008 1105 8 -0.21767175005892625 1105 9 -0.27075753645181777 1105 10-11 -0.18796203444421167 1105 12-13 -0.056344550835234486 1105 14-15 0.013070090026346293 1105 16-17 -0.008491988438024123 1105 18-19 -0.04901867827104667 1105 20-21 -0.08416348386079875 1105 22-23 -0.04359823750268532 1105 24-25 -0.12335651834094818 1105 26-27 -0.036059774649089604 1105 28-29 -0.026482360932895688 1105 30-31 -0.10735690278674781 1105 32-33 -0.12140352829501921 1105 34-35 -0.1926279080715645 1105 36-37 -0.23047602639315556 1105 38-39 -0.24642357960097883 1105 40-41 -0.1616894985685775 1105 42-43 -0.1972511939156263 1105 44-45 -0.1972307934891333 1105 46-47 -0.16821180643091083 1105 48-49 -0.2963961472248968 1105 50-51 -0.26352886915393725 1105 52-53 -0.23372161077805487 1105 54-55 -0.28619884584808375 1105 56-57 -0.4508548296833865 1105 58-59 -0.5384808022330745 1105 60-61 -0.4471997757615327 1105 62-63 -0.3653788783029768 1105 64-65 -0.29765786602698796 1105 66-67 -0.47189673013683375 1105 68-69 -0.5178432040045351 1105 70-71 -0.5111407655810112 1105 72-73 -0.4445925223910976 1105 74-75 -0.4410482114398704 1105 76-77 -0.3991960785315669 1105 78-79 -0.294731987936089 1105 80-81 -0.40315087560349383 1105 82-83 -0.299518012380652 1105 84-85 -0.367144621631045 1105 86-87 -0.36636707801496726 1105 88-89 -0.3827015632673536 1105 90-91 -0.36445270243781636 1105 92-93 -0.33689945307339286 1105 94-95 -0.425117628104001 1105 96-97 -0.4357257215046424 1105 98-99 -0.49802215404021055 1105 100-101 -0.5498179328811368 1105 102-103 -0.6566835396602215 1105 104-105 -0.6169350068039243 1105 106-107 -0.6390078606757577 1105 108-109 -0.5341332310434943 1105 110-111 -0.5225053459465734 1105 112-113 -0.5670315599591014 1105 114-115 -0.6464787604722488 1105 116-117 -0.7396756058725309 1105 118-119 -0.6057256912604743 1105 120-121 -0.740136961730542 1105 122-123 -0.7466345614534156 1105 124-125 -0.8400808614477491 1106 1 0.0023930610794948848 1106 2 0.057979087364206094 1106 3 0.08893999686509702 1106 4 0.17706466639402407 1106 5 0.17684076097127388 1106 6 0.17766454489266437 1106 7 0.10917793147175558 1106 8 0.2546088382714444 1106 9 0.36373649673604547 1106 10-11 0.36776300731000333 1106 12-13 0.28518894008659856 1106 14-15 0.32834893410802835 1106 16-17 0.29636597988265123 1106 18-19 0.23522314598411498 1106 20-21 0.12385080012173688 1106 22-23 0.2891390880724387 1106 24-25 0.254924049785771 1106 26-27 0.27759014992393816 1106 28-29 0.29626594196026446 1106 30-31 0.3657141534602175 1106 32-33 0.2684276365129179 1106 34-35 0.31744866377263037 1106 36-37 0.22905100609432338 1106 38-39 0.247644122839624 1106 40-41 0.2234106442039021 1106 42-43 0.23135835702452567 1106 44-45 0.18352472210809623 1106 46-47 0.12192443353460902 1106 48-49 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0.06089073782420229 2216 38-39 -0.11569133044437052 2216 40-41 -0.08358712021176018 2216 42-43 0.17808496047810962 2216 44-45 0.14309629935138446 2216 46-47 0.22297862718246364 2216 48-49 0.14002252748425548 2216 50-51 0.07244949488320884 2216 52-53 0.15813683297325554 2216 54-55 0.18562759015878072 2216 56-57 0.1436234690142939 2216 58-59 0.16890625504900925 2216 60-61 0.08187951368888946 2216 62-63 -0.04835863338311697 2216 64-65 0.07196000982113304 2216 66-67 0.01635914439762587 2216 68-69 0.1490967851284779 2216 70-71 0.06670498339848763 2216 72-73 0.16832504842485463 2216 74-75 0.21308180207508798 2216 76-77 0.19426795380802986 2216 78-79 -0.005897773093018088 2216 80-81 0.1986349123733575 2216 82-83 0.21987126128514234 2216 84-85 0.17156428757803965 2216 86-87 0.07629839420028262 2216 88-89 0.19046424596201916 2216 90-91 0.06583029485575409 2216 92-93 0.06956257781498465 2216 94-95 0.19263162479071028 2216 96-97 0.21395733906505043 2216 98-99 0.060197680616536076 2216 100-101 0.17974026911412366 2216 102-103 0.17566010126154197 2216 104-105 0.1645362661809493 2216 106-107 0.24107615213077693 2216 108-109 0.2876774289195758 2216 110-111 0.2407858634870692 2216 112-113 0.2342289760309093 2216 114-115 0.3491187776051845 2216 116-117 0.2650726824052221 2216 118-119 0.24996799900979028 2216 120-121 0.44834055117400595 2216 122-123 0.3658499204217591 2216 124-125 0.3636383731175208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22958.0 3 4123.0 4 2720.0 5 2238.0 6 2391.0 7 2810.0 8 3139.0 9 3461.0 10 3739.0 11 4202.0 12 4682.0 13 4686.0 14 4671.0 15 4415.0 16 4090.0 17 3714.0 18 3546.0 19 3512.0 20 3437.0 21 3626.0 22 4304.0 23 5713.0 24 8117.0 25 11918.0 26 14318.0 27 14413.0 28 16892.0 29 21274.0 30 25112.0 31 31168.0 32 40541.0 33 54581.0 34 76974.0 35 119462.0 36 236781.0 37 432573.0 38 174609.0 39 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.185275123680185 18.50310585546275 12.763519723286636 24.54809929757043 2 15.788216249245254 19.807161297889316 39.55039536422594 24.854227088639487 3 19.00937430546959 24.307243241834495 29.073595066779887 27.60978738591603 4 12.55320211773206 16.203343398810823 37.25440512448879 33.98904935896832 5 13.621612228199876 38.23260225766978 34.485145575532016 13.660639938598331 6 32.984857712023526 36.32078101793888 17.33734404949575 13.357017220541836 7 29.179842469521905 32.03414125618973 21.030753192598382 17.75526308168998 8 26.493262050667642 35.38757234744146 19.72208447502633 18.397081126864563 9 26.440849745358726 16.202062467649515 19.490355484288976 37.86673230270278 10-11 24.862770364552134 26.508874454565362 27.917457709020688 20.710897471861816 12-13 25.434757113713722 23.89285522960938 27.686473917468902 22.985913739207987 14-15 22.505157997611033 25.712020849169292 24.801100372823687 26.98172078039599 16-17 23.003344183802334 28.827595543066998 25.665696724263558 22.50336354886711 18-19 23.032220560406675 27.763230858997765 26.72005148571619 22.484497094879373 20-21 23.43134096593562 27.274741105653504 26.687455433252133 22.606462495158738 22-23 23.428952007268226 26.956177086990674 26.575282964914887 23.039587940826216 24-25 23.177025456888344 27.19275638774971 26.616763974503293 23.013454180858652 26-27 22.89469397801434 27.47259031892598 26.441320297823513 23.19139540523616 28-29 23.097226485356376 27.3872264274421 26.55597559492439 22.95957149227713 30-31 23.014854633517054 27.18808038637467 26.50614053535174 23.290924444756538 32-33 22.818634042625586 27.301302671618817 26.52376817375132 23.356295112004275 34-35 23.489082387758028 27.282664879038272 26.185546561107785 23.04270617209591 36-37 23.544644043219897 26.870256693701727 26.190127154180914 23.394972108897463 38-39 23.07262406038715 27.080323754925878 26.45600854524204 23.391043639444938 40-41 23.306937667159783 26.791860574126552 26.57272719704376 23.3284745616699 42-43 22.968901346947106 26.87031344789792 26.51569556070673 23.64508964444824 44-45 22.979417457172943 27.05274333156814 26.6381460617422 23.32969314951672 46-47 23.3565955167209 27.336455871227887 26.081094287855038 23.225854324196167 48-49 23.1581671619533 27.122607874152553 26.308588306409288 23.410636657484858 50-51 23.1518689981938 27.12568456334541 26.461228286718473 23.26121815174231 52-53 23.28606172179476 26.97439123577879 26.36838700873461 23.371160033691837 54-55 22.983743109861425 26.99616743959772 26.49603061415079 23.524058836390065 56-57 22.953003323553066 27.370556258972183 26.490223715444206 23.186216702030546 58-59 23.08762058925306 26.826014122498265 26.3606695517837 23.725695736464974 60-61 22.910013993688725 27.015420567175134 26.657796033842573 23.416769405293568 62-63 22.84064748722255 27.07425398526069 26.759161394008718 23.32593713350804 64-65 22.89361292407997 26.88763027766386 26.54031969947771 23.67843709877846 66-67 22.794711709702923 27.114324311019345 26.415932342780362 23.67503163649737 68-69 22.811293941173105 26.864194563554662 26.86394118034586 23.460570314926372 70-71 22.836683249742276 26.777456187089527 26.625681952439972 23.76017861072823 72-73 22.858883438354262 26.90804105082704 26.40594594125006 23.82712956956864 74-75 22.918594162420746 27.157854119881907 26.351491248264345 23.572060469432994 76-77 23.071596576360438 26.857421469199767 26.571466629794177 23.499515324645614 78-79 23.066242096777756 26.780806323229346 26.61310435420625 23.539847225786648 80-81 23.020191365876293 27.071531629230606 26.517791148115627 23.390485856777474 82-83 23.011022956257474 27.00293084020677 26.50472058201267 23.481325621523087 84-85 23.034358791891552 26.938729119603693 26.545961033033276 23.48095105547148 86-87 23.056382754225893 26.881123376616323 26.657318225731547 23.405175643426233 88-89 23.02252857020301 26.92290289406899 26.528054743010248 23.52651379271775 90-91 23.064633008362094 27.02361380472624 26.494603479342356 23.41714970756932 92-93 23.044783229074895 26.594372288417865 26.709550053824433 23.65129442868281 94-95 22.91367893419665 26.541814741161318 26.795227543050494 23.74927878159154 96-97 23.108558703996223 26.473807582994656 26.761713507182083 23.655920205827037 98-99 22.911613478867555 26.515051426234166 26.62139686067409 23.95193823422419 100-101 22.837584166644344 26.410825164315444 26.826436995461645 23.92515367357857 102-103 22.815879432490622 26.19377355512859 26.91677031043904 24.073576701941747 104-105 22.64193901363276 26.50941132225449 26.805572698713533 24.043076965399216 106-107 22.82933814727281 26.447647865201972 26.65550058587535 24.06751340164986 108-109 22.821591032079017 26.49259930783774 26.454483689012747 24.2313259710705 110-111 22.77243169186275 26.65147602043679 26.644707193959455 23.931385093741007 112-113 22.79065255406338 26.63820834948638 26.889815781081627 23.681323315368612 114-115 22.6530086273113 26.889636005215333 26.535407305089148 23.921948062384214 116-117 22.562821000553456 26.841754532150457 26.74416710615999 23.851257361136096 118-119 22.454368605774594 26.919987716713816 26.486958423683564 24.138685253828026 120-121 22.485297066943144 27.078592543949515 26.619205075055007 23.816905314052335 122-123 22.563401990392286 27.113981866891386 26.54501911444086 23.777597028275473 124-125 22.548672181972513 27.15112312066647 26.469739721242373 23.83046497611864 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 456.0 1 391.0 2 304.0 3 238.0 4 183.0 5 162.0 6 141.5 7 134.0 8 133.0 9 118.5 10 117.0 11 122.5 12 125.5 13 135.0 14 146.5 15 153.5 16 187.5 17 217.5 18 283.5 19 377.0 20 463.0 21 687.5 22 1018.0 23 1461.0 24 2104.5 25 3038.5 26 4422.0 27 6191.5 28 8074.0 29 10288.5 30 13185.5 31 16520.5 32 20548.5 33 23499.0 34 25202.5 35 27854.5 36 31846.0 37 35517.0 38 37741.0 39 39402.0 40 40379.5 41 42175.0 42 43874.5 43 45768.5 44 48782.0 45 51331.0 46 51452.0 47 50690.5 48 50873.0 49 50568.0 50 50706.0 51 50178.0 52 50350.0 53 51812.0 54 53378.0 55 50714.0 56 45055.0 57 41460.5 58 39095.5 59 34697.5 60 27840.0 61 22646.5 62 18557.0 63 14240.5 64 10629.0 65 8179.0 66 6124.5 67 4787.5 68 3655.5 69 2481.0 70 1747.0 71 1227.0 72 852.5 73 589.5 74 445.0 75 344.5 76 237.5 77 136.5 78 87.5 79 57.0 80 33.5 81 30.0 82 23.0 83 16.0 84 13.5 85 11.5 86 11.5 87 8.0 88 5.5 89 7.0 90 4.0 91 2.5 92 4.0 93 2.5 94 1.0 95 3.0 96 2.0 97 1.0 98 1.5 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041698187649083694 2 0.007022090628404719 3 0.0018822098591600278 4 0.00477791733479084 5 0.020631915763869534 6 0.028088362513618875 7 0.0025337440411769604 8 0.0 9 0.0 10-11 1.8098171722692575E-4 12-13 6.515341820169327E-4 14-15 3.619634344538515E-4 16-17 1.0858903033615545E-4 18-19 7.239268689077029E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.171780606723109E-4 30-31 1.0858903033615545E-4 32-33 1.0858903033615545E-4 34-35 6.515341820169327E-4 36-37 3.6196343445385145E-5 38-39 1.0858903033615545E-4 40-41 3.2576709100846636E-4 42-43 6.515341820169327E-4 44-45 0.0010496939599161693 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.325158992438583E-4 54-55 0.00238895866739542 56-57 1.4478537378154058E-4 58-59 9.411049295800139E-4 60-61 0.0013030683640338654 62-63 0.0010858903033615544 64-65 0.0019184062026054128 66-67 0.0028233147887400415 68-69 0.0034748489707569744 70-71 0.001954602546050798 72-73 0.003112885536303123 74-75 0.0020993879198323386 76-77 0.0013030683640338654 78-79 0.0025699403846223454 80-81 0.0015926391115969466 82-83 7.601232123530882E-4 84-85 3.9815977789923665E-4 86-87 0.0014116573943700208 88-89 0.004669328304454684 90-91 1.4478537378154058E-4 92-93 8.687122426892436E-4 94-95 8.687122426892436E-4 96-97 7.239268689077029E-5 98-99 5.429451516807772E-4 100-101 0.0013030683640338654 102-103 0.0015202464247061763 104-105 9.77301273025399E-4 106-107 0.00640675278983317 108-109 0.0022803696370592643 110-111 0.0016288354550423316 112-113 0.002642333071513116 114-115 0.003185278223193893 116-117 0.0021355842632777237 118-119 0.04466628781160527 120-121 0.01216197139764941 122-123 0.005031291738908535 124-125 0.0034386526273115893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1381355.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.0180621813851 #Duplication Level Percentage of deduplicated Percentage of total 1 87.7371531575625 65.81871211191732 2 8.193513679450126 12.29323037378038 3 1.9347432902642705 4.354220773621879 4 0.7195948148147414 2.1593043425269824 5 0.3664046171422563 1.3743482176162192 6 0.20894318413531257 0.9404707667903683 7 0.13232198919165655 0.694857744920098 8 0.10812396961588314 0.6489000540754012 9 0.06964552071781517 0.47022048034776026 >10 0.46766439304062724 6.807708372229119 >50 0.0451596614787186 2.339849333506686 >100 0.01673172258616281 2.098177428667841 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8098171722692573E-4 0.0 22-23 0.0 0.0 0.0 3.981597778992366E-4 0.0 24-25 0.0 0.0 0.0 6.877305254623178E-4 0.0 26-27 0.0 0.0 0.0 9.049085861346286E-4 0.0 28-29 0.0 0.0 0.0 0.0028233147887400415 0.0 30-31 0.0 0.0 0.0 0.010786510346724773 0.0 32-33 0.0 0.0 0.0 0.028920878412862734 0.0 34-35 0.0 0.0 0.0 0.05632151040101929 0.0 36-37 0.0 0.0 0.0 0.10171172508153226 0.0 38-39 1.4478537378154058E-4 0.0 0.0 0.1713534898704533 0.0 40-41 1.4478537378154058E-4 0.0 0.0 0.244723478034249 0.0 42-43 1.4478537378154058E-4 0.0 0.0 0.3171523612684647 0.0 44-45 1.4478537378154058E-4 0.0 0.0 0.40883769921562524 0.0 46-47 1.4478537378154058E-4 0.0 0.0 0.5179696747034614 0.0 48-49 1.4478537378154058E-4 0.0 0.0 0.6392998179323925 0.0 50-51 1.4478537378154058E-4 0.0 0.0 0.7680502115676274 0.0 52-53 1.4478537378154058E-4 0.0 0.0 0.89046624509992 0.0 54-55 1.4478537378154058E-4 0.0 0.0 1.0213160266549872 0.0 56-57 1.4478537378154058E-4 0.0 0.0 1.177467052278379 0.0 58-59 1.4478537378154058E-4 0.0 0.0 1.3502683958866475 0.0 60-61 1.4478537378154058E-4 0.0 0.0 1.537801651277188 0.0 62-63 1.8098171722692573E-4 0.0 0.0 1.7237784638995768 0.0 64-65 2.1717806067231087E-4 0.0 0.0 1.9114927010073441 0.0 66-67 2.8957074756308116E-4 0.0 0.0 2.134100213196463 0.0 68-69 2.8957074756308116E-4 0.0 0.0 2.3918543748710506 0.0 70-71 3.257670910084663E-4 0.0 0.0 2.6692631510364824 0.0 72-73 3.6196343445385145E-4 0.0 0.0 2.9589062912864543 0.0 74-75 3.6196343445385145E-4 0.0 0.0 3.2395727383619706 0.0 76-77 3.6196343445385145E-4 0.0 0.0 3.5527073055079974 0.0 78-79 3.6196343445385145E-4 0.0 0.0 3.900952325796048 0.0 80-81 5.06748808235392E-4 0.0 0.0 4.2775028866583895 0.0 82-83 5.06748808235392E-4 0.0 0.0 4.660243022249892 0.0 84-85 5.429451516807772E-4 0.0 0.0 5.045191134791564 0.0 86-87 5.791414951261623E-4 0.0 0.0 5.434989557354916 0.0 88-89 5.791414951261623E-4 0.0 0.0 5.871698441023488 0.0 90-91 5.791414951261623E-4 0.0 0.0 6.3464496816531595 0.0 92-93 5.791414951261623E-4 0.0 0.0 6.839371486692414 0.0 94-95 5.791414951261623E-4 0.0 0.0 7.326067520659063 0.0 96-97 5.791414951261623E-4 0.0 0.0 7.829703443358152 0.0 98-99 5.791414951261623E-4 0.0 0.0 8.353355943982539 0.0 100-101 5.791414951261623E-4 0.0 0.0 8.937601123534499 0.0 102-103 5.791414951261623E-4 0.0 0.0 9.55786166481462 0.0 104-105 7.239268689077029E-4 0.0 0.0 10.168493978738269 0.0 106-107 7.963195557984732E-4 0.0 0.0 10.753137318068129 0.0 108-109 7.963195557984732E-4 0.0 0.0 11.372311969044887 0.0 110-111 7.963195557984732E-4 0.0 0.0 12.051246783049976 0.0 112-113 7.963195557984732E-4 0.0 0.0 12.758559530316248 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1855 0.0 49.417168 1 GGTATCA 1210 0.0 49.194397 1 ATCAACG 2115 0.0 40.80039 3 CAACGCA 2160 0.0 39.674862 5 TCAACGC 2220 0.0 38.870644 4 AACGCAG 2290 0.0 37.938213 6 TATCAAC 2355 0.0 36.642387 2 GTACATG 5320 0.0 36.58787 1 ACATGGG 5330 0.0 35.17143 3 TACATGG 5655 0.0 33.781513 2 ACGCAGA 2780 0.0 30.604681 7 CGCAGAG 2840 0.0 29.74753 8 CATGGGG 3280 0.0 29.030386 4 GGTAAGG 675 0.0 26.44991 3 CATGGGA 2740 0.0 26.280973 4 TAAGGTG 690 0.0 25.874908 5 GTAAGGT 680 0.0 24.505062 4 GAGTACA 4125 0.0 24.098696 1 GCAGAGT 3585 0.0 23.565685 9 ATGGGGA 2080 0.0 23.175459 5 >>END_MODULE