FastQCFastQC Report
Thu 26 May 2016
SRR1364378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381355
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.17671054870037028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.1700504215064194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.068.572641
GTATCAA37650.052.985991
ATCAACG46800.041.849443
CAACGCA48000.040.6791845
TCAACGC48550.040.218354
AACGCAG49700.039.6284266
TATCAAC49600.039.6069952
GTGGTAT10800.034.7374531
TGGTATC10800.033.6237142
ACGCAGA58300.033.5761647
CGCAGAG59900.032.6793068
GTACATG47450.032.0025941
ACATGGG47750.029.9210933
TACATGG51050.029.1530422
GCAGAGT68350.028.203979
GAGTACT42950.025.83565112-13
CAGAGTA64000.025.7050810-11
AGAGTAC62800.024.82249310-11
GTACTTT45000.024.39425814-15
CATGGGG30000.024.2090764
Produced by FastQC (version 0.11.5)