FastQCFastQC Report
Thu 26 May 2016
SRR1364377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740675
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.048.6004871
GTATCAA26850.047.6792681
ATCAACG29950.041.932063
CAACGCA30300.041.2512665
TCAACGC30500.040.980774
AACGCAG32350.038.633876
TATCAAC34200.037.0703432
GTACATG77100.036.1432231
TACATGG79850.034.5127222
ACATGGG79200.033.66773
ACGCAGA40100.031.1573737
CGCAGAG40900.030.40168
CATGGGG36050.028.8930534
CATGGGA53350.026.7754924
TAATGCG900.00951492926.4531925
GCAGAGT49650.024.2050279
GAGTACA58800.024.1009981
ATGGGAG25300.023.7608225
AGTACAT60700.021.8670042
ATGGGGA22650.021.5479645
Produced by FastQC (version 0.11.5)