FastQCFastQC Report
Thu 26 May 2016
SRR1364377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740675
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.19268387263561548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32420.1862495870854697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29500.067.014181
GTATCAA49250.053.1982231
ATCAACG60050.043.0281333
TCAACGC61150.042.2541164
CAACGCA62100.041.607725
AACGCAG63200.040.861226
TATCAAC63900.040.6220132
GTACATG78600.034.545621
ACGCAGA75250.034.3150377
CGCAGAG77300.033.4058
TACATGG80050.033.1702962
ACATGGG80350.032.1573033
GCAGAGT86150.029.4208749
GAGTACT46300.026.98606512-13
CATGGGG37900.026.3903924
CATGGGA52200.025.8899464
CAGAGTA85600.025.75228110-11
TGGTATC15550.024.5033442
GTACTTT51050.024.06719814-15
AGAGTAC85250.023.72934510-11
Produced by FastQC (version 0.11.5)