FastQCFastQC Report
Thu 26 May 2016
SRR1364376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546862
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.049.8571971
GTATCAA18800.040.2199551
CAACGCA19000.037.2836845
ATCAACG19250.036.7994843
TCAACGC20250.034.9822244
AACGCAG21150.034.0532346
GTACATG47200.031.5350671
TATCAAC23500.030.3985522
ACATGGG46950.029.6691953
TACATGG49550.028.954292
CGCAGAG25400.028.1064668
GTGTAAG8350.027.8082621
ACGCAGA25900.027.564767
TATACCG1150.001002158625.881995
GAGTACA38000.024.7553581
TAAGGTG11150.024.5588745
GTAAGGT9600.024.1834834
GGTAAGG10300.023.1178933
CATGGGG34200.022.2797014
CCGTTGC2153.4275963E-622.1365649
Produced by FastQC (version 0.11.5)