FastQCFastQC Report
Thu 26 May 2016
SRR1364376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546862
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.16956910183325985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.16504381127728265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.069.1855851
GTATCAA38150.050.2599641
ATCAACG46100.040.8096353
CAACGCA47000.040.154855
TCAACGC47100.039.943194
TATCAAC48850.038.7560122
AACGCAG49150.038.503276
TGGTATC11650.034.2393462
ACGCAGA58000.032.620827
CGCAGAG58950.032.196058
GTGGTAT13500.030.8762931
GTACATG47950.029.8046151
CATACCG1403.0809333E-629.7677885
ACATGGG45850.029.6054783
TACATGG47900.029.332772
GTAAGGT8850.027.5814574
TAAGGTG9550.027.4300065
GCAGAGT69150.026.9307149
GAGTACT44650.025.11803212-13
CAGAGTA65500.025.0705310-11
Produced by FastQC (version 0.11.5)