FastQCFastQC Report
Thu 26 May 2016
SRR1364375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464407
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.054.6574751
GTATCAA25200.052.213531
ATCAACG27600.046.3646743
TCAACGC27950.045.7840774
CAACGCA28850.044.3558045
AACGCAG29450.043.649756
TATCAAC34150.038.3433272
GTACATG69100.037.5664181
ACATGGG72550.034.2923053
ACGCAGA37750.034.042127
TACATGG76050.033.809782
CGCAGAG38500.033.0698978
CATGGGA51850.029.8457434
CATGGGG27800.026.3340784
GCAGAGT48250.026.0174629
ATGGGAG23500.024.5675415
ATGGGAT23150.024.4247675
TAAGGTG8550.024.3646245
GTAAGGT8500.023.1074924
AGAGTAC43550.021.99467510-11
Produced by FastQC (version 0.11.5)