##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364375_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1464407 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.100559475610265 31.0 31.0 34.0 28.0 34.0 2 31.183576696915544 33.0 31.0 34.0 28.0 34.0 3 31.35716027033468 33.0 31.0 34.0 28.0 34.0 4 34.773505589634574 37.0 35.0 37.0 32.0 37.0 5 34.88637107033769 37.0 35.0 37.0 32.0 37.0 6 34.93583682678381 37.0 35.0 37.0 32.0 37.0 7 34.84464633124534 37.0 35.0 37.0 32.0 37.0 8 34.82904957433282 37.0 35.0 37.0 32.0 37.0 9 36.386977117700205 39.0 37.0 39.0 32.0 39.0 10-11 36.31030649266222 39.0 37.0 39.0 32.0 39.0 12-13 36.33910722906951 39.0 37.0 39.0 32.0 39.0 14-15 37.67198394981723 40.0 38.0 41.0 32.0 41.0 16-17 37.69350563060679 40.0 38.0 41.0 32.0 41.0 18-19 37.69293509249819 40.0 38.0 41.0 32.0 41.0 20-21 37.720586558245074 40.0 38.0 41.0 32.0 41.0 22-23 37.689099751640086 40.0 38.0 41.0 32.0 41.0 24-25 37.702088285565424 40.0 38.0 41.0 32.0 41.0 26-27 37.663948956813236 40.0 38.0 41.0 32.0 41.0 28-29 37.66817728951036 40.0 38.0 41.0 32.0 41.0 30-31 37.57249180043526 40.0 38.0 41.0 31.0 41.0 32-33 37.535510278221835 40.0 38.0 41.0 31.0 41.0 34-35 37.53609174225471 40.0 38.0 41.0 31.0 41.0 36-37 37.47670558799568 40.0 38.0 41.0 31.0 41.0 38-39 37.39303007975242 40.0 38.0 41.0 31.0 41.0 40-41 37.32852000844028 40.0 37.5 41.0 31.0 41.0 42-43 37.20352231312743 40.0 37.0 41.0 30.0 41.0 44-45 37.0799436222307 40.0 37.0 41.0 30.0 41.0 46-47 36.97686947685992 40.0 37.0 41.0 30.0 41.0 48-49 36.83801736812239 40.0 36.5 41.0 30.0 41.0 50-51 36.72309610647859 40.0 36.0 41.0 30.0 41.0 52-53 36.57138998925845 40.0 36.0 41.0 30.0 41.0 54-55 36.38324523168764 39.5 35.5 41.0 30.0 41.0 56-57 36.19111558467011 39.0 35.0 41.0 29.0 41.0 58-59 35.979756652351426 39.0 35.0 41.0 29.0 41.0 60-61 35.73667532318542 39.0 35.0 41.0 29.0 41.0 62-63 35.47077178680517 38.0 35.0 40.0 28.5 41.0 64-65 35.138032664416386 37.5 34.0 40.0 28.0 41.0 66-67 34.82863097485877 37.0 34.0 40.0 28.0 41.0 68-69 34.50234668367469 36.5 34.0 39.5 28.0 41.0 70-71 34.13806544218923 36.0 34.0 39.0 27.0 41.0 72-73 33.76943739001521 35.5 34.0 39.0 26.5 40.5 74-75 33.41443191681002 35.0 33.0 37.5 26.0 40.0 76-77 33.171881177841954 35.0 33.0 37.0 26.5 39.0 78-79 32.99441685269191 35.0 33.0 37.0 26.5 39.0 80-81 32.71469680218682 35.0 33.0 36.0 26.0 39.0 82-83 32.4194172111988 35.0 33.0 36.0 26.0 37.0 84-85 32.161654854149155 35.0 33.0 35.5 26.0 37.0 86-87 31.94594125813384 35.0 33.0 35.0 26.0 36.5 88-89 31.678134562317716 35.0 33.0 35.0 25.0 36.0 90-91 31.5479487601466 35.0 33.0 35.0 25.0 36.0 92-93 31.4000339386523 35.0 33.0 35.0 24.5 36.0 94-95 31.288856171815624 35.0 33.0 35.0 24.0 35.5 96-97 31.17676165164466 35.0 32.0 35.0 24.0 35.0 98-99 31.04451289839505 35.0 32.0 35.0 23.0 35.0 100-101 30.954617124883995 35.0 32.0 35.0 23.0 35.0 102-103 29.937946554475634 33.5 30.0 34.5 19.5 35.0 104-105 30.629966259380076 34.5 31.5 35.0 20.0 35.0 106-107 30.953912402767813 35.0 32.5 35.0 23.0 35.0 108-109 30.979596860708803 35.0 33.0 35.0 21.5 35.0 110-111 30.90337146708531 35.0 33.0 35.0 20.0 35.0 112-113 30.80572614034213 35.0 33.0 35.0 19.5 35.0 114-115 30.695386938194098 35.0 33.0 35.0 18.5 35.0 116-117 30.55366438428661 35.0 32.0 35.0 16.0 35.0 118-119 30.373457652141788 35.0 32.0 35.0 4.5 35.0 120-121 30.192193153952417 35.0 32.0 35.0 2.0 35.0 122-123 30.00797626616098 35.0 32.0 35.0 2.0 35.0 124-125 29.183740585779773 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.287624568068896 1101 2 0.2632109181307918 1101 3 0.25661309363942664 1101 4 0.3533743216140053 1101 5 0.31207729541051066 1101 6 0.3250419890363716 1101 7 0.4193220371881594 1101 8 0.35543904537212967 1101 9 0.45670525033947484 1101 10-11 0.5661917547071198 1101 12-13 0.6062782587091746 1101 14-15 0.7649872383870786 1101 16-17 0.579420436901728 1101 18-19 0.4124392147333751 1101 20-21 0.5997665307132536 1101 22-23 0.7475950157049169 1101 24-25 0.712025273792527 1101 26-27 0.8342677067101292 1101 28-29 0.736748752971522 1101 30-31 0.7798235469252717 1101 32-33 0.6656981959970807 1101 34-35 0.7334732861632105 1101 36-37 0.6622102660618623 1101 38-39 0.7508263373097819 1101 40-41 0.6758973296284339 1101 42-43 0.7213100117957083 1101 44-45 0.7524768630260112 1101 46-47 0.6940822076800259 1101 48-49 0.6169115722667726 1101 50-51 0.5112871153655547 1101 52-53 0.4877769734849906 1101 54-55 0.5726462304797479 1101 56-57 0.5429113599156423 1101 58-59 0.5370554691909817 1101 60-61 0.5793599318696891 1101 62-63 0.5088754175389667 1101 64-65 0.6044928244654386 1101 66-67 0.6045816737336551 1101 68-69 0.6985680234737686 1101 70-71 0.5660261322170115 1101 72-73 0.5595298333294565 1101 74-75 0.5357835860434221 1101 76-77 0.6007182294181348 1101 78-79 0.6394861095311981 1101 80-81 0.584853869791651 1101 82-83 0.6044456598926828 1101 84-85 0.5655781671632596 1101 86-87 0.5597659068555885 1101 88-89 0.6182380911470418 1101 90-91 0.4805024459181624 1101 92-93 0.4049797456055586 1101 94-95 0.41503288469803934 1101 96-97 0.3821208212201306 1101 98-99 0.40305826995647465 1101 100-101 0.4104500800452726 1101 102-103 0.15973197634115266 1101 104-105 0.3800874610981033 1101 106-107 0.45114586191611394 1101 108-109 0.40061969551302923 1101 110-111 0.4306651415583005 1101 112-113 0.5126087505461747 1101 114-115 0.38838540033382074 1101 116-117 0.3759364345174774 1101 118-119 0.4486244100156469 1101 120-121 0.31937587583109206 1101 122-123 0.36337424076927505 1101 124-125 0.3153188881609523 1102 1 0.18700144537204721 1102 2 0.22240979332068278 1102 3 0.38068332073994426 1102 4 0.4490232096065938 1102 5 0.4788946140576158 1102 6 0.4107565482395472 1102 7 0.40215249747401316 1102 8 0.3930759347230861 1102 9 0.4570015506966669 1102 10-11 0.523695931084589 1102 12-13 0.4471462577702354 1102 14-15 0.4928311181204563 1102 16-17 0.5733801082911398 1102 18-19 0.46367437605717754 1102 20-21 0.5657824895984263 1102 22-23 0.6601089632501882 1102 24-25 0.5825575723806224 1102 26-27 0.661300415267398 1102 28-29 0.5727850112592918 1102 30-31 0.6934331749029852 1102 32-33 0.7018693557910822 1102 34-35 0.6359693664923682 1102 36-37 0.46813886202674126 1102 38-39 0.5300820273317441 1102 40-41 0.44884880533256677 1102 42-43 0.4027069613813552 1102 44-45 0.4298129531274313 1102 46-47 0.40902483918833354 1102 48-49 0.433025656171111 1102 50-51 0.43063783469884953 1102 52-53 0.5350822392122367 1102 54-55 0.49996442725930024 1102 56-57 0.5095078456019664 1102 58-59 0.3262652848691019 1102 60-61 0.3133595494203334 1102 62-63 0.3092057424011969 1102 64-65 0.2967345411627065 1102 66-67 0.39293010816045637 1102 68-69 0.35160987731328674 1102 70-71 0.2966936760371581 1102 72-73 0.2701900327295874 1102 74-75 0.22457074949397082 1102 76-77 0.2754719161494634 1102 78-79 0.4340850239232381 1102 80-81 0.34022213828590253 1102 82-83 0.31210195669948604 1102 84-85 0.386367901160817 1102 86-87 0.31832168844350406 1102 88-89 0.3815050919239056 1102 90-91 0.30466656672952297 1102 92-93 0.28105821255360297 1102 94-95 0.18987409098422248 1102 96-97 0.22733390620938465 1102 98-99 0.17796822140629232 1102 100-101 0.08886314145232177 1102 102-103 0.004963879231208779 1102 104-105 0.12836671091432805 1102 106-107 0.1760170571921904 1102 108-109 0.212605915153393 1102 110-111 0.18113063986175248 1102 112-113 0.2391679006471037 1102 114-115 0.11956287663359788 1102 116-117 0.07128190833372372 1102 118-119 0.13808238268161332 1102 120-121 0.0350582792664369 1102 122-123 0.010942478502229847 1102 124-125 -0.0622341190374911 1103 1 0.10754726234868173 1103 2 0.09309836896800405 1103 3 0.16047429286478732 1103 4 0.1696843880434571 1103 5 0.16148241978764588 1103 6 0.23733731955682202 1103 7 0.09407399077311851 1103 8 0.2027145256314853 1103 9 0.3498660716613813 1103 10-11 0.3343576845106 1103 12-13 0.27144207219009786 1103 14-15 0.3661812344705382 1103 16-17 0.2086942603196391 1103 18-19 0.3045518095055897 1103 20-21 0.37117434243393177 1103 22-23 0.3737620920661655 1103 24-25 0.39141651890503937 1103 26-27 0.39896411388399144 1103 28-29 0.35327801559579797 1103 30-31 0.5547943128633364 1103 32-33 0.5207039095938129 1103 34-35 0.5258253078462047 1103 36-37 0.4194705755421424 1103 38-39 0.3898253549007933 1103 40-41 0.4256493565948958 1103 42-43 0.31536974524248507 1103 44-45 0.35103318920024407 1103 46-47 0.3215220579907978 1103 48-49 0.31785146513880846 1103 50-51 0.27781833106675435 1103 52-53 0.22816427880103873 1103 54-55 0.16889499061457514 1103 56-57 0.10437446516345261 1103 58-59 0.1430988536904252 1103 60-61 0.00103691823360208 1103 62-63 0.06206154080318527 1103 64-65 0.17059601150315018 1103 66-67 0.0880846086551017 1103 68-69 0.102080152533091 1103 70-71 0.06169538168680333 1103 72-73 0.20792495727533122 1103 74-75 0.018344866936750748 1103 76-77 -0.05094090379051863 1103 78-79 0.10102517027956992 1103 80-81 0.12275101330939009 1103 82-83 0.07967838650774439 1103 84-85 0.09359262851254613 1103 86-87 0.08211034879377621 1103 88-89 0.19484759023586662 1103 90-91 0.12977611579939108 1103 92-93 0.023363528157876345 1103 94-95 0.03641221310600784 1103 96-97 0.03803122714628415 1103 98-99 0.20592360315704283 1103 100-101 0.02172883966756345 1103 102-103 -0.2681118786579013 1103 104-105 -0.041591105790494964 1103 106-107 -0.03221719657075184 1103 108-109 0.16873662905129194 1103 110-111 0.1791740858603852 1103 112-113 0.09060899846275916 1103 114-115 -0.18250835702267665 1103 116-117 -0.19016009173763848 1103 118-119 -0.21819737385934346 1103 120-121 -0.2630835966936296 1103 122-123 -0.25261459530751296 1103 124-125 -0.35971168934695896 1104 1 0.24400353312987022 1104 2 0.3129831618099388 1104 3 0.26495112067515336 1104 4 0.38759543522841966 1104 5 0.36481394741494455 1104 6 0.240021828467043 1104 7 0.32117359102716136 1104 8 0.46909056702574503 1104 9 0.4985650293331432 1104 10-11 0.6226215682106826 1104 12-13 0.4438599514817625 1104 14-15 0.5859030467767354 1104 16-17 0.6233084541030323 1104 18-19 0.5759611007971017 1104 20-21 0.6359514000048136 1104 22-23 0.4972173961719619 1104 24-25 0.5483835865629771 1104 26-27 0.5837420120421726 1104 28-29 0.41512352499169225 1104 30-31 0.46291928951369243 1104 32-33 0.45404440348554687 1104 34-35 0.5320317607546414 1104 36-37 0.47425106551113316 1104 38-39 0.3744055982975141 1104 40-41 0.4792836123949442 1104 42-43 0.41972398740633565 1104 44-45 0.419172362435944 1104 46-47 0.3603803069540703 1104 48-49 0.273729622011075 1104 50-51 0.2990558857049308 1104 52-53 0.16473391211818011 1104 54-55 0.23582301766280978 1104 56-57 0.21616977281840377 1104 58-59 0.22517146175258773 1104 60-61 0.06452682651619313 1104 62-63 0.2079385768923956 1104 64-65 0.2885676510219639 1104 66-67 0.33077476817989293 1104 68-69 0.129820817344239 1104 70-71 -0.019825707714325347 1104 72-73 0.11663285786186606 1104 74-75 0.15438282879276954 1104 76-77 0.20339173994990745 1104 78-79 0.2712034681837068 1104 80-81 0.31655927067853895 1104 82-83 0.2974081236841144 1104 84-85 0.23403503427337569 1104 86-87 0.23510564150495128 1104 88-89 0.2628710659709128 1104 90-91 0.2616305214107797 1104 92-93 0.09860122756386147 1104 94-95 0.13518507241037625 1104 96-97 0.08221167788786587 1104 98-99 0.04025820826781157 1104 100-101 0.11464233856546002 1104 102-103 -0.007850409311320306 1104 104-105 0.10456087239159828 1104 106-107 0.1408962687036528 1104 108-109 0.09916822392557734 1104 110-111 -0.01569891540983548 1104 112-113 0.08069392112873075 1104 114-115 -0.08481635287578015 1104 116-117 -0.09414657696198248 1104 118-119 -0.07074750555135267 1104 120-121 -0.08971258481333066 1104 122-123 -0.07261181669952776 1104 124-125 -0.1705665899708606 1105 1 0.16396690309323958 1105 2 0.21465440723118334 1105 3 0.10251980199383226 1105 4 0.28393243156541814 1105 5 0.29869673129773133 1105 6 0.2994769566721729 1105 7 0.29104538589896123 1105 8 0.4112608966960707 1105 9 0.4119349927031024 1105 10-11 0.30676763048174394 1105 12-13 0.4257166364634415 1105 14-15 0.4988380284617122 1105 16-17 0.4261129412825184 1105 18-19 0.4159336282696273 1105 20-21 0.3989092754626924 1105 22-23 0.4964046672342377 1105 24-25 0.4251235133028999 1105 26-27 0.33012479592495225 1105 28-29 0.31294861656678563 1105 30-31 0.37672423456864124 1105 32-33 0.3774876269287688 1105 34-35 0.27540401350189114 1105 36-37 0.19470665159172285 1105 38-39 0.26065788767480313 1105 40-41 0.22105330103963183 1105 42-43 0.20818552586788286 1105 44-45 0.15591686793045056 1105 46-47 0.21612664395040326 1105 48-49 0.15464766230411442 1105 50-51 0.07594462196866658 1105 52-53 0.18111440662367073 1105 54-55 0.08389994073974094 1105 56-57 0.1266093332579672 1105 58-59 0.12407256065367989 1105 60-61 0.079167394281761 1105 62-63 0.1026386684674847 1105 64-65 0.1734005264798384 1105 66-67 0.02225828466340829 1105 68-69 0.04523977338820373 1105 70-71 0.09459736920874917 1105 72-73 0.09972580657981922 1105 74-75 0.06996204124698124 1105 76-77 0.04303230771547817 1105 78-79 0.1367254462056806 1105 80-81 0.1461494721437404 1105 82-83 0.040665907566904025 1105 84-85 0.04345582181916541 1105 86-87 0.08048109938040682 1105 88-89 0.09449881322366238 1105 90-91 0.10264471554997101 1105 92-93 0.0739217403843746 1105 94-95 0.09687234347264351 1105 96-97 -0.15399482394363417 1105 98-99 -0.10348034484573887 1105 100-101 -0.17032789952978078 1105 102-103 -0.3694117646226722 1105 104-105 -0.17593363310290755 1105 106-107 -0.2257131635307772 1105 108-109 -0.20032811307076415 1105 110-111 -0.12161695570287634 1105 112-113 -0.1311536063437977 1105 114-115 -0.22406772650215245 1105 116-117 -0.3462299102953139 1105 118-119 -0.19514082056966942 1105 120-121 -0.31591450789025544 1105 122-123 -0.4130078789705891 1105 124-125 -0.6825743738536474 1106 1 0.36560792376410944 1106 2 0.3178571746130565 1106 3 0.3813696834556559 1106 4 0.336507344214958 1106 5 0.32928616673791566 1106 6 0.2070501049169593 1106 7 0.1866373561177923 1106 8 0.24250365890897996 1106 9 0.452570352368312 1106 10-11 0.3735127634582227 1106 12-13 0.37279971507637555 1106 14-15 0.4252850267370718 1106 16-17 0.39299835686195905 1106 18-19 0.44153489267686297 1106 20-21 0.26143977978424005 1106 22-23 0.37076041099296475 1106 24-25 0.3530199328038037 1106 26-27 0.405853482661108 1106 28-29 0.39015157529959055 1106 30-31 0.5305172698915186 1106 32-33 0.4744832929542824 1106 34-35 0.41874667829977597 1106 36-37 0.41283491636178127 1106 38-39 0.34473456969924854 1106 40-41 0.40343373155439366 1106 42-43 0.4040419082914255 1106 44-45 0.28054969778715844 1106 46-47 0.3762252518102116 1106 48-49 0.2859964130558552 1106 50-51 0.28139278077502894 1106 52-53 0.2354838555826788 1106 54-55 0.14376016757757526 1106 56-57 0.20408596110772947 1106 58-59 0.20102514786746184 1106 60-61 0.12292680651132315 1106 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70-71 -0.9929973009998037 2216 72-73 -0.9257032708330897 2216 74-75 -0.7941128686581962 2216 76-77 -0.88346270107197 2216 78-79 -1.0674664860354781 2216 80-81 -0.950630966986072 2216 82-83 -0.9218433104988932 2216 84-85 -0.8467576874244713 2216 86-87 -0.8637342951931757 2216 88-89 -0.9376688721402004 2216 90-91 -1.0085238122239915 2216 92-93 -0.8855655333020138 2216 94-95 -0.7683055382831192 2216 96-97 -0.7636445576290036 2216 98-99 -0.8125538148486733 2216 100-101 -0.7461951042085175 2216 102-103 -0.7534959329647393 2216 104-105 -0.8964960427725899 2216 106-107 -0.6868934998488818 2216 108-109 -0.6194288201031384 2216 110-111 -0.5399516212822135 2216 112-113 -0.6521359762883314 2216 114-115 -0.5959091932646352 2216 116-117 -0.6770398822239478 2216 118-119 -0.68682572151247 2216 120-121 -0.569807290346592 2216 122-123 -0.6483227263813234 2216 124-125 -0.5382453683012756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19971.0 3 3817.0 4 2495.0 5 2039.0 6 2380.0 7 2832.0 8 3027.0 9 3312.0 10 3782.0 11 4199.0 12 4729.0 13 4609.0 14 4566.0 15 4407.0 16 4301.0 17 3927.0 18 3741.0 19 3657.0 20 3633.0 21 3874.0 22 4584.0 23 6148.0 24 8966.0 25 12612.0 26 15230.0 27 15722.0 28 18205.0 29 22504.0 30 26718.0 31 32757.0 32 41751.0 33 56673.0 34 79974.0 35 121120.0 36 237707.0 37 466214.0 38 207780.0 39 444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2395756120389 18.729568814453238 13.136624640052469 23.894230933455393 2 15.253690937823968 20.36391028381533 39.99892095813454 24.38347782022616 3 18.47348584953656 25.59511980928304 29.374017068102372 26.557377273078032 4 12.315461678719961 16.829881242616075 37.87172290399022 32.98293417467374 5 13.194108144844286 38.90448104750163 34.63367154922459 13.267739258429497 6 31.578160039399528 37.274082857859106 17.887849330966265 13.259907771775104 7 28.610851042471204 32.778823743117286 21.27630079277116 17.33402442164035 8 26.327721733097427 35.500922899166696 20.222998114595192 17.94835725314069 9 26.754037641174893 16.47547437290316 19.55153178044082 37.21895620548112 10-11 24.473489951905446 27.167276583627363 28.11134472861711 20.247888735850076 12-13 25.194747726790833 24.557965766738402 27.97637521856303 22.270911287907733 14-15 22.3301163139281 26.093878785768087 25.18818300203701 26.3878218982668 16-17 22.48882651998593 29.510176488061706 25.953066262406914 22.04793072954545 18-19 22.64341309718753 28.403496025009456 27.154081586663807 21.799009291139207 20-21 23.298133647271555 27.572286939354974 27.05699303540614 22.072586377967326 22-23 23.104915505047437 27.42605027154336 26.95316261121396 22.515871612195244 24-25 22.880797483213343 27.847415370180556 26.83161170357694 22.44017544302916 26-27 22.59771804853099 27.976593898748604 26.707306621906717 22.718381430813686 28-29 22.665615497394498 27.88717024913258 26.868938221156636 22.578276032316285 30-31 22.60777169680351 27.669252361114953 26.944076547208475 22.77889939487307 32-33 22.368482151758876 27.8658455806534 26.74709540139934 23.018576866188383 34-35 23.060893923036 27.759579185727386 26.506334680643732 22.673192210592877 36-37 23.151715832807238 27.32713036109192 26.490886574790927 23.030267231309907 38-39 22.601762424085983 27.625514415937957 26.747887450419235 23.024835709556825 40-41 22.866723595692832 27.337370718927556 27.054079957170067 22.741825728209538 42-43 22.885197406159005 27.25165119722042 26.778895268480024 23.084256128140552 44-45 22.60435221880667 27.529953813202113 26.887155959230512 22.978538008760708 46-47 22.961930311016783 27.637817778055478 26.417869628412603 22.982382282515136 48-49 22.739648198895527 27.589256265505423 26.508204344830368 23.162891190768686 50-51 22.747159771839385 27.448209411727753 26.762197940872994 23.042432875559868 52-53 23.051199545475654 27.28914741175153 26.639319633867526 23.02033340890529 54-55 22.62060770783041 27.361705324187096 26.8880155426792 23.129671425303293 56-57 22.689404441118104 27.596376961034032 26.74651018911422 22.967708408733646 58-59 22.705977915164485 27.126154526926925 26.84197641557267 23.325891142335912 60-61 22.543594765350356 27.312079519474242 27.10434882367884 23.039976891496565 62-63 22.53596531541652 27.394552160377273 27.1481025978611 22.92137992634511 64-65 22.426262711794962 27.303009858043087 26.871532681229294 23.39919474893266 66-67 22.404640635195307 27.579260274992258 26.613792908000956 23.40230618181148 68-69 22.459804856196172 27.199909038512494 27.04509695729727 23.295189147994066 70-71 22.452941704700383 27.093060854060813 26.92360353063975 23.530393910599056 72-73 22.455822389514147 27.127036622499865 26.775999011180897 23.64114197680509 74-75 22.323907377771526 27.414804300937224 26.79095602673762 23.47033229455363 76-77 22.434639144845004 27.108463970821973 27.031230420595065 23.42566646373796 78-79 22.510415734810373 27.05950199778608 27.017880031767906 23.41220223563564 80-81 22.52027548643925 27.431906016936047 26.76347138043344 23.284347116191263 82-83 22.569151970958785 27.188411101736786 26.877874681226427 23.364562246078005 84-85 22.459665179935655 27.09409358176753 26.915829263687446 23.53041197460937 86-87 22.569564779453316 27.055376961804463 26.95809980042646 23.41695845831576 88-89 22.499853173567963 27.138885512260348 26.914172779881913 23.447088534289776 90-91 22.39475033785191 27.142670815809076 26.99144836892131 23.4711304774177 92-93 22.50614079474893 26.818832726594817 27.13316220370708 23.541864274949177 94-95 22.232002562160737 26.62250262737565 27.284382769878768 23.861112040584846 96-97 22.536518344360594 26.53815450822555 27.24608901523558 23.679238132178277 98-99 22.366605139467342 26.71297937097487 26.984320465885865 23.936095023671925 100-101 22.102843610148536 26.57303714144163 27.39088704944077 23.93323219896906 102-103 22.308166383556266 26.351836518681022 27.33893863475344 24.00105846300928 104-105 22.08349594320383 26.590922050035015 27.21698285537128 24.108599151389875 106-107 22.185408980958794 26.582908670229692 27.205152229942264 24.026530118869246 108-109 22.31260565489045 26.486287527439117 26.93914175877343 24.261965058897 110-111 22.157337146540797 26.845656603732355 27.032322123158952 23.964684126567896 112-113 22.025460034615485 26.888017298865353 27.32175227153455 23.764770394984613 114-115 21.98173546540214 26.88364536783104 27.112105700206506 24.02251346656032 116-117 21.891613202812103 26.869470801735883 27.192029418493085 24.04688657695893 118-119 21.824337341824904 26.996070302775244 26.874189568266193 24.305402787133655 120-121 21.79569352355306 27.265438335951874 26.967569788077267 23.971298352417797 122-123 21.76252722642036 27.297100640185807 26.95524026003515 23.98513187335869 124-125 21.83463070492382 27.197684193418198 26.937060348777734 24.030624752880247 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 768.0 1 603.0 2 386.5 3 309.0 4 243.5 5 185.0 6 176.0 7 186.5 8 183.5 9 169.5 10 162.5 11 159.0 12 154.5 13 161.0 14 179.5 15 201.0 16 213.0 17 250.5 18 310.5 19 404.0 20 539.5 21 724.0 22 1053.5 23 1515.5 24 2074.5 25 3084.0 26 4435.0 27 6166.0 28 8245.0 29 10775.5 30 14116.0 31 17757.0 32 22444.0 33 26131.5 34 28649.0 35 32275.0 36 37402.5 37 41845.5 38 44084.0 39 45621.5 40 46379.5 41 48481.0 42 50502.0 43 53025.0 44 56755.5 45 59377.0 46 58664.0 47 57885.0 48 57889.0 49 55865.5 50 54609.0 51 52922.5 52 51259.0 53 50700.5 54 50100.0 55 47000.0 56 42484.0 57 38518.5 58 35488.0 59 31665.5 60 25500.5 61 20172.5 62 16473.5 63 12559.5 64 9167.5 65 7145.5 66 5473.5 67 4384.5 68 3290.5 69 2044.0 70 1354.5 71 924.5 72 660.5 73 519.0 74 393.0 75 287.5 76 190.5 77 101.0 78 54.5 79 41.5 80 29.5 81 20.0 82 13.0 83 7.0 84 7.0 85 8.5 86 12.0 87 8.5 88 3.5 89 3.5 90 3.0 91 2.5 92 2.0 93 1.5 94 1.5 95 3.5 96 3.0 97 1.5 98 1.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044523141449064364 2 0.009901618880543455 3 0.0025949070169700088 4 0.005258101060702387 5 0.02403703342035377 6 0.02936342150781852 7 0.0034143513381184325 8 0.0 9 0.0 10-11 0.0 12-13 8.535878345296081E-4 14-15 5.462962140989492E-4 16-17 1.365740535247373E-4 18-19 6.828702676236865E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7071756690592165E-4 28-29 4.097221605742119E-4 30-31 2.3900459366829033E-4 32-33 3.414351338118433E-4 34-35 5.462962140989492E-4 36-37 1.0243054014355298E-4 38-39 2.3900459366829033E-4 40-41 7.17013781004871E-4 42-43 0.0010243054014355299 44-45 0.001877893235965138 46-47 3.4143513381184327E-5 48-49 0.0 50-51 0.0 52-53 0.0012974535084850044 54-55 0.0035509253916431705 56-57 6.828702676236865E-5 58-59 5.804397274801336E-4 60-61 7.511572943860553E-4 62-63 6.828702676236866E-4 64-65 0.0012974535084850044 66-67 0.0026290505303511935 68-69 0.0034826383648808015 70-71 0.0018096062092027693 72-73 0.0023217589099205345 74-75 0.0019461802627275067 76-77 0.0011950229683414515 78-79 0.0024583329634452716 80-81 0.0010584489148167142 82-83 3.7557864719302763E-4 84-85 5.804397274801336E-4 86-87 0.0023559024233017188 88-89 0.006350693488900285 90-91 5.462962140989492E-4 92-93 0.0013657405352473732 94-95 0.0012291664817226358 96-97 4.097221605742119E-4 98-99 9.560183746731613E-4 100-101 0.00126330999510382 102-103 0.0013315970218661889 104-105 8.535878345296081E-4 106-107 0.007443285917098183 108-109 0.0019461802627275067 110-111 0.001570601615534479 112-113 0.00228761539653935 114-115 0.0026631940437323778 116-117 0.0015023145887721105 118-119 0.046503465225173055 120-121 0.011642938062983856 122-123 0.004541087279697515 124-125 0.0024583329634452716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1464407.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.13930603686006 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5084732991997 56.06700945505926 2 10.033067868898414 13.47226428277116 3 2.94393131016479 5.929605155539448 4 1.2053516532516624 3.2370589411879447 5 0.6333385361032691 2.126095490018716 6 0.3649248028365217 1.4700478810849231 7 0.2256728790233092 1.0606064342275676 8 0.17811755210156058 0.9566951072866435 9 0.12458298386133486 0.7527973572406225 >10 0.6942798648558738 8.786134054894209 >50 0.06205431376137974 2.8460129518463066 >100 0.025785656967403103 3.0303582019764392 >500 2.0963948738803666E-4 0.09458433399176737 >1k 2.0963948738803666E-4 0.17073035287502056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 6.828702676236865E-5 18-19 0.0 0.0 0.0 0.0 6.828702676236865E-5 20-21 0.0 0.0 0.0 3.4143513381184327E-5 6.828702676236865E-5 22-23 0.0 0.0 0.0 1.365740535247373E-4 6.828702676236865E-5 24-25 0.0 0.0 0.0 8.535878345296082E-4 6.828702676236865E-5 26-27 0.0 0.0 0.0 0.0014681710753909262 6.828702676236865E-5 28-29 0.0 0.0 0.0 0.003721642958549092 6.828702676236865E-5 30-31 0.0 0.0 0.0 0.012325808330607542 6.828702676236865E-5 32-33 0.0 0.0 0.0 0.03226562014521919 6.828702676236865E-5 34-35 0.0 0.0 0.0 0.06391665704957707 6.828702676236865E-5 36-37 0.0 0.0 0.0 0.1165659546833633 6.828702676236865E-5 38-39 0.0 0.0 0.0 0.19772508599043848 6.828702676236865E-5 40-41 0.0 0.0 0.0 0.2852690542997951 6.828702676236865E-5 42-43 0.0 0.0 0.0 0.3742129066577803 6.828702676236865E-5 44-45 0.0 0.0 0.0 0.4800236546260705 6.828702676236865E-5 46-47 0.0 0.0 0.0 0.6085739825062295 6.828702676236865E-5 48-49 0.0 0.0 0.0 0.7498939843909513 6.828702676236865E-5 50-51 0.0 0.0 0.0 0.9051786832485778 1.365740535247373E-4 52-53 3.4143513381184327E-5 0.0 0.0 1.0536688229433484 1.365740535247373E-4 54-55 6.828702676236865E-5 0.0 0.0 1.2085437996404005 1.365740535247373E-4 56-57 6.828702676236865E-5 0.0 0.0 1.3996791875482706 1.365740535247373E-4 58-59 6.828702676236865E-5 0.0 0.0 1.604847559455807 1.365740535247373E-4 60-61 6.828702676236865E-5 0.0 0.0 1.8278047018349408 1.365740535247373E-4 62-63 6.828702676236865E-5 0.0 0.0 2.0522300152894655 1.365740535247373E-4 64-65 6.828702676236865E-5 0.0 0.0 2.2734799819995395 1.365740535247373E-4 66-67 6.828702676236865E-5 0.0 0.0 2.5301709155992835 1.365740535247373E-4 68-69 6.828702676236865E-5 0.0 0.0 2.821278510687261 1.365740535247373E-4 70-71 6.828702676236865E-5 0.0 0.0 3.1316771908356076 1.365740535247373E-4 72-73 6.828702676236865E-5 0.0 0.0 3.454026100667369 1.7071756690592165E-4 74-75 6.828702676236865E-5 0.0 0.0 3.770946191871522 2.0486108028710599E-4 76-77 6.828702676236865E-5 0.0 0.0 4.1096839881262515 3.0729162043065895E-4 78-79 6.828702676236865E-5 0.0 0.0 4.496803142842119 3.414351338118433E-4 80-81 6.828702676236865E-5 0.0 0.0 4.917826806345504 3.414351338118433E-4 82-83 6.828702676236865E-5 6.828702676236865E-5 0.0 5.354829634111282 3.414351338118433E-4 84-85 6.828702676236865E-5 6.828702676236865E-5 0.0 5.796919845370857 3.414351338118433E-4 86-87 6.828702676236865E-5 6.828702676236865E-5 0.0 6.245975333360192 3.414351338118433E-4 88-89 6.828702676236865E-5 6.828702676236865E-5 0.0 6.7235747985362 3.414351338118433E-4 90-91 6.828702676236865E-5 6.828702676236865E-5 0.0 7.254574718640378 3.414351338118433E-4 92-93 6.828702676236865E-5 6.828702676236865E-5 0.0 7.807802065955707 4.0972216057421197E-4 94-95 6.828702676236865E-5 6.828702676236865E-5 0.0 8.349181614127767 4.0972216057421197E-4 96-97 6.828702676236865E-5 6.828702676236865E-5 0.0 8.895785119847147 4.0972216057421197E-4 98-99 6.828702676236865E-5 6.828702676236865E-5 0.0 9.459426238743738 4.0972216057421197E-4 100-101 6.828702676236865E-5 6.828702676236865E-5 0.0 10.088042463604722 4.0972216057421197E-4 102-103 6.828702676236865E-5 6.828702676236865E-5 0.0 10.757357756416079 4.0972216057421197E-4 104-105 6.828702676236865E-5 6.828702676236865E-5 0.0 11.422712401675216 4.0972216057421197E-4 106-107 6.828702676236865E-5 6.828702676236865E-5 0.0 12.069049109981037 4.0972216057421197E-4 108-109 6.828702676236865E-5 6.828702676236865E-5 0.0 12.747514864378552 4.0972216057421197E-4 110-111 6.828702676236865E-5 6.828702676236865E-5 0.0 13.464733506463709 4.0972216057421197E-4 112-113 6.828702676236865E-5 6.828702676236865E-5 0.0 14.210905847896111 4.780091873365806E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1525 0.0 54.657475 1 GTATCAA 2520 0.0 52.21353 1 ATCAACG 2760 0.0 46.364674 3 TCAACGC 2795 0.0 45.784077 4 CAACGCA 2885 0.0 44.355804 5 AACGCAG 2945 0.0 43.64975 6 TATCAAC 3415 0.0 38.343327 2 GTACATG 6910 0.0 37.566418 1 ACATGGG 7255 0.0 34.292305 3 ACGCAGA 3775 0.0 34.04212 7 TACATGG 7605 0.0 33.80978 2 CGCAGAG 3850 0.0 33.069897 8 CATGGGA 5185 0.0 29.845743 4 CATGGGG 2780 0.0 26.334078 4 GCAGAGT 4825 0.0 26.017462 9 ATGGGAG 2350 0.0 24.567541 5 ATGGGAT 2315 0.0 24.424767 5 TAAGGTG 855 0.0 24.364624 5 GTAAGGT 850 0.0 23.107492 4 AGAGTAC 4355 0.0 21.994675 10-11 >>END_MODULE