FastQCFastQC Report
Thu 26 May 2016
SRR1364375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464407
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.21380668079297627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29380.2006272846278391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16420.11212729794380934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.073.658141
GTATCAA44700.055.9680441
ATCAACG55900.044.104513
TCAACGC56650.043.5206074
CAACGCA57850.042.51495
AACGCAG59400.041.676366
TATCAAC59800.041.42732
GTACATG71650.036.4129871
ACGCAGA70950.034.7169727
CGCAGAG72250.034.1746568
ACATGGG74150.034.1328283
TACATGG76800.033.652942
GTGGTAT11250.030.7095761
GCAGAGT80500.030.154929
CATGGGA51600.029.8912814
TGGTATC12500.028.1084232
GAGTACT44700.027.28602412-13
CAGAGTA79750.025.85035110-11
GTACTTT48100.025.29543714-15
TAAGGTG8050.025.1522885
Produced by FastQC (version 0.11.5)