##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1464407 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30973356450768 33.0 31.0 34.0 30.0 34.0 2 32.31649261441662 33.0 31.0 34.0 30.0 34.0 3 32.432338140967644 34.0 31.0 34.0 30.0 34.0 4 36.037833061437155 37.0 35.0 37.0 35.0 37.0 5 35.973939621976676 37.0 35.0 37.0 35.0 37.0 6 36.033257830644075 37.0 35.0 37.0 35.0 37.0 7 35.98855714292543 37.0 35.0 37.0 35.0 37.0 8 35.99031348525376 37.0 35.0 37.0 35.0 37.0 9 37.74174870783874 39.0 38.0 39.0 35.0 39.0 10-11 37.70356635825969 39.0 37.5 39.0 35.0 39.0 12-13 37.675266165758565 39.0 37.0 39.0 35.0 39.0 14-15 39.063207837711786 41.0 38.0 41.0 35.5 41.0 16-17 39.06965891313003 41.0 38.0 41.0 36.0 41.0 18-19 39.134831368601766 41.0 38.5 41.0 36.0 41.0 20-21 39.15094061965014 41.0 39.0 41.0 35.5 41.0 22-23 39.11460987280175 41.0 39.0 41.0 35.0 41.0 24-25 39.08504841891633 41.0 39.0 41.0 35.0 41.0 26-27 38.99513864656478 41.0 39.0 41.0 35.0 41.0 28-29 38.91164409894244 41.0 38.0 41.0 35.0 41.0 30-31 38.80337638375124 40.0 38.0 41.0 35.0 41.0 32-33 38.683286818486934 40.0 38.0 41.0 34.5 41.0 34-35 38.597511484170724 40.0 38.0 41.0 34.0 41.0 36-37 38.486526286749516 40.0 38.0 41.0 34.0 41.0 38-39 38.40939574858629 40.0 38.0 41.0 34.0 41.0 40-41 38.30491830481553 40.0 38.0 41.0 34.0 41.0 42-43 38.15996714028272 40.0 38.0 41.0 33.0 41.0 44-45 37.99744845524502 40.0 38.0 41.0 33.0 41.0 46-47 37.77287291033162 40.0 37.5 41.0 33.0 41.0 48-49 37.565285811936164 40.0 37.0 41.0 33.0 41.0 50-51 37.41170487439626 40.0 37.0 41.0 32.0 41.0 52-53 37.263197321509665 40.0 37.0 41.0 32.0 41.0 54-55 37.08355532307617 40.0 36.0 41.0 31.0 41.0 56-57 36.89188866210009 39.5 36.0 41.0 31.0 41.0 58-59 36.685328259152 39.0 35.5 41.0 31.0 41.0 60-61 36.43808312852916 39.0 35.0 41.0 31.0 41.0 62-63 36.203623377927045 38.5 35.0 41.0 31.0 41.0 64-65 35.915008942186155 38.0 35.0 40.0 30.0 41.0 66-67 35.58253545633147 37.0 35.0 40.0 30.0 41.0 68-69 35.252016686617864 37.0 35.0 39.5 29.0 41.0 70-71 34.98452889121672 36.0 34.5 39.0 29.5 41.0 72-73 34.776588407457766 36.0 34.5 39.0 30.0 41.0 74-75 34.4362800095875 35.5 34.0 38.5 29.0 40.0 76-77 34.09737627585774 35.0 34.0 37.0 29.0 39.0 78-79 33.769335983780465 35.0 34.0 37.0 29.0 39.0 80-81 33.467380311621014 35.0 34.0 36.5 29.0 39.0 82-83 33.188472193864136 35.0 34.0 36.0 29.0 37.0 84-85 32.954905296136936 35.0 34.0 36.0 29.0 37.0 86-87 32.76447702039119 35.0 34.0 35.0 29.0 36.5 88-89 32.60292288960651 35.0 34.0 35.0 28.5 36.0 90-91 32.4653928177071 35.0 33.5 35.0 28.0 36.0 92-93 32.34124324726664 35.0 33.0 35.0 27.5 36.0 94-95 32.255269539137686 35.0 33.0 35.0 27.0 35.5 96-97 32.16782697706307 35.0 33.0 35.0 27.0 35.0 98-99 32.1033701696318 35.0 33.0 35.0 27.0 35.0 100-101 32.04570450701205 35.0 33.0 35.0 27.0 35.0 102-103 31.057126877978597 34.0 31.5 35.0 24.5 35.0 104-105 31.78589388059467 35.0 33.0 35.0 25.5 35.0 106-107 32.080899982040506 35.0 33.0 35.0 27.0 35.0 108-109 32.09967242713262 35.0 34.0 35.0 27.0 35.0 110-111 32.06915290626172 35.0 34.0 35.0 27.0 35.0 112-113 31.999475897069598 35.0 33.5 35.0 27.0 35.0 114-115 31.927453228508195 35.0 33.0 35.0 27.0 35.0 116-117 31.865308619803102 35.0 33.0 35.0 27.0 35.0 118-119 31.757695435763416 35.0 33.0 35.0 26.0 35.0 120-121 31.616011805461188 35.0 33.0 35.0 25.0 35.0 122-123 31.473056670720638 35.0 33.0 35.0 25.0 35.0 124-125 30.768964502354876 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20616098349279355 1101 2 0.1510872356519002 1101 3 0.18081354392943183 1101 4 0.20779609292360135 1101 5 0.26421323972191146 1101 6 0.2700540283296746 1101 7 0.3092241014315462 1101 8 0.26521371470621347 1101 9 0.23796902911374218 1101 10-11 0.32918478991636135 1101 12-13 0.2982537758734196 1101 14-15 0.3694704749507096 1101 16-17 0.3862112174328587 1101 18-19 0.32101874761572446 1101 20-21 0.29529469602513814 1101 22-23 0.3008370546561636 1101 24-25 0.3192496650739258 1101 26-27 0.32470051911650444 1101 28-29 0.24593655083443622 1101 30-31 0.3130772633932537 1101 32-33 0.31163376893758254 1101 34-35 0.3116353368053737 1101 36-37 0.386747459365381 1101 38-39 0.40230903286892783 1101 40-41 0.3572347948736905 1101 42-43 0.39265140401648324 1101 44-45 0.38670461877482865 1101 46-47 0.3908824462767626 1101 48-49 0.3892872960368976 1101 50-51 0.3422992707044088 1101 52-53 0.3813483834975102 1101 54-55 0.3902017120849308 1101 56-57 0.3571119608609692 1101 58-59 0.12398353937705764 1101 60-61 0.2478123582484386 1101 62-63 0.1711389731426891 1101 64-65 0.2538182910767688 1101 66-67 0.24661037126520569 1101 68-69 0.2615063399136517 1101 70-71 0.3005640794293072 1101 72-73 0.2906628272091467 1101 74-75 0.33619060806050527 1101 76-77 0.16266496632883332 1101 78-79 -0.06305398255452843 1101 80-81 -0.02118463221376743 1101 82-83 -0.05217230109644788 1101 84-85 -0.10326985301956171 1101 86-87 -0.048059517472317737 1101 88-89 -0.14285197575034658 1101 90-91 -0.41837639709503804 1101 92-93 -0.393495952150829 1101 94-95 -0.23647512645442959 1101 96-97 -0.15636787665197005 1101 98-99 -0.05638881632875581 1101 100-101 -0.12114438386153381 1101 102-103 0.10933318034156869 1101 104-105 0.09859219631049854 1101 106-107 0.1624630234642268 1101 108-109 0.19579167432489442 1101 110-111 0.16321042385246187 1101 112-113 0.10493601173241274 1101 114-115 0.033282777985572665 1101 116-117 0.1513423527707829 1101 118-119 0.16895703085403468 1101 120-121 0.16426976680533656 1101 122-123 0.2373263503017391 1101 124-125 0.2676990406143638 1102 1 0.08048003110251756 1102 2 0.1369346514102503 1102 3 0.09836133432717276 1102 4 0.06069188504518763 1102 5 5.69228284973633E-4 1102 6 0.05360052605727361 1102 7 0.14001179700446897 1102 8 0.20147084946435712 1102 9 0.2515333636007355 1102 10-11 0.20530915948948802 1102 12-13 0.17491200820466446 1102 14-15 0.20988673561845417 1102 16-17 0.2847730512057467 1102 18-19 0.29439707422355355 1102 20-21 0.235669143969929 1102 22-23 0.19864160388912921 1102 24-25 0.22076123199157394 1102 26-27 0.3311566907026986 1102 28-29 0.2773068616691816 1102 30-31 0.21484129474887936 1102 32-33 0.15104060694939392 1102 34-35 0.14066404813595312 1102 36-37 0.19659746655534605 1102 38-39 0.22907252579329196 1102 40-41 0.0964278267246712 1102 42-43 0.1535794376198183 1102 44-45 0.17208646008793238 1102 46-47 0.2236463477562438 1102 48-49 0.22449578423720595 1102 50-51 0.094520272482427 1102 52-53 0.0916987945653176 1102 54-55 0.2726580333441291 1102 56-57 0.20973929876759456 1102 58-59 0.2313612007231498 1102 60-61 0.26517072961459576 1102 62-63 0.16240170214729233 1102 64-65 0.26639974376215037 1102 66-67 0.06647498245148142 1102 68-69 0.17077414518296763 1102 70-71 0.14870536031268955 1102 72-73 0.2525573394959295 1102 74-75 0.11476123947396388 1102 76-77 0.19247219488279654 1102 78-79 0.19129050511670442 1102 80-81 0.14538120096129603 1102 82-83 0.15277998301599638 1102 84-85 0.20087495823254642 1102 86-87 0.12302108906017395 1102 88-89 0.08554360567601549 1102 90-91 0.12389970306143994 1102 92-93 0.21239324587374142 1102 94-95 0.1073944909460991 1102 96-97 0.04163614232952284 1102 98-99 0.11201083759591057 1102 100-101 0.06938816708221651 1102 102-103 0.024000309080157933 1102 104-105 -0.1183032546958458 1102 106-107 -0.13519331032573234 1102 108-109 -0.03151540575255751 1102 110-111 -0.025865752097729455 1102 112-113 -0.07095153510294949 1102 114-115 -0.1393663495273536 1102 116-117 -0.14867676969720378 1102 118-119 -0.10985388231016557 1102 120-121 -0.034267788612293515 1102 122-123 -0.0761493116260823 1102 124-125 -0.3435932879692416 1103 1 0.06811276439414371 1103 2 0.02597606267453756 1103 3 0.10156445572283701 1103 4 0.07176349437619223 1103 5 -0.0327224164408193 1103 6 0.010787242762390292 1103 7 0.06778179588857824 1103 8 0.039302999448800335 1103 9 0.15147775101343086 1103 10-11 0.1303558006551242 1103 12-13 0.10226803762501646 1103 14-15 0.16015244651526928 1103 16-17 0.198111958676364 1103 18-19 0.20272528075966534 1103 20-21 0.07054544062161483 1103 22-23 0.13990475733418606 1103 24-25 0.20444983513837656 1103 26-27 0.07580400758285322 1103 28-29 0.11489265266448712 1103 30-31 0.21234929630249155 1103 32-33 0.14895916894015215 1103 34-35 0.1532144932070949 1103 36-37 0.051208390940175263 1103 38-39 0.09463574357805271 1103 40-41 0.03836664924233446 1103 42-43 0.10501160378576913 1103 44-45 0.034356444540499353 1103 46-47 0.02793628955961225 1103 48-49 -0.007044848064509779 1103 50-51 -0.11726300524210842 1103 52-53 -0.013963757709269942 1103 54-55 -0.01871931112869163 1103 56-57 -0.018696505738823532 1103 58-59 9.306611174224599E-4 1103 60-61 -0.025457424445221477 1103 62-63 -0.05940922732835219 1103 64-65 -0.1439627425818628 1103 66-67 -0.11463773746766748 1103 68-69 -0.08964582871298177 1103 70-71 -0.10525121277922977 1103 72-73 -0.06955681153257842 1103 74-75 -0.002666659045225117 1103 76-77 -0.04540343155424864 1103 78-79 -0.012920642718462716 1103 80-81 0.04398254552683767 1103 82-83 -0.04861860423792308 1103 84-85 8.405497907020276E-4 1103 86-87 -0.03681510039352531 1103 88-89 -0.007818155908935864 1103 90-91 0.004320140585008403 1103 92-93 -0.009771540563235703 1103 94-95 0.03311282398663451 1103 96-97 -0.04288067246692151 1103 98-99 0.08494350727509214 1103 100-101 -0.03065449120399677 1103 102-103 0.11038140689126763 1103 104-105 0.1182094429055418 1103 106-107 0.029720851520757208 1103 108-109 0.027350642910612066 1103 110-111 0.019654866490597556 1103 112-113 0.10007251148889651 1103 114-115 0.09997572651984754 1103 116-117 0.09580361851365637 1103 118-119 0.07638987718255308 1103 120-121 0.20921955014927107 1103 122-123 0.1929716345015251 1103 124-125 -0.03818859446687384 1104 1 0.03242493049419437 1104 2 0.030540218164503585 1104 3 0.06573021953649061 1104 4 0.02003235155107319 1104 5 0.10806918329019766 1104 6 0.08953065816195505 1104 7 0.11272426235848343 1104 8 0.11241448000320986 1104 9 0.1735142138221093 1104 10-11 0.10608115743772117 1104 12-13 0.18675733414685425 1104 14-15 0.10159059797042147 1104 16-17 0.12929471169313445 1104 18-19 0.11766275040511687 1104 20-21 0.11566536440835762 1104 22-23 0.059239899486840386 1104 24-25 0.09164477200093302 1104 26-27 0.005146107930301014 1104 28-29 0.034577455780258504 1104 30-31 0.09047943322482155 1104 32-33 0.1063858743983701 1104 34-35 0.1265920429885412 1104 36-37 0.2262430651659031 1104 38-39 0.12481399478289035 1104 40-41 0.07693057590576302 1104 42-43 0.002071999982582895 1104 44-45 0.026956357679807752 1104 46-47 0.18307916604931762 1104 48-49 0.0626965779451325 1104 50-51 0.032571796005697706 1104 52-53 0.1752301424289584 1104 54-55 0.1246093444920433 1104 56-57 0.12976265105872642 1104 58-59 0.21638018852056717 1104 60-61 0.13949277075781907 1104 62-63 0.11155175074165413 1104 64-65 0.043548581996915914 1104 66-67 -0.031219372503343834 1104 68-69 0.06971294328238997 1104 70-71 0.0035493859057211807 1104 72-73 0.10410268523582289 1104 74-75 0.18250538115353265 1104 76-77 0.16737174403509414 1104 78-79 0.20878413835524867 1104 80-81 0.23278288736567276 1104 82-83 0.23176640809069227 1104 84-85 0.1944114579985694 1104 86-87 0.1349949875879446 1104 88-89 0.17008677366852254 1104 90-91 0.18081405099708547 1104 92-93 0.2141534416910318 1104 94-95 0.2054996482478586 1104 96-97 0.16681398419986948 1104 98-99 0.11089395465432972 1104 100-101 0.18597898004791347 1104 102-103 0.14488261173304906 1104 104-105 0.12041925349320337 1104 106-107 0.19771362247006152 1104 108-109 0.29630261735675845 1104 110-111 0.23148099567951874 1104 112-113 0.1889713888378708 1104 114-115 0.1187383353292013 1104 116-117 0.07987939278794798 1104 118-119 0.0549582869781986 1104 120-121 -0.05906497463871219 1104 122-123 -0.018553517738173042 1104 124-125 -0.1763274408075155 1105 1 0.12947163379089233 1105 2 0.08976640864069907 1105 3 0.1273602561665257 1105 4 0.1383931574118762 1105 5 0.1156470221180328 1105 6 0.08852333215462949 1105 7 0.12682682646104837 1105 8 0.082561000149731 1105 9 0.13038234569024354 1105 10-11 0.12642227465384082 1105 12-13 0.11404671143623091 1105 14-15 0.2061235650033879 1105 16-17 0.18710148824990824 1105 18-19 0.2275757540681269 1105 20-21 0.17784485158784946 1105 22-23 0.1426877016846433 1105 24-25 0.20010402108518122 1105 26-27 0.211235851520037 1105 28-29 0.1374277305055358 1105 30-31 0.26780315117353837 1105 32-33 0.20117754106503583 1105 34-35 0.15345788547939065 1105 36-37 0.14576000655784327 1105 38-39 0.24855483727372985 1105 40-41 0.25890172975191916 1105 42-43 0.21884168862727194 1105 44-45 0.16494857379702665 1105 46-47 0.05953292062807236 1105 48-49 0.16693192593047712 1105 50-51 0.08523892054782323 1105 52-53 0.13297968455350428 1105 54-55 0.08240467416236186 1105 56-57 0.08006027010634398 1105 58-59 0.06390766104804158 1105 60-61 -0.0586413867513329 1105 62-63 -0.06706546537556335 1105 64-65 -0.17901323118989865 1105 66-67 -0.12399638715535843 1105 68-69 -0.13452240470295607 1105 70-71 -0.06507469834336632 1105 72-73 0.08819151307464779 1105 74-75 0.19532589397403655 1105 76-77 0.1141780627164124 1105 78-79 0.17145584165194805 1105 80-81 0.16980211813490342 1105 82-83 0.21414964984892748 1105 84-85 0.1458766594637666 1105 86-87 0.03757053703849067 1105 88-89 0.034944832276586624 1105 90-91 0.08727009495312643 1105 92-93 0.1738146138522012 1105 94-95 0.13165578377898868 1105 96-97 0.10645455196543452 1105 98-99 0.09414715978252985 1105 100-101 0.08617357711751339 1105 102-103 0.06496045056088917 1105 104-105 0.06327639635034998 1105 106-107 0.06644072869717377 1105 108-109 0.12038686644100949 1105 110-111 0.08225938395791843 1105 112-113 -0.023047841931358448 1105 114-115 0.021325331666201208 1105 116-117 -0.012817493658939583 1105 118-119 -0.005607189212277319 1105 120-121 -0.030848399547135585 1105 122-123 -9.939939286489619E-4 1105 124-125 -0.275457477437552 1106 1 0.07780315301793905 1106 2 0.08778828725959187 1106 3 0.10405520301072357 1106 4 0.08676816548837962 1106 5 0.1093523217934873 1106 6 0.007718564782692283 1106 7 0.08315967271926894 1106 8 0.06882908529841103 1106 9 0.12808924078519368 1106 10-11 0.11578210636442066 1106 12-13 0.13151866734101958 1106 14-15 0.13689397618424692 1106 16-17 0.11423995923166785 1106 18-19 0.04950071964980651 1106 20-21 -0.007600300275214522 1106 22-23 -0.1691667995355317 1106 24-25 -0.018309716918111008 1106 26-27 -0.03986659081374455 1106 28-29 -0.1332487323351117 1106 30-31 -0.09776411737880153 1106 32-33 0.026089258700629614 1106 34-35 -0.05597664764918164 1106 36-37 -0.06925072897527684 1106 38-39 -0.16985014083273597 1106 40-41 -0.13230873888392125 1106 42-43 -0.1452174487527671 1106 44-45 -0.18526216074206303 1106 46-47 -0.1521810494814133 1106 48-49 -0.2221177681565294 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32-33 -0.5126652687090782 2216 34-35 -0.5244021708248283 2216 36-37 -0.5217453695257888 2216 38-39 -0.5672022687111422 2216 40-41 -0.8322607356298732 2216 42-43 -0.6304915491611567 2216 44-45 -0.5866770354700179 2216 46-47 -0.7862718838009073 2216 48-49 -0.5741573332061733 2216 50-51 -0.49311262014758483 2216 52-53 -0.6455554436785391 2216 54-55 -0.5227700922908696 2216 56-57 -0.6782585296341992 2216 58-59 -0.5562311549485983 2216 60-61 -0.6389346087142869 2216 62-63 -0.5462444115824212 2216 64-65 -0.5194111690345693 2216 66-67 -0.5809044527610752 2216 68-69 -0.6876808706553277 2216 70-71 -0.5270020680591259 2216 72-73 -0.6619364969266144 2216 74-75 -0.6783576720313462 2216 76-77 -0.5828237782053378 2216 78-79 -0.5285733264613484 2216 80-81 -0.3839726798077834 2216 82-83 -0.440038648994026 2216 84-85 -0.48133248028210573 2216 86-87 -0.4557737839601259 2216 88-89 -0.4414626385283853 2216 90-91 -0.45045467109639503 2216 92-93 -0.46852897508396296 2216 94-95 -0.48009829302835527 2216 96-97 -0.5189256091421299 2216 98-99 -0.5328322011700095 2216 100-101 -0.46775042564924973 2216 102-103 -0.47487555533867365 2216 104-105 -0.4438349713414773 2216 106-107 -0.3130724228585997 2216 108-109 -0.2562725536148136 2216 110-111 -0.23277322915515697 2216 112-113 -0.13360357018546765 2216 114-115 -0.12376752857177564 2216 116-117 -0.1710457612277665 2216 118-119 -0.15396455680183152 2216 120-121 -0.08011761551363605 2216 122-123 -0.1131258768632506 2216 124-125 -0.08246616798775719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 201.0 3 16.0 4 28.0 5 87.0 6 236.0 7 452.0 8 787.0 9 1445.0 10 2275.0 11 3167.0 12 4210.0 13 6235.0 14 9254.0 15 7852.0 16 3978.0 17 3561.0 18 3490.0 19 3389.0 20 3292.0 21 3405.0 22 3680.0 23 4107.0 24 5075.0 25 6831.0 26 9274.0 27 12462.0 28 15795.0 29 20229.0 30 25718.0 31 32589.0 32 41551.0 33 53570.0 34 74002.0 35 112154.0 36 226251.0 37 503292.0 38 259609.0 39 858.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.607138295423574 18.860050257949368 13.400252860734662 24.132558585892394 2 15.641211766947302 20.62630129465374 39.12785175159638 24.604635186802575 3 18.461943981420465 25.462047094830876 30.081391307198068 25.994617616550592 4 12.602507363048659 17.045329611235125 37.516004771897435 32.83615825381878 5 12.918288575815156 38.66583581189065 34.654365096446796 13.761510515847394 6 31.56900082095862 37.41631617698283 17.320997553515983 13.693685448542558 7 28.101067531089374 33.051125814066715 21.26198522678463 17.585821428059276 8 26.520564296674355 35.07160236191168 19.87903636079314 18.528796980620825 9 26.876203132052765 15.459295127652354 19.71268916360001 37.95181257669486 10-11 24.65810392875751 26.723240192105063 27.94366593440212 20.67498994473531 12-13 25.7158699733066 24.059226704051536 28.220842976030568 22.00406034661129 14-15 22.611951458849898 25.382663426219626 25.973448638254254 26.031936476676226 16-17 22.074634988770196 28.91999969953708 26.716274915375305 22.28909039631742 18-19 22.160471781410497 27.237373216598936 28.857414639509372 21.744740362481195 20-21 22.78672908109437 26.43302228550162 29.094820080510512 21.685428552893494 22-23 22.582451463288553 26.351997771111446 28.95964031857264 22.105910447027362 24-25 22.43126398603667 26.694457210324725 28.863901907051797 22.01037689658681 26-27 22.08058661587907 26.952583635817955 28.724231331682372 22.242598416620602 28-29 22.1691479369259 26.813493129801223 28.852752091379124 22.164606841893754 30-31 22.19434589281855 26.556767614412546 28.897855749555536 22.351030743213368 32-33 21.903469327132864 26.789412764911347 28.82150088261103 22.485617025344766 34-35 22.57223153013288 26.682841603640522 28.484801511603173 22.260125354623426 36-37 22.62304810069878 26.289685859190786 28.506931474651516 22.58033456545892 38-39 22.233341630209917 26.59534767156524 28.601450485230707 22.56986021299414 40-41 22.514392359220174 26.363462852331544 28.795576088726794 22.32656869972149 42-43 22.57661292250037 26.26121016903088 28.50372198439368 22.65845492407507 44-45 22.33390956261773 26.542784768289927 28.417368003602828 22.70593766548951 46-47 22.712060240083527 26.739526647987887 27.79763412767079 22.750778984257792 48-49 22.697342700989246 26.84528583539817 27.61727409953766 22.840097364074925 50-51 22.670507584298626 26.855785311050823 27.690252778086965 22.783454326563586 52-53 22.941811941625517 26.72347919669873 27.584339599578534 22.750369262097216 54-55 22.614204930733052 26.78951275157794 27.668913082223728 22.927369235465278 56-57 22.61308591569349 27.02203930397741 27.581788253466506 22.78308652686259 58-59 22.600684099434105 26.549893574668793 27.68605312594108 23.163369199956023 60-61 22.38899431647076 26.683975151716698 28.08194716359591 22.845083368216624 62-63 22.401524985881657 26.807813674750257 28.094853411654004 22.695807927714085 64-65 22.23893357516046 26.6348084924478 27.983955280191914 23.142302652199835 66-67 22.23131957167645 26.905600697073968 27.630262625076224 23.23281710617335 68-69 22.276977055209738 26.62522325597435 27.988198632005524 23.109601056810387 70-71 22.35174374337189 26.555800402483737 27.706983099643743 23.38547275450063 72-73 22.37652835110506 26.667212963626863 27.50106698625039 23.45519169901769 74-75 22.17966043593072 26.93322279939935 27.45585755872514 23.431259205944794 76-77 22.344820239462198 26.607229618278804 27.679609452703197 23.368340689555804 78-79 22.408695123691704 26.61309321793736 27.58430545606515 23.393906202305782 80-81 22.395482116426322 26.92941319070406 27.305197853732153 23.369906839137464 82-83 22.459090949442334 26.774865184337415 27.370191483651745 23.395852382568506 84-85 22.299435880871915 26.620570647367842 27.417548536711443 23.662444935048796 86-87 22.36622059304551 26.663557330714756 27.411470991329594 23.55875108491014 88-89 22.280588661485503 26.682780128748362 27.338984312421346 23.697646897344796 90-91 22.22493473467417 26.770528958138 27.337106419185375 23.66742988800245 92-93 22.32060485916825 26.384638969903857 27.49406415019868 23.800692020729212 94-95 22.00771370254308 26.157004166191506 27.617834386205477 24.217447745059946 96-97 22.252386119432643 26.078986238115498 27.548147475394476 24.12048016705738 98-99 22.06864080043376 26.221519168864372 27.324730983074364 24.385109047627502 100-101 21.821904702722673 26.07325012786746 27.684448380812164 24.420396788597706 102-103 21.983437664529053 25.871700968378324 27.59461679710627 24.550244569986347 104-105 21.786542883823838 26.027379954486246 27.52479270414182 24.661284457548096 106-107 21.92857652571195 25.968677412999735 27.49502955634245 24.60771650494586 108-109 21.99784277967979 25.84297361792617 27.28043761730611 24.87874598508793 110-111 21.793667524732125 26.169903789453343 27.34036243619568 24.696066249618855 112-113 21.722137356622852 26.16127210536415 27.564980227491404 24.551610310521596 114-115 21.692261782414317 26.195108327124906 27.267999948101863 24.84462994235892 116-117 21.452949897125595 26.22177441107561 27.41447562050714 24.910800071291657 118-119 21.35796264289914 26.355958418663665 27.03920426493454 25.24687467350265 120-121 21.265245541956162 26.51874978446856 27.193188216225924 25.022816457349357 122-123 21.285672874326387 26.54936269142219 27.166919159068847 24.998045275182577 124-125 21.1632152684381 26.41186036800574 27.236153349496877 25.18877101405928 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 96.0 1 77.5 2 118.5 3 175.5 4 120.0 5 102.0 6 236.0 7 609.5 8 1056.0 9 1260.0 10 1003.0 11 645.0 12 606.0 13 668.5 14 752.0 15 857.0 16 951.0 17 1067.5 18 1171.5 19 1281.0 20 1451.5 21 1691.0 22 2029.0 23 2387.0 24 2961.5 25 3959.5 26 5196.5 27 6802.5 28 8796.0 29 11367.0 30 14664.5 31 18103.0 32 22541.5 33 26153.0 34 28588.0 35 31716.0 36 36395.5 37 40839.0 38 42911.5 39 44655.5 40 45635.5 41 47511.5 42 49708.0 43 52353.0 44 55856.0 45 58488.0 46 57954.5 47 57260.0 48 57108.0 49 55128.5 50 54393.5 51 52635.5 52 50863.5 53 50566.0 54 49951.0 55 46838.0 56 42233.5 57 38277.0 58 35162.5 59 31209.5 60 25552.5 61 20335.5 62 16258.0 63 12227.0 64 9003.5 65 6972.5 66 5181.0 67 4312.0 68 3203.5 69 1902.0 70 1280.0 71 849.0 72 621.5 73 479.5 74 394.0 75 348.0 76 208.0 77 70.5 78 22.0 79 13.5 80 11.0 81 7.0 82 2.5 83 1.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02458332963445272 2 0.0 3 0.0 4 0.0 5 0.05831712085506283 6 0.018096062092027694 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.365740535247373E-4 22-23 0.0 24-25 0.0 26-27 5.121527007177649E-4 28-29 1.7071756690592165E-4 30-31 1.7071756690592165E-4 32-33 1.365740535247373E-4 34-35 1.0243054014355298E-4 36-37 0.0 38-39 3.4143513381184327E-5 40-41 1.0243054014355298E-4 42-43 0.0 44-45 1.0243054014355298E-4 46-47 0.0 48-49 4.438656739553963E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.4143513381184327E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.4143513381184327E-5 70-71 0.0 72-73 1.365740535247373E-4 74-75 0.0 76-77 3.4143513381184327E-5 78-79 0.0 80-81 3.0729162043065895E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.828702676236865E-5 100-101 0.0 102-103 0.0 104-105 9.901618880543456E-4 106-107 3.4143513381184327E-5 108-109 0.001536458102153295 110-111 0.004541087279697515 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.3900459366829033E-4 122-123 4.438656739553963E-4 124-125 3.4143513381184327E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1464407.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.77832499628057 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14592063801618 52.39129223570066 2 10.76805706677709 13.735372863668097 3 3.1704877267395215 6.066251898981359 4 1.3470916817555376 3.4366100431516333 5 0.6910642839439411 2.2037461247349293 6 0.3957315830262237 1.514345850812345 7 0.2702816544232515 1.206667783743697 8 0.18964879578138116 0.9676386025998537 9 0.12487952058886481 0.7168145984546697 >10 0.7873130249341489 9.44410899130442 >50 0.07592530531239917 3.3257945272826523 >100 0.03207150421557149 3.5573033483191527 >500 8.726939919670765E-4 0.3610875097598301 >1k 6.545204939753074E-4 1.0729656214867245 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.21380668079297627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2938 0.2006272846278391 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1642 0.11212729794380934 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.4143513381184327E-5 0.0 22-23 0.0 0.0 0.0 1.365740535247373E-4 0.0 24-25 0.0 0.0 0.0 8.535878345296082E-4 0.0 26-27 0.0 0.0 0.0 0.0014681710753909262 0.0 28-29 0.0 0.0 0.0 0.003721642958549092 0.0 30-31 0.0 0.0 0.0 0.012291664817226359 0.0 32-33 0.0 0.0 0.0 0.03223147663183801 0.0 34-35 0.0 0.0 0.0 0.06395080056295825 0.0 36-37 0.0 0.0 0.0 0.11680495927703158 0.0 38-39 0.0 0.0 0.0 0.198203095177775 0.0 40-41 0.0 0.0 0.0 0.28622507267446823 0.0 42-43 0.0 0.0 0.0 0.3752372120592158 0.0 44-45 0.0 0.0 0.0 0.4808089554338377 0.0 46-47 0.0 0.0 0.0 0.6094958573675215 0.0 48-49 0.0 0.0 0.0 0.750747572225481 3.4143513381184327E-5 50-51 0.0 0.0 0.0 0.9057591229760579 6.828702676236865E-5 52-53 0.0 0.0 0.0 1.0552052810455015 6.828702676236865E-5 54-55 0.0 0.0 0.0 1.2111387066573704 6.828702676236865E-5 56-57 0.0 0.0 0.0 1.403093538886389 6.828702676236865E-5 58-59 0.0 0.0 0.0 1.608705776467881 6.828702676236865E-5 60-61 0.0 0.0 0.0 1.83268722424845 6.828702676236865E-5 62-63 0.0 0.0 0.0 2.0571808247297367 6.828702676236865E-5 64-65 0.0 0.0 0.0 2.2792843792743414 6.828702676236865E-5 66-67 0.0 0.0 0.0 2.5372727663825696 6.828702676236865E-5 68-69 0.0 0.0 0.0 2.8292339493050767 6.828702676236865E-5 70-71 0.0 0.0 0.0 3.1401106386407602 6.828702676236865E-5 72-73 0.0 0.0 0.0 3.4622546873922344 1.0243054014355298E-4 74-75 0.0 0.0 0.0 3.7795503572435805 1.365740535247373E-4 76-77 0.0 0.0 0.0 4.119107597819459 1.365740535247373E-4 78-79 0.0 0.0 0.0 4.508207076311436 1.365740535247373E-4 80-81 0.0 0.0 0.0 4.930835484943735 1.7071756690592165E-4 82-83 0.0 0.0 0.0 5.36975034945886 2.0486108028710599E-4 84-85 0.0 0.0 0.0 5.815801208270651 2.0486108028710599E-4 86-87 0.0 0.0 0.0 6.267110168143146 2.0486108028710599E-4 88-89 0.0 0.0 0.0 6.7476459754699345 2.0486108028710599E-4 90-91 0.0 0.0 0.0 7.281274946104464 2.0486108028710599E-4 92-93 0.0 0.0 0.0 7.836004608008566 3.414351338118433E-4 94-95 0.0 0.0 0.0 8.379364479956735 3.414351338118433E-4 96-97 0.0 0.0 0.0 8.928221457559271 4.0972216057421197E-4 98-99 0.0 0.0 0.0 9.495106210227075 4.780091873365806E-4 100-101 0.0 0.0 0.0 10.126180768051505 4.780091873365806E-4 102-103 0.0 0.0 0.0 10.797851963286163 4.780091873365806E-4 104-105 0.0 0.0 0.0 11.466177094209465 4.780091873365806E-4 106-107 0.0 0.0 0.0 12.115894010340021 4.780091873365806E-4 108-109 0.0 0.0 0.0 12.7973302504017 4.780091873365806E-4 110-111 0.0 0.0 0.0 13.517655952204544 4.780091873365806E-4 112-113 0.0 0.0 0.0 14.267754797675783 4.780091873365806E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2515 0.0 73.65814 1 GTATCAA 4470 0.0 55.968044 1 ATCAACG 5590 0.0 44.10451 3 TCAACGC 5665 0.0 43.520607 4 CAACGCA 5785 0.0 42.5149 5 AACGCAG 5940 0.0 41.67636 6 TATCAAC 5980 0.0 41.4273 2 GTACATG 7165 0.0 36.412987 1 ACGCAGA 7095 0.0 34.716972 7 CGCAGAG 7225 0.0 34.174656 8 ACATGGG 7415 0.0 34.132828 3 TACATGG 7680 0.0 33.65294 2 GTGGTAT 1125 0.0 30.709576 1 GCAGAGT 8050 0.0 30.15492 9 CATGGGA 5160 0.0 29.891281 4 TGGTATC 1250 0.0 28.108423 2 GAGTACT 4470 0.0 27.286024 12-13 CAGAGTA 7975 0.0 25.850351 10-11 GTACTTT 4810 0.0 25.295437 14-15 TAAGGTG 805 0.0 25.152288 5 >>END_MODULE