FastQCFastQC Report
Thu 26 May 2016
SRR1364374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173020
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.055.6616481
GTATCAA21300.051.1538431
ATCAACG23000.044.258933
CAACGCA23450.043.409615
TCAACGC23550.043.2252854
AACGCAG24700.041.4431726
TATCAAC26650.039.5374572
GTACATG60500.036.412721
TACATGG61750.034.89832
TACGGTA700.00278222734.0168882
ACATGGG61150.033.585773
ACGCAGA30500.033.3570947
CGCAGAG30850.032.9772458
GTATAGG5050.031.8331151
CATGGGG24100.028.653234
CATGGGA40300.028.5091924
AATATCG1107.728874E-427.058895
GCAGAGT36950.026.5670249
ATGGGAT18150.024.5989915
GAGTACA44450.024.110571
Produced by FastQC (version 0.11.5)