##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364374_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173020 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.024001295800584 31.0 31.0 34.0 28.0 34.0 2 30.993574704608616 33.0 31.0 34.0 27.0 34.0 3 31.159473836763226 33.0 31.0 34.0 27.0 34.0 4 34.52767727745478 37.0 35.0 37.0 31.0 37.0 5 34.64228231402704 37.0 35.0 37.0 32.0 37.0 6 34.6894835552676 37.0 35.0 37.0 32.0 37.0 7 34.62772672247703 37.0 35.0 37.0 32.0 37.0 8 34.57923564815604 37.0 35.0 37.0 31.0 37.0 9 36.099691394861125 39.0 37.0 39.0 31.0 39.0 10-11 36.01527339687303 39.0 37.0 39.0 31.0 39.0 12-13 36.0181331094099 39.0 37.0 39.0 31.0 39.0 14-15 37.32724164975875 40.0 38.0 41.0 31.0 41.0 16-17 37.33843242229459 40.0 38.0 41.0 31.0 41.0 18-19 37.34441015498457 40.0 38.0 41.0 31.0 41.0 20-21 37.38764002318801 40.0 38.0 41.0 31.0 41.0 22-23 37.35278810250465 40.0 38.0 41.0 31.0 41.0 24-25 37.37317564917904 40.0 38.0 41.0 31.0 41.0 26-27 37.33659741521883 40.0 38.0 41.0 31.0 41.0 28-29 37.3404417656988 40.0 38.0 41.0 31.0 41.0 30-31 37.248072070382435 40.0 38.0 41.0 30.0 41.0 32-33 37.214363352713505 40.0 37.5 41.0 30.0 41.0 34-35 37.20801265110569 40.0 37.0 41.0 30.0 41.0 36-37 37.14287011304155 40.0 37.0 41.0 30.0 41.0 38-39 37.045066580279965 40.0 37.0 41.0 30.0 41.0 40-41 36.99112973350838 40.0 37.0 41.0 30.0 41.0 42-43 36.86037109341699 40.0 37.0 41.0 30.0 41.0 44-45 36.72726424101891 40.0 37.0 41.0 30.0 41.0 46-47 36.617130995208946 40.0 36.0 41.0 30.0 41.0 48-49 36.47984518593033 40.0 36.0 41.0 30.0 41.0 50-51 36.36084721488125 40.0 36.0 41.0 29.0 41.0 52-53 36.21557432950845 40.0 35.5 41.0 29.0 41.0 54-55 36.03889575625309 39.0 35.0 41.0 29.0 41.0 56-57 35.85086486163919 39.0 35.0 41.0 28.0 41.0 58-59 35.6296388808375 39.0 35.0 41.0 28.0 41.0 60-61 35.38638556887351 38.5 35.0 40.5 28.0 41.0 62-63 35.126283013077355 38.0 34.0 40.0 28.0 41.0 64-65 34.785129835808426 37.5 34.0 40.0 27.0 41.0 66-67 34.478410427784695 37.0 34.0 40.0 26.5 41.0 68-69 34.15209033094065 36.5 34.0 39.0 26.0 41.0 70-71 33.79471236637056 36.0 33.5 39.0 26.0 41.0 72-73 33.43246236210806 35.5 33.0 39.0 26.0 40.0 74-75 33.07547228521253 35.0 33.0 37.5 26.0 40.0 76-77 32.84296474058414 35.0 33.0 37.0 26.0 39.0 78-79 32.672517518882884 35.0 33.0 37.0 26.0 39.0 80-81 32.39531337914102 35.0 33.0 36.0 26.0 39.0 82-83 32.096147550766396 35.0 33.0 36.0 25.0 37.0 84-85 31.83964553034049 35.0 33.0 35.5 25.0 37.0 86-87 31.620812518115635 35.0 33.0 35.0 24.5 36.5 88-89 31.354024654311097 35.0 32.0 35.0 24.0 36.0 90-91 31.219429336243202 35.0 32.0 35.0 23.5 36.0 92-93 31.064878262945218 35.0 32.0 35.0 23.0 36.0 94-95 30.95700968440436 35.0 32.0 35.0 22.0 35.5 96-97 30.84671744727285 35.0 32.0 35.0 20.0 35.0 98-99 30.70889371025217 35.0 32.0 35.0 20.0 35.0 100-101 30.609551840548328 35.0 32.0 35.0 19.0 35.0 102-103 29.57732817854768 33.5 29.5 34.5 17.5 35.0 104-105 30.275845680380556 34.5 31.0 35.0 18.0 35.0 106-107 30.60379575795809 35.0 32.0 35.0 19.0 35.0 108-109 30.63047433121345 35.0 32.0 35.0 18.0 35.0 110-111 30.5555340062403 35.0 32.0 35.0 17.0 35.0 112-113 30.45206219842799 35.0 32.0 35.0 12.5 35.0 114-115 30.335906889908102 35.0 32.0 35.0 7.0 35.0 116-117 30.197219996248997 35.0 32.0 35.0 2.0 35.0 118-119 30.019536325041347 35.0 32.0 35.0 2.0 35.0 120-121 29.832612828425773 35.0 32.0 35.0 2.0 35.0 122-123 29.641043204719445 35.0 32.0 35.0 2.0 35.0 124-125 28.822014543656543 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07909103277901863 1101 2 4.92206399098194E-4 1101 3 0.07821687044578951 1101 4 0.008168770521130853 1101 5 -0.1294462447303033 1101 6 -0.09405484274219589 1101 7 0.03160902952525646 1101 8 -0.05050764405827124 1101 9 0.037747399236721435 1101 10-11 0.15225082698033532 1101 12-13 0.15055993655341382 1101 14-15 0.23027233621736087 1101 16-17 0.0659621306319167 1101 18-19 -0.1803236621525457 1101 20-21 0.028166569459600055 1101 22-23 0.14981382251625774 1101 24-25 0.003402894104667098 1101 26-27 0.25262120241185215 1101 28-29 0.08054276110589598 1101 30-31 0.09698897916961613 1101 32-33 0.03148416715426805 1101 34-35 0.07756541364429381 1101 36-37 0.10096489354579319 1101 38-39 0.18540771109024945 1101 40-41 0.1325894464278221 1101 42-43 0.11477233270811382 1101 44-45 0.07811521704140745 1101 46-47 0.03137143190094349 1101 48-49 -0.10163465722210674 1101 50-51 -0.13218698549935226 1101 52-53 -0.020029167560970507 1101 54-55 0.09831067821027517 1101 56-57 0.044799565769146454 1101 58-59 -0.022775778022911197 1101 60-61 -0.0553504236075284 1101 62-63 -0.1123543587359137 1101 64-65 0.11424065170913167 1101 66-67 -0.0674053045777967 1101 68-69 -0.16611991168758067 1101 70-71 -0.13763590297278938 1101 72-73 -0.08218240569343038 1101 74-75 -0.14022007166492756 1101 76-77 -0.12095064098951269 1101 78-79 -0.174180174087617 1101 80-81 -0.1890135506972186 1101 82-83 -0.23292287391888422 1101 84-85 -0.20317663713036183 1101 86-87 -0.14999545433992267 1101 88-89 -0.03649164238794711 1101 90-91 -0.13467657512099152 1101 92-93 -0.025584836693248292 1101 94-95 -0.17370823063597385 1101 96-97 -0.16463629182863215 1101 98-99 -0.10592099635504582 1101 100-101 -0.22246516533362026 1101 102-103 -0.2932546459145726 1101 104-105 -0.13478248869594012 1101 106-107 -0.168987056626694 1101 108-109 -0.20652830673623868 1101 110-111 -0.22283913717268433 1101 112-113 -0.28834855050815733 1101 114-115 -0.361797604988908 1101 116-117 -0.39961784906273223 1101 118-119 -0.2481042758173153 1101 120-121 -0.4213192395058414 1101 122-123 -0.3898134787413028 1101 124-125 -0.4541659330921064 1102 1 0.26577040393568296 1102 2 0.18412474107498156 1102 3 0.26635296849977763 1102 4 0.33973571706250993 1102 5 0.3383441861283032 1102 6 0.37716486059157717 1102 7 0.27546005154749054 1102 8 0.29193740155209724 1102 9 0.3390531036780402 1102 10-11 0.3290486737717089 1102 12-13 0.4090188930019778 1102 14-15 0.34396209893354524 1102 16-17 0.44211214964527557 1102 18-19 0.37017216719858226 1102 20-21 0.2917925687453291 1102 22-23 0.31287524072484274 1102 24-25 0.3372675291132552 1102 26-27 0.30161096411590904 1102 28-29 0.24499023869110204 1102 30-31 0.3206128240900128 1102 32-33 0.2782081582534133 1102 34-35 0.08825627089373 1102 36-37 0.048115709240555304 1102 38-39 0.020476585716309614 1102 40-41 0.058676461099189225 1102 42-43 -0.017445875582865256 1102 44-45 0.13251782165463766 1102 46-47 0.05075330978457515 1102 48-49 0.007268691297809937 1102 50-51 -0.06849928316612619 1102 52-53 0.07918767003314287 1102 54-55 0.025011470120269053 1102 56-57 0.04802930642114944 1102 58-59 0.011062035860170738 1102 60-61 -0.1271925433295138 1102 62-63 -0.10815244265205592 1102 64-65 -0.2375646775256044 1102 66-67 -0.2002425314324654 1102 68-69 -0.23686297976408355 1102 70-71 -0.17837979520246705 1102 72-73 -0.18049936199452077 1102 74-75 -0.2893397459364593 1102 76-77 -0.03340695725612619 1102 78-79 0.05520406781435838 1102 80-81 -0.0955090344823617 1102 82-83 -0.1074587455476319 1102 84-85 -0.15723786882006507 1102 86-87 -0.03273291528301314 1102 88-89 0.06984472199764369 1102 90-91 -0.03883442792723457 1102 92-93 -0.045459941339014875 1102 94-95 -0.11744459415404052 1102 96-97 -0.17393313734617877 1102 98-99 -0.2430284572547592 1102 100-101 -0.23962761315532788 1102 102-103 -0.49276550377128103 1102 104-105 -0.2989908534169743 1102 106-107 -0.30172216801027574 1102 108-109 -0.24060179951188232 1102 110-111 -0.2663376168269451 1102 112-113 -0.20413943392807354 1102 114-115 -0.26004468900481825 1102 116-117 -0.3691953910330916 1102 118-119 -0.42002461779750533 1102 120-121 -0.5078159233736947 1102 122-123 -0.4421730509913395 1102 124-125 -0.5251083522564137 1103 1 0.47788886043998247 1103 2 0.41691962464105714 1103 3 0.4231585267035385 1103 4 0.42885530145858297 1103 5 0.4337137430237732 1103 6 0.35787263101793343 1103 7 0.27077101380491797 1103 8 0.38006183045605013 1103 9 0.610310710347413 1103 10-11 0.5864144377827145 1103 12-13 0.5151630064775716 1103 14-15 0.5023333586669239 1103 16-17 0.3355989779519888 1103 18-19 0.4424681603226972 1103 20-21 0.5661881786209122 1103 22-23 0.6003558252925245 1103 24-25 0.5984278276159003 1103 26-27 0.6552501384815272 1103 28-29 0.6111297247303185 1103 30-31 0.7500169188211956 1103 32-33 0.755903328265866 1103 34-35 0.8197344251406378 1103 36-37 0.6212673380834914 1103 38-39 0.6453645665553509 1103 40-41 0.6894622232621614 1103 42-43 0.5816980486885086 1103 44-45 0.5408360968749051 1103 46-47 0.5801036133179949 1103 48-49 0.6432198018092095 1103 50-51 0.6050506645460416 1103 52-53 0.6008101181596501 1103 54-55 0.5008463153761582 1103 56-57 0.4913754782625759 1103 58-59 0.4809882828846028 1103 60-61 0.3723606633885552 1103 62-63 0.4077632617376281 1103 64-65 0.5491614735766959 1103 66-67 0.5098786472657793 1103 68-69 0.37127272805767575 1103 70-71 0.4079629606962314 1103 72-73 0.3659354219533384 1103 74-75 0.30951505523044887 1103 76-77 0.15635551812165716 1103 78-79 0.2881914556668619 1103 80-81 0.35414357778246597 1103 82-83 0.4194436368120904 1103 84-85 0.367306427699603 1103 86-87 0.30300336044472687 1103 88-89 0.2928391206729195 1103 90-91 0.26992228038880484 1103 92-93 0.1593213517911103 1103 94-95 0.1884135496206305 1103 96-97 0.30485674037509725 1103 98-99 0.15404096749109897 1103 100-101 0.18931575465033035 1103 102-103 0.05911385140903036 1103 104-105 0.17853509616083585 1103 106-107 0.15794525058424824 1103 108-109 0.16900900448868583 1103 110-111 0.19006456239491598 1103 112-113 0.09141789714849935 1103 114-115 0.13451832993173696 1103 116-117 0.15551665922189173 1103 118-119 0.13013338238729588 1103 120-121 -0.007889625014783519 1103 122-123 -0.05243382279786246 1103 124-125 -0.19647365132374262 1104 1 0.19874352332664458 1104 2 0.3642330191050185 1104 3 0.3726671672494817 1104 4 0.3724343990501495 1104 5 0.28583789545276517 1104 6 0.2748296477778496 1104 7 0.21546732235662347 1104 8 0.1951137471864044 1104 9 0.366340379049241 1104 10-11 0.4439413580546443 1104 12-13 0.37925187966504126 1104 14-15 0.3611611266410293 1104 16-17 0.4525600172310078 1104 18-19 0.4656273768758865 1104 20-21 0.4830458566479905 1104 22-23 0.46348524022586446 1104 24-25 0.4186177254963823 1104 26-27 0.38400170595228644 1104 28-29 0.4618905929648349 1104 30-31 0.47390001392228953 1104 32-33 0.38856478431284813 1104 34-35 0.43663495174084943 1104 36-37 0.4036796602837711 1104 38-39 0.3292863827751944 1104 40-41 0.29946587850778883 1104 42-43 0.323888852510251 1104 44-45 0.20677078165806506 1104 46-47 0.2784638246576563 1104 48-49 0.1516175974687357 1104 50-51 0.15062633075390863 1104 52-53 0.1397731127327475 1104 54-55 0.11695388103503745 1104 56-57 0.15707825607436376 1104 58-59 0.11933810563142089 1104 60-61 0.021912327399775222 1104 62-63 0.22640011745686195 1104 64-65 0.3544663018238765 1104 66-67 0.18517058596258806 1104 68-69 0.13893342332386993 1104 70-71 0.13844963304105562 1104 72-73 0.10002609652978833 1104 74-75 -0.09077893844894902 1104 76-77 -0.09865872684276411 1104 78-79 0.15044168783616385 1104 80-81 0.05416841246567117 1104 82-83 0.01897510078168807 1104 84-85 0.00877947419817815 1104 86-87 0.004436601493310377 1104 88-89 0.09791396576221345 1104 90-91 0.022194391564237748 1104 92-93 0.053867865228948375 1104 94-95 -0.02627791441892313 1104 96-97 5.853497552621434E-4 1104 98-99 -0.10058956412852638 1104 100-101 -0.04342073012268699 1104 102-103 -0.18940524943552717 1104 104-105 -0.1883104268628415 1104 106-107 -0.14945128760082937 1104 108-109 -0.13452871362366636 1104 110-111 -0.19124174194188726 1104 112-113 -0.2044247419038676 1104 114-115 -0.25267729216833246 1104 116-117 -0.13746579385017554 1104 118-119 -0.1498664776989962 1104 120-121 -0.21180738373709573 1104 122-123 -0.4740186603493015 1104 124-125 -0.4442959819013552 1105 1 -0.14159771633507034 1105 2 -0.09153954350326643 1105 3 -0.17472740878852377 1105 4 -0.12307863369484551 1105 5 -0.08067940921678485 1105 6 -0.20629898890284437 1105 7 -0.1190941223189057 1105 8 -0.0832482638646681 1105 9 -0.10544338249443541 1105 10-11 -0.0838489737025725 1105 12-13 -0.04872520816174841 1105 14-15 0.12122088215451043 1105 16-17 0.01956124834245543 1105 18-19 0.010142662908393163 1105 20-21 0.09778737146150718 1105 22-23 -0.016854745823685846 1105 24-25 0.0277848448563347 1105 26-27 0.10474496939406208 1105 28-29 -0.00972923467955411 1105 30-31 -0.0783831674239579 1105 32-33 0.0216387699316698 1105 34-35 -0.0827863937385871 1105 36-37 -0.1344341677013503 1105 38-39 -0.14533038155535394 1105 40-41 -0.08320325466760892 1105 42-43 -0.18694155304935123 1105 44-45 -0.2403856080674629 1105 46-47 -0.18622079544964265 1105 48-49 -0.17085790084210117 1105 50-51 -0.1578885888381265 1105 52-53 -0.14974958246913417 1105 54-55 -0.18149935440939657 1105 56-57 -0.23825059211320365 1105 58-59 -0.3859071362675124 1105 60-61 -0.23480671239758522 1105 62-63 -0.14587689226024025 1105 64-65 -0.20511804538169542 1105 66-67 -0.26032332116081847 1105 68-69 -0.1907667383599545 1105 70-71 -0.25650618516588963 1105 72-73 -0.30031286091489307 1105 74-75 -0.324002232491722 1105 76-77 -0.2954644265994304 1105 78-79 -0.04461249071034956 1105 80-81 -0.10516296223256916 1105 82-83 -0.03813882684953285 1105 84-85 -0.1036594122084864 1105 86-87 -0.11651350795667526 1105 88-89 -0.0910857481570595 1105 90-91 -0.11301298481835431 1105 92-93 -0.14240424575751476 1105 94-95 -0.12147127606032271 1105 96-97 -0.12983338885374351 1105 98-99 -0.3375651765502141 1105 100-101 -0.4465549212696125 1105 102-103 -0.46909737514389604 1105 104-105 -0.3641317839140896 1105 106-107 -0.42445442163125335 1105 108-109 -0.34102724625454783 1105 110-111 -0.3602240017224716 1105 112-113 -0.3575600580873548 1105 114-115 -0.4182477982097801 1105 116-117 -0.5137839349040441 1105 118-119 -0.3774048012659392 1105 120-121 -0.4123731164091211 1105 122-123 -0.5118771050344684 1105 124-125 -0.5895362736394105 1106 1 0.1649396000964103 1106 2 0.18453166958813227 1106 3 0.2785676572865903 1106 4 0.3495635019090457 1106 5 0.1443288027954992 1106 6 0.1168734300755645 1106 7 0.013700125020129406 1106 8 0.1869990455842938 1106 9 0.2109498893869528 1106 10-11 0.16518700076592552 1106 12-13 0.0563558858446882 1106 14-15 0.3022510721616811 1106 16-17 0.37883493668533674 1106 18-19 0.24107076512314052 1106 20-21 0.13498979001467148 1106 22-23 0.18404512432483244 1106 24-25 0.13059584901441212 1106 26-27 0.26382674624549907 1106 28-29 0.21115879661298464 1106 30-31 0.3364252939376726 1106 32-33 0.2726929273052576 1106 34-35 0.30485294084498094 1106 36-37 0.2491452281287394 1106 38-39 0.3324332456088115 1106 40-41 0.3388481090810842 1106 42-43 0.3012175880450343 1106 44-45 0.2697991360166512 1106 46-47 0.18408287336314544 1106 48-49 0.15717162295979392 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114-115 -0.7638408691215552 2216 116-117 -0.7932692430145956 2216 118-119 -0.8111812237335876 2216 120-121 -0.6579182032880233 2216 122-123 -0.6831303581784276 2216 124-125 -0.5159298896457187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20754.0 3 3779.0 4 2375.0 5 2048.0 6 2117.0 7 2418.0 8 2710.0 9 2868.0 10 3342.0 11 3744.0 12 4001.0 13 4096.0 14 4094.0 15 3922.0 16 3633.0 17 3469.0 18 3256.0 19 3102.0 20 3061.0 21 3235.0 22 3999.0 23 5305.0 24 7760.0 25 10659.0 26 13098.0 27 13352.0 28 15180.0 29 18918.0 30 22077.0 31 26796.0 32 34601.0 33 45813.0 34 64033.0 35 96762.0 36 187141.0 37 362965.0 38 162118.0 39 419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.477610921967425 18.8285050522969 12.684216092299087 24.009667933436585 2 15.470082409571992 20.268952240643454 39.707499731439945 24.553465618344614 3 18.787026262057914 25.23484955050576 29.361927049365505 26.616197138070817 4 12.241390334667571 16.40438038969952 37.51124297181051 33.8429863038224 5 13.007398008779381 38.99431079822776 34.69048764099363 13.307803551999237 6 32.048725995156396 36.76996282020671 17.6608281884231 13.5204829962138 7 28.502690163575245 32.809850491527115 21.11635607518306 17.57110326971458 8 26.291179070721054 35.87893440589522 19.94549098606414 17.884395537319577 9 26.541150193517588 16.41395713628071 19.554739049632573 37.490153620569124 10-11 24.557732514647054 27.2605054911798 27.854102773402783 20.327659220770364 12-13 25.03416438975111 24.718777839821655 27.870469435339153 22.376588335088083 14-15 22.217713421274844 26.341149235580634 25.07040620554989 26.370731137594632 16-17 22.367824861947923 29.68711037985367 25.855965490710926 22.089099267487484 18-19 22.48454628612154 28.559085573208957 27.136644845479914 21.81972329518959 20-21 23.0186710451105 27.90145432364935 27.078364852417202 22.001509778822946 22-23 22.95690736599008 27.86978392089816 26.794524728702296 22.378783984409466 24-25 22.65067944280575 27.988567969855588 26.9764368893966 22.384315697942064 26-27 22.376643194489436 28.259364716714124 26.847283081277386 22.516709007519054 28-29 22.478517973547664 28.09182828359898 27.00403362103911 22.425620121814248 30-31 22.477565358091113 27.782724654385245 27.06082640640365 22.67888358111999 32-33 22.240333157859965 28.02225883245561 26.87385311813336 22.863554891551065 34-35 22.802314553340224 27.996803103121238 26.657729563836703 22.543152779701835 36-37 22.909626434331894 27.650849942882473 26.648650491892724 22.79087313089291 38-39 22.557274245887854 27.692956249198115 26.91376990217972 22.835999602734315 40-41 22.64833269182324 27.52900968317926 27.105954023547042 22.71670360145045 42-43 22.502067331054295 27.511317039070427 27.074492118566763 22.912123511308515 44-45 22.439887298008106 27.728782059752515 27.144598701625313 22.686731940614063 46-47 22.67050974003416 27.978690891327894 26.678456751997732 22.67234261664022 48-49 22.532011389405127 27.819602393821075 26.675760004092 22.972626212681796 50-51 22.528644012889806 27.674251078412986 26.988073519633083 22.809031389064124 52-53 22.65183926629688 27.635392241403196 26.91702575045279 22.79574274184714 54-55 22.415551067488387 27.67622318064906 26.97514976721822 22.93307598464433 56-57 22.42415614075993 27.931571411704077 26.900385629041658 22.74388681849434 58-59 22.49226349306485 27.551171771766654 26.77419629841178 23.18236843675672 60-61 22.302069342385863 27.589683083327397 27.187724183731447 22.920523390555285 62-63 22.358272855071267 27.622417863089094 27.202813954856346 22.816495326983294 64-65 22.247845053576345 27.60339096623271 27.012043488528125 23.136720491662825 66-67 22.213919585673334 27.769179125949766 26.828717271070662 23.188184017306238 68-69 22.22475613353362 27.446763707418214 27.163126674157574 23.165353484890595 70-71 22.210367478122244 27.354975937663845 27.090865689964577 23.343790894249334 72-73 22.267131407107254 27.362126268505683 26.952956326362372 23.417785998024694 74-75 22.133133843566775 27.585555747372638 27.025194108231727 23.256116300828864 76-77 22.398294970161977 27.372676896845693 27.042583120204604 23.186445012787722 78-79 22.313806864343995 27.264236919527807 27.189769043017453 23.232187173110745 80-81 22.20307382255103 27.56286860550962 27.088231733620233 23.145825838319116 82-83 22.179549587259476 27.465680261003072 27.125104325357007 23.22966582638044 84-85 22.160027211974633 27.308478252159183 27.224165461081846 23.307329074784338 86-87 22.200686702230822 27.311183527671627 27.199120202728476 23.28900956736907 88-89 22.302337675624063 27.295773086891284 27.10889544400491 23.292993793479745 90-91 22.335529526702356 27.407035462903483 27.087644696634182 23.169790313759975 92-93 22.297981896961446 27.07280217730918 27.249527390063577 23.3796885356658 94-95 22.08029360426084 27.00157501124247 27.361845512496725 23.556285871999965 96-97 22.306653236892437 26.94175822911121 27.211447760067962 23.540140773928393 98-99 22.2621811673307 26.809245790421986 27.13588423623168 23.79268880601564 100-101 22.071000670927525 26.812548646508148 27.367832818841897 23.74861786372243 102-103 22.03472625389705 26.645669277913186 27.413356999086957 23.906247469102805 104-105 21.988653970833145 26.747524210161984 27.39351215825618 23.8703096607487 106-107 21.9869030628861 26.861211002041014 27.29989274868579 23.851993186387098 108-109 22.00123018584502 26.796184292581334 27.098914017852614 24.103671503721035 110-111 21.912537233525605 27.056347730012398 27.218282662774655 23.812832373687343 112-113 21.856225910981568 27.074884504852122 27.43366754959322 23.635222034573097 114-115 21.763425588072813 27.21537548679266 27.123515751280497 23.897683173854034 116-117 21.571088051451156 27.195787538917436 27.295702628141953 23.93742178148945 118-119 21.529217007732182 27.270486532219135 27.02967670844058 24.1706197516081 120-121 21.591124665723395 27.451031381608427 27.07208864420862 23.885755308459558 122-123 21.68354689378245 27.433651329531017 27.051842758126803 23.830959018559724 124-125 21.593032162691994 27.481916931055224 27.039074092308585 23.885976813944197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 674.0 1 565.5 2 441.0 3 378.5 4 297.0 5 245.0 6 221.5 7 215.0 8 226.0 9 224.0 10 208.0 11 198.0 12 186.0 13 184.0 14 217.0 15 230.5 16 244.5 17 304.5 18 415.0 19 502.0 20 607.0 21 861.0 22 1198.0 23 1677.0 24 2371.5 25 3280.0 26 4386.0 27 5816.0 28 7405.0 29 9431.5 30 12463.5 31 15676.0 32 19109.0 33 21832.0 34 24158.5 35 26682.0 36 29713.0 37 32928.0 38 34766.5 39 36212.5 40 37111.5 41 38902.0 42 40785.5 43 42514.5 44 44669.5 45 46231.5 46 46046.0 47 45109.5 48 44895.0 49 43792.5 50 42578.5 51 41170.0 52 40175.5 53 40139.5 54 40075.5 55 37771.0 56 33620.5 57 30507.0 58 28805.0 59 25735.5 60 20687.5 61 16396.0 62 12958.0 63 9707.0 64 7053.5 65 5194.5 66 3742.5 67 3000.5 68 2280.0 69 1467.0 70 1015.5 71 683.0 72 517.0 73 371.0 74 268.0 75 208.0 76 135.5 77 73.0 78 42.5 79 31.5 80 22.0 81 19.5 82 20.5 83 14.0 84 7.5 85 6.0 86 9.0 87 8.5 88 5.5 89 4.0 90 2.0 91 2.0 92 2.5 93 2.5 94 2.5 95 2.0 96 2.5 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041261018567458355 2 0.008354503759526693 3 0.0029837513426881042 4 0.005541252493563622 5 0.023188010434604694 6 0.02898501304325587 7 0.0036657516495882426 8 2.557501150875518E-4 9 0.0 10-11 2.1312509590629315E-4 12-13 0.001278750575437759 14-15 4.6887521099384495E-4 16-17 2.1312509590629315E-4 18-19 8.525003836251726E-5 20-21 4.262501918125863E-5 22-23 4.262501918125863E-5 24-25 0.0 26-27 0.0 28-29 3.8362517263132765E-4 30-31 1.278750575437759E-4 32-33 2.1312509590629315E-4 34-35 6.393752877188795E-4 36-37 0.0 38-39 1.278750575437759E-4 40-41 5.541252493563622E-4 42-43 8.525003836251726E-4 44-45 0.001278750575437759 46-47 4.262501918125863E-5 48-49 0.0 50-51 0.0 52-53 9.803754411689485E-4 54-55 0.00281325126596307 56-57 1.278750575437759E-4 58-59 8.525003836251726E-4 60-61 0.0012361255562565003 62-63 7.672503452626553E-4 64-65 0.0017902508056128626 66-67 0.002770626246781811 68-69 0.0035805016112257252 70-71 0.0021312509590629314 72-73 0.003026376361869363 74-75 0.002344376054969225 76-77 0.0017050007672503452 78-79 0.002642751189238035 80-81 0.0015345006905253106 82-83 5.967502685376208E-4 84-85 5.967502685376208E-4 86-87 0.0019181258631566383 88-89 0.005115002301751036 90-91 4.6887521099384495E-4 92-93 0.0010656254795314657 94-95 0.0010656254795314657 96-97 3.8362517263132765E-4 98-99 8.951254028064313E-4 100-101 0.0014492506521627934 102-103 0.0014492506521627934 104-105 8.951254028064313E-4 106-107 0.006308502838826278 108-109 0.0022591260166067075 110-111 0.0015345006905253106 112-113 0.0023017510357879662 114-115 0.003069001381050621 116-117 0.0020460009207004144 118-119 0.044159519871783945 120-121 0.012915380811921366 122-123 0.004262501918125863 124-125 0.0026001261700567767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1173020.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.49913403837003 #Duplication Level Percentage of deduplicated Percentage of total 1 87.4689221952402 66.03827881010199 2 8.105228655152827 12.238754892940419 3 2.0400139702212265 4.620578645336393 4 0.8471688288894098 2.5584205186180204 5 0.4042663727248439 1.5260880530779324 6 0.251750313133216 1.1404158381267782 7 0.156664292522682 0.8279612894137466 8 0.11062744347239047 0.668182094643537 9 0.08144598707171095 0.5534191345333012 >10 0.4893757165604572 6.83443557084856 >50 0.033203475262464456 1.7074941365852154 >100 0.011332749748567305 1.2859710157741944 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.525003836251726E-5 0.0 22-23 8.525003836251726E-5 0.0 0.0 1.7050007672503453E-4 0.0 24-25 1.7050007672503453E-4 0.0 0.0 6.393752877188795E-4 0.0 26-27 1.7050007672503453E-4 0.0 0.0 0.001406625632981535 0.0 28-29 2.983751342688104E-4 0.0 0.0 0.003964126783857053 0.0 30-31 3.4100015345006905E-4 0.0 0.0 0.0130858808886464 0.0 32-33 3.4100015345006905E-4 0.0 0.0 0.03397214028746313 0.0 34-35 3.4100015345006905E-4 0.0 0.0 0.06935090620790779 0.0 36-37 3.4100015345006905E-4 0.0 0.0 0.12506180627781283 0.0 38-39 3.4100015345006905E-4 0.0 0.0 0.20984296942933622 0.0 40-41 3.4100015345006905E-4 0.0 0.0 0.30370326166646777 0.0 42-43 3.4100015345006905E-4 0.0 0.0 0.3909566759305042 0.0 44-45 3.4100015345006905E-4 0.0 0.0 0.5019948508976829 0.0 46-47 5.115002301751036E-4 0.0 0.0 0.6403556631600484 0.0 48-49 6.820003069001381E-4 0.0 0.0 0.7847266031269714 0.0 50-51 6.820003069001381E-4 0.0 0.0 0.9414587986564594 0.0 52-53 6.820003069001381E-4 0.0 0.0 1.084295237932857 0.0 54-55 7.672503452626554E-4 0.0 0.0 1.2323745545685494 0.0 56-57 9.377504219876899E-4 0.0 0.0 1.4195410137934563 0.0 58-59 9.377504219876899E-4 0.0 0.0 1.6310037339516803 0.0 60-61 9.377504219876899E-4 0.0 0.0 1.8580245861110636 0.0 62-63 9.377504219876899E-4 0.0 0.0 2.0826584371962964 0.0 64-65 9.377504219876899E-4 0.0 0.0 2.300600160270072 0.0 66-67 0.0010230004603502072 0.0 0.0 2.559802901911306 0.0 68-69 0.0011082504987127244 0.0 0.0 2.8512727830727522 0.0 70-71 0.0011935005370752416 0.0 0.0 3.1684881758196792 0.0 72-73 0.0011935005370752416 0.0 0.0 3.497297573783908 0.0 74-75 0.0011935005370752416 0.0 0.0 3.809781589401715 0.0 76-77 0.0011935005370752416 0.0 0.0 4.1499718674873405 0.0 78-79 0.0011935005370752416 0.0 0.0 4.531678914255512 0.0 80-81 0.0015345006905253108 0.0 0.0 4.9634277335424795 0.0 82-83 0.0015345006905253108 0.0 0.0 5.399055429574943 0.0 84-85 0.0015771257097065695 0.0 0.0 5.826754872039692 0.0 86-87 0.001619750728887828 0.0 0.0 6.271205947042676 0.0 88-89 0.001619750728887828 0.0 0.0 6.744769910146459 0.0 90-91 0.0018755008439753798 0.0 0.0 7.256781640551738 0.0 92-93 0.0018755008439753798 0.0 0.0 7.803703261666468 0.0 94-95 0.0018755008439753798 0.0 0.0 8.339286627678984 0.0 96-97 0.0018755008439753798 0.0 0.0 8.878493120321904 0.0 98-99 0.0018755008439753798 0.0 0.0 9.446428875892995 0.0 100-101 0.0020460009207004144 0.0 0.0 10.077236534756441 0.0 102-103 0.002301751035787966 4.262501918125863E-5 0.0 10.727992702596715 0.0 104-105 0.0027280012276005524 8.525003836251726E-5 0.0 11.390044500520025 0.0 106-107 0.0027280012276005524 8.525003836251726E-5 0.0 12.02421953589879 0.0 108-109 0.0027280012276005524 8.525003836251726E-5 0.0 12.684651583093213 0.0 110-111 0.0027280012276005524 8.525003836251726E-5 0.0 13.383574022608311 0.0 112-113 0.00281325126596307 8.525003836251726E-5 0.0 14.113655351144907 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1305 0.0 55.661648 1 GTATCAA 2130 0.0 51.153843 1 ATCAACG 2300 0.0 44.25893 3 CAACGCA 2345 0.0 43.40961 5 TCAACGC 2355 0.0 43.225285 4 AACGCAG 2470 0.0 41.443172 6 TATCAAC 2665 0.0 39.537457 2 GTACATG 6050 0.0 36.41272 1 TACATGG 6175 0.0 34.8983 2 TACGGTA 70 0.002782227 34.016888 2 ACATGGG 6115 0.0 33.58577 3 ACGCAGA 3050 0.0 33.357094 7 CGCAGAG 3085 0.0 32.977245 8 GTATAGG 505 0.0 31.833115 1 CATGGGG 2410 0.0 28.65323 4 CATGGGA 4030 0.0 28.509192 4 AATATCG 110 7.728874E-4 27.05889 5 GCAGAGT 3695 0.0 26.567024 9 ATGGGAT 1815 0.0 24.598991 5 GAGTACA 4445 0.0 24.11057 1 >>END_MODULE