FastQCFastQC Report
Thu 26 May 2016
SRR1364374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173020
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.2748461236807556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.2705836217626298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16600.14151506368177866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.075.795481
GTATCAA40850.053.9408261
ATCAACG49200.044.2945023
TCAACGC49600.043.937294
CAACGCA49700.043.729085
TATCAAC51500.042.431922
AACGCAG51600.042.210886
ACGCAGA61150.035.513787
CGCAGAG61800.035.140258
TATCACG851.7031847E-435.0255432
GTACATG58300.033.096681
TACATGG58900.032.2484782
ACATGGG56850.031.3165973
GTGGTAT10150.031.0969491
GCAGAGT70600.030.507349
TGGTATC10600.029.7717152
GAGTACT42150.027.94911612-13
CAGAGTA69550.026.98998810-11
GTACCGT1150.001003798625.8740986
CATGGGG22600.025.5562524
Produced by FastQC (version 0.11.5)