FastQCFastQC Report
Thu 26 May 2016
SRR1364373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602336
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.046.6348651
GTATCAA20550.044.0413361
ATCAACG21200.040.990483
CAACGCA22500.038.6197245
TCAACGC22600.038.4512444
TATCAAC25400.035.385292
AACGCAG24700.035.173326
GTACATG52100.033.1427841
TACATGG55750.030.5351942
ACATGGG54550.029.89663
CGCAGAG30050.029.1010328
ACGCAGA30100.028.8568557
CATGGGG35850.027.3943354
GAGTACA39550.024.0880831
TAAGGTG10200.022.7563865
GTAAGGT8300.022.2305184
GCAGAGT39450.021.2621739
ATGGGGA23050.021.172975
GTGTAGC11350.020.984221
GTATAGG5300.020.2220571
Produced by FastQC (version 0.11.5)