##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364373_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1602336 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05351624128772 31.0 31.0 34.0 28.0 34.0 2 31.060989080941823 33.0 31.0 34.0 27.0 34.0 3 31.132919687256607 33.0 31.0 34.0 27.0 34.0 4 34.557232066183374 37.0 35.0 37.0 32.0 37.0 5 34.59980303756515 37.0 35.0 37.0 32.0 37.0 6 34.665454686158206 37.0 35.0 37.0 32.0 37.0 7 34.55769763645078 37.0 35.0 37.0 31.0 37.0 8 34.504992086553635 37.0 35.0 37.0 31.0 37.0 9 36.03913598645977 39.0 37.0 39.0 31.0 39.0 10-11 35.95304230822998 39.0 37.0 39.0 30.5 39.0 12-13 36.002418344217446 39.0 37.0 39.0 31.0 39.0 14-15 37.322404913825814 40.0 38.0 41.0 31.0 41.0 16-17 37.344380641763024 40.0 38.0 41.0 31.0 41.0 18-19 37.34658180306752 40.0 38.0 41.0 31.0 41.0 20-21 37.36250886206139 40.0 38.0 41.0 31.0 41.0 22-23 37.335941400555186 40.0 38.0 41.0 31.0 41.0 24-25 37.34730949064366 40.0 38.0 41.0 31.0 41.0 26-27 37.30071720288379 40.0 38.0 41.0 31.0 41.0 28-29 37.30244717712141 40.0 38.0 41.0 31.0 41.0 30-31 37.20924824755856 40.0 37.5 41.0 30.0 41.0 32-33 37.16445551994089 40.0 37.0 41.0 30.0 41.0 34-35 37.15559782717233 40.0 37.0 41.0 30.0 41.0 36-37 37.09715908523556 40.0 37.0 41.0 30.0 41.0 38-39 36.99859673626506 40.0 37.0 41.0 30.0 41.0 40-41 36.93522269985821 40.0 37.0 41.0 30.0 41.0 42-43 36.80766986449784 40.0 37.0 41.0 30.0 41.0 44-45 36.68400697481677 40.0 36.5 41.0 30.0 41.0 46-47 36.56876834821161 40.0 36.0 41.0 30.0 41.0 48-49 36.42554745072194 40.0 36.0 41.0 30.0 41.0 50-51 36.30193480019172 40.0 35.5 41.0 29.0 41.0 52-53 36.143956386176185 39.5 35.0 41.0 29.0 41.0 54-55 35.961376390469916 39.0 35.0 41.0 29.0 41.0 56-57 35.761408343818026 39.0 35.0 41.0 28.0 41.0 58-59 35.53520048229585 39.0 35.0 41.0 28.0 41.0 60-61 35.27620486589579 38.0 34.5 40.0 28.0 41.0 62-63 35.00059319643321 38.0 34.0 40.0 28.0 41.0 64-65 34.6520018273321 37.0 34.0 40.0 27.0 41.0 66-67 34.334139656102096 37.0 34.0 40.0 26.0 41.0 68-69 34.00101414434925 36.0 34.0 39.0 26.0 41.0 70-71 33.642066645198014 36.0 33.0 39.0 26.0 41.0 72-73 33.283226801370006 35.0 33.0 38.5 26.0 40.0 74-75 32.93323653715575 35.0 33.0 37.0 26.0 39.5 76-77 32.70109983174565 35.0 33.0 37.0 25.5 39.0 78-79 32.537144831046675 35.0 33.0 36.5 26.0 39.0 80-81 32.27533956673257 35.0 33.0 36.0 25.5 38.5 82-83 31.991896830627283 35.0 33.0 36.0 25.0 37.0 84-85 31.749811837217663 35.0 33.0 35.0 25.0 37.0 86-87 31.55202685329419 35.0 33.0 35.0 24.5 36.5 88-89 31.301467981746647 35.0 32.0 35.0 23.5 36.0 90-91 31.175895380244842 35.0 32.0 35.0 23.0 36.0 92-93 31.02745023515667 35.0 32.0 35.0 23.0 36.0 94-95 30.92799325484792 35.0 32.0 35.0 21.0 35.0 96-97 30.82844359734787 35.0 32.0 35.0 20.0 35.0 98-99 30.701698020889502 35.0 32.0 35.0 20.0 35.0 100-101 30.606775982066182 35.0 32.0 35.0 19.0 35.0 102-103 29.60122564805384 33.5 29.5 34.5 17.5 35.0 104-105 30.295304168414116 34.0 31.0 35.0 18.0 35.0 106-107 30.625700851756434 35.0 32.0 35.0 19.0 35.0 108-109 30.661389683562 35.0 32.0 35.0 18.5 35.0 110-111 30.58449757104627 35.0 32.0 35.0 18.0 35.0 112-113 30.485455609809677 35.0 32.0 35.0 16.0 35.0 114-115 30.38193612326004 35.0 32.0 35.0 8.5 35.0 116-117 30.251640417490464 35.0 32.0 35.0 3.5 35.0 118-119 30.08334862350968 35.0 32.0 35.0 2.0 35.0 120-121 29.901244183492103 35.0 32.0 35.0 2.0 35.0 122-123 29.720393537934612 35.0 32.0 35.0 2.0 35.0 124-125 28.91896830627284 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3084860415561259 1101 2 0.23262591285217482 1101 3 0.2838544009108688 1101 4 0.33060109614655886 1101 5 0.1909868221221913 1101 6 0.2771352979166579 1101 7 0.2888205624621918 1101 8 0.20884918090075644 1101 9 0.3904697498159919 1101 10-11 0.47232463266231406 1101 12-13 0.4836957755236213 1101 14-15 0.5773637612082752 1101 16-17 0.4884434375215534 1101 18-19 0.3553395506391368 1101 20-21 0.5086192354389354 1101 22-23 0.54253099995924 1101 24-25 0.5800342143767097 1101 26-27 0.552146524376866 1101 28-29 0.5451348741806328 1101 30-31 0.5686225388947221 1101 32-33 0.44296024465631945 1101 34-35 0.5445428225245905 1101 36-37 0.49788155807126344 1101 38-39 0.594588368401034 1101 40-41 0.4855254974210723 1101 42-43 0.5728641840553905 1101 44-45 0.5184592792816289 1101 46-47 0.5785537225492305 1101 48-49 0.520963698603218 1101 50-51 0.49352680227227097 1101 52-53 0.49260413905834355 1101 54-55 0.4882913053343074 1101 56-57 0.43710738396129045 1101 58-59 0.47628848465461715 1101 60-61 0.4466775400874923 1101 62-63 0.38810578140179786 1101 64-65 0.44689968402954605 1101 66-67 0.5349369555195125 1101 68-69 0.4811853822948464 1101 70-71 0.3308358119512178 1101 72-73 0.5258421997992926 1101 74-75 0.5215544728212649 1101 76-77 0.5937228929382954 1101 78-79 0.4537197447356789 1101 80-81 0.5960745206017748 1101 82-83 0.49965715430539603 1101 84-85 0.5813082114752994 1101 86-87 0.5315005432840856 1101 88-89 0.5472454897670573 1101 90-91 0.5100668960014083 1101 92-93 0.5467293105206359 1101 94-95 0.4685703504677363 1101 96-97 0.4557337957980643 1101 98-99 0.4526580499913315 1101 100-101 0.377031513248248 1101 102-103 0.1883002921023298 1101 104-105 0.3851857934119778 1101 106-107 0.45054717507818864 1101 108-109 0.46736759941347117 1101 110-111 0.5043391719936245 1101 112-113 0.4840768293072131 1101 114-115 0.42442332317075326 1101 116-117 0.4456960507192491 1101 118-119 0.48982067752456615 1101 120-121 0.401492751535379 1101 122-123 0.41798239971392803 1101 124-125 0.22820850136307058 1102 1 0.044317969698642656 1102 2 0.1005560525112088 1102 3 0.17262931426853356 1102 4 0.1270847512181632 1102 5 0.11244835756858151 1102 6 0.1959704489599261 1102 7 0.16908098989136278 1102 8 0.29437706647008355 1102 9 0.29418953821347316 1102 10-11 0.2568430451334862 1102 12-13 0.31839091666491015 1102 14-15 0.28583575842871767 1102 16-17 0.33383420234493144 1102 18-19 0.3482908585416524 1102 20-21 0.3570599398115277 1102 22-23 0.30597145506981605 1102 24-25 0.27795947915576136 1102 26-27 0.23437408575335184 1102 28-29 0.23015017430161322 1102 30-31 0.26352573960924275 1102 32-33 0.2860725453751485 1102 34-35 0.21228569220393467 1102 36-37 -0.026342904084103225 1102 38-39 0.0812085163066456 1102 40-41 -0.022514439300621802 1102 42-43 -0.0946321826177865 1102 44-45 0.08009197591224648 1102 46-47 0.0033373878577052096 1102 48-49 -0.015141808869501006 1102 50-51 -0.024862930872764366 1102 52-53 0.014643943347707022 1102 54-55 0.12042062703338985 1102 56-57 0.07092845265218983 1102 58-59 0.008255576741134973 1102 60-61 0.020523028844642965 1102 62-63 0.05181859807395739 1102 64-65 -0.053762097101653694 1102 66-67 -0.06545978968942023 1102 68-69 -0.0939440912447509 1102 70-71 -0.07760844297798997 1102 72-73 -0.014071213046719322 1102 74-75 -0.10261463794506653 1102 76-77 -0.031262503352159854 1102 78-79 -0.05084993198860843 1102 80-81 -0.11148643347426912 1102 82-83 -0.13349176319344735 1102 84-85 -0.09344082353820937 1102 86-87 -0.10990503569448862 1102 88-89 -0.08305708387274535 1102 90-91 -0.1491053562273983 1102 92-93 -0.16031656548692652 1102 94-95 -0.25022195034242145 1102 96-97 -0.21473868320208922 1102 98-99 -0.27517970289068927 1102 100-101 -0.29535904645723576 1102 102-103 -0.5042409131917687 1102 104-105 -0.43978812921481136 1102 106-107 -0.38443537848365494 1102 108-109 -0.2763010503132719 1102 110-111 -0.27478994839307447 1102 112-113 -0.3197536808959782 1102 114-115 -0.352584215958629 1102 116-117 -0.38003526690258127 1102 118-119 -0.355190961679515 1102 120-121 -0.5859012502692025 1102 122-123 -0.5888972318501828 1102 124-125 -0.598862552010587 1103 1 0.24988207303630006 1103 2 0.21316051143660175 1103 3 0.18122570140596395 1103 4 0.3356087336130855 1103 5 0.19173825476055129 1103 6 0.17287300089189017 1103 7 0.0545222733324664 1103 8 0.20178112180607854 1103 9 0.32120388172705105 1103 10-11 0.386020983140277 1103 12-13 0.3181210749161991 1103 14-15 0.4037105116324682 1103 16-17 0.3451692318264037 1103 18-19 0.3672158157189429 1103 20-21 0.42204319621481545 1103 22-23 0.46905475739649205 1103 24-25 0.47768762616420446 1103 26-27 0.49531021873673353 1103 28-29 0.3507887103849896 1103 30-31 0.44279368073346603 1103 32-33 0.4318348566954313 1103 34-35 0.397082037105271 1103 36-37 0.3900731952444758 1103 38-39 0.44625412123821206 1103 40-41 0.4131040011146325 1103 42-43 0.2780033500096053 1103 44-45 0.34322539170653954 1103 46-47 0.24675056753208224 1103 48-49 0.3641165788511884 1103 50-51 0.30462306249684445 1103 52-53 0.328814322433459 1103 54-55 0.26893834105476344 1103 56-57 0.15242820292287007 1103 58-59 0.14264933786581935 1103 60-61 0.09791900568853151 1103 62-63 0.05533324494999903 1103 64-65 0.08878138764474386 1103 66-67 0.12744270190525953 1103 68-69 -0.0020219129346656928 1103 70-71 -0.010039901497776782 1103 72-73 0.06506029877100872 1103 74-75 -0.010225683690784138 1103 76-77 0.029025339020037677 1103 78-79 0.02472587007827798 1103 80-81 0.06125502460974985 1103 82-83 0.09034783348532471 1103 84-85 -0.07850399332398084 1103 86-87 -0.014602293364863073 1103 88-89 0.1565255161949679 1103 90-91 0.09494387899751189 1103 92-93 0.05222571251376351 1103 94-95 -0.03804588614354998 1103 96-97 0.0359317310804137 1103 98-99 0.019146413432892473 1103 100-101 -0.026405596672940845 1103 102-103 -0.1545310349725213 1103 104-105 -0.007337396284395226 1103 106-107 -0.03401234707139267 1103 108-109 -0.004360904900416074 1103 110-111 -0.02138508185580079 1103 112-113 -0.08437038829030996 1103 114-115 -0.10256158947659344 1103 116-117 -0.07412941988442157 1103 118-119 -0.0499890496579809 1103 120-121 -0.28905856633266325 1103 122-123 -0.33818743072857416 1103 124-125 -0.4105339618582988 1104 1 0.3790576087252475 1104 2 0.36135123089168886 1104 3 0.29585604055737846 1104 4 0.43697101543514094 1104 5 0.36324385998263153 1104 6 0.3086045178494601 1104 7 0.33959393503248236 1104 8 0.44386995393322337 1104 9 0.612079431606432 1104 10-11 0.5373265032197381 1104 12-13 0.41244490065765405 1104 14-15 0.541520927412229 1104 16-17 0.43618668606224986 1104 18-19 0.4283482124835274 1104 20-21 0.4297131266816834 1104 22-23 0.5039916099578647 1104 24-25 0.4254952038568405 1104 26-27 0.5430468442511298 1104 28-29 0.46022535008982857 1104 30-31 0.5761128236311492 1104 32-33 0.573334785743242 1104 34-35 0.5007857408089791 1104 36-37 0.5059664831684145 1104 38-39 0.38173021035414934 1104 40-41 0.4445216256357014 1104 42-43 0.36569314708636824 1104 44-45 0.32909881302057187 1104 46-47 0.4338159854404111 1104 48-49 0.40158642417544144 1104 50-51 0.28924063025617386 1104 52-53 0.252079541675684 1104 54-55 0.23090797352248416 1104 56-57 0.27810579622499176 1104 58-59 0.22183906399041575 1104 60-61 0.15393575040259577 1104 62-63 0.2535878213654925 1104 64-65 0.3876461527930317 1104 66-67 0.34324375199749824 1104 68-69 0.2692424548814287 1104 70-71 0.015219073952764006 1104 72-73 0.15983112323532112 1104 74-75 0.05160913963829472 1104 76-77 0.15291493743070816 1104 78-79 0.24094553802208907 1104 80-81 0.2660159157691169 1104 82-83 0.25429703172570584 1104 84-85 0.07942136498452257 1104 86-87 0.06933413348344075 1104 88-89 0.08504829184418128 1104 90-91 0.025488791726335336 1104 92-93 0.05062928850354709 1104 94-95 0.1291387314242094 1104 96-97 0.02125172540424103 1104 98-99 0.06796860727424558 1104 100-101 -0.053480947886221486 1104 102-103 -0.07252450737212612 1104 104-105 -0.05835201243311161 1104 106-107 -0.03459415493289342 1104 108-109 9.534132491531011E-4 1104 110-111 -0.10418627385239532 1104 112-113 -0.07824012009107761 1104 114-115 -0.14283404584100268 1104 116-117 -0.1456634059777926 1104 118-119 -0.1077654083921935 1104 120-121 -0.26887976684351855 1104 122-123 -0.22477746904275264 1104 124-125 -0.3678136553448894 1105 1 0.1360453599565261 1105 2 0.07205220380179966 1105 3 -0.010038874075039672 1105 4 0.2017219732895441 1105 5 0.06795439240085699 1105 6 0.24698341632554843 1105 7 0.26463243956822424 1105 8 0.18579092358992 1105 9 0.2319858439528062 1105 10-11 0.20259835389482816 1105 12-13 0.31414688766474796 1105 14-15 0.4132215645974 1105 16-17 0.3743850891190732 1105 18-19 0.36352197609856063 1105 20-21 0.41627270747545 1105 22-23 0.4625262917286932 1105 24-25 0.3602271603718066 1105 26-27 0.2832973227457032 1105 28-29 0.3113038231989478 1105 30-31 0.4062576352963774 1105 32-33 0.5193811919288933 1105 34-35 0.3748670831549674 1105 36-37 0.21640043361264816 1105 38-39 0.2543483803417246 1105 40-41 0.2937018683959849 1105 42-43 0.3207563637466322 1105 44-45 0.2547601392379164 1105 46-47 0.22994528453672558 1105 48-49 0.045044633342456564 1105 50-51 0.09000656767288717 1105 52-53 0.12004619069084299 1105 54-55 0.09962394676139041 1105 56-57 0.13066751596734605 1105 58-59 0.04861606994694512 1105 60-61 0.08077367139993896 1105 62-63 0.15181955464067443 1105 64-65 0.13464677924885393 1105 66-67 0.04845994847619295 1105 68-69 -0.029960631019157802 1105 70-71 0.0721045502149309 1105 72-73 -0.05778907148514634 1105 74-75 -0.09929056859250807 1105 76-77 -0.06062100763499956 1105 78-79 0.11169853099262639 1105 80-81 0.07106200007414287 1105 82-83 0.09207145225407487 1105 84-85 0.036526792999211466 1105 86-87 0.06658170813032172 1105 88-89 0.04803357878517289 1105 90-91 0.08344962612394724 1105 92-93 0.055244641170485664 1105 94-95 -0.03834959607720023 1105 96-97 -0.0655411323072208 1105 98-99 -0.04130484242066146 1105 100-101 -0.13389555088282634 1105 102-103 -0.28243185421059636 1105 104-105 -0.11265206054215327 1105 106-107 -0.12597675330549052 1105 108-109 -0.09150229355567774 1105 110-111 -0.10502731674558419 1105 112-113 -0.11712433651944565 1105 114-115 -0.20883670395187792 1105 116-117 -0.2376473673244277 1105 118-119 -0.2054781524417315 1105 120-121 -0.2863693057235821 1105 122-123 -0.43938254229654916 1105 124-125 -0.5891050195995291 1106 1 0.15253815397526793 1106 2 0.27552675332980314 1106 3 0.18743449884966168 1106 4 0.22509589753286008 1106 5 0.17618725990018902 1106 6 0.1368042984638862 1106 7 0.23770086104923394 1106 8 0.2819700328840895 1106 9 0.3158159732792143 1106 10-11 0.357549976079504 1106 12-13 0.30054812366937966 1106 14-15 0.4135653355253055 1106 16-17 0.39293908078860795 1106 18-19 0.2873611869747137 1106 20-21 0.1471682539462833 1106 22-23 0.2719838476209162 1106 24-25 0.16253852901562027 1106 26-27 0.24962544227945926 1106 28-29 0.16581321960431694 1106 30-31 0.2514527315450934 1106 32-33 0.31238629244708704 1106 34-35 0.2544412355793497 1106 36-37 0.3221108863907318 1106 38-39 0.18618499526545662 1106 40-41 0.2112705618951054 1106 42-43 0.17648216662845329 1106 44-45 0.08183711536743488 1106 46-47 0.15388801936832408 1106 48-49 0.15566462046265173 1106 50-51 0.13258971882785886 1106 52-53 0.16991475096534003 1106 54-55 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0.915829625129426 2203 28-29 0.8757966196089626 2203 30-31 0.9429704537117587 2203 32-33 0.9426268470206622 2203 34-35 0.8634960824291369 2203 36-37 0.9355446724688292 2203 38-39 0.8901690668285838 2203 40-41 0.8463999023142037 2203 42-43 0.9303022005843147 2203 44-45 0.9117674738808219 2203 46-47 0.9352103354601411 2203 48-49 1.002140464707594 2203 50-51 0.9514575322799601 2203 52-53 0.9113287595008188 2203 54-55 0.9366164407416022 2203 56-57 0.9675883628860191 2203 58-59 1.0545669164623703 2203 60-61 1.042600909092485 2203 62-63 0.9472088682114261 2203 64-65 1.0178617089123208 2203 66-67 1.0631000714067085 2203 68-69 1.008882337343799 2203 70-71 1.0977790406927213 2203 72-73 1.1430858867507396 2203 74-75 1.163676829474248 2203 76-77 0.9872626317764954 2203 78-79 0.8795960749745646 2203 80-81 0.8680028865679148 2203 82-83 0.8173600566989307 2203 84-85 0.9223291176763304 2203 86-87 0.9129009213519801 2203 88-89 0.9707588025832266 2203 90-91 0.8493255708570651 2203 92-93 0.8678355501304722 2203 94-95 0.8954964037845485 2203 96-97 0.8911725213904873 2203 98-99 0.880245405571781 2203 100-101 0.9079567740823222 2203 102-103 0.9513739296874597 2203 104-105 0.9423658620242961 2203 106-107 0.9520452765457712 2203 108-109 1.0465128092128495 2203 110-111 1.0205996850517138 2203 112-113 1.0564916150687402 2203 114-115 1.1165779489163121 2203 116-117 1.016033455457464 2203 118-119 0.932581954762437 2203 120-121 0.9356161531632452 2203 122-123 0.8221049299087113 2203 124-125 0.985447168264109 2204 1 0.2610978355884903 2204 2 0.25161665247454224 2204 3 0.28060341333238625 2204 4 0.22015291965622907 2204 5 0.24872524331561863 2204 6 0.23712086307979519 2204 7 0.246870244088079 2204 8 0.27065888558681905 2204 9 0.3328669330961844 2204 10-11 0.317395873176487 2204 12-13 0.1915636322986245 2204 14-15 0.21855487978216104 2204 16-17 0.21557287236211664 2204 18-19 0.23826944831333918 2204 20-21 0.19154948509196146 2204 22-23 0.18384540374786695 2204 24-25 0.3651901372911226 2204 26-27 0.3419172601904492 2204 28-29 0.4241217753812947 2204 30-31 0.35982332762369396 2204 32-33 0.3607665199571102 2204 34-35 0.34217583902757553 2204 36-37 0.22887942545008855 2204 38-39 0.20539398865071234 2204 40-41 0.1994583198540596 2204 42-43 0.23630731131685678 2204 44-45 0.15010241647259193 2204 46-47 0.13338868894081202 2204 48-49 0.2616045816476884 2204 50-51 0.1739228043856258 2204 52-53 0.08668530018832143 2204 54-55 0.0922968799378836 2204 56-57 0.15389308739652563 2204 58-59 0.15977486085106563 2204 60-61 0.2741992090154781 2204 62-63 0.20527825399005195 2204 64-65 -2.805913553041897 2204 66-67 -0.5103441014247281 2204 68-69 0.13639285343443674 2204 70-71 0.06710808202197427 2204 72-73 0.16423693923493232 2204 74-75 0.19204912533665208 2204 76-77 0.16073947595750582 2204 78-79 0.19000488982135266 2204 80-81 0.24901098731236004 2204 82-83 0.16364427410341875 2204 84-85 0.26037512302043453 2204 86-87 0.263953843104094 2204 88-89 0.3492279456604024 2204 90-91 0.24029624540374428 2204 92-93 0.30801671568086775 2204 94-95 0.2834635749124068 2204 96-97 0.25627776980942585 2204 98-99 0.3135386046803177 2204 100-101 0.29454416410822404 2204 102-103 0.36589204186687496 2204 104-105 0.326056222225926 2204 106-107 0.33443315839216936 2204 108-109 0.32748750933653525 2204 110-111 0.488445753404843 2204 112-113 0.38195295202703505 2204 114-115 0.39415935016715764 2204 116-117 0.3784692066651374 2204 118-119 0.4016976842670026 2204 120-121 0.503165262285556 2204 122-123 0.5976808353718255 2204 124-125 0.7068167103984777 2205 1 0.30099192157633325 2205 2 0.12937920817188342 2205 3 0.24920220463442533 2205 4 0.39459670195816443 2205 5 0.22448093144361536 2205 6 0.21158375395079787 2205 7 0.28967451836189895 2205 8 0.44088132948283487 2205 9 0.4454424947117843 2205 10-11 0.5608717002968362 2205 12-13 0.4859807093856858 2205 14-15 0.5280948058956909 2205 16-17 0.4684309709047625 2205 18-19 0.5010515875950148 2205 20-21 0.5241074999311479 2205 22-23 0.5806255738753094 2205 24-25 0.4563922606094124 2205 26-27 0.48833936956055624 2205 28-29 0.521744072022571 2205 30-31 0.3959660524408619 2205 32-33 0.6131327797659978 2205 34-35 0.6197502059077848 2205 36-37 0.6311773298410301 2205 38-39 0.739964574841153 2205 40-41 0.7532058485151083 2205 42-43 0.6933989475213664 2205 44-45 0.8082230365258667 2205 46-47 0.7531411196155631 2205 48-49 0.656286857604222 2205 50-51 0.7308654405332788 2205 52-53 0.653208490650016 2205 54-55 0.6555351778286038 2205 56-57 0.6987265225702615 2205 58-59 0.7249082717702322 2205 60-61 0.7731169298921827 2205 62-63 0.7197709880556289 2205 64-65 0.7414586048733298 2205 66-67 0.7442240521117256 2205 68-69 0.7204277339501033 2205 70-71 0.7584086090730153 2205 72-73 0.9044766348221103 2205 74-75 0.8275679247859244 2205 76-77 0.7040290215190268 2205 78-79 0.6762457304832452 2205 80-81 0.5666128582967644 2205 82-83 0.611555790273016 2205 84-85 0.6984765841242329 2205 86-87 0.704283827579733 2205 88-89 0.7586060067200222 2205 90-91 0.70682710088467 2205 92-93 0.6782286876492094 2205 94-95 0.48739734914628485 2205 96-97 0.5706993775346128 2205 98-99 0.7262898139900429 2205 100-101 0.6302819024165522 2205 102-103 0.6140955304942182 2205 104-105 0.6421987707116728 2205 106-107 0.6163970982552343 2205 108-109 0.7055075449051031 2205 110-111 0.6972501435536032 2205 112-113 0.723111802173392 2205 114-115 0.7531993766816463 2205 116-117 0.8552629111293442 2205 118-119 0.9235689889540346 2205 120-121 0.9622942462062838 2205 122-123 0.9852023460067727 2205 124-125 1.074379932412704 2206 1 0.41668688557364675 2206 2 0.35404915863476916 2206 3 0.37814459230722264 2206 4 0.40564154605471714 2206 5 0.2827711331334868 2206 6 0.28735194479296666 2206 7 0.3543619896936008 2206 8 0.42665023612492803 2206 9 0.4151299908146058 2206 10-11 0.45914593913586543 2206 12-13 0.505121694808139 2206 14-15 0.647516627474964 2206 16-17 0.569285386595908 2206 18-19 0.5075282956533655 2206 20-21 0.5640589587877756 2206 22-23 0.6078633839648759 2206 24-25 0.667282806259081 2206 26-27 0.7264734482824977 2206 28-29 0.7304617634362671 2206 30-31 0.658315010541962 2206 32-33 0.6935365865915912 2206 34-35 0.7545625800555484 2206 36-37 0.7418275273010195 2206 38-39 0.6202975191825004 2206 40-41 0.5978461727195139 2206 42-43 0.6883895438920362 2206 44-45 0.6966991019018067 2206 46-47 0.6426412572234028 2206 48-49 0.7373769952650235 2206 50-51 0.7640414003842579 2206 52-53 0.7345357477132382 2206 54-55 0.6818031558954019 2206 56-57 0.6275579868799497 2206 58-59 0.6690827110161592 2206 60-61 0.6661220954392775 2206 62-63 0.5918115013063883 2206 64-65 0.6547827506839994 2206 66-67 0.7466919389183033 2206 68-69 0.7277653665247072 2206 70-71 0.7005182474129725 2206 72-73 0.6726311744884441 2206 74-75 0.6928817979280737 2206 76-77 0.7161121171777367 2206 78-79 0.6192195392102278 2206 80-81 0.6473755046791325 2206 82-83 0.6708552015233096 2206 84-85 0.5448957309351421 2206 86-87 0.6321117198940449 2206 88-89 0.789077985373801 2206 90-91 0.6854686732118083 2206 92-93 0.7001989619645848 2206 94-95 0.6586291622333285 2206 96-97 0.7071044492247971 2206 98-99 0.761210600221947 2206 100-101 0.7531476783137734 2206 102-103 0.7586433815483815 2206 104-105 0.7671394267978258 2206 106-107 0.8399683214582225 2206 108-109 0.9188782775032607 2206 110-111 0.9059229052822566 2206 112-113 0.8810670641655598 2206 114-115 0.9733401415557204 2206 116-117 1.0283795501292339 2206 118-119 0.9667938230772215 2206 120-121 0.905218768947968 2206 122-123 0.853111634510185 2206 124-125 1.0041919059516786 2207 1 0.21686556022633852 2207 2 0.2453225624021691 2207 3 0.1695393062242374 2207 4 0.12300755533073726 2207 5 0.13041664425087163 2207 6 0.23290265080717631 2207 7 0.2465708317651547 2207 8 0.16974478476704036 2207 9 0.217399535453751 2207 10-11 0.3076281714054474 2207 12-13 0.2531003678325092 2207 14-15 0.32132369345194434 2207 16-17 0.3035286365138177 2207 18-19 0.34008178610188367 2207 20-21 0.24921315430065505 2207 22-23 0.3069342384551135 2207 24-25 0.34914049344172327 2207 26-27 0.4415490330544074 2207 28-29 0.41978565610800445 2207 30-31 0.5084411717406354 2207 32-33 0.45339354509917484 2207 34-35 0.44917572755187507 2207 36-37 0.40450440401244947 2207 38-39 0.5359712756177757 2207 40-41 0.487517627789984 2207 42-43 0.538523677094382 2207 44-45 0.517657482614851 2207 46-47 0.43428378172289683 2207 48-49 0.43036487171320914 2207 50-51 0.4759515908439056 2207 52-53 0.5174313728133129 2207 54-55 0.5767803186491136 2207 56-57 0.4813358346153507 2207 58-59 0.5044884826111229 2207 60-61 0.5277817953662733 2207 62-63 0.4857104081581056 2207 64-65 0.5512521198712221 2207 66-67 0.49041228984929575 2207 68-69 0.482489202747864 2207 70-71 0.6187263377415348 2207 72-73 0.6112698988864409 2207 74-75 0.555443860824802 2207 76-77 0.5305908702613777 2207 78-79 0.5292854999079069 2207 80-81 0.6135683572859421 2207 82-83 0.5471425105005281 2207 84-85 0.6232217696024129 2207 86-87 0.6063107540264276 2207 88-89 0.6201221380617632 2207 90-91 0.592402993400043 2207 92-93 0.6197992694464638 2207 94-95 0.6286288162144444 2207 96-97 0.6886005805979885 2207 98-99 0.6698803577644803 2207 100-101 0.759814478412558 2207 102-103 0.7373026669311891 2207 104-105 0.7752600998656121 2207 106-107 0.8127361265043973 2207 108-109 0.8046564264786404 2207 110-111 0.8100335248053234 2207 112-113 0.9047436606695491 2207 114-115 0.8309422612266921 2207 116-117 0.9413675894098397 2207 118-119 0.9671603656294074 2207 120-121 1.096447207222269 2207 122-123 1.1374190675385698 2207 124-125 1.2279399282162977 2208 1 0.22284319719938495 2208 2 0.27710565407386056 2208 3 0.3225464073113855 2208 4 0.2928665736060978 2208 5 0.225441736338567 2208 6 0.278484580554597 2208 7 0.24612405174087826 2208 8 0.2802440988815391 2208 9 0.17606111085771659 2208 10-11 0.3824319831384386 2208 12-13 0.3106181534623502 2208 14-15 0.31017764262532665 2208 16-17 0.3494651927630059 2208 18-19 0.49362387129913543 2208 20-21 0.428652354039464 2208 22-23 0.40797687328230126 2208 24-25 0.3779703938369323 2208 26-27 0.47464852133905566 2208 28-29 0.28401455492605976 2208 30-31 0.23305567380881342 2208 32-33 0.2398736689008487 2208 34-35 0.2954541898255343 2208 36-37 0.28242089843979556 2208 38-39 0.452891337044683 2208 40-41 0.35975442087372755 2208 42-43 0.3254571633147947 2208 44-45 0.23712463449174948 2208 46-47 0.35571023268011004 2208 48-49 0.3420035489415483 2208 50-51 0.2661143175086522 2208 52-53 0.3151967650845364 2208 54-55 0.4181459285342939 2208 56-57 0.41884258156967746 2208 58-59 0.3136396894273261 2208 60-61 0.47956534451438415 2208 62-63 0.5085791275664207 2208 64-65 0.5512153407421465 2208 66-67 0.5337521737621813 2208 68-69 0.5350223056096226 2208 70-71 0.5381385759926189 2208 72-73 0.5562920124406432 2208 74-75 0.4916376669232605 2208 76-77 0.429198463448337 2208 78-79 0.31227876480188854 2208 80-81 0.36090191156831963 2208 82-83 0.4132162076471957 2208 84-85 0.4149706887732485 2208 86-87 0.4881589126674051 2208 88-89 0.4860426299772662 2208 90-91 0.3589802601792229 2208 92-93 0.41944615689541465 2208 94-95 0.32156850699988127 2208 96-97 0.4086269891645813 2208 98-99 0.3612127101016256 2208 100-101 0.46927597930381637 2208 102-103 0.49290839861699354 2208 104-105 0.4029634069922423 2208 106-107 0.5364037511288728 2208 108-109 0.4744483043304619 2208 110-111 0.5737266331699651 2208 112-113 0.5859157580310637 2208 114-115 0.5417486275869656 2208 116-117 0.582671275419095 2208 118-119 0.583753495979245 2208 120-121 0.6970889742265669 2208 122-123 0.6011072884753261 2208 124-125 0.5742146878930754 2209 1 0.07261566488111271 2209 2 0.10405684730622511 2209 3 0.041553269196704434 2209 4 0.11680557836829308 2209 5 0.016872628478381557 2209 6 0.06899318731481685 2209 7 0.15066198944951736 2209 8 0.05264689541752432 2209 9 0.25104739577001567 2209 10-11 0.05218561674865185 2209 12-13 0.031937990886262924 2209 14-15 0.1463171947280557 2209 16-17 0.14687799440613958 2209 18-19 0.08479553841441856 2209 20-21 0.05266524266979644 2209 22-23 0.18166340202715503 2209 24-25 0.04915568526379843 2209 26-27 0.06016602021225026 2209 28-29 0.0645508918175608 2209 30-31 0.02921497563783504 2209 32-33 0.10713056453120373 2209 34-35 -0.02400994689750746 2209 36-37 -0.022931182661956484 2209 38-39 -0.10831955324803744 2209 40-41 0.057791367543941874 2209 42-43 0.06340079213361349 2209 44-45 -0.08853580124940663 2209 46-47 -0.07855246393899051 2209 48-49 -0.036104329399506696 2209 50-51 0.045368343034894565 2209 52-53 0.08075654194892934 2209 54-55 -0.05316850179030297 2209 56-57 0.0945695996214937 2209 58-59 0.08564370252118891 2209 60-61 0.18395330428556633 2209 62-63 0.026303125536195182 2209 64-65 0.2407516168531174 2209 66-67 0.09934786639581716 2209 68-69 0.14799397635595568 2209 70-71 0.18090123274916436 2209 72-73 0.23734269472185332 2209 74-75 0.3324261404083444 2209 76-77 0.3026623933597037 2209 78-79 0.3107545153427935 2209 80-81 0.2939568121763614 2209 82-83 0.31585858209790274 2209 84-85 0.2695481338280352 2209 86-87 0.22915064572561405 2209 88-89 0.3713016142477379 2209 90-91 0.31563584689686053 2209 92-93 0.34609715744559466 2209 94-95 0.4428502184533798 2209 96-97 0.3171551293671122 2209 98-99 0.3591183866086851 2209 100-101 0.42782168271218524 2209 102-103 0.47115899280590057 2209 104-105 0.5711666634079435 2209 106-107 0.5237476655372539 2209 108-109 0.5479568329442799 2209 110-111 0.4879666436526371 2209 112-113 0.42142453450315287 2209 114-115 0.45321136241097904 2209 116-117 0.5609151600686957 2209 118-119 0.5768836381504379 2209 120-121 0.5826715161631633 2209 122-123 0.7117354355452683 2209 124-125 0.7450573981364883 2210 1 0.11355419222000762 2210 2 0.11525298588633959 2210 3 0.17373983119162517 2210 4 0.18308082547937232 2210 5 0.10096516182051118 2210 6 0.017175175418302047 2210 7 0.1232824114349782 2210 8 0.1555583532354703 2210 9 0.1935801881527226 2210 10-11 0.2082553685930506 2210 12-13 0.22538666104932048 2210 14-15 0.14503573548076787 2210 16-17 0.17664842023982885 2210 18-19 0.2739968666021255 2210 20-21 0.3410271078499463 2210 22-23 0.3638802000832442 2210 24-25 0.32690469297124736 2210 26-27 0.3481595551926304 2210 28-29 0.25940471420543787 2210 30-31 0.3223662329082373 2210 32-33 0.3883066923362861 2210 34-35 0.17944246154219456 2210 36-37 0.18705716271082196 2210 38-39 0.35703981608269686 2210 40-41 0.3437568874392696 2210 42-43 0.30130141994418835 2210 44-45 0.23586195138662447 2210 46-47 0.40131581678359396 2210 48-49 0.41142730851541387 2210 50-51 0.38125689841086796 2210 52-53 0.3862050801728145 2210 54-55 0.47956835651802265 2210 56-57 0.5565562737647198 2210 58-59 0.5298917041924227 2210 60-61 0.5903232177554543 2210 62-63 0.5671998721360083 2210 64-65 0.5821117998087715 2210 66-67 0.5739638684188861 2210 68-69 0.530746777314711 2210 70-71 0.5162840212836528 2210 72-73 0.49163401642254456 2210 74-75 0.3611690899086213 2210 76-77 0.3666908262521318 2210 78-79 0.4318279504891933 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2216 42-43 -0.7109717959252819 2216 44-45 -0.7164196839522532 2216 46-47 -0.6085288317444224 2216 48-49 -0.6396337081150847 2216 50-51 -0.6881342431629207 2216 52-53 -0.6524159660484372 2216 54-55 -0.6329318989270476 2216 56-57 -0.7216755150455612 2216 58-59 -0.771918073018611 2216 60-61 -0.8277191738643168 2216 62-63 -0.9206090631280759 2216 64-65 -0.7184577405127186 2216 66-67 -0.8557748419309235 2216 68-69 -0.7262458781287222 2216 70-71 -0.6948664624271288 2216 72-73 -0.6168747565828312 2216 74-75 -0.5582835628373104 2216 76-77 -0.6706722041029138 2216 78-79 -0.7563461690427431 2216 80-81 -0.7201118932499142 2216 82-83 -0.7503579140868908 2216 84-85 -0.7429344405010134 2216 86-87 -0.7073426596564154 2216 88-89 -0.730244837236036 2216 90-91 -0.8573339088101584 2216 92-93 -0.8036884672976292 2216 94-95 -0.7438311261587955 2216 96-97 -0.8116108346543633 2216 98-99 -0.8756732603059589 2216 100-101 -0.8379098794355713 2216 102-103 -0.7948264486385774 2216 104-105 -0.9354489464817632 2216 106-107 -0.8018253186154816 2216 108-109 -0.7289401510539513 2216 110-111 -0.740660968442274 2216 112-113 -0.7161109752394204 2216 114-115 -0.6998374716053384 2216 116-117 -0.6060710503877615 2216 118-119 -0.5809464137139777 2216 120-121 -0.4005907098982675 2216 122-123 -0.39945913150801005 2216 124-125 -0.3164045673738478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27949.0 3 5038.0 4 3139.0 5 2687.0 6 2852.0 7 3293.0 8 3621.0 9 4133.0 10 4687.0 11 5326.0 12 5844.0 13 5794.0 14 5469.0 15 5272.0 16 4873.0 17 4607.0 18 4306.0 19 4317.0 20 4334.0 21 4537.0 22 5340.0 23 7126.0 24 10462.0 25 14599.0 26 17289.0 27 18167.0 28 21617.0 29 25994.0 30 30820.0 31 37763.0 32 48783.0 33 64709.0 34 90544.0 35 136452.0 36 266447.0 37 492581.0 38 200938.0 39 627.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49778420435517 18.137932498460994 12.669556129119265 24.694727168064574 2 15.945827190760738 19.4938728228455 39.18509854538056 25.3752014410132 3 18.7950019128836 23.717072969800153 29.39687060980732 28.09105450750893 4 12.633578781679125 15.967415577461027 37.07046019380392 34.32854544705593 5 13.406735266137149 38.13768917498358 34.87055824254848 13.585017316330786 6 33.437313503646905 36.29933803938803 16.96233491561313 13.301013541351939 7 29.162903820721482 32.21915646927614 20.811538992029526 17.80640071797285 8 26.676489824855707 35.25484043296787 19.703108461645996 18.365561280530425 9 26.20218231382182 16.13044954366625 19.265871827132386 38.401496315379546 10-11 25.00127158218212 26.425162785922662 27.845028612939434 20.72853701895578 12-13 25.4939235098042 23.688357466004046 27.673321795917555 23.1443972282742 14-15 22.475103489724045 25.61415136554012 24.725135162291483 27.185609982444348 16-17 22.962441039134163 28.68715261612123 25.711399745621076 22.63900659912353 18-19 23.066923250467834 27.670141490343315 26.724989866354456 22.537945392834395 20-21 23.3331429860619 27.062670122873843 26.866050752823455 22.738136138240797 22-23 23.34366824436323 26.765266460966984 26.705197911049865 23.185867383619915 24-25 23.211704661194656 27.087857977353064 26.62584501627624 23.074592345176043 26-27 22.88260569731049 27.382164154187482 26.458730540118015 23.276499608384015 28-29 23.085439507717656 27.445427213140842 26.429096333002043 23.040036946139455 30-31 23.112683400376138 27.02906290790094 26.496527410475153 23.361726281247766 32-33 22.694942068918174 27.184265462590535 26.557679885916492 23.563112582574806 34-35 23.547086465373564 27.144612183494782 26.220641462226574 23.08765988890508 36-37 23.537789249931663 26.885593141005558 26.170511266689715 23.40610634237306 38-39 23.010644781099078 27.04656861597875 26.458676387483386 23.484110215438786 40-41 23.353592379095563 26.750552866935294 26.52250988326683 23.37334487070231 42-43 23.007492847906295 26.804811499268567 26.494918643373893 23.692777009451248 44-45 23.104630505963385 26.9911837183533 26.622717878675257 23.281467897008056 46-47 23.46112908314145 27.40399872561021 26.054967889059437 23.0799043021889 48-49 23.34825529726599 27.02491861881653 26.23588311065844 23.39094297325904 50-51 23.108854821959937 27.206809308409724 26.45266036586584 23.231675503764503 52-53 23.270185511678342 26.93083488056418 26.378450064749657 23.420529543007817 54-55 23.005271179268775 27.00733571408065 26.488736801768457 23.49865630488211 56-57 23.019950896629542 27.339680740719473 26.491979824431112 23.148388538219873 58-59 23.035540876876336 26.901987363354728 26.359275652115304 23.703196107653632 60-61 22.754796712145474 27.046476003723345 26.685624576590904 23.513102707540277 62-63 22.869961733718732 27.06680201170237 26.743927338064168 23.319308916514725 64-65 22.855221523924836 27.011065274509928 26.44775355580381 23.685959645761432 66-67 22.715262931409754 27.17419219548515 26.41637081415636 23.694174058948736 68-69 22.91079618822228 26.72616250295669 26.823678756185647 23.539362552635374 70-71 22.786085580247008 26.676752112108215 26.648012337195887 23.889149970448894 72-73 22.847651120641878 26.816892758890994 26.43063453943591 23.90482158103122 74-75 22.849040259425777 27.15532843872714 26.3310487122326 23.66458258961448 76-77 22.99897585808551 26.828087076402486 26.542375822012747 23.63056124349926 78-79 22.972811044089607 26.848415251777908 26.66299487301107 23.515778831121416 80-81 23.054398031852582 26.891898554532524 26.613926440365493 23.439776973249398 82-83 22.835077619948244 27.004733429797234 26.53460452660553 23.625584423648988 84-85 22.90234573806981 26.982712378805505 26.656749867302743 23.458192015821943 86-87 23.079049810820297 26.74013340094395 26.62464406911885 23.5561727191169 88-89 22.850882065479926 26.947746373564375 26.522098133763393 23.679273427192307 90-91 22.874151119260354 27.082084501293586 26.522555739905094 23.52120863954097 92-93 23.01999847720995 26.553277056671504 26.755889265703146 23.670835200415397 94-95 22.652971558810826 26.58504147725429 26.890379224176886 23.871607739758 96-97 22.87843592963256 26.360329819288385 26.908655634412842 23.852578616666207 98-99 22.728635803796298 26.500343719390173 26.569181211421284 24.20183926539225 100-101 22.626784222019598 26.376466711476997 26.870347729432964 24.12640133707044 102-103 22.522375493896003 26.104358017887915 27.07286538006667 24.300401108149416 104-105 22.388928310869048 26.403479702256917 26.89139691734677 24.316195069527264 106-107 22.4775328900764 26.397348447258857 26.63667099188569 24.488447670779056 108-109 22.439588818899704 26.291868432532763 26.69250850802372 24.576034240543816 110-111 22.576606634550515 26.508994207733966 26.73925566167325 24.175143496042274 112-113 22.558801520567037 26.564644382408503 26.977926064893087 23.898628032131374 114-115 22.35898143218858 26.748607773004746 26.706386925205127 24.18602386960155 116-117 22.204816795751135 26.64705955776348 26.949872371763266 24.19825127472212 118-119 22.099403360064336 26.813957728222892 26.641790019704853 24.444848892007922 120-121 22.076635648768413 27.034911753939028 26.743863440106182 24.144589157186374 122-123 22.105626460632624 27.033479907592994 26.689028837089126 24.171864794685256 124-125 22.053335521866977 27.067657206979632 26.72839288506048 24.150614386092908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 449.0 1 412.0 2 341.0 3 278.5 4 217.0 5 184.0 6 161.5 7 148.5 8 178.0 9 187.0 10 159.0 11 145.5 12 149.0 13 161.5 14 186.5 15 203.5 16 224.5 17 287.5 18 398.5 19 504.5 20 611.0 21 864.5 22 1249.0 23 1792.0 24 2665.5 25 3865.0 26 5445.0 27 7548.5 28 9875.5 29 12439.5 30 15503.5 31 19101.5 32 23645.5 33 26260.5 34 27561.5 35 30349.5 36 34262.0 37 38211.5 38 41306.0 39 43826.5 40 46008.5 41 49437.5 42 51937.5 43 53513.5 44 56458.0 45 59908.0 46 60896.5 47 60310.5 48 59568.0 49 58794.5 50 58612.5 51 57873.0 52 58961.0 53 61505.0 54 63942.5 55 61071.0 56 54342.0 57 49796.0 58 47125.0 59 41265.5 60 32401.0 61 26263.0 62 21685.0 63 16564.0 64 11756.5 65 8594.5 66 6130.5 67 4783.5 68 3739.5 69 2471.5 70 1644.0 71 1094.5 72 769.5 73 586.0 74 434.0 75 314.5 76 206.0 77 107.0 78 71.0 79 55.5 80 41.0 81 27.0 82 23.5 83 18.5 84 16.0 85 14.5 86 14.5 87 11.0 88 7.5 89 7.5 90 5.0 91 1.5 92 2.0 93 3.5 94 2.0 95 1.5 96 1.0 97 0.5 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04081540950212689 2 0.008113154794000758 3 0.0026835819703233278 4 0.006116070537015957 5 0.023715375551694527 6 0.026711001937171727 7 0.0029332175024464283 8 0.0 9 0.0 10-11 9.36133245461626E-5 12-13 8.11315479400076E-4 14-15 1.872266490923252E-4 16-17 1.2481776606155013E-4 18-19 3.120444151538753E-5 20-21 9.36133245461626E-5 22-23 0.0 24-25 0.0 26-27 6.240888303077506E-5 28-29 2.1843109060771274E-4 30-31 9.36133245461626E-5 32-33 6.240888303077506E-5 34-35 7.801110378846883E-4 36-37 1.2481776606155013E-4 38-39 9.36133245461626E-5 40-41 4.05657739700038E-4 42-43 4.992710642462005E-4 44-45 0.0010297465700077886 46-47 3.120444151538753E-5 48-49 0.0 50-51 0.0 52-53 6.864977133385257E-4 54-55 0.0023715375551694525 56-57 1.2481776606155013E-4 58-59 7.489065963693008E-4 60-61 9.049288039462384E-4 62-63 7.177021548539133E-4 64-65 0.001622630958800152 66-67 0.0028396041779002656 68-69 0.0024339464382002276 70-71 0.001685039841830927 72-73 0.0024963553212310028 74-75 0.0017474487248617019 76-77 0.0011857687775847262 78-79 0.0019346753739540273 80-81 0.0010609510115231762 82-83 5.928843887923631E-4 84-85 2.808399736384878E-4 86-87 0.0014666087512232142 88-89 0.0046182573442773555 90-91 3.4324885666926286E-4 92-93 8.737243624308509E-4 94-95 7.489065963693008E-4 96-97 2.4963553212310025E-4 98-99 7.177021548539133E-4 100-101 0.001685039841830927 102-103 0.0015602220757693767 104-105 9.36133245461626E-4 106-107 0.006552932718231382 108-109 0.0015602220757693767 110-111 0.001685039841830927 112-113 0.0024339464382002276 114-115 0.0029332175024464283 116-117 0.001622630958800152 118-119 0.043686218121542546 120-121 0.012263345515547301 122-123 0.0047742795518542925 124-125 0.0028396041779002656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1602336.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.9249972438849 #Duplication Level Percentage of deduplicated Percentage of total 1 86.6704649913004 66.67125280582007 2 8.83221015210005 13.58835483215417 3 2.288779827003896 5.281931456524022 4 0.8629384736349598 2.655261588240461 5 0.39462140018696745 1.5178125060880236 6 0.23048951486508296 1.0638243177444524 7 0.15872771423633886 0.8547090286110961 8 0.09432229773029491 0.5804593994351861 9 0.07173065518017709 0.4966092406833463 >10 0.363205572800473 5.078816473787019 >50 0.024236170497792274 1.2653191259742167 >100 0.008273230463625492 0.9456492249380279 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.240888303077506E-5 7 0.0 0.0 0.0 0.0 6.240888303077506E-5 8 0.0 0.0 0.0 0.0 6.240888303077506E-5 9 0.0 0.0 0.0 6.240888303077506E-5 6.240888303077506E-5 10-11 3.120444151538753E-5 0.0 0.0 6.240888303077506E-5 1.2481776606155013E-4 12-13 6.240888303077506E-5 0.0 0.0 1.2481776606155013E-4 1.2481776606155013E-4 14-15 6.240888303077506E-5 0.0 0.0 1.2481776606155013E-4 1.8722664909232522E-4 16-17 6.240888303077506E-5 0.0 0.0 1.872266490923252E-4 2.4963553212310025E-4 18-19 6.240888303077506E-5 0.0 0.0 2.4963553212310025E-4 2.4963553212310025E-4 20-21 6.240888303077506E-5 0.0 0.0 2.4963553212310025E-4 2.808399736384878E-4 22-23 6.240888303077506E-5 0.0 0.0 2.808399736384878E-4 4.6806662273081304E-4 24-25 1.2481776606155013E-4 0.0 0.0 3.7445329818465043E-4 6.240888303077507E-4 26-27 1.2481776606155013E-4 0.0 0.0 8.113154794000758E-4 6.240888303077507E-4 28-29 1.2481776606155013E-4 0.0 0.0 0.0034324885666926286 6.240888303077507E-4 30-31 1.2481776606155013E-4 0.0 0.0 0.01064071455674715 6.240888303077507E-4 32-33 1.2481776606155013E-4 0.0 0.0 0.026867024144748668 6.240888303077507E-4 34-35 1.2481776606155013E-4 0.0 0.0 0.05198659956463563 6.240888303077507E-4 36-37 1.2481776606155013E-4 0.0 0.0 0.09433102670101652 6.240888303077507E-4 38-39 1.2481776606155013E-4 0.0 0.0 0.1599539672078765 6.240888303077507E-4 40-41 1.2481776606155013E-4 0.0 0.0 0.229321440696583 6.240888303077507E-4 42-43 1.2481776606155013E-4 0.0 0.0 0.29719110099255086 6.552932718231382E-4 44-45 1.2481776606155013E-4 0.0 0.0 0.3828472929522897 6.864977133385257E-4 46-47 1.8722664909232522E-4 0.0 0.0 0.4851042477982146 6.864977133385257E-4 48-49 1.8722664909232522E-4 0.0 0.0 0.5985323927066483 7.489065963693009E-4 50-51 1.8722664909232522E-4 0.0 0.0 0.7187943103069518 7.489065963693009E-4 52-53 1.8722664909232522E-4 0.0 0.0 0.8375896191560321 7.801110378846884E-4 54-55 1.8722664909232522E-4 0.0 0.0 0.9684298424300521 8.425199209154634E-4 56-57 1.8722664909232522E-4 0.0 0.0 1.1183047750284585 8.737243624308509E-4 58-59 1.8722664909232522E-4 0.0 0.0 1.2848116749545664 8.737243624308509E-4 60-61 1.8722664909232522E-4 0.0 0.0 1.470384488646576 8.737243624308509E-4 62-63 1.8722664909232522E-4 0.0 0.0 1.6470640365067002 9.36133245461626E-4 64-65 1.8722664909232522E-4 0.0 0.0 1.8283306372695864 9.36133245461626E-4 66-67 1.8722664909232522E-4 0.0 0.0 2.0434852615181835 9.673376869770134E-4 68-69 1.8722664909232522E-4 0.0 0.0 2.29321440696583 9.98542128492401E-4 70-71 1.8722664909232522E-4 0.0 0.0 2.5544579913326544 9.98542128492401E-4 72-73 1.8722664909232522E-4 0.0 0.0 2.820881512991033 0.0011857687775847262 74-75 1.8722664909232522E-4 0.0 0.0 3.0865249236115275 0.0011857687775847262 76-77 1.8722664909232522E-4 0.0 0.0 3.3778183851576697 0.0011857687775847262 78-79 1.8722664909232522E-4 0.0 0.0 3.7126108381762624 0.0011857687775847262 80-81 1.8722664909232522E-4 0.0 0.0 4.073458999860204 0.0011857687775847262 82-83 1.8722664909232522E-4 0.0 0.0 4.446820142591816 0.0011857687775847262 84-85 2.4963553212310025E-4 0.0 0.0 4.817684930002196 0.0011857687775847262 86-87 3.1204441515387534E-4 0.0 0.0 5.1956643300780865 0.0011857687775847262 88-89 3.1204441515387534E-4 0.0 0.0 5.609840882311825 0.0011857687775847262 90-91 3.1204441515387534E-4 0.0 0.0 6.066829928304675 0.0011857687775847262 92-93 3.7445329818465043E-4 0.0 0.0 6.529342160445749 0.0012481776606155014 94-95 3.7445329818465043E-4 0.0 0.0 6.9964102410480695 0.0013105865436462765 96-97 3.7445329818465043E-4 0.0 0.0 7.47227797415773 0.0013105865436462765 98-99 3.7445329818465043E-4 0.0 0.0 7.969707976354522 0.0013105865436462765 100-101 3.7445329818465043E-4 0.0 0.0 8.523087542188405 0.0013105865436462765 102-103 3.7445329818465043E-4 0.0 0.0 9.113943642282269 0.0013729954266770514 104-105 4.3686218121542547E-4 0.0 0.0 9.690788948135722 0.0014354043097078266 106-107 4.3686218121542547E-4 0.0 0.0 10.26030121023306 0.0014354043097078266 108-109 4.3686218121542547E-4 0.0 0.0 10.855182683282408 0.0014354043097078266 110-111 4.3686218121542547E-4 0.0 0.0 11.487821530565373 0.0014354043097078266 112-113 4.3686218121542547E-4 0.0 0.0 12.156407894474068 0.0014354043097078266 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1430 0.0 46.634865 1 GTATCAA 2055 0.0 44.041336 1 ATCAACG 2120 0.0 40.99048 3 CAACGCA 2250 0.0 38.619724 5 TCAACGC 2260 0.0 38.451244 4 TATCAAC 2540 0.0 35.38529 2 AACGCAG 2470 0.0 35.17332 6 GTACATG 5210 0.0 33.142784 1 TACATGG 5575 0.0 30.535194 2 ACATGGG 5455 0.0 29.8966 3 CGCAGAG 3005 0.0 29.101032 8 ACGCAGA 3010 0.0 28.856855 7 CATGGGG 3585 0.0 27.394335 4 GAGTACA 3955 0.0 24.088083 1 TAAGGTG 1020 0.0 22.756386 5 GTAAGGT 830 0.0 22.230518 4 GCAGAGT 3945 0.0 21.262173 9 ATGGGGA 2305 0.0 21.17297 5 GTGTAGC 1135 0.0 20.98422 1 GTATAGG 530 0.0 20.222057 1 >>END_MODULE