FastQCFastQC Report
Thu 26 May 2016
SRR1364373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602336
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.17755327222255507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27750.17318465041040082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.069.225111
GTATCAA42100.048.9519731
ATCAACG49750.040.8082123
TCAACGC51450.039.459844
CAACGCA51600.039.3451275
TATCAAC52400.038.858062
AACGCAG53900.037.64746
GTACATG52750.035.0038151
TACATGG55550.032.689032
ACATGGG54100.032.4646723
ACGCAGA63750.031.9188697
TGGTATC12000.031.2568782
CGCAGAG65050.031.189528
GTGGTAT12050.030.6464941
CATGGGG36050.028.2408084
GCAGAGT74950.027.0697569
CAGAGTA72450.024.47256510-11
GAGTACT49250.024.46360412-13
TAAGGTG10550.024.2662285
GTACTTT50000.023.68016214-15
Produced by FastQC (version 0.11.5)