FastQCFastQC Report
Thu 26 May 2016
SRR1364372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509815
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA17320.11471604136930684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.053.1279871
GTATCAA23450.045.7023351
ATCAACG25200.040.858423
CAACGCA25750.039.2923245
TCAACGC26700.038.1171884
GTACATG61300.038.0745121
AACGCAG27200.037.1903046
TATCAAC28650.036.1472322
TACATGG66650.034.201812
ACATGGG64850.033.8651053
ACGCAGA32300.031.126717
TAAGGTA6950.030.8285944
GTGTAAG7950.030.706161
CGCAGAG33650.029.8779418
CATGGGA37000.028.1496094
CATGGGG34500.026.9117264
TAAGGTG10400.026.8967975
GTATAGG4950.024.0565621
GGTAAGG10700.023.9177723
GAGTACA47100.023.5126081
Produced by FastQC (version 0.11.5)