##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364372_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1509815 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.063157406702146 31.0 31.0 34.0 28.0 34.0 2 31.05832900057292 33.0 31.0 34.0 27.0 34.0 3 31.203336832658305 33.0 31.0 34.0 27.0 34.0 4 34.62477919480201 37.0 35.0 37.0 32.0 37.0 5 34.689528849561036 37.0 35.0 37.0 32.0 37.0 6 34.73226984763034 37.0 35.0 37.0 32.0 37.0 7 34.68182989306637 37.0 35.0 37.0 32.0 37.0 8 34.572539019681216 37.0 35.0 37.0 31.0 37.0 9 36.11046982577336 39.0 37.0 39.0 31.0 39.0 10-11 36.03094617552482 39.0 37.0 39.0 31.0 39.0 12-13 36.030620638952456 39.0 37.0 39.0 31.0 39.0 14-15 37.34445610886102 40.0 38.0 41.0 31.0 41.0 16-17 37.35316412938009 40.0 38.0 41.0 31.0 41.0 18-19 37.35697022482887 40.0 38.0 41.0 31.0 41.0 20-21 37.39102903335839 40.0 38.0 41.0 31.0 41.0 22-23 37.369591969877106 40.0 38.0 41.0 31.0 41.0 24-25 37.3787824998427 40.0 38.0 41.0 31.0 41.0 26-27 37.340449988905924 40.0 38.0 41.0 31.0 41.0 28-29 37.35113441050724 40.0 38.0 41.0 31.0 41.0 30-31 37.25586810304574 40.0 37.5 41.0 30.0 41.0 32-33 37.209048459579485 40.0 37.0 41.0 30.0 41.0 34-35 37.19850544603147 40.0 37.0 41.0 30.0 41.0 36-37 37.132649033159694 40.0 37.0 41.0 30.0 41.0 38-39 37.03429923533678 40.0 37.0 41.0 30.0 41.0 40-41 36.96534939711157 40.0 37.0 41.0 30.0 41.0 42-43 36.82867735451032 40.0 37.0 41.0 30.0 41.0 44-45 36.69668866715459 40.0 36.0 41.0 30.0 41.0 46-47 36.57947066362435 40.0 36.0 41.0 30.0 41.0 48-49 36.41990641237503 40.0 36.0 41.0 29.5 41.0 50-51 36.28475607938721 40.0 35.5 41.0 29.0 41.0 52-53 36.11184979616708 39.0 35.0 41.0 29.0 41.0 54-55 35.91931925434573 39.0 35.0 41.0 29.0 41.0 56-57 35.713832489410954 39.0 35.0 41.0 28.0 41.0 58-59 35.47221845060487 38.5 35.0 41.0 28.0 41.0 60-61 35.20632031076655 38.0 34.0 40.0 28.0 41.0 62-63 34.9188327709024 37.5 34.0 40.0 28.0 41.0 64-65 34.556063160056034 37.0 34.0 40.0 27.0 41.0 66-67 34.233952172948335 36.5 34.0 39.5 26.0 41.0 68-69 33.906755132251305 36.0 33.5 39.0 26.0 41.0 70-71 33.544530621301284 35.5 33.0 39.0 26.0 40.5 72-73 33.19110023413465 35.0 33.0 38.0 26.0 40.0 74-75 32.84192500405679 35.0 33.0 37.0 25.5 39.5 76-77 32.62015611184152 35.0 33.0 37.0 25.5 39.0 78-79 32.464244625997225 35.0 33.0 36.5 26.0 39.0 80-81 32.21453787384547 35.0 33.0 36.0 25.5 38.0 82-83 31.938115265777597 35.0 33.0 36.0 25.0 37.0 84-85 31.705469213115514 35.0 33.0 35.0 25.0 37.0 86-87 31.511702095952153 35.0 32.5 35.0 24.0 36.0 88-89 31.252522660061 35.0 32.0 35.0 23.0 36.0 90-91 31.13497481479519 35.0 32.0 35.0 23.0 36.0 92-93 30.99202054556353 35.0 32.0 35.0 22.5 36.0 94-95 30.886677838013266 35.0 32.0 35.0 20.5 35.0 96-97 30.788498590886963 35.0 32.0 35.0 20.0 35.0 98-99 30.652363369022034 35.0 32.0 35.0 19.5 35.0 100-101 30.55967154916331 35.0 32.0 35.0 19.0 35.0 102-103 29.518378079433575 33.5 29.5 34.5 17.5 35.0 104-105 30.23408596417442 34.0 31.0 35.0 18.0 35.0 106-107 30.571709116679855 35.0 32.0 35.0 19.0 35.0 108-109 30.60693363094154 35.0 32.0 35.0 18.0 35.0 110-111 30.52225040816259 35.0 32.0 35.0 17.0 35.0 112-113 30.421871884966038 35.0 32.0 35.0 12.5 35.0 114-115 30.316397042021705 35.0 32.0 35.0 7.5 35.0 116-117 30.180729427115242 35.0 32.0 35.0 2.0 35.0 118-119 30.001064368813395 35.0 32.0 35.0 2.0 35.0 120-121 29.815222725963114 35.0 32.0 35.0 2.0 35.0 122-123 29.634120074313742 35.0 32.0 35.0 2.0 35.0 124-125 28.810924517242178 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29373249213285035 1101 2 0.2622752904903365 1101 3 0.23887190809821846 1101 4 0.42916570692300837 1101 5 0.191899991431832 1101 6 0.1390001687790985 1101 7 0.23667040383039506 1101 8 0.09545992085203636 1101 9 0.2801817454745219 1101 10-11 0.34315838200646454 1101 12-13 0.40874194395767205 1101 14-15 0.47532930125176875 1101 16-17 0.4445186248296551 1101 18-19 0.24699342323457074 1101 20-21 0.4324505914151331 1101 22-23 0.4258893992372137 1101 24-25 0.5001698526206368 1101 26-27 0.5258224373565525 1101 28-29 0.44656141995285026 1101 30-31 0.47632576205807453 1101 32-33 0.4243437473469811 1101 34-35 0.45694267924872634 1101 36-37 0.38882809577035005 1101 38-39 0.5313063906094584 1101 40-41 0.3851139115891442 1101 42-43 0.5146192080322862 1101 44-45 0.5624229277931434 1101 46-47 0.517702437873794 1101 48-49 0.38832702456440416 1101 50-51 0.40094221922076656 1101 52-53 0.29239589194870774 1101 54-55 0.23483322533680706 1101 56-57 0.10293332708596381 1101 58-59 0.2904640245270045 1101 60-61 0.2569478465307071 1101 62-63 0.2435474990078461 1101 64-65 0.31808239554928974 1101 66-67 0.3168130201549815 1101 68-69 0.24571937028596125 1101 70-71 0.22457216218432308 1101 72-73 0.35554048960985085 1101 74-75 0.3348359381341268 1101 76-77 0.38534058519832115 1101 78-79 0.4482236599374261 1101 80-81 0.41854151853048194 1101 82-83 0.4972964324088558 1101 84-85 0.42908023350151225 1101 86-87 0.3097168641963286 1101 88-89 0.33663208147380175 1101 90-91 0.19365468055131174 1101 92-93 0.18589255171231756 1101 94-95 0.1693751085645765 1101 96-97 0.18146350801338684 1101 98-99 0.04855540180989948 1101 100-101 0.10564024637904268 1101 102-103 -0.117254136744954 1101 104-105 0.024650138826469714 1101 106-107 0.03625122227279576 1101 108-109 0.1062386440793901 1101 110-111 0.08639258651330195 1101 112-113 0.1369925044376501 1101 114-115 0.010928470012657954 1101 116-117 0.002722769659783353 1101 118-119 0.09021817958416634 1101 120-121 -0.004341528870948963 1101 122-123 -0.03938743179779891 1101 124-125 -0.1915699344399151 1102 1 0.22808039175295036 1102 2 0.23010731742932222 1102 3 0.302334786118891 1102 4 0.34301370543895615 1102 5 0.2852697517435132 1102 6 0.25894363229594575 1102 7 0.30829863898383536 1102 8 0.3435672836581105 1102 9 0.3608101169936617 1102 10-11 0.45391917158436 1102 12-13 0.4128047614532733 1102 14-15 0.3371554222863651 1102 16-17 0.4304342590960317 1102 18-19 0.512301701861233 1102 20-21 0.3849109685789145 1102 22-23 0.4286625780282094 1102 24-25 0.5219490523873773 1102 26-27 0.5261685728221579 1102 28-29 0.413800071807934 1102 30-31 0.5102269718634673 1102 32-33 0.4803941461449952 1102 34-35 0.4093863690179802 1102 36-37 0.3136258916419479 1102 38-39 0.44136297213833586 1102 40-41 0.4441670176353938 1102 42-43 0.29031083399058843 1102 44-45 0.34616228010929717 1102 46-47 0.29728929074585153 1102 48-49 0.23692792474184898 1102 50-51 0.20191412174344947 1102 52-53 0.21103266081048133 1102 54-55 0.2660040497923788 1102 56-57 0.21429250820377632 1102 58-59 0.25470795451284545 1102 60-61 0.18042324316857616 1102 62-63 0.20651919432018673 1102 64-65 0.23659713144004257 1102 66-67 0.1196802216645878 1102 68-69 0.08059115171754883 1102 70-71 0.052617877864705065 1102 72-73 0.040501215878016694 1102 74-75 -0.02083120342371103 1102 76-77 0.07481857892713606 1102 78-79 0.20398173243893325 1102 80-81 0.17998917355227917 1102 82-83 0.15018828621662905 1102 84-85 0.051483091366268496 1102 86-87 0.016314691436132733 1102 88-89 0.1625550172431005 1102 90-91 0.11443296425176186 1102 92-93 0.14785235618109738 1102 94-95 0.01817953352924917 1102 96-97 -0.03696987938847229 1102 98-99 -0.09376391261050898 1102 100-101 -0.17652209596603186 1102 102-103 -0.3745633179241956 1102 104-105 -0.21142960521551757 1102 106-107 -0.24289233968944401 1102 108-109 -0.10435228215495584 1102 110-111 -0.006178943030757722 1102 112-113 -0.014653732626548788 1102 114-115 -0.09189014353717795 1102 116-117 -0.12472950951070771 1102 118-119 -0.07712229609052557 1102 120-121 -0.23695469704753336 1102 122-123 -0.29121516219338517 1102 124-125 -0.3608546451419201 1103 1 0.27537749072459405 1103 2 0.2400216169470717 1103 3 0.36810036678071256 1103 4 0.3175720937018838 1103 5 0.3235434300418518 1103 6 0.22245171168650302 1103 7 0.36257204121285724 1103 8 0.34150707674891834 1103 9 0.39345095002487795 1103 10-11 0.45072692183364893 1103 12-13 0.3222324094775928 1103 14-15 0.4868204899193813 1103 16-17 0.3525414612525424 1103 18-19 0.41918463340001466 1103 20-21 0.39096009939689225 1103 22-23 0.38523937408518094 1103 24-25 0.42940811995534744 1103 26-27 0.5241660786787179 1103 28-29 0.38919063452128455 1103 30-31 0.48872708252917363 1103 32-33 0.40631356405126695 1103 34-35 0.4192955120686861 1103 36-37 0.2810332517450149 1103 38-39 0.19915241058320987 1103 40-41 0.30434842214594937 1103 42-43 0.224301907749485 1103 44-45 0.2165850231435016 1103 46-47 0.15525361275372518 1103 48-49 0.32251000657419127 1103 50-51 0.2778417106667277 1103 52-53 0.24796387274682985 1103 54-55 0.22279860741907243 1103 56-57 0.15285915316039222 1103 58-59 0.09705214789463668 1103 60-61 0.07639866030692133 1103 62-63 0.11529712749712928 1103 64-65 0.15356650916553605 1103 66-67 0.20382087263285342 1103 68-69 0.13626453474864064 1103 70-71 0.08451796457268301 1103 72-73 0.1091379570543225 1103 74-75 0.11023746247133914 1103 76-77 0.13533428116258506 1103 78-79 0.22434085260286452 1103 80-81 0.1928404777021271 1103 82-83 0.08191368768725127 1103 84-85 0.01370443390402798 1103 86-87 -0.0130888719205835 1103 88-89 0.11626104943690763 1103 90-91 0.09677821272604348 1103 92-93 0.14009821970290304 1103 94-95 0.08060531015268069 1103 96-97 0.00454662170271547 1103 98-99 0.019210115454953325 1103 100-101 -0.004418067020008465 1103 102-103 -0.14474315169556107 1103 104-105 0.03541166206955637 1103 106-107 0.03982742695265529 1103 108-109 0.12460080430789233 1103 110-111 0.05461362181974749 1103 112-113 -0.009737239627050087 1103 114-115 -0.045179691854464465 1103 116-117 -0.03621458788503773 1103 118-119 -0.04277229256033266 1103 120-121 -0.3012820200557691 1103 122-123 -0.29355183506632443 1103 124-125 -0.28364785574076024 1104 1 0.12954889654806934 1104 2 0.15876592995889993 1104 3 0.21994010745405035 1104 4 0.22492536940707453 1104 5 0.1540512780855252 1104 6 0.21023037410688517 1104 7 0.14587220044852955 1104 8 0.2218386281592899 1104 9 0.3015662821449894 1104 10-11 0.35930005650153163 1104 12-13 0.23333483213455253 1104 14-15 0.2987674303197423 1104 16-17 0.276057157339892 1104 18-19 0.2573898199332234 1104 20-21 0.23368786327314695 1104 22-23 0.2791230183072031 1104 24-25 0.20994724643396268 1104 26-27 0.22008282117189282 1104 28-29 0.2183250749216512 1104 30-31 0.2509967595082472 1104 32-33 0.25800402128511735 1104 34-35 0.22886244791946808 1104 36-37 0.14899844737740864 1104 38-39 0.1480619306014006 1104 40-41 0.11879337210045549 1104 42-43 0.08720568390963024 1104 44-45 0.040099297483813245 1104 46-47 0.13197819191660187 1104 48-49 0.07142805707464106 1104 50-51 0.04816131940194168 1104 52-53 -0.06519297438693883 1104 54-55 0.04205901460742467 1104 56-57 -0.10041939552021972 1104 58-59 0.0619136697326752 1104 60-61 -0.12824903077587635 1104 62-63 -0.052494614996049904 1104 64-65 0.01161075393351041 1104 66-67 -0.07524248041536907 1104 68-69 -0.05741579964626453 1104 70-71 -0.07604988119619804 1104 72-73 -0.17623226587561902 1104 74-75 -0.21372181869335805 1104 76-77 -0.18908706779101436 1104 78-79 -0.03150252774290152 1104 80-81 -0.039549763426173 1104 82-83 -0.2142120516212067 1104 84-85 -0.0714894923926046 1104 86-87 -0.05570431778045659 1104 88-89 -0.011434582956177053 1104 90-91 -0.12130214441681275 1104 92-93 -0.27833046546543017 1104 94-95 -0.1373755246971804 1104 96-97 -0.14414679276497822 1104 98-99 -0.18962462066879482 1104 100-101 -0.2277759491856095 1104 102-103 -0.4573952786291393 1104 104-105 -0.3339738588585597 1104 106-107 -0.27499997295635126 1104 108-109 -0.271870419531961 1104 110-111 -0.2977777567061821 1104 112-113 -0.38361974645143704 1104 114-115 -0.43123093161788617 1104 116-117 -0.42627799780314746 1104 118-119 -0.4686537757268461 1104 120-121 -0.47982643543338455 1104 122-123 -0.5991734228734202 1104 124-125 -0.6947886655678737 1105 1 0.11760678611594244 1105 2 0.16764539619323315 1105 3 0.12493617124403755 1105 4 0.25788547728260625 1105 5 0.1342457112712836 1105 6 0.14065182138505605 1105 7 0.1546579381176656 1105 8 0.28455200191996965 1105 9 0.3208541009390302 1105 10-11 0.16759474222828885 1105 12-13 0.29162981015413436 1105 14-15 0.4984995926630731 1105 16-17 0.44828661110306456 1105 18-19 0.3220644785352107 1105 20-21 0.4252562870743546 1105 22-23 0.46598328674892997 1105 24-25 0.3926536441392656 1105 26-27 0.38328744173684015 1105 28-29 0.34258265115161635 1105 30-31 0.477643537272165 1105 32-33 0.5350123652709016 1105 34-35 0.3680804477012174 1105 36-37 0.27451716970701767 1105 38-39 0.290106986565732 1105 40-41 0.37314424819948044 1105 42-43 0.2071240757534909 1105 44-45 0.1624705132660651 1105 46-47 0.23624615428488482 1105 48-49 0.10946733733279501 1105 50-51 0.14864028990764666 1105 52-53 0.21166932061836263 1105 54-55 0.19531350032794847 1105 56-57 -9.93263762410379E-4 1105 58-59 0.07801754280743722 1105 60-61 0.03712274620859546 1105 62-63 0.06789241967378246 1105 64-65 0.03838263302560563 1105 66-67 -0.06754095425711171 1105 68-69 -0.1114801313479532 1105 70-71 -0.008168672896637474 1105 72-73 -0.11765347152505257 1105 74-75 -0.10837363948278522 1105 76-77 0.0030365175117239573 1105 78-79 0.1218976172343389 1105 80-81 0.011145698630322443 1105 82-83 0.0994521506921906 1105 84-85 0.0413478135249612 1105 86-87 0.09318984103977002 1105 88-89 0.04440581736807303 1105 90-91 -0.036460547640608354 1105 92-93 0.022769919581648423 1105 94-95 -0.09455491844289199 1105 96-97 -0.12305737844185316 1105 98-99 -0.15130132921777673 1105 100-101 -0.18234945498469912 1105 102-103 -0.32613816617044833 1105 104-105 -0.1583474753392018 1105 106-107 -0.08507481940518957 1105 108-109 -0.07245792810588725 1105 110-111 -0.1102257285837176 1105 112-113 -0.05598657784134531 1105 114-115 -0.18910929493397077 1105 116-117 -0.3134018169401429 1105 118-119 -0.24326833892445165 1105 120-121 -0.24663103183545587 1105 122-123 -0.3559813124412976 1105 124-125 -0.48920743154655 1106 1 0.21257372233204208 1106 2 0.2324593097498422 1106 3 0.23480291233209982 1106 4 0.3051300732398232 1106 5 0.19903167878239003 1106 6 0.21190720337517632 1106 7 0.1632611377307569 1106 8 0.19597843652166347 1106 9 0.34209328562931063 1106 10-11 0.3587447429126769 1106 12-13 0.2856595272565059 1106 14-15 0.4181979616786293 1106 16-17 0.3249900841691584 1106 18-19 0.289184245020337 1106 20-21 0.15336883017209857 1106 22-23 0.25720446430023003 1106 24-25 0.18708139277542557 1106 26-27 0.27583195009174943 1106 28-29 0.23977542335370572 1106 30-31 0.44835085358490545 1106 32-33 0.31993237494365445 1106 34-35 0.3350198956546535 1106 36-37 0.34893311636695046 1106 38-39 0.23797373373729158 1106 40-41 0.3133491212293791 1106 42-43 0.2758163110873255 1106 44-45 0.3576570065430431 1106 46-47 0.27256508216051145 1106 48-49 0.16230893686558545 1106 50-51 0.12679807898381767 1106 52-53 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-0.5969262937523538 2216 98-99 -0.7338402120526766 2216 100-101 -0.6202630711260575 2216 102-103 -0.7627190489612055 2216 104-105 -0.7421168361749864 2216 106-107 -0.6098466542170726 2216 108-109 -0.5032651150056431 2216 110-111 -0.4590843032425873 2216 112-113 -0.559813849501893 2216 114-115 -0.47818461282663804 2216 116-117 -0.6404374486424125 2216 118-119 -0.6621270517233135 2216 120-121 -0.4769620009637059 2216 122-123 -0.5440272333499578 2216 124-125 -0.4124993265874686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24646.0 3 4879.0 4 3013.0 5 2484.0 6 2769.0 7 3191.0 8 3477.0 9 4134.0 10 4482.0 11 5220.0 12 5750.0 13 5835.0 14 5523.0 15 5090.0 16 4817.0 17 4292.0 18 4106.0 19 4018.0 20 4214.0 21 4410.0 22 5022.0 23 6846.0 24 10087.0 25 14380.0 26 17219.0 27 17135.0 28 20181.0 29 24465.0 30 28734.0 31 36159.0 32 46227.0 33 60963.0 34 85573.0 35 130431.0 36 255631.0 37 459912.0 38 183979.0 39 521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86792035266296 18.063110131639018 12.346185370689032 24.722784145008987 2 15.885091336874025 19.856645686586482 38.74103621547792 25.517226761061572 3 18.675522744147628 24.12031477579742 29.49536122317726 27.708801256877692 4 12.500190430518693 15.950725192533536 36.864102820557484 34.68498155639029 5 13.130478678863296 38.83714213031173 34.59722766924944 13.435151521575527 6 33.18329290810543 36.30638192995625 16.994297624639668 13.51602753729865 7 29.19508985137481 32.62057623308435 20.621273027498873 17.563060888041964 8 26.992283150297418 35.10083699107108 19.59169777979127 18.315182078840227 9 26.864748330093423 15.79272957282846 18.936293519404696 38.406228577673424 10-11 24.929817537187514 26.635126368173047 27.692176754164173 20.74287934047527 12-13 25.578220749183338 23.90707668015186 27.466479092650438 23.048223478014364 14-15 22.46716809774992 25.62766092732075 24.548784881826347 27.35638609310298 16-17 22.882031456203034 28.845979983620495 25.651048954059558 22.620939606116913 18-19 23.091578468745663 27.735261517226125 26.66433525443026 22.50882475959795 20-21 23.464041443501834 26.999078363600297 26.68619224414655 22.850687948751318 22-23 23.48358026764215 26.832104208828305 26.58829942353845 23.096016099991093 24-25 23.181813665912713 27.127495752790903 26.610776817027254 23.079913764269133 26-27 22.80471448488722 27.365637511880596 26.355878038037773 23.47376996519441 28-29 23.09362665049687 27.6267120940012 26.290087398385424 22.989573857116504 30-31 22.873156187833796 27.118084452152537 26.36395157415138 23.644807785862294 32-33 22.727111659817588 27.309796872262698 26.247281535103507 23.715809932816207 34-35 23.534774758544497 27.366999116440432 25.9438986039229 23.15432752109217 36-37 23.604165811152896 26.914242416615558 25.994228428137507 23.48736334409404 38-39 23.040792786658752 27.009461764648414 26.2726820233095 23.677063425383334 40-41 23.33669358177094 26.778883951546188 26.390126168276918 23.494296298405956 42-43 22.930816539492366 26.889872165568455 26.27171266213606 23.907598632803122 44-45 23.001393235044084 27.014453281917984 26.64907267242484 23.335080810613093 46-47 23.638632428023442 27.393597027979265 25.854898068603795 23.1128724753935 48-49 23.144160046098364 27.245622808092385 26.007325400794134 23.602891745015118 50-51 23.177177336296168 27.330566989995464 26.26659557627921 23.225660097429156 52-53 23.456184204774413 26.911282171522284 26.245199688965016 23.38733393473829 54-55 22.765499577087063 27.2453630262153 26.42573324228176 23.563404154415878 56-57 22.95165030371311 27.450816756777165 26.28126794510314 23.31626499440659 58-59 23.08479212616157 26.826852385399487 26.214060047290715 23.874295441148224 60-61 22.524288756781928 27.06683353164832 26.703110702816623 23.705767008753124 62-63 22.8589075097322 27.120245461755886 26.784705997287734 23.236141031224182 64-65 22.71123652920977 27.137865451225316 26.30828235233338 23.842615667231534 66-67 22.5041653405909 27.31589128353426 26.381218991767348 23.79872438410749 68-69 22.98031163583978 26.67844546372804 26.833534798231522 23.50770810220066 70-71 22.733890143662364 26.7108008398519 26.496201458480982 24.059107558004754 72-73 22.714108535860415 26.944466982717653 26.30086221314068 24.040562268281253 74-75 22.709490296494973 27.307018627431173 26.293036214651117 23.690454861422737 76-77 23.025790493512222 26.914648657223257 26.444818371698776 23.614742477565745 78-79 23.023486865636052 26.90918544423691 26.529626833048525 23.537700857078512 80-81 23.103747813038876 26.914814242014096 26.469921025989855 23.511516918957174 82-83 22.805192052629224 27.05460795498764 26.553418233781457 23.586781758601678 84-85 22.677666748134033 27.17221472678296 26.613666514991653 23.536452010091356 86-87 22.97522002268531 27.071425614531847 26.397721533659542 23.555632829123304 88-89 22.762730579156155 27.231871108164302 26.286967678347995 23.718430634331547 90-91 22.6806428228197 27.199226127534633 26.430453643323876 23.689677406321792 92-93 22.876154873800917 26.65669623134766 26.765783744437165 23.70136515041426 94-95 22.539136793238576 26.577120619578547 26.996480660855525 23.88726192632735 96-97 22.8723992605697 26.33435575711132 26.87634412519183 23.91690085712715 98-99 22.58870430661433 26.643498119459757 26.513083970087536 24.254713603838375 100-101 22.495192216938563 26.41182573885202 26.912722717010922 24.180259327198492 102-103 22.353214683946234 26.084507303458043 27.119546085123282 24.44273192747244 104-105 22.217905997256604 26.529218635229533 26.753419476794054 24.49945589071981 106-107 22.45718969671378 26.481929506445763 26.48156519867193 24.579315598168524 108-109 22.396238156063273 26.404275027379608 26.483160091972962 24.716326724584157 110-111 22.458987022423223 26.545332433874368 26.76125557318486 24.234424970517548 112-113 22.426357112862352 26.78651146586337 26.858011665229377 23.929119756044898 114-115 22.2435094547326 27.045820795576553 26.525808846760405 24.184860902930442 116-117 22.099493804612223 26.90462431095664 26.830077643119832 24.165804241311307 118-119 21.973042021328233 27.19328035124955 26.432078424174517 24.401599203247706 120-121 21.964240646985687 27.307178433103896 26.622879388360328 24.105701531550093 122-123 21.887223610414257 27.33286637092381 26.613181255930172 24.16672876273176 124-125 21.999342288933864 27.39889844987503 26.504365035602834 24.09739422558827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 760.0 1 633.5 2 462.0 3 358.5 4 265.5 5 221.5 6 202.5 7 193.5 8 203.5 9 215.5 10 187.5 11 160.5 12 169.0 13 191.0 14 211.0 15 223.0 16 258.5 17 298.5 18 360.0 19 482.5 20 664.0 21 961.0 22 1404.0 23 1965.0 24 2783.0 25 4194.5 26 5935.5 27 7964.0 28 10154.0 29 12576.0 30 15251.0 31 18915.0 32 24778.5 33 27025.5 34 26270.0 35 28006.5 36 31329.0 37 34788.5 38 36992.0 39 38913.0 40 41235.5 41 44371.5 42 46359.5 43 47805.0 44 50324.5 45 53974.0 46 55309.0 47 55205.5 48 55134.5 49 54184.5 50 54769.5 51 55127.5 52 55909.0 53 58626.0 54 61422.0 55 58771.5 56 52901.5 57 48842.0 58 46514.0 59 40781.5 60 31396.5 61 25304.0 62 21466.0 63 16356.0 64 11601.0 65 8408.0 66 5824.5 67 4456.5 68 3368.0 69 2254.5 70 1598.5 71 1133.0 72 797.0 73 566.0 74 422.0 75 272.0 76 182.5 77 101.0 78 48.5 79 32.0 80 26.0 81 18.5 82 12.0 83 7.0 84 4.5 85 6.5 86 7.0 87 3.0 88 3.0 89 4.5 90 2.5 91 3.5 92 3.5 93 1.0 94 2.5 95 2.5 96 0.5 97 0.0 98 0.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04053476750462805 2 0.008544093150485325 3 0.0027817977699254545 4 0.005166195858432986 5 0.021658282637276753 6 0.02940757642492623 7 0.002914264330398095 8 1.3246656047264069E-4 9 0.0 10-11 3.311664011816017E-5 12-13 7.285660825995237E-4 14-15 1.9869984070896101E-4 16-17 9.934992035448051E-5 18-19 3.311664011816017E-5 20-21 3.311664011816017E-5 22-23 3.311664011816017E-5 24-25 0.0 26-27 0.0 28-29 2.9804976106344153E-4 30-31 9.934992035448051E-5 32-33 9.934992035448051E-5 34-35 8.610326430721645E-4 36-37 6.623328023632034E-5 38-39 9.934992035448051E-5 40-41 3.9739968141792203E-4 42-43 6.954494424813636E-4 44-45 0.0012915489646082466 46-47 3.311664011816017E-5 48-49 0.0 50-51 0.0 52-53 0.0011259657640174457 54-55 0.0031791974513433762 56-57 1.3246656047264069E-4 58-59 3.311664011816017E-4 60-61 7.61682722717684E-4 62-63 4.967496017724025E-4 64-65 0.001357782244844567 66-67 0.002285048168153052 68-69 0.0026493312094528136 70-71 0.0016558320059080086 72-73 0.002483748008862013 74-75 0.0016889486460261688 76-77 9.603825634266449E-4 78-79 0.002318164808271212 80-81 9.603825634266449E-4 82-83 4.967496017724025E-4 84-85 5.298662418905627E-4 86-87 0.0018214152064988096 88-89 0.005563595539850909 90-91 3.6428304129976186E-4 92-93 0.0011921990442537661 94-95 9.603825634266449E-4 96-97 2.6493312094528137E-4 98-99 6.292161622450433E-4 100-101 0.001556482085553528 102-103 0.0010928491238992857 104-105 5.298662418905627E-4 106-107 0.0067889112242228355 108-109 0.001556482085553528 110-111 0.0012253156843719264 112-113 0.0019207651268532899 114-115 0.0027817977699254545 116-117 0.0014902488053172077 118-119 0.04560161344270656 120-121 0.011127191079701818 122-123 0.0038084136135884197 124-125 0.0022188148879167314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1509815.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.66098423047602 #Duplication Level Percentage of deduplicated Percentage of total 1 84.5661447286338 63.13791598016682 2 9.772495931423572 14.59248329256813 3 2.827586943193884 6.333312725282955 4 1.1401588599051964 3.405015306384776 5 0.5784275850986574 2.1592986404761603 6 0.3014193495446387 1.3502559181867566 7 0.1874475956784482 0.9796515389492487 8 0.1270524980856501 0.7588691644812247 9 0.09337575250717714 0.6274373026902467 >10 0.38376223078619287 4.935870804108774 >50 0.016517140692311312 0.8218968062494414 >100 0.005290733955532369 0.5624722775844231 >500 0.0 0.0 >1k 3.2065049494089725E-4 0.33552024287106746 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1732 0.11471604136930684 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.623328023632034E-5 2 0.0 0.0 0.0 0.0 6.623328023632034E-5 3 0.0 0.0 0.0 0.0 6.623328023632034E-5 4 0.0 0.0 0.0 0.0 6.623328023632034E-5 5 0.0 0.0 0.0 0.0 6.623328023632034E-5 6 0.0 0.0 0.0 0.0 6.623328023632034E-5 7 0.0 0.0 0.0 0.0 6.623328023632034E-5 8 0.0 0.0 0.0 0.0 1.3246656047264069E-4 9 0.0 0.0 0.0 0.0 1.3246656047264069E-4 10-11 0.0 0.0 0.0 0.0 1.3246656047264069E-4 12-13 0.0 0.0 0.0 0.0 3.973996814179221E-4 14-15 0.0 0.0 0.0 0.0 5.629828820087229E-4 16-17 0.0 0.0 0.0 0.0 5.960995221268831E-4 18-19 3.311664011816017E-5 0.0 0.0 0.0 6.623328023632034E-4 20-21 6.623328023632034E-5 0.0 0.0 9.934992035448052E-5 8.279160029540044E-4 22-23 6.623328023632034E-5 0.0 0.0 2.9804976106344153E-4 9.60382563426645E-4 24-25 1.9869984070896104E-4 0.0 0.0 5.629828820087229E-4 0.0010597324837811255 26-27 1.9869984070896104E-4 0.0 0.0 0.0012584323244900866 0.001125965764017446 28-29 2.318164808271212E-4 0.0 0.0 0.0038084136135884197 0.001125965764017446 30-31 2.6493312094528137E-4 0.0 0.0 0.012849256365846147 0.0011590824041356061 32-33 2.6493312094528137E-4 0.0 0.0 0.03281859035709673 0.0011921990442537661 34-35 3.6428304129976186E-4 0.0 0.0 0.06017293509469703 0.0011921990442537661 36-37 4.636329616542424E-4 0.0 0.0 0.10650311462000311 0.0011921990442537661 38-39 4.636329616542424E-4 0.0 0.0 0.18386358593602528 0.0011921990442537661 40-41 4.636329616542424E-4 0.0 0.0 0.2633435222196097 0.0011921990442537661 42-43 4.967496017724026E-4 0.0 0.0 0.33961114441173257 0.0012253156843719264 44-45 5.960995221268831E-4 0.0 0.0 0.43568251739451525 0.0012584323244900866 46-47 5.960995221268831E-4 0.0 0.0 0.5571212367078086 0.0012584323244900866 48-49 5.960995221268831E-4 0.0 0.0 0.6892566307792676 0.0014571321651990477 50-51 5.960995221268831E-4 0.0 0.0 0.8355659468212993 0.0014571321651990477 52-53 5.960995221268831E-4 0.0 0.0 0.963462410957634 0.0016227153657898483 54-55 5.960995221268831E-4 0.0 0.0 1.1029496991353245 0.001722065286144329 56-57 5.960995221268831E-4 0.0 0.0 1.2720432635786503 0.001722065286144329 58-59 5.960995221268831E-4 0.0 0.0 1.4559399661547938 0.001722065286144329 60-61 6.292161622450433E-4 0.0 0.0 1.6690455453151545 0.001722065286144329 62-63 6.623328023632034E-4 0.0 0.0 1.8733089815639665 0.0017882985663806492 64-65 6.623328023632034E-4 0.0 0.0 2.0784003338157326 0.0018545318466169696 66-67 7.947993628358442E-4 0.0 0.0 2.317370008908376 0.0018545318466169696 68-69 8.610326430721645E-4 0.0 0.0 2.5948543364584404 0.0018545318466169696 70-71 9.934992035448053E-4 0.0 0.0 2.8915794319171555 0.00192076512685329 72-73 0.0010597324837811255 0.0 0.0 3.195590188201866 0.0023512814483893723 74-75 0.0010597324837811255 0.0 0.0 3.4947327983892063 0.0024506313687438527 76-77 0.0010597324837811255 0.0 0.0 3.8223557190781654 0.002516864648980173 78-79 0.0010597324837811255 0.0 0.0 4.187168626619817 0.0025830979292164936 80-81 0.0013246656047264068 0.0 0.0 4.586985822766366 0.0025830979292164936 82-83 0.0013246656047264068 0.0 0.0 5.005646387140146 0.0026493312094528136 84-85 0.001357782244844567 0.0 0.0 5.415067408920961 0.002715564489689134 86-87 0.0013908988849627272 0.0 0.0 5.82962150992009 0.002914264330398095 88-89 0.0013908988849627272 0.0 0.0 6.274510453267453 0.0031129641711070562 90-91 0.0014571321651990477 0.0 0.0 6.786493709494209 0.0031129641711070562 92-93 0.0014571321651990477 0.0 0.0 7.29191987097757 0.0031791974513433767 94-95 0.0014571321651990477 0.0 0.0 7.794067485089233 0.0031791974513433767 96-97 0.0014571321651990477 0.0 3.311664011816017E-5 8.318568831280654 0.003411013932170498 98-99 0.0014902488053172079 0.0 6.623328023632034E-5 8.85340256918894 0.0035103638525249784 100-101 0.0016889486460261688 0.0 6.623328023632034E-5 9.452913105247994 0.0035103638525249784 102-103 0.0019207651268532899 0.0 6.623328023632034E-5 10.079777986044649 0.003609713772879459 104-105 0.0021856982477985714 0.0 6.623328023632034E-5 10.706113000599412 0.003642830412997619 106-107 0.0021856982477985714 0.0 6.623328023632034E-5 11.310624149316308 0.003642830412997619 108-109 0.0021856982477985714 0.0 6.623328023632034E-5 11.945105857340138 0.003642830412997619 110-111 0.0021856982477985714 0.0 6.623328023632034E-5 12.622009981355331 0.0037090636932339393 112-113 0.0021856982477985714 0.0 6.623328023632034E-5 13.33213009540904 0.0037752969734702597 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1625 0.0 53.127987 1 GTATCAA 2345 0.0 45.702335 1 ATCAACG 2520 0.0 40.85842 3 CAACGCA 2575 0.0 39.292324 5 TCAACGC 2670 0.0 38.117188 4 GTACATG 6130 0.0 38.074512 1 AACGCAG 2720 0.0 37.190304 6 TATCAAC 2865 0.0 36.147232 2 TACATGG 6665 0.0 34.20181 2 ACATGGG 6485 0.0 33.865105 3 ACGCAGA 3230 0.0 31.12671 7 TAAGGTA 695 0.0 30.828594 4 GTGTAAG 795 0.0 30.70616 1 CGCAGAG 3365 0.0 29.877941 8 CATGGGA 3700 0.0 28.149609 4 CATGGGG 3450 0.0 26.911726 4 TAAGGTG 1040 0.0 26.896797 5 GTATAGG 495 0.0 24.056562 1 GGTAAGG 1070 0.0 23.917772 3 GAGTACA 4710 0.0 23.512608 1 >>END_MODULE