FastQCFastQC Report
Thu 26 May 2016
SRR1364372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509815
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.22214642191261844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.22002695694505617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.11710043945781436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.073.505011
GTATCAA44700.051.7191051
ATCAACG56350.040.2716563
TCAACGC57850.039.2274444
CAACGCA57950.039.1597565
TATCAAC59000.038.5637932
AACGCAG60400.037.5352336
GTGGTAT8500.037.1521341
GTACATG60150.035.3638421
GTAAGGT8700.034.9155854
TACATGG62850.033.7375142
ACATGGG62600.033.3965153
TAAGGTG10200.032.7006385
ACGCAGA69800.032.476047
CGCAGAG70250.032.0139278
AGGTAAG9300.031.3820652
CATGGGA33200.029.2427314
TGGTATC11500.028.4861162
GGTAAGG10950.027.1972053
GCAGAGT82800.027.0178669
Produced by FastQC (version 0.11.5)