FastQCFastQC Report
Thu 26 May 2016
SRR1364371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477268
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.053.8463861
GTATCAA24800.042.010151
GTACATG67450.039.630751
TACATGG69500.037.7612422
AGGTAAG17750.037.550192
TCAACGC27950.035.7708054
TAAGGTG21550.035.3479275
ATCAACG28150.035.304543
CAACGCA28550.035.22755
ACATGGG71800.035.2254833
AACGCAG31100.031.9537836
CATGGGG48800.030.4874764
TAAGGTA12500.030.4699134
TATCAAC32950.030.342152
GTAAGGT23600.030.260114
GGTAAGG23350.030.073753
ACGCAGA36050.027.5617797
AAGGTAA23900.027.1516651
CGCAGAG37950.026.0245748
GTGTAAG15950.024.6349181
Produced by FastQC (version 0.11.5)