FastQCFastQC Report
Thu 26 May 2016
SRR1364371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477268
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28880.116580038978423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.11157452483946025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.074.8516161
GTATCAA53200.053.5058561
ATCAACG67400.041.3403853
TCAACGC68650.040.587654
CAACGCA69550.039.9768265
TATCAAC70900.039.2996062
AACGCAG72400.038.4651876
GTACATG71700.033.8033941
ACGCAGA82600.033.6384547
CGCAGAG84100.033.1092268
TACATGG72850.032.9354862
AGGTAAG17250.032.443472
ACATGGG72600.032.2288253
TAAGGTG21550.030.6665045
GTGTAAG15550.027.57321
GTAAGGT22750.027.4787184
CATGGGG51750.027.2663214
TAAGGTA12600.026.4609894
GGTAAGG22750.026.431913
TGGTATC13300.026.4112512
Produced by FastQC (version 0.11.5)