FastQCFastQC Report
Thu 26 May 2016
SRR1364370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306571
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.044.2415851
ATCAACG26200.041.7974433
CAACGCA26200.041.3422285
GGTATCA16750.040.5213051
TCAACGC28200.038.622044
AACGCAG28650.038.2181746
GTACATG63250.037.558281
TACATGG64550.035.313092
TATCAAC32550.034.740482
ACATGGG68800.032.6126563
ACGCAGA34500.031.9039617
CGCAGAG34350.031.5222918
CATGGGG46950.026.240254
CATGGGA29200.023.6433144
TAAGGTG15200.022.7095345
TATTCCG4101.8189894E-1221.773655
TAAGGTA8750.021.0856424
GCAGAGT50400.020.8937249
GTAAGGT13550.020.204674
AGGTAAG11500.020.1836642
Produced by FastQC (version 0.11.5)