##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2306571 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.134024489165952 31.0 31.0 34.0 28.0 34.0 2 31.11620106209607 31.0 31.0 34.0 28.0 34.0 3 31.274227413767015 33.0 31.0 34.0 28.0 34.0 4 34.7507776695363 37.0 35.0 37.0 32.0 37.0 5 34.79212302591162 37.0 35.0 37.0 32.0 37.0 6 34.86882909739175 37.0 35.0 37.0 32.0 37.0 7 34.76979334258516 37.0 35.0 37.0 32.0 37.0 8 34.676004770718095 37.0 35.0 37.0 32.0 37.0 9 36.25124741445202 39.0 37.0 39.0 32.0 39.0 10-11 36.17412015498331 39.0 37.0 39.0 31.5 39.0 12-13 36.22178961757518 39.0 37.0 39.0 32.0 39.0 14-15 37.532403294760925 40.0 38.0 41.0 32.0 41.0 16-17 37.525610310716644 40.0 38.0 41.0 32.0 41.0 18-19 37.51495054780452 40.0 38.0 41.0 32.0 41.0 20-21 37.54365918066255 40.0 38.0 41.0 32.0 41.0 22-23 37.493125943229145 40.0 38.0 41.0 31.5 41.0 24-25 37.486055274257765 40.0 38.0 41.0 31.0 41.0 26-27 37.42963992870803 40.0 38.0 41.0 31.0 41.0 28-29 37.4264410243604 40.0 38.0 41.0 31.0 41.0 30-31 37.32405289063289 40.0 37.5 41.0 30.5 41.0 32-33 37.28371682467177 40.0 37.0 41.0 30.5 41.0 34-35 37.27275813317691 40.0 37.0 41.0 30.0 41.0 36-37 37.20368503722625 40.0 37.0 41.0 30.0 41.0 38-39 37.099787736861344 40.0 37.0 41.0 30.0 41.0 40-41 37.03902871405216 40.0 37.0 41.0 30.0 41.0 42-43 36.89417299532509 40.0 37.0 41.0 30.0 41.0 44-45 36.74828804316017 40.0 36.0 41.0 30.0 41.0 46-47 36.62851956432297 40.0 36.0 41.0 30.0 41.0 48-49 36.46748853601298 40.0 36.0 41.0 30.0 41.0 50-51 36.332219992360955 40.0 35.5 41.0 29.5 41.0 52-53 36.16082336073765 39.0 35.0 41.0 29.0 41.0 54-55 35.97655914342113 39.0 35.0 41.0 29.0 41.0 56-57 35.76930170369783 39.0 35.0 41.0 28.0 41.0 58-59 35.533780013708665 38.5 35.0 40.5 28.0 41.0 60-61 35.274531761649655 38.0 34.5 40.0 28.0 41.0 62-63 34.997806050626664 38.0 34.0 40.0 28.0 41.0 64-65 34.64998215966472 37.0 34.0 40.0 27.0 41.0 66-67 34.33042165188065 37.0 34.0 39.5 26.0 41.0 68-69 34.003354329868884 36.0 33.5 39.0 26.0 41.0 70-71 33.63794632812083 36.0 33.0 39.0 26.0 40.5 72-73 33.280585986730955 35.0 33.0 38.5 26.0 40.0 74-75 32.92501965038146 35.0 33.0 37.0 26.0 39.5 76-77 32.70356472876837 35.0 33.0 37.0 25.5 39.0 78-79 32.552207584331896 35.0 33.0 36.5 26.0 39.0 80-81 32.29503232287235 35.0 33.0 36.0 26.0 38.0 82-83 32.00152238972917 35.0 33.0 36.0 25.0 37.0 84-85 31.75983483708067 35.0 33.0 35.0 25.0 37.0 86-87 31.558251621129372 35.0 32.0 35.0 24.5 36.5 88-89 31.29736435600725 35.0 32.0 35.0 24.0 36.0 90-91 31.17001861204359 35.0 32.0 35.0 23.0 36.0 92-93 31.020864304632287 35.0 32.0 35.0 23.0 36.0 94-95 30.912849853743936 35.0 32.0 35.0 22.0 35.0 96-97 30.802019968169198 35.0 32.0 35.0 20.0 35.0 98-99 30.663418338303916 35.0 32.0 35.0 20.0 35.0 100-101 30.55720374529984 35.0 32.0 35.0 19.0 35.0 102-103 29.495006657068004 33.5 29.5 34.5 17.5 35.0 104-105 30.222532711978083 34.0 31.0 35.0 18.0 35.0 106-107 30.569663799640246 35.0 32.0 35.0 19.0 35.0 108-109 30.606244724311544 35.0 32.0 35.0 18.5 35.0 110-111 30.518285151421743 35.0 32.0 35.0 17.5 35.0 112-113 30.40487242751253 35.0 32.0 35.0 12.5 35.0 114-115 30.286929602427154 35.0 32.0 35.0 7.5 35.0 116-117 30.13853551440645 35.0 32.0 35.0 2.0 35.0 118-119 29.947478313045643 35.0 32.0 35.0 2.0 35.0 120-121 29.748879613937746 35.0 31.5 35.0 2.0 35.0 122-123 29.542634932980604 35.0 31.0 35.0 2.0 35.0 124-125 28.685031373411007 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30038078611484664 1101 2 0.2914268894158276 1101 3 0.3937831704368975 1101 4 0.44909479312711653 1101 5 0.35151900884748244 1101 6 0.30002647303474816 1101 7 0.3631551059360092 1101 8 0.23455038329202438 1101 9 0.43742509442451905 1101 10-11 0.5994134963745239 1101 12-13 0.6083778242419342 1101 14-15 0.7036439160573451 1101 16-17 0.5935330964139283 1101 18-19 0.5802564519632227 1101 20-21 0.6783226107200377 1101 22-23 0.6976640252431991 1101 24-25 0.726947906071338 1101 26-27 0.7803076037859569 1101 28-29 0.7358119353080852 1101 30-31 0.8140185981011001 1101 32-33 0.7059191173525221 1101 34-35 0.6793291068907692 1101 36-37 0.6028917808023166 1101 38-39 0.7112989403565493 1101 40-41 0.6545460394161964 1101 42-43 0.7790456186728889 1101 44-45 0.6942011310790335 1101 46-47 0.6719018557994545 1101 48-49 0.5814106873886544 1101 50-51 0.5303799719974478 1101 52-53 0.5469954569603104 1101 54-55 0.6348853272968711 1101 56-57 0.543897932376872 1101 58-59 0.5788394022282901 1101 60-61 0.4705527131570264 1101 62-63 0.5227809807519037 1101 64-65 0.5432879573159681 1101 66-67 0.5059856500305386 1101 68-69 0.5238681567440153 1101 70-71 0.3737468878454706 1101 72-73 0.43398717178270374 1101 74-75 0.3992949051665491 1101 76-77 0.44391009176066376 1101 78-79 0.4159971704821501 1101 80-81 0.455470307260164 1101 82-83 0.4406387364157638 1101 84-85 0.4340314325531125 1101 86-87 0.3958649233789302 1101 88-89 0.38457423621702347 1101 90-91 0.29428894452206933 1101 92-93 0.3429357607349566 1101 94-95 0.28370771011305607 1101 96-97 0.2420126303074106 1101 98-99 0.24002874204134628 1101 100-101 0.19754810355756192 1101 102-103 -0.05192812414969339 1101 104-105 0.08361953216217444 1101 106-107 0.13739635067995692 1101 108-109 0.20147034124761376 1101 110-111 0.10848264360944526 1101 112-113 0.13372513711860634 1101 114-115 0.04151232063806276 1101 116-117 0.03216768307802553 1101 118-119 0.19308239493369328 1101 120-121 0.14001845009899938 1101 122-123 0.03574687568340451 1101 124-125 -0.028714075813009998 1102 1 0.40285562520990936 1102 2 0.44533336829274717 1102 3 0.5329648177672262 1102 4 0.5172718077336214 1102 5 0.5199618113964988 1102 6 0.5400826826555019 1102 7 0.5478781340089967 1102 8 0.5280245018137038 1102 9 0.6664303359226977 1102 10-11 0.6226819781027331 1102 12-13 0.6414156653616203 1102 14-15 0.6946077788529905 1102 16-17 0.6890878195970558 1102 18-19 0.7453718049095386 1102 20-21 0.7374275818603806 1102 22-23 0.7192685781571981 1102 24-25 0.7515220700470806 1102 26-27 0.6673669054254887 1102 28-29 0.6582662425498071 1102 30-31 0.6941557437001791 1102 32-33 0.7660417045945351 1102 34-35 0.6773542624913702 1102 36-37 0.5044229380529757 1102 38-39 0.5880943554533289 1102 40-41 0.6046420478639689 1102 42-43 0.5137530193614737 1102 44-45 0.4460741730656963 1102 46-47 0.44142608966999575 1102 48-49 0.4286666737377445 1102 50-51 0.408176682167813 1102 52-53 0.49776838004827795 1102 54-55 0.4465622884520073 1102 56-57 0.44699672437597826 1102 58-59 0.4061678213000661 1102 60-61 0.48418158766708785 1102 62-63 0.4236825543438272 1102 64-65 0.4193464779942957 1102 66-67 0.4280996638364911 1102 68-69 0.4177117017366001 1102 70-71 0.3446987220434039 1102 72-73 0.47783198165497254 1102 74-75 0.34934195053003236 1102 76-77 0.4083783975481836 1102 78-79 0.5048863857916075 1102 80-81 0.4258668184772034 1102 82-83 0.4225827830351463 1102 84-85 0.42303718000314916 1102 86-87 0.3858136182271146 1102 88-89 0.3869024308746596 1102 90-91 0.32734269864414856 1102 92-93 0.3187345322511739 1102 94-95 0.33121822289527714 1102 96-97 0.2655797868497345 1102 98-99 0.28922113676867767 1102 100-101 0.20665197302851013 1102 102-103 -0.047086083139845414 1102 104-105 0.14838667597409483 1102 106-107 0.07171033298632778 1102 108-109 0.20594679122815407 1102 110-111 0.25753402465404207 1102 112-113 0.2854645914903138 1102 114-115 0.10694617844763243 1102 116-117 0.07161234377609205 1102 118-119 0.026381862133728617 1102 120-121 -0.0902092364034246 1102 122-123 -0.09325820260449191 1102 124-125 -0.12530028797168669 1103 1 0.33199851330426 1103 2 0.3656427706704193 1103 3 0.3710449845957733 1103 4 0.429115625093786 1103 5 0.32305896549466695 1103 6 0.2969551890599149 1103 7 0.2501469311935338 1103 8 0.3206707788327279 1103 9 0.5734986544846024 1103 10-11 0.573307004811582 1103 12-13 0.5262292890129245 1103 14-15 0.5814357115904727 1103 16-17 0.4754644408793425 1103 18-19 0.49952862557218936 1103 20-21 0.5672702550704756 1103 22-23 0.5824072318762887 1103 24-25 0.5890537711464034 1103 26-27 0.6002721585093411 1103 28-29 0.5507318314362593 1103 30-31 0.7104156971990747 1103 32-33 0.7625200490132542 1103 34-35 0.7333580871110712 1103 36-37 0.6044404449911198 1103 38-39 0.5416012563172856 1103 40-41 0.5574149059371294 1103 42-43 0.5832177097753259 1103 44-45 0.45295138374529387 1103 46-47 0.5516521815322619 1103 48-49 0.47903609563244487 1103 50-51 0.44590250716139224 1103 52-53 0.5133739996435267 1103 54-55 0.5050165128647564 1103 56-57 0.5007227125683329 1103 58-59 0.4132436227370917 1103 60-61 0.3931419481253613 1103 62-63 0.3686482940308764 1103 64-65 0.49866016205376695 1103 66-67 0.35524011344561757 1103 68-69 0.22803800785003148 1103 70-71 0.31184503133287933 1103 72-73 0.40279202169494965 1103 74-75 0.248607635155075 1103 76-77 0.23115683216587968 1103 78-79 0.2783847239954014 1103 80-81 0.21670340039833036 1103 82-83 0.3354995268607155 1103 84-85 0.2990848484676434 1103 86-87 0.16072583293480847 1103 88-89 0.3306473891666464 1103 90-91 0.20135855987734885 1103 92-93 0.20888544188213487 1103 94-95 0.21821603712195525 1103 96-97 0.1794241082268222 1103 98-99 0.1840417649538466 1103 100-101 0.1800868870510648 1103 102-103 -0.046563732732717966 1103 104-105 0.1446394789747032 1103 106-107 0.13082267230895184 1103 108-109 0.23448419953655275 1103 110-111 0.2546120542468344 1103 112-113 0.17621778024148682 1103 114-115 0.12170019233746743 1103 116-117 0.10791382855085985 1103 118-119 0.136693774044371 1103 120-121 0.0700992286364226 1103 122-123 0.06692927023601314 1103 124-125 -0.035936953145291994 1104 1 0.4432094032270726 1104 2 0.41006998367581815 1104 3 0.495464462105609 1104 4 0.5361009878473268 1104 5 0.4018357126831731 1104 6 0.46067418879013644 1104 7 0.44107751198816914 1104 8 0.48604726363838324 1104 9 0.632437128129915 1104 10-11 0.600127020923523 1104 12-13 0.46460505368158067 1104 14-15 0.686097585482905 1104 16-17 0.6869584767308723 1104 18-19 0.6290358192269778 1104 20-21 0.648597579292705 1104 22-23 0.7117495672329142 1104 24-25 0.8014377620279376 1104 26-27 0.8189908369163703 1104 28-29 0.7391166253714374 1104 30-31 0.708205307319588 1104 32-33 0.7852970732688291 1104 34-35 0.6961494547592366 1104 36-37 0.6247258155954611 1104 38-39 0.5639410182057745 1104 40-41 0.6037494561160273 1104 42-43 0.5410220821107288 1104 44-45 0.49673790034584187 1104 46-47 0.4752886647881027 1104 48-49 0.5491653489468646 1104 50-51 0.4539538998812418 1104 52-53 0.4299078838101664 1104 54-55 0.5011404988526849 1104 56-57 0.4853223447168631 1104 58-59 0.4420435492820971 1104 60-61 0.24829110567360146 1104 62-63 0.3823893539972403 1104 64-65 0.45084646541101137 1104 66-67 0.4930407943794819 1104 68-69 0.4591280107309146 1104 70-71 0.31443181021454336 1104 72-73 0.2594889142198866 1104 74-75 0.2042579551471917 1104 76-77 0.32867240192786085 1104 78-79 0.46842581606017575 1104 80-81 0.46469987822134584 1104 82-83 0.370354360841759 1104 84-85 0.29281179734368123 1104 86-87 0.30256263839557107 1104 88-89 0.4033105174912599 1104 90-91 0.3107056481042214 1104 92-93 0.3504014752312443 1104 94-95 0.3390869281062372 1104 96-97 0.2804067467701117 1104 98-99 0.26340719488512576 1104 100-101 0.21222429332685167 1104 102-103 0.04117365007545715 1104 104-105 0.16794910278239428 1104 106-107 0.15117616512598175 1104 108-109 0.17220460054111797 1104 110-111 0.0615837586450958 1104 112-113 0.06263460950560429 1104 114-115 0.11474189194894535 1104 116-117 0.034773929519253954 1104 118-119 0.06257502621796718 1104 120-121 -0.06297541550449992 1104 122-123 -0.1847080367602345 1104 124-125 -0.19125954043625626 1105 1 0.37149854940814464 1105 2 0.26565986892612514 1105 3 0.21113695413771794 1105 4 0.295489180427424 1105 5 0.36850490298998295 1105 6 0.44243120177392825 1105 7 0.3712804093057187 1105 8 0.47750895712598407 1105 9 0.4951077502116732 1105 10-11 0.4964476205940187 1105 12-13 0.558755144164131 1105 14-15 0.6739335283271473 1105 16-17 0.5919645301172451 1105 18-19 0.515048954103932 1105 20-21 0.5794727726694262 1105 22-23 0.6120624874441205 1105 24-25 0.5424867398531887 1105 26-27 0.6121924016179179 1105 28-29 0.6180976816555344 1105 30-31 0.5883668493217726 1105 32-33 0.6915601590687999 1105 34-35 0.5459657175653092 1105 36-37 0.42989952634476225 1105 38-39 0.42827967150652313 1105 40-41 0.4545084408890929 1105 42-43 0.43031093818486 1105 44-45 0.41565070444726615 1105 46-47 0.46306908196591934 1105 48-49 0.30904443699265016 1105 50-51 0.3053152501984542 1105 52-53 0.354943418561696 1105 54-55 0.308504165335556 1105 56-57 0.3020088794663849 1105 58-59 0.20011897440938498 1105 60-61 0.20830316219688427 1105 62-63 0.19792680894859416 1105 64-65 0.11417940065686594 1105 66-67 0.14139917763287713 1105 68-69 0.1555735054637708 1105 70-71 0.2526636937724476 1105 72-73 0.17264149573625787 1105 74-75 0.2674795794379179 1105 76-77 0.2815788900056688 1105 78-79 0.4450652134551092 1105 80-81 0.394233703820845 1105 82-83 0.3752072454629456 1105 84-85 0.32685640078489797 1105 86-87 0.35969750228315434 1105 88-89 0.2743348630253841 1105 90-91 0.2761595968082169 1105 92-93 0.24357344299385986 1105 94-95 0.12705048785922557 1105 96-97 0.18900006807930225 1105 98-99 0.1761087873913887 1105 100-101 0.09932436615509133 1105 102-103 -0.053315414969471675 1105 104-105 0.06207593613437368 1105 106-107 0.10447296597660483 1105 108-109 0.1072030980819072 1105 110-111 0.12112661909963052 1105 112-113 0.04137623543111246 1105 114-115 -0.004427117459762542 1105 116-117 -0.022748776542105276 1105 118-119 -0.046363148841809476 1105 120-121 -0.18997869445004056 1105 122-123 -0.27576183853477687 1105 124-125 -0.4201543435018742 1106 1 0.3008340919974586 1106 2 0.2837083320615825 1106 3 0.2783288859740125 1106 4 0.2783654736524639 1106 5 0.28246796542158137 1106 6 0.16563594231529066 1106 7 0.21527635381600874 1106 8 0.276006855353927 1106 9 0.4377295899674749 1106 10-11 0.4318849045563269 1106 12-13 0.2979370036338409 1106 14-15 0.42286230149648674 1106 16-17 0.40506954402568596 1106 18-19 0.4533709227070659 1106 20-21 0.2539818339979476 1106 22-23 0.3955674616046281 1106 24-25 0.3366645511692141 1106 26-27 0.47572468914487587 1106 28-29 0.3684904509327822 1106 30-31 0.447397566605261 1106 32-33 0.3458370384424896 1106 34-35 0.31970673009724493 1106 36-37 0.30564325787547375 1106 38-39 0.3432102560079002 1106 40-41 0.32519869110767274 1106 42-43 0.3329970248740324 1106 44-45 0.3174702644989935 1106 46-47 0.23987868002493684 1106 48-49 0.19141868746731916 1106 50-51 0.2142891882917084 1106 52-53 0.2571214436228999 1106 54-55 0.21031672281500136 1106 56-57 0.12079302117443547 1106 58-59 0.22694332788856286 1106 60-61 0.2420643137932359 1106 62-63 0.23669479972386398 1106 64-65 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92-93 -2.2084878929961143 2216 94-95 -2.067317361543001 2216 96-97 -2.0266650871140754 2216 98-99 -2.1037383083773022 2216 100-101 -2.109441527295445 2216 102-103 -2.0560310901601966 2216 104-105 -2.160239084724612 2216 106-107 -1.9814482169539431 2216 108-109 -1.9999491337313167 2216 110-111 -2.0177456972717316 2216 112-113 -1.987674583617686 2216 114-115 -2.015583609413131 2216 116-117 -2.1182329280089824 2216 118-119 -2.054037907407011 2216 120-121 -1.951557909956307 2216 122-123 -2.0854749073219843 2216 124-125 -2.016742515628966 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33946.0 3 6508.0 4 4219.0 5 3508.0 6 3950.0 7 4555.0 8 5163.0 9 5877.0 10 6550.0 11 7605.0 12 8103.0 13 8243.0 14 7953.0 15 7448.0 16 7021.0 17 6763.0 18 6449.0 19 6369.0 20 6541.0 21 7151.0 22 8480.0 23 11109.0 24 15169.0 25 20733.0 26 24650.0 27 26786.0 28 32079.0 29 39168.0 30 46722.0 31 57739.0 32 73841.0 33 97320.0 34 135854.0 35 206907.0 36 398319.0 37 696296.0 38 261078.0 39 399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35009772020878 18.0357327193977 12.464249497519477 25.149920062874042 2 15.929967112465828 19.656729587290712 38.94577784329751 25.46752545694595 3 18.64908231921582 23.71888224733267 29.594744639220714 28.037290794230792 4 12.387939014370602 15.991103191274883 37.48959979535606 34.13135799899846 5 13.377460584252434 38.12640445296692 35.01815652511775 13.477978437662886 6 33.3654382384598 36.51186349242969 17.028753910481523 13.093944358628992 7 29.093608958989993 32.08414444333647 21.11458437205995 17.70766222561359 8 26.65575869981891 35.00347485509876 19.923817649662638 18.416948795419692 9 26.564324271830348 15.704307389627287 19.340874397536428 38.39049394100593 10-11 24.886395043717556 26.112437637311718 28.24764503965308 20.753522279317643 12-13 25.59390674119648 23.5022104451282 27.78614284803835 23.117739965636975 14-15 22.564123574393406 25.358217121412736 24.88183712382325 27.19582218037061 16-17 23.082644196346216 28.413355163742153 25.869533031427423 22.634467608484204 18-19 23.097027858904752 27.364425828053307 26.874997696796044 22.6635486162459 20-21 23.372686852623843 26.8879923678899 26.916346151136505 22.82297462834975 22-23 23.29315318998487 26.687976803657204 26.742104782836684 23.276765223521238 24-25 23.13659540504064 26.91490962125163 26.73971449393927 23.208780479768453 26-27 22.895918420876363 27.290299602808588 26.453949705851176 23.359832270463876 28-29 23.07955603207023 27.398471231871834 26.461861219485446 23.060111516572494 30-31 23.095785600077694 26.89784129494556 26.50812809334041 23.49824501163634 32-33 22.734479423316124 27.058567993039006 26.54861098767129 23.658341595973585 34-35 23.590211709946274 27.04411173040972 26.222799225857468 23.142877333786537 36-37 23.75597180141331 26.66605103379716 26.106345375675865 23.47163178911366 38-39 23.109182126865544 26.86103336212069 26.467916835749044 23.561867675264722 40-41 23.508394972771125 26.633678132664286 26.438365547987285 23.419561346577304 42-43 23.12646755850291 26.70709353181719 26.366563597959203 23.799875311720697 44-45 23.15768343825966 26.931324378213418 26.568182892203428 23.342809291323494 46-47 23.6406273472358 27.20373264278401 25.94805836112894 23.20758164885125 48-49 23.315909200280416 27.016098789068277 26.15716143140619 23.51083057924512 50-51 23.28272140766532 27.070313465312797 26.33708218823526 23.30988293878662 52-53 23.503453651870505 26.748806872966657 26.317490583314257 23.430248891848585 54-55 22.926626764853165 26.88546907753973 26.524163286718593 23.663740870888514 56-57 23.06100532869383 27.231832214861118 26.452059314072113 23.255103142372935 58-59 23.06916926902802 26.785440996740167 26.408667630006427 23.736722104225386 60-61 22.714883141792352 26.862322007356422 26.769196152185337 23.65359869866589 62-63 22.889144151340624 26.82953846607402 26.91091511444431 23.370402268141042 64-65 22.752416602501405 26.849000062431067 26.53469866605623 23.863884669011302 66-67 22.647538282780985 26.955261606229296 26.610608188959993 23.786591922029725 68-69 22.98189426695865 26.45398431481023 26.946829122998544 23.617292295232577 70-71 22.87347411611892 26.408586570661065 26.610231290959764 24.10770802226026 72-73 22.79520280804469 26.64807007625354 26.453274115592524 24.10345300010926 74-75 22.875635071810237 26.95221242467854 26.382157096990476 23.78999540652075 76-77 23.071490383031264 26.59400541415136 26.538207745947624 23.796296456869747 78-79 22.987224273771446 26.644830041762003 26.6897678214715 23.67817786299505 80-81 23.1757452630041 26.705690291328853 26.470490687071152 23.648073758595896 82-83 22.977877897927737 26.8247287521428 26.488297301351537 23.709096048577926 84-85 22.943469291180527 26.806212106727745 26.584301604055042 23.66601699803669 86-87 23.07158369090711 26.59986897993815 26.584347741638148 23.74419958751659 88-89 22.958760057213986 26.752438509921618 26.4719833127315 23.8168181201329 90-91 22.877728844921577 26.89684907667776 26.55736112881423 23.66806094958644 92-93 23.007855659970602 26.279164996606408 26.868897686526672 23.844081656896318 94-95 22.679589299591708 26.223110514741187 27.041975073134196 24.05532511253291 96-97 22.95348455084981 25.9456152801978 27.053625582955103 24.047274585997286 98-99 22.76182938664575 26.227171306812824 26.671166885499563 24.339832421041862 100-101 22.68023738643406 26.03990965667325 26.98628613619263 24.293566820700057 102-103 22.477975170216745 25.737006157912845 27.238212833092966 24.546805838777445 104-105 22.2878563781117 26.13103125806803 26.885749384417856 24.695362979402415 106-107 22.594268522398618 26.07533764148381 26.55038461396716 24.78000922215042 108-109 22.503753468951018 26.047492058427085 26.623139577885947 24.825614894735953 110-111 22.565250689721626 26.16881992190489 26.857902062672483 24.408027325701 112-113 22.454300339861618 26.292776975946953 27.040025978451588 24.21289670573984 114-115 22.32825784410213 26.544194475636353 26.735132299448082 24.392415380813436 116-117 22.288093119887346 26.318104409563798 26.983779110803578 24.41002335974528 118-119 22.054571526722324 26.63711543933432 26.611827951392502 24.696485082550854 120-121 22.136802851799185 26.74715083486408 26.79703520761416 24.31901110572257 122-123 22.072316194099933 26.743022490528865 26.77187595654334 24.41278535882786 124-125 22.03354742153351 26.859441428364562 26.698571791516766 24.408439358585163 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 396.0 1 338.0 2 276.0 3 234.5 4 181.0 5 163.5 6 149.0 7 130.0 8 129.0 9 123.0 10 115.0 11 113.0 12 122.0 13 129.5 14 152.0 15 176.5 16 197.5 17 245.0 18 317.0 19 415.0 20 538.0 21 736.5 22 1099.5 23 1720.5 24 2623.5 25 3982.5 26 6035.0 27 8978.0 28 12649.5 29 16642.0 30 20910.0 31 26713.0 32 35758.0 33 40850.5 34 41349.0 35 44516.0 36 51636.0 37 58171.5 38 61286.5 39 64241.5 40 66825.0 41 70665.0 42 74672.0 43 77313.5 44 80629.0 45 85966.0 46 87943.0 47 86911.0 48 85708.5 49 83831.5 50 83260.0 51 82356.0 52 83173.0 53 86387.0 54 91504.5 55 89160.0 56 79688.5 57 72549.0 58 67625.5 59 58992.0 60 46079.0 61 37655.5 62 32151.0 63 24726.0 64 17907.5 65 13204.0 66 9442.5 67 7693.0 68 5822.5 69 3864.0 70 2810.5 71 1812.5 72 1189.5 73 825.0 74 631.0 75 447.0 76 293.5 77 156.0 78 98.0 79 66.5 80 39.5 81 24.0 82 20.5 83 17.0 84 12.5 85 11.5 86 13.0 87 10.5 88 6.0 89 6.5 90 5.0 91 5.0 92 4.0 93 1.5 94 2.0 95 3.5 96 3.0 97 2.5 98 3.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04374458882904537 2 0.009798094227318387 3 0.0021677199617961035 4 0.0054626543037261804 5 0.02224080680802802 6 0.0255357411499581 7 0.002644618353391246 8 0.0 9 0.0 10-11 1.5174039732572726E-4 12-13 9.537967831902855E-4 14-15 3.901895931232986E-4 16-17 1.0838599808980517E-4 18-19 6.50315988538831E-5 20-21 2.1677199617961032E-5 22-23 2.1677199617961032E-5 24-25 0.0 26-27 2.1677199617961032E-5 28-29 3.468351938873765E-4 30-31 1.300631977077662E-4 32-33 1.7341759694368826E-4 34-35 8.237335854825193E-4 36-37 6.50315988538831E-5 38-39 1.300631977077662E-4 40-41 5.202527908310648E-4 42-43 8.237335854825193E-4 44-45 0.001387340775549506 46-47 6.50315988538831E-5 48-49 0.0 50-51 0.0 52-53 0.0011705687793698957 54-55 0.0032949343419300774 56-57 8.670879847184413E-5 58-59 5.202527908310648E-4 60-61 6.50315988538831E-4 62-63 5.419299904490259E-4 64-65 0.0011705687793698957 66-67 0.0022110743610320254 68-69 0.002774681551099012 70-71 0.0016908215702009606 72-73 0.002384491957975714 74-75 0.0017558531690548438 76-77 9.971511824262075E-4 78-79 0.0021026883629422202 80-81 8.454107851004803E-4 82-83 3.901895931232986E-4 84-85 4.552211919771817E-4 86-87 0.0023411375587397915 88-89 0.006156324691500933 90-91 3.901895931232986E-4 92-93 7.587019866286362E-4 94-95 0.0010188283820441687 96-97 1.7341759694368826E-4 98-99 7.153475873927142E-4 100-101 0.0015824355721111556 102-103 0.0011488915797519348 104-105 6.2863878892087E-4 106-107 0.007261861872016946 108-109 0.0016474671709650387 110-111 0.0010621827812800906 112-113 0.001907593566380571 114-115 0.002644618353391246 116-117 0.0016908215702009606 118-119 0.0469528143725036 120-121 0.010296669818531492 122-123 0.003858541531997064 124-125 0.0019509479656164932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2306571.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.5391817580165 #Duplication Level Percentage of deduplicated Percentage of total 1 82.36625057921398 55.62949168595864 2 10.398165207633415 14.045671398164734 3 3.309285183935673 6.705192405808274 4 1.413406214297415 3.8184119682137254 5 0.7382266296512034 2.4929611259310276 6 0.43796076157243913 1.7747706887232175 7 0.2735887476762309 1.2934572109377152 8 0.19185974256400978 1.036644002006153 9 0.14241921094596796 0.8656989276521719 >10 0.6647876761303384 8.043446622767787 >50 0.04475166424692539 2.1090944181264057 >100 0.018999182626117495 2.0740172516076187 >500 2.9919950628405455E-4 0.11114229410255157 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.335439923592207E-5 2 0.0 0.0 0.0 0.0 4.335439923592207E-5 3 0.0 0.0 0.0 0.0 4.335439923592207E-5 4 0.0 0.0 0.0 0.0 4.335439923592207E-5 5 0.0 0.0 0.0 0.0 4.335439923592207E-5 6 0.0 0.0 0.0 0.0 4.335439923592207E-5 7 0.0 0.0 0.0 0.0 4.335439923592207E-5 8 0.0 0.0 0.0 0.0 8.670879847184414E-5 9 0.0 0.0 0.0 0.0 8.670879847184414E-5 10-11 0.0 0.0 0.0 0.0 1.300631977077662E-4 12-13 4.335439923592207E-5 0.0 0.0 0.0 5.636071900669869E-4 14-15 4.335439923592207E-5 0.0 0.0 0.0 6.2863878892087E-4 16-17 4.335439923592207E-5 0.0 0.0 0.0 7.370247870106751E-4 18-19 4.335439923592207E-5 0.0 0.0 6.50315988538831E-5 8.237335854825193E-4 20-21 4.335439923592207E-5 0.0 0.0 1.300631977077662E-4 0.0010621827812800906 22-23 4.335439923592207E-5 0.0 0.0 1.5174039732572726E-4 0.0011272143801339739 24-25 2.1677199617961033E-4 0.0 0.0 1.7341759694368828E-4 0.0011272143801339739 26-27 2.1677199617961033E-4 0.0 0.0 8.237335854825193E-4 0.0012139231786058179 28-29 2.601263954155324E-4 0.0 0.0 0.0032949343419300774 0.0012139231786058179 30-31 3.0348079465145446E-4 0.0 0.0 0.013851730555877101 0.00125727757784174 32-33 3.0348079465145446E-4 0.0 0.0 0.03485693698568135 0.0013439863763135842 34-35 3.0348079465145446E-4 0.0 0.0 0.066202167633253 0.0014306951747854282 36-37 3.0348079465145446E-4 0.0 0.0 0.11829247831521336 0.0014306951747854282 38-39 3.4683519388737657E-4 0.0 0.0 0.1967205865329964 0.0015390811728752335 40-41 3.4683519388737657E-4 0.0 0.0 0.27924568547857403 0.0015607583724931945 42-43 4.1186679274125967E-4 0.0 0.0 0.36556429435729487 0.0015824355721111554 44-45 5.202527908310648E-4 0.0 0.0 0.47191263568301167 0.0016041127717291166 46-47 7.370247870106751E-4 0.0 0.0 0.6025827949800808 0.0016257899713470777 48-49 8.237335854825193E-4 0.0 0.0 0.7411434549380878 0.0016908215702009606 50-51 8.237335854825193E-4 0.0 0.0 0.8975444501816766 0.0018208847679087269 52-53 8.237335854825193E-4 0.0 0.0 1.0420229856353869 0.001864239167144649 54-55 8.237335854825193E-4 0.0 0.0 1.2002448656468845 0.002037656764088337 56-57 9.971511824262075E-4 0.0 0.0 1.3788216360996475 0.0021026883629422202 58-59 9.971511824262075E-4 0.0 0.0 1.5810482313356058 0.0022110743610320254 60-61 9.971511824262075E-4 0.0 0.0 1.80139696545218 0.0022544287602679477 62-63 9.971511824262075E-4 0.0 0.0 2.0142237113013213 0.002384491957975714 64-65 9.971511824262075E-4 0.0 0.0 2.2378240253605894 0.00251455515568348 66-67 0.0010405055816621296 0.0 0.0 2.496888237994842 0.00251455515568348 68-69 0.0010405055816621296 0.0 0.0 2.792716981181156 0.00251455515568348 70-71 0.0010405055816621296 0.0 0.0 3.108705520012174 0.002536232355301441 72-73 0.0010621827812800906 0.0 0.0 3.43247617350604 0.002644618353391246 74-75 0.0010838599808980518 0.0 0.0 3.7492234143236867 0.0027746815510990125 76-77 0.0010838599808980518 0.0 0.0 4.0886883603409565 0.0027746815510990125 78-79 0.0010838599808980518 0.0 0.0 4.483516874182499 0.0028613903495708563 80-81 0.00125727757784174 0.0 0.0 4.904661508360245 0.0028613903495708563 82-83 0.00125727757784174 0.0 0.0 5.348415461739526 0.0028830675491888173 84-85 0.0013439863763135842 0.0 0.0 5.791887611523773 0.0029047447488067786 86-87 0.0014306951747854282 0.0 0.0 6.247759119489494 0.0029264219484247396 88-89 0.0014306951747854282 0.0 0.0 6.737642153655795 0.0029480991480427005 90-91 0.0014740495740213503 0.0 0.0 7.271790029441973 0.0029480991480427005 92-93 0.0014740495740213503 0.0 0.0 7.8131130583016954 0.0030781623457504666 94-95 0.0014740495740213503 0.0 0.0 8.369415032097429 0.0030781623457504666 96-97 0.0014740495740213503 0.0 0.0 8.925391847898894 0.003316611541548038 98-99 0.0015607583724931945 0.0 0.0 9.501528459345064 0.003381643140401921 100-101 0.0016257899713470775 0.0 0.0 10.145146193201944 0.003381643140401921 102-103 0.0016908215702009606 0.0 0.0 10.798496989687289 0.0034683519388737654 104-105 0.002037656764088337 0.0 0.0 11.462079424392313 0.0035550607373456096 106-107 0.0020810111633242593 0.0 0.0 12.12052436278788 0.0035550607373456096 108-109 0.0020810111633242593 0.0 0.0 12.813024181783263 0.0035984151365815314 110-111 0.002124365562560181 0.0 0.0 13.54801131202985 0.0036851239350533757 112-113 0.002124365562560181 0.0 0.0 14.311200478979401 0.0036851239350533757 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2530 0.0 44.241585 1 ATCAACG 2620 0.0 41.797443 3 CAACGCA 2620 0.0 41.342228 5 GGTATCA 1675 0.0 40.521305 1 TCAACGC 2820 0.0 38.62204 4 AACGCAG 2865 0.0 38.218174 6 GTACATG 6325 0.0 37.55828 1 TACATGG 6455 0.0 35.31309 2 TATCAAC 3255 0.0 34.74048 2 ACATGGG 6880 0.0 32.612656 3 ACGCAGA 3450 0.0 31.903961 7 CGCAGAG 3435 0.0 31.522291 8 CATGGGG 4695 0.0 26.24025 4 CATGGGA 2920 0.0 23.643314 4 TAAGGTG 1520 0.0 22.709534 5 TATTCCG 410 1.8189894E-12 21.77365 5 TAAGGTA 875 0.0 21.085642 4 GCAGAGT 5040 0.0 20.893724 9 GTAAGGT 1355 0.0 20.20467 4 AGGTAAG 1150 0.0 20.183664 2 >>END_MODULE