FastQCFastQC Report
Thu 26 May 2016
SRR1364370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306571
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.10682523971731198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.10235973659601201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.065.065831
GTATCAA49650.050.9830931
ATCAACG57950.042.541153
CAACGCA59750.041.2595755
TCAACGC60600.040.6808474
TATCAAC64250.038.5551572
AACGCAG63850.038.4910246
ACGCAGA73950.033.2282037
GTACATG63400.032.7863241
CGCAGAG74600.032.779178
TACATGG64350.032.202752
ACATGGG65750.030.5208363
TAAGGTG16650.028.2536895
GTGGTAT13600.028.028351
GCAGAGT89850.026.818349
GTAAGGT15100.026.8160154
AGGTAAG14250.025.4904352
GAGTACT59300.024.68171912-13
CAGAGTA84700.024.62065310-11
GTACTTT58800.024.5880414-15
Produced by FastQC (version 0.11.5)