FastQCFastQC Report
Thu 26 May 2016
SRR1364369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964936
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.057.684951
GTATCAA25150.047.1062931
TCAACGC28050.040.3126034
ATCAACG28200.039.8871273
CAACGCA28800.039.2607965
AACGCAG30500.037.067776
GTACATG78250.036.74741
AGGTAAG14350.035.6670042
TACATGG81450.034.9997182
ACATGGG81700.034.018483
TATCAAC33300.033.957012
TAAGGTA9050.033.5383454
TAAGGTG16850.033.199075
GTGTAAG9650.032.0804021
GGTAAGG18200.029.7570653
ACGCAGA38050.029.238317
GTAAGGT19400.029.1435174
CGCAGAG40400.027.6841248
CATGGGA46650.027.6839584
CATGGGG44200.027.6027074
Produced by FastQC (version 0.11.5)