##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364369_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1964936 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12597102399264 31.0 31.0 34.0 28.0 34.0 2 31.164780430507662 33.0 31.0 34.0 28.0 34.0 3 31.306082233721607 33.0 31.0 34.0 28.0 34.0 4 34.71826257954458 37.0 35.0 37.0 32.0 37.0 5 34.797700790254744 37.0 35.0 37.0 32.0 37.0 6 34.85594848890753 37.0 35.0 37.0 32.0 37.0 7 34.78718848858182 37.0 35.0 37.0 32.0 37.0 8 34.70909688661615 37.0 35.0 37.0 32.0 37.0 9 36.24754750281943 39.0 37.0 39.0 32.0 39.0 10-11 36.15285688694186 39.0 37.0 39.0 31.5 39.0 12-13 36.16237144619468 39.0 37.0 39.0 32.0 39.0 14-15 37.47093391336919 40.0 38.0 41.0 32.0 41.0 16-17 37.4701092045746 40.0 38.0 41.0 31.5 41.0 18-19 37.475225401743366 40.0 38.0 41.0 31.0 41.0 20-21 37.494422973572675 40.0 38.0 41.0 31.0 41.0 22-23 37.47115554908659 40.0 38.0 41.0 31.0 41.0 24-25 37.475011145401176 40.0 38.0 41.0 31.0 41.0 26-27 37.42956818949828 40.0 38.0 41.0 31.0 41.0 28-29 37.4253382807379 40.0 38.0 41.0 31.0 41.0 30-31 37.30611785829157 40.0 37.5 41.0 30.5 41.0 32-33 37.25793664526478 40.0 37.0 41.0 30.0 41.0 34-35 37.246315147160004 40.0 37.0 41.0 30.0 41.0 36-37 37.17190839803433 40.0 37.0 41.0 30.0 41.0 38-39 37.06496089440063 40.0 37.0 41.0 30.0 41.0 40-41 36.99428225652133 40.0 37.0 41.0 30.0 41.0 42-43 36.84119279202987 40.0 36.5 41.0 30.0 41.0 44-45 36.71637982102216 40.0 36.0 41.0 30.0 41.0 46-47 36.59281854472614 40.0 36.0 41.0 30.0 41.0 48-49 36.419251822960135 40.0 35.5 41.0 30.0 41.0 50-51 36.273380914187534 39.5 35.0 41.0 29.5 41.0 52-53 36.07277158136448 39.0 35.0 41.0 29.0 41.0 54-55 35.860261352023684 39.0 35.0 41.0 29.0 41.0 56-57 35.635667268552254 39.0 35.0 41.0 28.0 41.0 58-59 35.37406256488761 38.0 35.0 40.0 28.0 41.0 60-61 35.08861179193622 38.0 34.0 40.0 28.0 41.0 62-63 34.79697150441541 37.0 34.0 40.0 28.0 41.0 64-65 34.41552269386891 37.0 34.0 40.0 27.0 41.0 66-67 34.09124571996238 36.0 34.0 39.0 26.0 41.0 68-69 33.7531744036447 36.0 33.0 39.0 26.0 41.0 70-71 33.374236107435564 35.0 33.0 38.5 26.0 40.5 72-73 33.03625537931006 35.0 33.0 37.5 26.0 40.0 74-75 32.697489892800576 35.0 33.0 37.0 25.5 39.0 76-77 32.49396519784868 35.0 33.0 36.5 25.5 39.0 78-79 32.34129254082576 35.0 33.0 36.0 25.5 39.0 80-81 32.11062039679664 35.0 33.0 36.0 25.0 37.5 82-83 31.835882186493606 35.0 33.0 35.5 25.0 37.0 84-85 31.612662702500234 35.0 33.0 35.0 24.0 37.0 86-87 31.439256800221482 35.0 32.5 35.0 24.0 36.0 88-89 31.1885829869268 35.0 32.0 35.0 23.0 36.0 90-91 31.06930022148304 35.0 32.0 35.0 23.0 36.0 92-93 30.920982668137793 35.0 32.0 35.0 20.5 35.5 94-95 30.811390040184513 35.0 32.0 35.0 20.0 35.0 96-97 30.707234230529643 35.0 32.0 35.0 20.0 35.0 98-99 30.557177943709107 35.0 32.0 35.0 19.0 35.0 100-101 30.448625298737465 35.0 32.0 35.0 18.0 35.0 102-103 29.41590718476327 33.5 29.5 34.5 15.5 35.0 104-105 30.11262631454663 34.0 31.0 35.0 16.5 35.0 106-107 30.450840638066584 35.0 32.0 35.0 18.0 35.0 108-109 30.480878512073676 35.0 32.0 35.0 16.0 35.0 110-111 30.37833649543802 35.0 32.0 35.0 11.5 35.0 112-113 30.260557850230235 35.0 32.0 35.0 6.0 35.0 114-115 30.128607751092147 35.0 32.0 35.0 2.0 35.0 116-117 29.97349379318207 35.0 32.0 35.0 2.0 35.0 118-119 29.767324737294242 35.0 31.5 35.0 2.0 35.0 120-121 29.560743708700947 35.0 31.0 35.0 2.0 35.0 122-123 29.35822006416494 35.0 31.0 35.0 2.0 35.0 124-125 28.5122324594796 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17300574442032612 1101 2 0.07956664838278726 1101 3 0.18068387359187454 1101 4 0.17214316473037172 1101 5 0.1542512802431517 1101 6 0.19791220708623314 1101 7 0.26849551288233187 1101 8 0.11872022552859818 1101 9 0.33943486353928876 1101 10-11 0.3785889486681313 1101 12-13 0.38916080038800516 1101 14-15 0.43415625024105253 1101 16-17 0.38706019719943896 1101 18-19 0.2987806142584617 1101 20-21 0.3955272919310744 1101 22-23 0.4777389531573206 1101 24-25 0.48745061799104406 1101 26-27 0.5377367484760782 1101 28-29 0.5326792110695209 1101 30-31 0.521100929098246 1101 32-33 0.41579178138335493 1101 34-35 0.42298266514782057 1101 36-37 0.4119292791448501 1101 38-39 0.5699485463357448 1101 40-41 0.40866075856897055 1101 42-43 0.49732343916102906 1101 44-45 0.4646348603304489 1101 46-47 0.44261515330268963 1101 48-49 0.42901553123868297 1101 50-51 0.3343217679571211 1101 52-53 0.3568671191562771 1101 54-55 0.35591862048136846 1101 56-57 0.29318700843410284 1101 58-59 0.38272272368614324 1101 60-61 0.29151647717531404 1101 62-63 0.33619490212407754 1101 64-65 0.4815648551606557 1101 66-67 0.41561093682391004 1101 68-69 0.3257972402509921 1101 70-71 0.2545928865167184 1101 72-73 0.33695138692444004 1101 74-75 0.3375571527587482 1101 76-77 0.3245401757892452 1101 78-79 0.4345308043116418 1101 80-81 0.5116984592444354 1101 82-83 0.391415472622036 1101 84-85 0.3356474756648602 1101 86-87 0.37838390314849946 1101 88-89 0.37310450949841467 1101 90-91 0.3558939050479779 1101 92-93 0.2137191926825679 1101 94-95 0.1724493318954643 1101 96-97 0.2427533428810058 1101 98-99 0.13881927074293898 1101 100-101 0.1664261576799646 1101 102-103 -0.032176079697809 1101 104-105 0.13750180288231562 1101 106-107 0.1673717697969721 1101 108-109 0.26511411477309466 1101 110-111 0.21942617803287234 1101 112-113 0.2174198121526807 1101 114-115 0.07153000816402866 1101 116-117 0.05182459293708774 1101 118-119 0.10448894332924752 1101 120-121 -0.08415434015740786 1101 122-123 -0.10969067337603633 1101 124-125 -0.30641503920384494 1102 1 0.16806152535338725 1102 2 0.19049879682158277 1102 3 0.25166670425947046 1102 4 0.22981255809541068 1102 5 0.24916061198037198 1102 6 0.24933360945158967 1102 7 0.1745186343977494 1102 8 0.21943002612491824 1102 9 0.25297303177436703 1102 10-11 0.36811720768230316 1102 12-13 0.2851721727153418 1102 14-15 0.29798724759194073 1102 16-17 0.42250104114583564 1102 18-19 0.42355061835959873 1102 20-21 0.405677939244832 1102 22-23 0.31023959049534966 1102 24-25 0.333812664788276 1102 26-27 0.2828992142538027 1102 28-29 0.296030403722952 1102 30-31 0.38141063381677043 1102 32-33 0.3995826125829893 1102 34-35 0.3007173302405377 1102 36-37 0.20647886127278525 1102 38-39 0.27724274157177575 1102 40-41 0.2546618780670826 1102 42-43 0.13638748054921024 1102 44-45 0.16377078236460818 1102 46-47 0.12924089178348908 1102 48-49 0.1955014571522966 1102 50-51 0.07019572466247581 1102 52-53 0.13144412067480715 1102 54-55 0.189823479768215 1102 56-57 0.17661580392064025 1102 58-59 0.09680110518006302 1102 60-61 0.05924936880567344 1102 62-63 -0.03768144321337985 1102 64-65 -0.10551907394518878 1102 66-67 -0.09200594542927831 1102 68-69 -0.11495422285544521 1102 70-71 -0.14119507111970364 1102 72-73 -0.11187591109482042 1102 74-75 -0.035459132613510747 1102 76-77 0.0018749805421265364 1102 78-79 0.07172855367707598 1102 80-81 -0.01771780839319348 1102 82-83 0.04744032603429105 1102 84-85 0.020972213247851812 1102 86-87 -0.03978397168939196 1102 88-89 -0.04325699694701157 1102 90-91 -0.07789013711608561 1102 92-93 -0.09861142780757248 1102 94-95 -0.16138659789759657 1102 96-97 -0.18931196487013224 1102 98-99 -0.184808512761645 1102 100-101 -0.19360505802848138 1102 102-103 -0.3395434302558904 1102 104-105 -0.11609569321052149 1102 106-107 -0.11936141556309465 1102 108-109 -0.1496637452120524 1102 110-111 -0.19117019024875148 1102 112-113 -0.13700317524465078 1102 114-115 -0.19220428006794066 1102 116-117 -0.08743064986099114 1102 118-119 -0.1777634785552813 1102 120-121 -0.23811823634335738 1102 122-123 -0.21217913201556726 1102 124-125 -0.26499153085194393 1103 1 0.26714791807821214 1103 2 0.2715964829295814 1103 3 0.35096326047699833 1103 4 0.35373723881254193 1103 5 0.298544086388965 1103 6 0.3391810908025974 1103 7 0.18473899892961043 1103 8 0.2847282949391001 1103 9 0.4227823731102447 1103 10-11 0.4973188670881541 1103 12-13 0.3541127002429576 1103 14-15 0.4152916632569017 1103 16-17 0.34514396612472353 1103 18-19 0.2915212419435562 1103 20-21 0.270407363238121 1103 22-23 0.3318460540062773 1103 24-25 0.33615805255659836 1103 26-27 0.4030932916694212 1103 28-29 0.365058068002476 1103 30-31 0.47197827310212404 1103 32-33 0.48308228221104343 1103 34-35 0.43190173898518225 1103 36-37 0.38327369431072356 1103 38-39 0.3858345748508114 1103 40-41 0.4515651701913441 1103 42-43 0.3634683291910008 1103 44-45 0.33336990269177136 1103 46-47 0.36379128684944817 1103 48-49 0.42886420417204363 1103 50-51 0.38799271892191456 1103 52-53 0.36991779766858457 1103 54-55 0.3779977974443085 1103 56-57 0.3041898753361991 1103 58-59 0.30709078800458656 1103 60-61 0.19573629334652054 1103 62-63 0.20514976156753306 1103 64-65 0.28237095360727693 1103 66-67 0.25661042647935517 1103 68-69 0.20541690033556392 1103 70-71 0.17139471136855633 1103 72-73 0.1759019832487283 1103 74-75 0.16612579000214822 1103 76-77 0.1812023584379432 1103 78-79 0.224523337021175 1103 80-81 0.20488852490148446 1103 82-83 0.15294855358418147 1103 84-85 0.0825326317435291 1103 86-87 0.16315263462687923 1103 88-89 0.2276421922153169 1103 90-91 0.16989768218978085 1103 92-93 0.20244766045787443 1103 94-95 0.09049342785117176 1103 96-97 0.03328280770697134 1103 98-99 0.015993444439082793 1103 100-101 -0.005484862915775324 1103 102-103 -0.23036079254416464 1103 104-105 -0.03340458362090004 1103 106-107 -0.04833944122719913 1103 108-109 -0.040295503342974826 1103 110-111 0.03046791856969122 1103 112-113 -0.012868100970781171 1103 114-115 -0.04302759871398365 1103 116-117 -0.04089312503708342 1103 118-119 -0.0923979321163948 1103 120-121 -0.23595776254028777 1103 122-123 -0.14681374202022468 1103 124-125 -0.23208213650931242 1104 1 0.3636368954745173 1104 2 0.39799048610370136 1104 3 0.4034549795280995 1104 4 0.49228690886653936 1104 5 0.45186766285225843 1104 6 0.4197396395025592 1104 7 0.5373862733448647 1104 8 0.5034409955370691 1104 9 0.6922864046755421 1104 10-11 0.6584931389607647 1104 12-13 0.5156042932722258 1104 14-15 0.5718619451332856 1104 16-17 0.6284712439126423 1104 18-19 0.5893641493850481 1104 20-21 0.5382920369435737 1104 22-23 0.5700800688396797 1104 24-25 0.6036678339812553 1104 26-27 0.7230698597685858 1104 28-29 0.6574852665961117 1104 30-31 0.758014170284639 1104 32-33 0.6034369837999591 1104 34-35 0.5593992264815952 1104 36-37 0.46378912133206285 1104 38-39 0.441556849934706 1104 40-41 0.6028478616368744 1104 42-43 0.5592400701100644 1104 44-45 0.5017032798456498 1104 46-47 0.465773943434975 1104 48-49 0.4582798753946875 1104 50-51 0.3235664378397445 1104 52-53 0.30015491136973793 1104 54-55 0.378423914426115 1104 56-57 0.3042400234118432 1104 58-59 0.30116708198359987 1104 60-61 0.23412631686002072 1104 62-63 0.44941763819488045 1104 64-65 0.5030626615974683 1104 66-67 0.4372824401586115 1104 68-69 0.36544824939926457 1104 70-71 0.3119856630917752 1104 72-73 0.24280971577601207 1104 74-75 0.26686046781011186 1104 76-77 0.2937381533977046 1104 78-79 0.3479447775957638 1104 80-81 0.3356198549712488 1104 82-83 0.28838977958620404 1104 84-85 0.2433321813288103 1104 86-87 0.30167391577024816 1104 88-89 0.2884420024572627 1104 90-91 0.23896563515553026 1104 92-93 0.2840877076571289 1104 94-95 0.22417210482140604 1104 96-97 0.21473877463666824 1104 98-99 0.2088860058418618 1104 100-101 0.18113454254223882 1104 102-103 0.16361353934747314 1104 104-105 0.2138080900047683 1104 106-107 0.2190739538961175 1104 108-109 0.1264821501758675 1104 110-111 0.03813598432044785 1104 112-113 0.1273626912396182 1104 114-115 0.18266724395591893 1104 116-117 0.07912776602792704 1104 118-119 0.1606250052167546 1104 120-121 0.1104540944820549 1104 122-123 0.03702542012499421 1104 124-125 -0.1681022582971714 1105 1 -0.02702686690811973 1105 2 -0.09368633388486103 1105 3 -0.13278631906755578 1105 4 0.01910514659703466 1105 5 -0.02871270611511534 1105 6 0.0434484018268364 1105 7 0.002835873212703177 1105 8 0.044613800736044595 1105 9 0.030967104325462458 1105 10-11 -0.005762282105052918 1105 12-13 0.1106323155427944 1105 14-15 0.20020154254995504 1105 16-17 0.09691308086630812 1105 18-19 0.05508411792396828 1105 20-21 0.14192662689451652 1105 22-23 0.2211515099170427 1105 24-25 0.15360668958440726 1105 26-27 0.23616427803037254 1105 28-29 0.209700651100988 1105 30-31 0.25643034185527824 1105 32-33 0.2641731228320765 1105 34-35 0.0662972751582771 1105 36-37 0.0048507038855305495 1105 38-39 0.05429037259720104 1105 40-41 0.09785743284494686 1105 42-43 0.10614953959039042 1105 44-45 0.07601134255591546 1105 46-47 0.03403602843197007 1105 48-49 -0.09311957708911223 1105 50-51 -0.030983522340079617 1105 52-53 0.08192317410568961 1105 54-55 0.029068085461624094 1105 56-57 -0.06307472747187859 1105 58-59 0.00794109103584617 1105 60-61 -0.10204452202135883 1105 62-63 -0.17688717964239942 1105 64-65 -0.1705446647762372 1105 66-67 -0.17820617180970544 1105 68-69 -0.29822086783187274 1105 70-71 -0.24591094272080483 1105 72-73 -0.24764219608627513 1105 74-75 -0.17486593660305516 1105 76-77 -0.2791895588411393 1105 78-79 -0.09230486842217545 1105 80-81 -0.015539329662381363 1105 82-83 -0.050085870847418335 1105 84-85 -0.08952745501915516 1105 86-87 -0.12488692739392349 1105 88-89 -0.10427752702717541 1105 90-91 -0.09546492285667085 1105 92-93 -0.17948558526044778 1105 94-95 -0.181910640337545 1105 96-97 -0.21501306979618207 1105 98-99 -0.2629120389740187 1105 100-101 -0.32793835217357525 1105 102-103 -0.5265182622932798 1105 104-105 -0.3910579175713913 1105 106-107 -0.40009331059694375 1105 108-109 -0.28059654831940506 1105 110-111 -0.34434915382009734 1105 112-113 -0.2462223906049843 1105 114-115 -0.24958896184121926 1105 116-117 -0.34177587363494766 1105 118-119 -0.2900251366005371 1105 120-121 -0.2650832758779096 1105 122-123 -0.38950556913029644 1105 124-125 -0.3592449402286917 1106 1 0.1835512656547671 1106 2 0.1680853805486784 1106 3 0.25069697352400055 1106 4 0.28303540169282115 1106 5 0.22123836739097413 1106 6 0.19490418692755895 1106 7 0.22686307014060958 1106 8 0.2862375315079362 1106 9 0.3844043963878647 1106 10-11 0.41075163408702053 1106 12-13 0.29019821616137875 1106 14-15 0.3118430857739227 1106 16-17 0.26112135608494924 1106 18-19 0.3341490219392753 1106 20-21 0.298833946319391 1106 22-23 0.34004567062822133 1106 24-25 0.38760195114704743 1106 26-27 0.3889919834088289 1106 28-29 0.3506886261986324 1106 30-31 0.4192159522686296 1106 32-33 0.41085036688983934 1106 34-35 0.367338302616794 1106 36-37 0.41472366397156435 1106 38-39 0.4064598000727315 1106 40-41 0.42546961033379915 1106 42-43 0.3179988519779826 1106 44-45 0.24659203961569176 1106 46-47 0.3526091030113392 1106 48-49 0.3513293352027418 1106 50-51 0.24796534835088124 1106 52-53 0.3812147184739487 1106 54-55 0.300511738930723 1106 56-57 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2216 38-39 -1.3256122516966187 2216 40-41 -1.2654265237702447 2216 42-43 -1.2004477732719678 2216 44-45 -1.157534753392099 2216 46-47 -1.0545146518927524 2216 48-49 -1.1105663934852288 2216 50-51 -1.12357060938362 2216 52-53 -1.057065620356319 2216 54-55 -1.0282137618568754 2216 56-57 -1.2142158465109745 2216 58-59 -1.3036280347581624 2216 60-61 -1.3451618135812708 2216 62-63 -1.3502853871430531 2216 64-65 -1.202860089229901 2216 66-67 -1.3209028528498834 2216 68-69 -1.2635024726376543 2216 70-71 -1.2214843322268507 2216 72-73 -1.235735002723871 2216 74-75 -1.2157152306573877 2216 76-77 -1.2460783139854712 2216 78-79 -1.3092605965565483 2216 80-81 -1.2232520801917204 2216 82-83 -1.1652636020563065 2216 84-85 -1.163097637072891 2216 86-87 -1.2648324788565724 2216 88-89 -1.2124158430703993 2216 90-91 -1.3047304396424195 2216 92-93 -1.2799861605231833 2216 94-95 -1.2124948381763616 2216 96-97 -1.1358006399315066 2216 98-99 -1.265869928774947 2216 100-101 -1.1908134210600778 2216 102-103 -1.1914258053251885 2216 104-105 -1.361593931113429 2216 106-107 -1.1709596772246158 2216 108-109 -1.0674218231403003 2216 110-111 -1.0276243163277918 2216 112-113 -1.0397190816357202 2216 114-115 -1.0665360690914625 2216 116-117 -1.194695835574148 2216 118-119 -1.1396432797748908 2216 120-121 -0.9034435313461273 2216 122-123 -0.9455852260996771 2216 124-125 -0.8916199857501823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28861.0 3 5505.0 4 3726.0 5 3138.0 6 3532.0 7 3953.0 8 4532.0 9 5156.0 10 5862.0 11 6686.0 12 7486.0 13 7519.0 14 7293.0 15 6902.0 16 6613.0 17 5632.0 18 5711.0 19 5761.0 20 6031.0 21 6358.0 22 7786.0 23 9811.0 24 13693.0 25 18640.0 26 21839.0 27 23347.0 28 27389.0 29 33619.0 30 39991.0 31 49152.0 32 63382.0 33 83898.0 34 117139.0 35 178296.0 36 349754.0 37 579254.0 38 211234.0 39 455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.83862575155326 17.731387267138775 12.472487384800868 24.957499596507102 2 15.915173095461316 19.65324921084268 38.366889697132564 26.064687996563435 3 18.748718115753572 23.984047888905234 29.334753605943565 27.93248038939763 4 12.651628894102798 15.87496119257137 36.588254454583854 34.88515545874198 5 13.305139402664 38.574356995636016 34.353259295419825 13.767244306280158 6 33.36418291869658 36.16518273034103 16.77733828148465 13.693296069477745 7 29.45297402717037 32.46412850578234 20.385207910381446 17.697689556665843 8 27.733034900462155 33.746935643032764 19.94436445859931 18.57566499790578 9 27.105310300182804 15.551142632635361 18.980363737037745 38.36318333014408 10-11 25.054302078339525 26.264927982313907 27.454662599354485 21.22610733999208 12-13 25.88753861809254 23.67924914799092 27.18881661263124 23.244395621285303 14-15 22.51925898519385 25.261167463218737 24.31726471263309 27.90230883895432 16-17 23.118063687732544 28.481559912281906 25.624284007937177 22.776092392048373 18-19 23.52715154809271 27.11284450266523 26.56096846000934 22.799035489232715 20-21 23.4194710208045 26.58939695476009 26.736908158054046 23.254223866381366 22-23 23.587491803166056 26.570668604643764 26.625275995064467 23.21656359712571 24-25 23.27447306171804 26.837871564264688 26.533408721709 23.35424665230827 26-27 22.625377048479518 27.147439632801728 26.29954812708627 23.927635191632486 28-29 23.284816724883374 27.54278336336411 26.00030993494165 23.17208997681086 30-31 22.84796404960991 26.90337569277277 25.996116910017154 24.25254334760017 32-33 22.432500861988895 27.174063231179694 25.9808161349054 24.412619771926007 34-35 23.897148914827763 27.08929148969792 25.638722420293767 23.37483717518055 36-37 24.036125386399746 26.2032210751099 25.73694587197331 24.023707666517044 38-39 23.128032707501507 26.73350190579163 25.826116584725728 24.312348801981138 40-41 23.640046414103374 26.440262198969933 26.244554485678805 23.675136901247885 42-43 23.34899130753415 26.438582741282087 25.8582537711459 24.354172180037864 44-45 23.058550682743107 26.80786776151207 26.493068747107046 23.640512808637773 46-47 24.05589704285233 27.235342255770423 25.30051899962034 23.408241701756904 48-49 23.293430422161332 27.159357862037236 25.441337529568393 24.105874186233038 50-51 23.55567814931377 27.23304982961277 25.737148690847945 23.474123330225513 52-53 24.06431019372858 26.553808461846167 25.91087956012324 23.471001784302008 54-55 22.82432724728036 27.094446211591066 26.27441949233412 23.806807048794454 56-57 23.170153893287964 27.251692551349077 26.041574954464096 23.53657860089886 58-59 22.972256348610987 26.429576196055 26.136663209355298 24.461504245978713 60-61 22.319212687587868 26.889059945417415 26.61767381499176 24.174053552002952 62-63 22.826850070371876 26.869231471941458 26.818186097794893 23.485732359891774 64-65 22.47221489926724 27.13728216470847 26.209831330010353 24.18067160601394 66-67 22.387406438163815 27.14416729393239 26.324065068704044 24.144361199199746 68-69 23.34559286628016 26.238615314890513 26.789543609982463 23.626248208846864 70-71 23.062195307525965 26.462027664638903 26.02587115519385 24.44990587264128 72-73 22.745525047063918 26.676831828492414 26.109571012188525 24.468072112255143 74-75 22.700693651532415 27.244455991590378 26.231066702665263 23.82378365421195 76-77 23.16053850000191 26.579512214982675 26.389731806031087 23.870217478984326 78-79 23.155745683132224 26.7532181998947 26.361972833448565 23.729063283524514 80-81 23.532914399278337 26.73459183193102 26.07196521973681 23.660528549053836 82-83 23.135510282601693 26.767055774761428 26.285967731018197 23.811466211618686 84-85 22.78258147914419 27.21828369602834 26.37407629827169 23.62505852655579 86-87 23.316783649377076 27.23124915548772 25.825980722090762 23.625986473044442 88-89 22.83896313491293 27.326865756340318 25.74787137693837 24.08629973180838 90-91 22.67416517742294 27.239559199124034 26.03610267778873 24.050172945664297 92-93 23.091768164209213 26.52012206169449 26.56903020395866 23.819079570137635 94-95 22.523191925525264 26.277820640966397 27.00668428241933 24.19230315108901 96-97 23.11370471005734 25.70344995707211 26.848940136585504 24.333905196285045 98-99 22.674856666937245 26.349160384168997 26.404608032962212 24.571374915931543 100-101 22.558992643922913 26.026461316724564 26.976863015329993 24.437683024022526 102-103 22.207898620794953 25.584406331110998 27.26510254974808 24.942592498345974 104-105 21.969503037532885 26.369765236903138 26.614279381775415 25.046452343788566 106-107 22.453926373810944 26.226823222838064 26.15218420289191 25.167066200459082 108-109 22.509351668541242 25.913053656947717 26.37384281053901 25.20375186397203 110-111 22.564671263001305 26.152769175413088 26.68887815607825 24.593681405507358 112-113 22.47049252538382 26.458617012153645 26.931545814293823 24.13934464816871 114-115 22.37168026293758 26.889469417169625 26.44521769047121 24.293632629421587 116-117 22.33474146374482 26.646872599271664 26.657611067441906 24.360774869541608 118-119 21.951318237407985 27.1028867724135 26.23644263775787 24.709352352420648 120-121 21.93374198280333 27.391594470965547 26.396853843597164 24.277809702633963 122-123 21.94050489761476 27.342335281250225 26.42892478448561 24.288235036649404 124-125 21.839509546908026 27.28977437264521 26.445014115387377 24.42570196505939 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 695.0 1 607.5 2 483.5 3 397.5 4 344.5 5 327.0 6 285.0 7 247.5 8 241.0 9 224.5 10 194.0 11 187.0 12 186.0 13 185.0 14 212.0 15 236.5 16 256.5 17 286.0 18 348.0 19 444.0 20 557.5 21 820.5 22 1200.5 23 1714.5 24 2585.0 25 4069.5 26 5987.0 27 8537.5 28 11439.5 29 14752.5 30 18526.0 31 24273.5 32 35571.5 33 38740.0 34 33977.0 35 34833.0 36 39334.5 37 44300.0 38 47540.0 39 49918.5 40 52230.5 41 55326.5 42 56748.5 43 58759.0 44 61751.0 45 67515.0 46 70711.0 47 70368.5 48 69773.5 49 67021.5 50 67727.0 51 69131.0 52 70752.0 53 76466.0 54 82285.5 55 80388.5 56 74663.0 57 68840.0 58 65041.5 59 58115.0 60 44633.5 61 36126.5 62 32578.5 63 24175.5 64 15561.0 65 12015.0 66 8779.5 67 6523.0 68 5090.5 69 3552.5 70 2440.0 71 1656.5 72 1143.5 73 768.5 74 519.5 75 348.0 76 238.0 77 133.0 78 76.0 79 48.0 80 36.0 81 29.0 82 24.0 83 19.0 84 12.5 85 12.5 86 13.0 87 9.0 88 4.5 89 3.0 90 5.0 91 6.0 92 5.0 93 4.5 94 3.0 95 1.0 96 1.5 97 2.0 98 1.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04259680722425565 2 0.009262388189742567 3 0.002799073354043083 4 0.005394577736883033 5 0.02274883253195015 6 0.028805009425243368 7 0.0038169182100587498 8 2.544612140039167E-4 9 0.0 10-11 1.0178448560156666E-4 12-13 0.0010941832202168416 14-15 4.5803018520705E-4 16-17 2.544612140039167E-4 18-19 1.0178448560156666E-4 20-21 2.5446121400391665E-5 22-23 2.5446121400391665E-5 24-25 0.0 26-27 1.5267672840235E-4 28-29 3.8169182100587497E-4 30-31 3.053534568047E-4 32-33 1.7812284980274164E-4 34-35 8.906142490137083E-4 36-37 1.0178448560156666E-4 38-39 2.29015092603525E-4 40-41 8.142758848125333E-4 42-43 8.142758848125333E-4 44-45 0.001603105648224675 46-47 1.0178448560156666E-4 48-49 0.0 50-51 0.0 52-53 0.0011196293416172332 54-55 0.003104426810847783 56-57 1.5267672840235E-4 58-59 9.160603704141E-4 60-61 0.0011959677058184083 62-63 8.906142490137083E-4 64-65 0.0017303362552266333 66-67 0.0030026423252462165 68-69 0.003587903117455225 70-71 0.0022901509260352497 72-73 0.002799073354043083 74-75 0.002264704804634858 76-77 0.0017048901338262417 78-79 0.0028754117182442584 80-81 0.001450428919822325 82-83 7.633836420117499E-4 84-85 8.142758848125333E-4 86-87 0.002264704804634858 88-89 0.005572700586685775 90-91 6.361530350097916E-4 92-93 0.0014249827984219332 94-95 0.0014249827984219332 96-97 6.615991564101833E-4 98-99 9.923987346152749E-4 100-101 0.0015267672840234999 102-103 0.0018321207408282 104-105 9.669526132148833E-4 106-107 0.0074302674489143666 108-109 0.0023410431688360334 110-111 0.0018830129836289833 112-113 0.002671842747041125 114-115 0.0030026423252462165 116-117 0.0019084591050293749 118-119 0.04447982020788463 120-121 0.012875737428598183 122-123 0.0051910087656799 124-125 0.0030026423252462165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1964936.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.53111672518035 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21660829207023 55.521793713202264 2 10.568947921732589 14.274657115297515 3 3.298251378683218 6.6820379652853035 4 1.4145988273176158 3.821177541075566 5 0.7509575494426785 2.5356500963534456 6 0.40924175723211625 1.6581931725875982 7 0.2979550455204839 1.4084865870530154 8 0.19369576175862732 1.046439287719567 9 0.13523016189996862 0.8219019463228635 >10 0.6562346011845335 7.904475367780542 >50 0.04005930396133485 1.8750756566854203 >100 0.017234566898728855 2.012829177133447 >500 8.206935816232058E-4 0.32477499476934985 >1k 1.6413871632464116E-4 0.11250737873409217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.5446121400391665E-5 0.0 18-19 0.0 0.0 0.0 7.6338364201175E-5 0.0 20-21 0.0 0.0 0.0 1.2723060700195834E-4 0.0 22-23 0.0 0.0 0.0 3.3079957820509166E-4 0.0 24-25 0.0 0.0 0.0 9.415064918144916E-4 0.0 26-27 0.0 0.0 0.0 0.0018830129836289833 0.0 28-29 0.0 0.0 0.0 0.00473297858047285 0.0 30-31 0.0 0.0 0.0 0.015725703025442052 0.0 32-33 0.0 0.0 0.0 0.04162985461104077 0.0 34-35 0.0 0.0 0.0 0.07839950003460673 0.0 36-37 0.0 0.0 0.0 0.13807065471852517 0.0 38-39 0.0 0.0 0.0 0.2314324741365622 0.0 40-41 0.0 0.0 0.0 0.3318428691825077 0.0 42-43 0.0 0.0 0.0 0.43235504871405483 0.0 44-45 0.0 0.0 0.0 0.5568629207261713 0.0 46-47 0.0 0.0 0.0 0.7072240520810855 0.0 48-49 0.0 0.0 0.0 0.8629543150514826 0.0 50-51 0.0 0.0 0.0 1.0442070377864723 0.0 52-53 0.0 0.0 0.0 1.2044667103661393 0.0 54-55 0.0 0.0 0.0 1.3842690041813066 0.0 56-57 0.0 0.0 0.0 1.5888049279976548 0.0 58-59 0.0 0.0 0.0 1.8128834730495038 0.0 60-61 0.0 0.0 0.0 2.063273307629358 0.0 62-63 0.0 0.0 0.0 2.3101261313345574 0.0 64-65 0.0 0.0 0.0 2.549319672498239 0.0 66-67 0.0 0.0 0.0 2.8375478896004758 0.0 68-69 0.0 0.0 0.0 3.1673805151923524 0.0 70-71 0.0 0.0 0.0 3.514949087400302 0.0 72-73 0.0 0.0 0.0 3.8783451471192953 0.0 74-75 0.0 0.0 0.0 4.234463616117777 0.0 76-77 0.0 0.0 0.0 4.624323642093177 0.0 78-79 0.0 0.0 0.0 5.062124160786917 0.0 80-81 0.0 0.0 0.0 5.532699283844359 0.0 82-83 0.0 0.0 0.0 6.026430377376158 0.0 84-85 0.0 0.0 0.0 6.527617184478273 0.0 86-87 0.0 0.0 0.0 7.029847282557803 0.0 88-89 0.0 0.0 0.0 7.556200303724905 0.0 90-91 0.0 0.0 0.0 8.140901281263105 0.0 92-93 0.0 0.0 0.0 8.72343933848227 0.0 94-95 0.0 0.0 0.0 9.32119417629887 0.0 96-97 0.0 0.0 0.0 9.935667115875528 0.0 98-99 0.0 0.0 0.0 10.55164137661481 0.0 100-101 0.0 0.0 0.0 11.242503572635446 0.0 102-103 0.0 0.0 0.0 11.954765956753807 0.0 104-105 0.0 0.0 0.0 12.670641690111026 0.0 106-107 0.0 0.0 0.0 13.382700505258185 0.0 108-109 0.0 0.0 0.0 14.127152232948045 0.0 110-111 0.0 0.0 0.0 14.919977037420047 0.0 112-113 0.0 0.0 0.0 15.749495148951416 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1610 0.0 57.68495 1 GTATCAA 2515 0.0 47.106293 1 TCAACGC 2805 0.0 40.312603 4 ATCAACG 2820 0.0 39.887127 3 CAACGCA 2880 0.0 39.260796 5 AACGCAG 3050 0.0 37.06777 6 GTACATG 7825 0.0 36.7474 1 AGGTAAG 1435 0.0 35.667004 2 TACATGG 8145 0.0 34.999718 2 ACATGGG 8170 0.0 34.01848 3 TATCAAC 3330 0.0 33.95701 2 TAAGGTA 905 0.0 33.538345 4 TAAGGTG 1685 0.0 33.19907 5 GTGTAAG 965 0.0 32.080402 1 GGTAAGG 1820 0.0 29.757065 3 ACGCAGA 3805 0.0 29.23831 7 GTAAGGT 1940 0.0 29.143517 4 CGCAGAG 4040 0.0 27.684124 8 CATGGGA 4665 0.0 27.683958 4 CATGGGG 4420 0.0 27.602707 4 >>END_MODULE