FastQCFastQC Report
Thu 26 May 2016
SRR1364369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964936
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.18249958268360902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.1652471123741435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.071.888231
GTATCAA53400.057.7798271
ATCAACG69150.044.3430753
TCAACGC70100.043.742134
CAACGCA70350.043.671325
TATCAAC70650.043.4858782
AACGCAG73400.041.9153446
TAAGGTG16750.038.3901375
AGGTAAG14850.038.089732
ACGCAGA86400.035.6022957
CGCAGAG88150.034.89558
GTACATG80700.034.3215681
TACATGG81100.033.6244622
GTAAGGT17450.033.0968784
GGTAAGG17900.032.9300883
GTGGTAT12300.032.4457171
ACATGGG82400.032.2268873
GCAGAGT105250.028.6607179
TAAGGTA8150.027.7611054
CATGGGA47050.027.0810094
Produced by FastQC (version 0.11.5)