FastQCFastQC Report
Thu 26 May 2016
SRR1364368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806270
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24850.049.3514181
GGTATCA18450.042.592821
TCAACGC28150.042.0751084
ATCAACG28250.041.50483
CAACGCA29900.039.21445
GTACATG78950.037.7784731
AACGCAG31700.037.5457846
TATCAAC32000.037.3849332
TACATGG85150.034.250112
ACATGGG82900.033.959183
ACGCAGA39650.029.7101087
CATGGGG40000.029.0151754
TAAGGTG9050.028.9370775
CGCAGAG40900.028.655848
CATGGGA52000.027.1266124
ATGGGAG23650.024.9146395
GAGTACA63250.024.2838751
GAGCGCG1250.001634912123.7974389
AGGTAAG8600.022.1463452
ATGGGGA23950.022.117455
Produced by FastQC (version 0.11.5)