##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364368_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1806270 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18361263819916 31.0 31.0 34.0 28.0 34.0 2 31.244428020174173 33.0 31.0 34.0 28.0 34.0 3 31.31423375242904 34.0 31.0 34.0 28.0 34.0 4 34.759553112214675 37.0 35.0 37.0 32.0 37.0 5 34.8208833673814 37.0 35.0 37.0 32.0 37.0 6 34.82190259485016 37.0 35.0 37.0 32.0 37.0 7 34.8102110980086 37.0 35.0 37.0 32.0 37.0 8 34.75064359148964 37.0 35.0 37.0 32.0 37.0 9 36.30524118764083 39.0 37.0 39.0 32.0 39.0 10-11 36.22990527440527 39.0 37.0 39.0 32.0 39.0 12-13 36.245042269428154 39.0 37.0 39.0 32.0 39.0 14-15 37.56921196720313 40.0 38.0 41.0 32.0 41.0 16-17 37.5735933719765 40.0 38.0 41.0 32.0 41.0 18-19 37.56617117042303 40.0 38.0 41.0 32.0 41.0 20-21 37.59596267446174 40.0 38.0 41.0 32.0 41.0 22-23 37.55436784090972 40.0 38.0 41.0 31.5 41.0 24-25 37.553790131043534 40.0 38.0 41.0 31.5 41.0 26-27 37.51183959208756 40.0 38.0 41.0 31.0 41.0 28-29 37.516547360029236 40.0 38.0 41.0 31.0 41.0 30-31 37.42080309145366 40.0 38.0 41.0 31.0 41.0 32-33 37.379349709622595 40.0 38.0 41.0 31.0 41.0 34-35 37.37654282028711 40.0 38.0 41.0 31.0 41.0 36-37 37.31079655865402 40.0 38.0 41.0 31.0 41.0 38-39 37.218237583528484 40.0 37.0 41.0 30.0 41.0 40-41 37.157755761873915 40.0 37.0 41.0 30.0 41.0 42-43 37.015605640352774 40.0 37.0 41.0 30.0 41.0 44-45 36.88709356851412 40.0 37.0 41.0 30.0 41.0 46-47 36.76807177221567 40.0 36.5 41.0 30.0 41.0 48-49 36.62046399486234 40.0 36.0 41.0 30.0 41.0 50-51 36.49338498674063 40.0 36.0 41.0 30.0 41.0 52-53 36.31963272378991 39.5 35.5 41.0 29.5 41.0 54-55 36.1292079810881 39.0 35.0 41.0 29.0 41.0 56-57 35.92954015734082 39.0 35.0 41.0 29.0 41.0 58-59 35.70214115276232 39.0 35.0 41.0 28.5 41.0 60-61 35.44889523714616 38.0 35.0 40.0 28.0 41.0 62-63 35.18031551207737 38.0 34.0 40.0 28.0 41.0 64-65 34.833355201603304 37.0 34.0 40.0 28.0 41.0 66-67 34.51930442292681 37.0 34.0 40.0 27.5 41.0 68-69 34.19884651796244 36.0 34.0 39.0 27.0 41.0 70-71 33.83337097997531 36.0 33.5 39.0 26.0 41.0 72-73 33.46639289807172 35.0 33.0 38.5 26.0 40.0 74-75 33.108305236758625 35.0 33.0 37.5 26.0 39.5 76-77 32.87903109723352 35.0 33.0 37.0 26.0 39.0 78-79 32.7190265021287 35.0 33.0 36.5 26.0 39.0 80-81 32.4511019947184 35.0 33.0 36.0 26.0 38.0 82-83 32.16488398744374 35.0 33.0 36.0 26.0 37.0 84-85 31.93039910976764 35.0 33.0 35.5 25.0 37.0 86-87 31.724248036007907 35.0 33.0 35.0 25.0 36.5 88-89 31.469999224922077 35.0 32.5 35.0 24.0 36.0 90-91 31.34609388408156 35.0 32.0 35.0 24.0 36.0 92-93 31.200841513173557 35.0 32.0 35.0 24.0 36.0 94-95 31.09813759847642 35.0 32.0 35.0 23.0 35.0 96-97 30.9990189728003 35.0 32.0 35.0 23.0 35.0 98-99 30.8614523299396 35.0 32.0 35.0 20.0 35.0 100-101 30.763413000271278 35.0 32.0 35.0 20.0 35.0 102-103 29.72254452545854 33.5 30.0 34.5 18.5 35.0 104-105 30.43497373039468 34.5 31.5 35.0 19.0 35.0 106-107 30.77058856095711 35.0 32.0 35.0 20.0 35.0 108-109 30.79835102171879 35.0 33.0 35.0 19.5 35.0 110-111 30.723482923372472 35.0 32.5 35.0 19.0 35.0 112-113 30.61983424404989 35.0 32.0 35.0 18.0 35.0 114-115 30.50462804564102 35.0 32.0 35.0 16.0 35.0 116-117 30.35999629069851 35.0 32.0 35.0 7.5 35.0 118-119 30.170752434575117 35.0 32.0 35.0 2.0 35.0 120-121 29.987763180476893 35.0 32.0 35.0 2.0 35.0 122-123 29.79016343071634 35.0 32.0 35.0 2.0 35.0 124-125 28.95076705033024 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17205088366439725 1101 2 0.16442221047171657 1101 3 0.23472621062208532 1101 4 0.2458300667832347 1101 5 0.19325942672727336 1101 6 0.12224227622486694 1101 7 0.20716068471288906 1101 8 0.0545935984128576 1101 9 0.1946333532695803 1101 10-11 0.3104299807531845 1101 12-13 0.2792027740542977 1101 14-15 0.42229418455273304 1101 16-17 0.39637331303327983 1101 18-19 0.15650462782581798 1101 20-21 0.31992378306746616 1101 22-23 0.414413591002031 1101 24-25 0.3883101797157238 1101 26-27 0.5164910538263285 1101 28-29 0.4563288788452695 1101 30-31 0.5382524267090218 1101 32-33 0.4155593304540659 1101 34-35 0.4136133480060096 1101 36-37 0.3448142538195995 1101 38-39 0.4600773723132505 1101 40-41 0.3775516397654215 1101 42-43 0.423999103184137 1101 44-45 0.3836510104112918 1101 46-47 0.3730739785177519 1101 48-49 0.32498108522320734 1101 50-51 0.23134948797014232 1101 52-53 0.1514033102872432 1101 54-55 0.27125130361661576 1101 56-57 0.12112548775515819 1101 58-59 0.2234698965744144 1101 60-61 0.21545941344790975 1101 62-63 0.3325931472988657 1101 64-65 0.39304675682468826 1101 66-67 0.32087990446932935 1101 68-69 0.32717599988753676 1101 70-71 0.18693733964837378 1101 72-73 0.22895689388223417 1101 74-75 0.1589536474017308 1101 76-77 0.13608269990403699 1101 78-79 0.17144491082530067 1101 80-81 0.06537935733319244 1101 82-83 -0.07776065388302555 1101 84-85 0.005812347812607754 1101 86-87 -0.031067272477734775 1101 88-89 0.059648307460854255 1101 90-91 0.08315110156251038 1101 92-93 0.022701679709768996 1101 94-95 -0.06452677470565504 1101 96-97 -0.11323240218391817 1101 98-99 -0.09449410137069947 1101 100-101 -0.15677179507300565 1101 102-103 -0.2467309579979471 1101 104-105 -0.11403943698893215 1101 106-107 -0.13763407546749207 1101 108-109 -0.18050326777703773 1101 110-111 -0.22006336506689905 1101 112-113 -0.18898707327674558 1101 114-115 -0.332317498007459 1101 116-117 -0.37933229521150835 1101 118-119 -0.4097515023573095 1101 120-121 -0.4781033479131338 1101 122-123 -0.5306368477434127 1101 124-125 -0.6271770739186273 1102 1 0.03019355300056148 1102 2 0.12061597615099018 1102 3 0.08349614139205741 1102 4 0.0034272782845903293 1102 5 0.02335941225409499 1102 6 0.09258673782270677 1102 7 0.07353542540180769 1102 8 0.09822057641363102 1102 9 0.014650510014078577 1102 10-11 0.08832175154631727 1102 12-13 0.08519851351299934 1102 14-15 0.09409998467816649 1102 16-17 0.22077478688507313 1102 18-19 0.18952517973627891 1102 20-21 0.2623251393247017 1102 22-23 0.2500814527108375 1102 24-25 0.183224742746404 1102 26-27 0.21003473568178777 1102 28-29 0.19664104716830622 1102 30-31 0.19996137716251638 1102 32-33 0.22818039254398315 1102 34-35 0.21564878919667763 1102 36-37 -0.007116030698931297 1102 38-39 0.07477533521367974 1102 40-41 -0.011045532537742986 1102 42-43 -0.018441752783637355 1102 44-45 -0.005071068419276514 1102 46-47 -0.07018091824025419 1102 48-49 0.015100504004571746 1102 50-51 0.021408978755552255 1102 52-53 0.017756642736109995 1102 54-55 0.02709891916081375 1102 56-57 0.05885856619977403 1102 58-59 9.041039267856377E-4 1102 60-61 -0.0034849190974384214 1102 62-63 -0.08616702915328034 1102 64-65 -0.10325331131984683 1102 66-67 -0.038670356650889914 1102 68-69 -0.07341973715896444 1102 70-71 -0.10992529784680016 1102 72-73 -0.10353523632811346 1102 74-75 -0.15999302195855591 1102 76-77 -0.08312192367276339 1102 78-79 -0.004684223075699379 1102 80-81 -0.1421051593833198 1102 82-83 -0.1032225894291443 1102 84-85 -0.059439515608847415 1102 86-87 -0.05992784151478503 1102 88-89 -0.07193231416206913 1102 90-91 -0.18739183854266273 1102 92-93 -0.21076083174816063 1102 94-95 -0.2961482528994033 1102 96-97 -0.28635239687708847 1102 98-99 -0.2156663985050109 1102 100-101 -0.34261619938119026 1102 102-103 -0.4613115524836715 1102 104-105 -0.3109779832789741 1102 106-107 -0.33277968165235805 1102 108-109 -0.34072630545554006 1102 110-111 -0.30754406749839447 1102 112-113 -0.5376921762775169 1102 114-115 -0.4528045053164149 1102 116-117 -0.4539564449123965 1102 118-119 -0.4522508540796153 1102 120-121 -0.566306879971215 1102 122-123 -0.5998159171181747 1102 124-125 -0.6698522898101729 1103 1 0.3773938670871644 1103 2 0.47092209668290863 1103 3 0.3971661720536659 1103 4 0.42188051714736474 1103 5 0.3937523119323316 1103 6 0.3479369868419653 1103 7 0.29035991693788077 1103 8 0.34089582148683206 1103 9 0.45648820067280127 1103 10-11 0.5030617062706213 1103 12-13 0.45584829496748114 1103 14-15 0.5753968759574875 1103 16-17 0.47917832692749585 1103 18-19 0.49588513759967157 1103 20-21 0.5162767769108925 1103 22-23 0.5273113278025363 1103 24-25 0.5490942894081314 1103 26-27 0.5735220792431548 1103 28-29 0.5458056599395604 1103 30-31 0.5599008971478838 1103 32-33 0.542008191368204 1103 34-35 0.5295657902731676 1103 36-37 0.4760755578393372 1103 38-39 0.4611213846264022 1103 40-41 0.4845953915620882 1103 42-43 0.4728702732924219 1103 44-45 0.35585929184096443 1103 46-47 0.36362531523679564 1103 48-49 0.45853703038898885 1103 50-51 0.39881771693718093 1103 52-53 0.4425339218721476 1103 54-55 0.36937513578411796 1103 56-57 0.31910214308287266 1103 58-59 0.3829583859381529 1103 60-61 0.3615176793064734 1103 62-63 0.4002884298544416 1103 64-65 0.40914165968498395 1103 66-67 0.3161550488014484 1103 68-69 0.2548984719793381 1103 70-71 0.3712449646962881 1103 72-73 0.3458430032632194 1103 74-75 0.21746512509912463 1103 76-77 0.2787402524864433 1103 78-79 0.22889890121913226 1103 80-81 0.2878718861996177 1103 82-83 0.35179928287436013 1103 84-85 0.22236038465622698 1103 86-87 0.21078649692625007 1103 88-89 0.34460677454594446 1103 90-91 0.3064617120694102 1103 92-93 0.23217302589125666 1103 94-95 0.2889156361797376 1103 96-97 0.3066090720399224 1103 98-99 0.3064454049056522 1103 100-101 0.31250799663358464 1103 102-103 0.06114330237479848 1103 104-105 0.1915142803117611 1103 106-107 0.20115608000395824 1103 108-109 0.2130073265598753 1103 110-111 0.21330670009216135 1103 112-113 0.16079777604291934 1103 114-115 0.11334318077272343 1103 116-117 0.09611612252517077 1103 118-119 0.12124943490313456 1103 120-121 0.056340299676939765 1103 122-123 -0.008336936378906756 1103 124-125 -0.08241270658439959 1104 1 0.26662275523159096 1104 2 0.26237954638347105 1104 3 0.28950768375463554 1104 4 0.3802577031801633 1104 5 0.29187543276901806 1104 6 0.18830324290374278 1104 7 0.3064845644730454 1104 8 0.3525751070177634 1104 9 0.39708337729923926 1104 10-11 0.43247561994212447 1104 12-13 0.3375387058329551 1104 14-15 0.38716525398700696 1104 16-17 0.2898678548677722 1104 18-19 0.3645534194772324 1104 20-21 0.3806044660819268 1104 22-23 0.42299296611646753 1104 24-25 0.3156042255093894 1104 26-27 0.3988826772014704 1104 28-29 0.3322215520876739 1104 30-31 0.3560602638108108 1104 32-33 0.3456534752270386 1104 34-35 0.3755276250565487 1104 36-37 0.32887322088752313 1104 38-39 0.10906798358202252 1104 40-41 0.1677413167381374 1104 42-43 0.17500856343221471 1104 44-45 0.13735586897958996 1104 46-47 0.18369566013620897 1104 48-49 0.08177582902127511 1104 50-51 3.4049973323391214E-4 1104 52-53 -0.043410431245156644 1104 54-55 0.02921069921347197 1104 56-57 -0.03490910351177945 1104 58-59 -0.04242257642082592 1104 60-61 -0.20953786891202952 1104 62-63 -0.09300780873830661 1104 64-65 0.05501974920385777 1104 66-67 0.059818751846663076 1104 68-69 0.033612873399995635 1104 70-71 -0.10827246216414466 1104 72-73 -0.10243885744511516 1104 74-75 -0.10662708367205198 1104 76-77 -0.14368625778087107 1104 78-79 0.03865708413636071 1104 80-81 -0.006126973337799768 1104 82-83 -0.031407440774266604 1104 84-85 -0.020132961826718088 1104 86-87 -0.04686548627304887 1104 88-89 0.03983113311995368 1104 90-91 -0.16890150336700316 1104 92-93 -0.16315879664962551 1104 94-95 -0.227564581369883 1104 96-97 -0.21850777063869842 1104 98-99 -0.3309160564695688 1104 100-101 -0.3753542311083429 1104 102-103 -0.4802126411039538 1104 104-105 -0.4119722426233139 1104 106-107 -0.3856220205883254 1104 108-109 -0.3705740122387766 1104 110-111 -0.43936490296510655 1104 112-113 -0.4388793689102144 1104 114-115 -0.45739981389449724 1104 116-117 -0.47725873584680656 1104 118-119 -0.4840927607104746 1104 120-121 -0.5245006869144149 1104 122-123 -0.5834480318334911 1104 124-125 -0.7397049654876504 1105 1 0.19503891678599672 1105 2 0.20437442803070027 1105 3 0.08002524079449813 1105 4 0.282235466057287 1105 5 0.34076679968553236 1105 6 0.36083120544446956 1105 7 0.30672752341524756 1105 8 0.3325200956905334 1105 9 0.3500758725527007 1105 10-11 0.35249341655173083 1105 12-13 0.41786933201709076 1105 14-15 0.5176811230736007 1105 16-17 0.4051400439770205 1105 18-19 0.3265704172995001 1105 20-21 0.37639674416092817 1105 22-23 0.3709326330846139 1105 24-25 0.3479917955278822 1105 26-27 0.4102265205913014 1105 28-29 0.3566159988543447 1105 30-31 0.2919452862825196 1105 32-33 0.4135480725139544 1105 34-35 0.2656699779969429 1105 36-37 0.2613470493050798 1105 38-39 0.23743317426425392 1105 40-41 0.2828817923289435 1105 42-43 0.33271018247179285 1105 44-45 0.3004429228432386 1105 46-47 0.25803597942518763 1105 48-49 0.19477074323138055 1105 50-51 0.19612447095561691 1105 52-53 0.2511303690440485 1105 54-55 0.2529061073824934 1105 56-57 0.12100780399835287 1105 58-59 0.02560380083939151 1105 60-61 0.04702630886533399 1105 62-63 0.12279881029795092 1105 64-65 0.059551903716709376 1105 66-67 0.05175664845753403 1105 68-69 0.025460723685178266 1105 70-71 0.05571711505672283 1105 72-73 -0.049428286471673744 1105 74-75 -0.0474243159603418 1105 76-77 0.0770341659884366 1105 78-79 0.1670966377989913 1105 80-81 0.09150763262810813 1105 82-83 0.13603812107160707 1105 84-85 0.049683576260889595 1105 86-87 0.18480231123369606 1105 88-89 0.2302068090488767 1105 90-91 0.16516072894454226 1105 92-93 0.06654844978690022 1105 94-95 0.12951814362465086 1105 96-97 0.11939435598305081 1105 98-99 0.041444107567592425 1105 100-101 -0.007570414061213171 1105 102-103 -0.16433850393715232 1105 104-105 0.01903572464900094 1105 106-107 -0.053866635585457345 1105 108-109 -0.10633768814908962 1105 110-111 -0.02507697668214348 1105 112-113 -0.10211873729670984 1105 114-115 -0.10795660587950806 1105 116-117 -0.16488909534762186 1105 118-119 -0.177699338677634 1105 120-121 -0.35106621372528934 1105 122-123 -0.3711072991953195 1105 124-125 -0.4629295410238896 1106 1 0.04831081863549613 1106 2 0.16204007535610998 1106 3 0.06468240643133427 1106 4 0.1254724078237004 1106 5 0.1722605105423014 1106 6 0.006201813797119371 1106 7 0.09978848456837852 1106 8 0.12063406084060802 1106 9 0.22981895659328444 1106 10-11 0.2791235320537595 1106 12-13 0.22332420649649976 1106 14-15 0.20576374741401082 1106 16-17 0.2717932913569854 1106 18-19 0.17807254690096386 1106 20-21 0.042764055827575476 1106 22-23 0.19894777633440697 1106 24-25 0.20701840767814872 1106 26-27 0.3247969154592738 1106 28-29 0.28694439546377026 1106 30-31 0.4473632467957245 1106 32-33 0.3572342762633127 1106 34-35 0.3133762634684416 1106 36-37 0.3104516725334676 1106 38-39 0.26725087303579187 1106 40-41 0.28405340096484366 1106 42-43 0.16994044169569378 1106 44-45 0.23007893671186963 1106 46-47 0.1882022299628403 1106 48-49 0.13116673370586795 1106 50-51 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-0.8979044032304841 2216 94-95 -0.8180252023786139 2216 96-97 -0.8366846427499546 2216 98-99 -0.8693835471254161 2216 100-101 -0.6965381571013083 2216 102-103 -0.7555568719130434 2216 104-105 -0.8894656850235201 2216 106-107 -0.6865162492096282 2216 108-109 -0.6697352842864746 2216 110-111 -0.6805193399096652 2216 112-113 -0.6774771479622785 2216 114-115 -0.7138070322055725 2216 116-117 -0.7287107346769162 2216 118-119 -0.673530246461084 2216 120-121 -0.5802124772370298 2216 122-123 -0.7103096071773791 2216 124-125 -0.5560857806796342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27236.0 3 5085.0 4 3321.0 5 2789.0 6 3056.0 7 3488.0 8 3896.0 9 4478.0 10 4936.0 11 5592.0 12 6136.0 13 6071.0 14 5938.0 15 5710.0 16 5192.0 17 4877.0 18 4618.0 19 4604.0 20 4807.0 21 5166.0 22 6077.0 23 7894.0 24 11382.0 25 15795.0 26 18817.0 27 19992.0 28 23726.0 29 28599.0 30 34355.0 31 42603.0 32 54491.0 33 72645.0 34 102394.0 35 155625.0 36 301915.0 37 562688.0 38 229827.0 39 449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.634866513464964 18.098626224962793 12.55467434115238 24.71183292041986 2 15.613590785922865 19.881668074852747 39.20844070060118 25.296300438623202 3 18.778093783339525 24.37838654831314 29.435668938621777 27.407850729725553 4 12.654241527823684 16.02434312916176 36.746287877655405 34.57512746535915 5 13.491343602284156 38.346368875953026 34.41881636830004 13.74347115346278 6 32.824988882618726 36.2017930688458 17.337576859454703 13.635641189080767 7 28.945502058171368 32.576963429049144 20.716581359361978 17.760953153417507 8 27.038316530751217 34.93990377961213 19.793995360605006 18.227784329031653 9 26.88579226804409 15.963615627785435 19.17526172720579 37.975330376964685 10-11 25.039342179383727 26.494829807877295 27.720478832731033 20.745349180007942 12-13 25.547806531657596 23.869604910507906 27.692001490381447 22.890587067453048 14-15 22.37496553656114 25.63484699340406 24.758977379804257 27.23121009023054 16-17 22.925467614587756 29.006235783882627 25.611225573606582 22.457071027923035 18-19 23.09537590895263 27.847880865995283 26.68642563384126 22.37031759121083 20-21 23.416598847348403 27.13733273541608 26.70702608137211 22.73904233586341 22-23 23.449380062056076 27.059749389556764 26.588058980124508 22.902811568262653 24-25 23.16544591893792 27.328001904477183 26.56200346570557 22.944548710879324 26-27 22.92827842135407 27.50879644482731 26.328656936298817 23.234268197519807 28-29 23.002019088049845 27.560136657991286 26.39513337219815 23.04271088176072 30-31 22.978053632159586 27.078665557196143 26.53970869201508 23.403572118629196 32-33 22.66078823960265 27.47580574395071 26.431662454938103 23.43174356150854 34-35 23.406306704073764 27.333249365744823 26.08837911643029 23.172064813751124 36-37 23.5160644632487 26.969231335634024 26.035760444385513 23.47894375673176 38-39 23.027818415600127 26.985427130809803 26.405114414116404 23.581640039473665 40-41 23.25191922866011 26.782668824717597 26.560496982853238 23.40491496376905 42-43 23.243755726627747 26.822209488693456 26.46190598780349 23.472128796875303 44-45 23.008397829635644 26.94829398410677 26.653012154250778 23.390296032006813 46-47 23.279859400825845 27.307552942681035 26.121185127689973 23.291402528803147 48-49 23.156421797405706 27.208639904333236 26.090036373299675 23.544901924961383 50-51 23.126996517685615 27.00595702746544 26.498170262474602 23.36887619237434 52-53 23.403093867655677 26.89552196663752 26.38759131139341 23.313792854313398 54-55 22.91971170668403 26.978417863644218 26.502201287110733 23.599669142561023 56-57 23.01044944314757 27.273907505999244 26.499327204122753 23.216315846730428 58-59 23.09907258465229 26.770865599653206 26.308998749623875 23.82106306607063 60-61 22.642885160660253 26.824201197010712 26.999232387330764 23.53368125499827 62-63 22.901720378494893 26.915014149406552 26.767803850997048 23.415461621101507 64-65 22.70012440214169 27.076953825047518 26.521600961998935 23.701320810811858 66-67 22.67330150449266 27.221631210091108 26.382919943185325 23.722147342230905 68-69 22.74266286015786 26.686180883726067 26.798349799261377 23.772806456854692 70-71 22.672235912500575 26.65376317177944 26.654953497037198 24.019047418682792 72-73 22.759927671914845 26.722442634150774 26.49522703289865 24.022402661035734 74-75 22.652141392767593 26.928864580444028 26.661761424756858 23.75723260203152 76-77 22.89838230738845 26.691692795912846 26.62295906065925 23.786965836039453 78-79 22.91593885798028 26.68784716820367 26.780305400059017 23.615908573757032 80-81 22.821700010186863 26.886451915794506 26.72393270470057 23.567915369318058 82-83 22.61891975684564 26.93136646994342 26.73145062172666 23.71826315148428 84-85 22.64699507462798 26.729658722902215 26.85267537693227 23.770670825537525 86-87 22.801642869856792 26.773923247294984 26.66322302896725 23.76121085388097 88-89 22.711425515234463 26.845730851172206 26.590023740870173 23.852819892723158 90-91 22.69748615855031 26.923373967029658 26.64365225483935 23.735487619580688 92-93 22.85212694574179 26.338988136760538 26.913686462354093 23.89519845514358 94-95 22.52115714027879 26.385885422029997 26.992032093081452 24.10092534460976 96-97 22.72147220922733 26.22350541033569 26.94217072245767 24.112851657979313 98-99 22.734107569648327 26.254074041676333 26.620163399036567 24.391654989638774 100-101 22.367751590180934 26.226179120062053 27.05558117659825 24.350488113158764 102-103 22.39962862119251 25.949761992025433 27.17110996200952 24.479499424772538 104-105 22.390566332309852 26.09082111375255 27.04926881822822 24.469343735709373 106-107 22.45406768925485 26.233932549929794 26.831894165223442 24.480105595591915 108-109 22.528988652570224 26.0571778472659 26.707540150058907 24.70629335010497 110-111 22.390244915189726 26.396307240880564 26.940947688219296 24.272500155710418 112-113 22.40222630132633 26.412580495946102 27.130791327434657 24.05440187529291 114-115 22.242765573722842 26.4021437182605 26.867344975591124 24.48774573242554 116-117 22.116255533631413 26.431685257896564 26.99039102483186 24.46166818364016 118-119 21.889517760080025 26.639026120786657 26.712997029255202 24.758459089878116 120-121 22.018078885200936 26.93646476981688 26.728550115045184 24.316906229936997 122-123 22.124947956345338 26.97821253299789 26.615235724536255 24.281603786120513 124-125 22.15108193279637 26.895486244139672 26.572988109922868 24.38044371314109 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 500.0 1 446.0 2 356.5 3 282.5 4 232.0 5 208.5 6 185.0 7 165.5 8 162.5 9 173.5 10 155.5 11 141.0 12 156.0 13 157.0 14 161.5 15 183.5 16 223.5 17 287.5 18 370.5 19 441.5 20 567.0 21 801.0 22 1110.5 23 1591.0 24 2373.5 25 3431.0 26 4947.0 27 7068.5 28 9547.5 29 12590.5 30 17059.0 31 21821.5 32 27190.5 33 30196.0 34 31552.5 35 33987.5 36 37291.0 37 41648.5 38 44595.5 39 46870.5 40 49526.0 41 54857.5 42 59914.5 43 64090.5 44 69668.5 45 73600.5 46 74287.5 47 74645.0 48 75234.0 49 72533.5 50 69198.0 51 66659.0 52 65182.5 53 65979.5 54 66473.5 55 63907.5 56 57674.5 57 52677.0 58 50150.5 59 44131.0 60 35097.5 61 27991.5 62 23386.5 63 18032.5 64 13218.5 65 10243.5 66 7551.5 67 6129.5 68 4563.5 69 2700.0 70 1754.0 71 1212.5 72 865.5 73 602.5 74 480.0 75 402.5 76 266.5 77 139.5 78 72.5 79 42.0 80 29.5 81 18.5 82 13.0 83 12.0 84 11.5 85 9.5 86 6.0 87 8.0 88 7.5 89 3.0 90 2.5 91 3.0 92 1.5 93 4.0 94 6.0 95 3.5 96 1.0 97 1.5 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04467770599079872 2 0.009079484241004944 3 0.002380596477824467 4 0.0051487319171552425 5 0.024248866448537593 6 0.029840500035985762 7 0.003044948983263853 8 0.0 9 0.0 10-11 1.3840677196653878E-4 12-13 0.0011626168845189258 14-15 4.429016702929241E-4 16-17 8.304406317992327E-5 18-19 8.304406317992327E-5 20-21 0.0 22-23 2.7681354393307755E-5 24-25 0.0 26-27 2.7681354393307755E-5 28-29 4.982643790795397E-4 30-31 1.6608812635984655E-4 32-33 2.4913218953976984E-4 34-35 9.688474037657714E-4 36-37 2.7681354393307755E-5 38-39 1.3840677196653878E-4 40-41 5.813084422594629E-4 42-43 8.304406317992327E-4 44-45 0.0016331999092051577 46-47 2.7681354393307755E-5 48-49 0.0 50-51 0.0 52-53 0.0010795728213390024 54-55 0.0029895862744772376 56-57 8.304406317992327E-5 58-59 7.473965686193094E-4 60-61 0.001134935530125618 62-63 5.813084422594629E-4 64-65 0.001716243972385081 66-67 0.0025743659585776213 68-69 0.003626257425523316 70-71 0.002269871060251236 72-73 0.0031003116920504687 74-75 0.002159145642678005 76-77 0.00135638636527208 78-79 0.00271277273054416 80-81 0.0012179795933055413 82-83 5.536270878661551E-4 84-85 5.813084422594629E-4 86-87 0.002242189705857928 88-89 0.005646996296234782 90-91 5.813084422594629E-4 92-93 9.965287581590794E-4 94-95 0.0013287050108787724 96-97 2.7681354393307756E-4 98-99 9.411660493724636E-4 100-101 0.0016055185548118497 102-103 0.0015501558460252344 104-105 8.858033405858482E-4 106-107 0.0074186029774064785 108-109 0.0025466846041843137 110-111 0.0017992880355650043 112-113 0.0025190032497910057 114-115 0.0034601692991634695 116-117 0.0023252337690378515 118-119 0.04597872964728419 120-121 0.013314731463181032 122-123 0.0052040946259418585 124-125 0.0032110371096236994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1806270.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.89140298492326 #Duplication Level Percentage of deduplicated Percentage of total 1 84.72104894233509 65.14320315530495 2 9.916118181747885 15.249284783178025 3 2.7826779713098735 6.418920398077688 4 1.0859546613080064 3.3400230994395907 5 0.5219246980018576 2.0065761140922604 6 0.283601129183973 1.308389322663849 7 0.17541482196505664 0.9441524235670619 8 0.11828949591790217 0.727635623960548 9 0.08361524330262898 0.5786364031618366 >10 0.30256116421851126 3.718562457403364 >50 0.007790129904559629 0.3959138765969784 >100 8.781158203360961E-4 0.09655294906533021 >500 1.2544498430599744E-4 0.07214939348850949 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1072541757323102E-4 0.0 0.0 0.0 0.0 3 1.1072541757323102E-4 0.0 0.0 0.0 0.0 4 1.1072541757323102E-4 0.0 0.0 0.0 0.0 5 1.1072541757323102E-4 0.0 0.0 0.0 0.0 6 2.2145083514646204E-4 0.0 0.0 0.0 0.0 7 2.7681354393307756E-4 0.0 0.0 0.0 0.0 8 2.7681354393307756E-4 0.0 0.0 0.0 0.0 9 2.7681354393307756E-4 0.0 0.0 0.0 0.0 10-11 2.7681354393307756E-4 0.0 0.0 0.0 0.0 12-13 3.598576071130008E-4 0.0 0.0 0.0 0.0 14-15 3.875389615063086E-4 0.0 0.0 0.0 0.0 16-17 3.875389615063086E-4 0.0 0.0 0.0 0.0 18-19 4.1522031589961636E-4 0.0 0.0 0.0 0.0 20-21 4.429016702929241E-4 0.0 0.0 8.304406317992326E-5 0.0 22-23 4.429016702929241E-4 0.0 0.0 2.491321895397698E-4 0.0 24-25 7.750779230126172E-4 0.0 0.0 6.920338598326939E-4 0.0 26-27 7.750779230126172E-4 0.0 0.0 0.00160551855481185 0.0 28-29 0.001024210112552387 0.0 0.0 0.004705830246862318 0.0 30-31 0.0011626168845189258 0.0 0.0 0.013453138235147568 0.0 32-33 0.0011626168845189258 0.0 0.0 0.03598576071130008 0.0 34-35 0.0012179795933055413 0.0 0.0 0.06978469442552884 0.0 36-37 0.0012733423020921569 0.0 0.0 0.12824771490419484 0.0 38-39 0.0013840677196653877 0.0 0.0 0.21638514729248673 0.0 40-41 0.0013840677196653877 0.0 0.0 0.3090346404468878 0.0 42-43 0.001467111782845311 0.0 0.0 0.39481915771174847 0.0 44-45 0.0015501558460252344 0.0 0.0 0.5036345618318413 0.0 46-47 0.0015501558460252344 0.0 0.0 0.6414600253561207 0.0 48-49 0.0015501558460252344 0.0 0.0 0.7814723158774712 0.0 50-51 0.0015501558460252344 0.0 0.0 0.9361557242272749 0.0 52-53 0.0015501558460252344 0.0 0.0 1.0832821228277059 0.0 54-55 0.0015501558460252344 0.0 0.0 1.2411488869327398 0.0 56-57 0.0018269693899583119 0.0 0.0 1.429437459516019 0.0 58-59 0.0018269693899583119 0.0 0.0 1.6411721392704302 0.0 60-61 0.0018269693899583119 0.0 0.0 1.8683806961307003 0.0 62-63 0.0018269693899583119 0.0 0.0 2.089250222834903 0.0 64-65 0.0018823320987449274 0.0 0.0 2.3088464072370134 0.0 66-67 0.002159145642678005 0.0 0.0 2.5709611519872446 0.0 68-69 0.002159145642678005 0.0 0.0 2.8761757655278557 0.0 70-71 0.002186826997071313 0.0 0.0 3.2053070692642853 0.0 72-73 0.0022145083514646204 0.0 0.0 3.5381753558438107 0.0 74-75 0.002269871060251236 0.0 0.0 3.863901852989863 5.536270878661551E-5 76-77 0.002269871060251236 0.0 0.0 4.215538097848052 5.536270878661551E-5 78-79 0.002269871060251236 0.0 0.0 4.614841634971516 5.536270878661551E-5 80-81 0.0025466846041843137 0.0 0.0 5.05782634932762 5.536270878661551E-5 82-83 0.0025466846041843137 0.0 0.0 5.510305768240629 5.536270878661551E-5 84-85 0.0026574100217575444 0.0 0.0 5.948390882869117 5.536270878661551E-5 86-87 0.0027681354393307754 0.0 0.0 6.408371948822712 5.536270878661551E-5 88-89 0.0027681354393307754 0.0 0.0 6.898359602938653 5.536270878661551E-5 90-91 0.002934223565690622 0.0 0.0 7.447640718165058 5.536270878661551E-5 92-93 0.0029895862744772376 0.0 0.0 8.002458104270126 5.536270878661551E-5 94-95 0.0029895862744772376 0.0 0.0 8.559932900396952 5.536270878661551E-5 96-97 0.0029895862744772376 0.0 0.0 9.12305469282001 5.536270878661551E-5 98-99 0.003044948983263853 0.0 0.0 9.699961799730938 5.536270878661551E-5 100-101 0.0031556744008370843 0.0 0.0 10.347484041699193 5.536270878661551E-5 102-103 0.0032110371096237 0.0 0.0 11.026230851423097 5.536270878661551E-5 104-105 0.003875389615063086 0.0 0.0 11.703621274781732 5.536270878661551E-5 106-107 0.004041477741422932 0.0 0.0 12.371683081709822 5.536270878661551E-5 108-109 0.004124521804602856 0.0 0.0 13.057239504614483 5.536270878661551E-5 110-111 0.004290609930962702 0.0 0.0 13.79818631766015 5.536270878661551E-5 112-113 0.004373653994142626 0.0 0.0 14.578966599677788 5.536270878661551E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2485 0.0 49.351418 1 GGTATCA 1845 0.0 42.59282 1 TCAACGC 2815 0.0 42.075108 4 ATCAACG 2825 0.0 41.5048 3 CAACGCA 2990 0.0 39.2144 5 GTACATG 7895 0.0 37.778473 1 AACGCAG 3170 0.0 37.545784 6 TATCAAC 3200 0.0 37.384933 2 TACATGG 8515 0.0 34.25011 2 ACATGGG 8290 0.0 33.95918 3 ACGCAGA 3965 0.0 29.710108 7 CATGGGG 4000 0.0 29.015175 4 TAAGGTG 905 0.0 28.937077 5 CGCAGAG 4090 0.0 28.65584 8 CATGGGA 5200 0.0 27.126612 4 ATGGGAG 2365 0.0 24.914639 5 GAGTACA 6325 0.0 24.283875 1 GAGCGCG 125 0.0016349121 23.797438 9 AGGTAAG 860 0.0 22.146345 2 ATGGGGA 2395 0.0 22.11745 5 >>END_MODULE