FastQCFastQC Report
Thu 26 May 2016
SRR1364368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806270
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.1630985400853693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.156953279410055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.062.9610441
GTATCAA47250.054.6999131
ATCAACG58750.043.6801263
TCAACGC59900.042.940934
TATCAAC61100.042.0975722
CAACGCA61250.041.8972665
AACGCAG63600.040.326836
TAACGCA1053.2476237E-739.693854
GTACATG77000.036.8915021
ACGCAGA74500.034.3410847
CGCAGAG75700.033.718118
TACATGG82600.033.6628842
ACATGGG80650.033.2955863
GTGGTAT15150.030.2675171
GTAAGGT9050.029.6059114
TAAGGTG9350.029.292795
GCAGAGT88100.028.4995739
CATGGGG40350.027.7414264
CATGGGA50100.026.8587114
TGGTATC16050.026.7098792
Produced by FastQC (version 0.11.5)