FastQCFastQC Report
Thu 26 May 2016
SRR1364367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799772
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.059.0208361
GTATCAA30300.053.8401531
TCAACGC38000.042.2907834
ATCAACG38550.041.8418123
CAACGCA39650.040.6821335
TATCAAC39650.040.6812
AACGCAG42700.037.4943476
GTACATG92150.036.4403151
TACATGG92700.035.6351662
ACATGGG95000.033.707323
ACGCAGA50050.031.7380057
CGCAGAG50750.031.301988
CATGGGA62500.030.1887254
AGGTAAG11600.029.2470742
TAAGGTA7700.028.6006934
CATGGGG37400.027.5321454
TAAGGTG14950.027.4716995
GTAAGGT14350.026.5456664
ATGGGAT24100.025.9328295
GGTAAGG14000.025.5087283
Produced by FastQC (version 0.11.5)