##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1799772 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39718697701709 33.0 31.0 34.0 28.0 34.0 2 31.379106909097374 33.0 31.0 34.0 28.0 34.0 3 31.524835368035507 33.0 31.0 34.0 28.0 34.0 4 34.9765442511607 37.0 35.0 37.0 32.0 37.0 5 35.01327223670554 37.0 35.0 37.0 32.0 37.0 6 35.08367170952765 37.0 35.0 37.0 32.0 37.0 7 34.9949365808558 37.0 35.0 37.0 32.0 37.0 8 34.889858826562474 37.0 35.0 37.0 32.0 37.0 9 36.471436381941714 39.0 37.0 39.0 32.0 39.0 10-11 36.42316415634869 39.0 37.0 39.0 32.0 39.0 12-13 36.45137245162165 39.0 37.0 39.0 32.0 39.0 14-15 37.80931473542205 40.0 38.0 41.0 32.0 41.0 16-17 37.81485154786273 40.0 38.0 41.0 32.0 41.0 18-19 37.82568819828289 40.0 38.0 41.0 32.0 41.0 20-21 37.859311901729775 40.0 38.0 41.0 32.0 41.0 22-23 37.852026534472145 40.0 38.0 41.0 32.0 41.0 24-25 37.86982017722245 40.0 38.0 41.0 32.0 41.0 26-27 37.839738589110176 40.0 38.0 41.0 32.0 41.0 28-29 37.84909810798257 40.0 38.0 41.0 32.0 41.0 30-31 37.76218098736951 40.0 38.0 41.0 32.0 41.0 32-33 37.72726406455929 40.0 38.0 41.0 32.0 41.0 34-35 37.701675823382075 40.0 38.0 41.0 32.0 41.0 36-37 37.631745576661935 40.0 38.0 41.0 32.0 41.0 38-39 37.5300549180674 40.0 38.0 41.0 31.0 41.0 40-41 37.46833237765672 40.0 38.0 41.0 31.0 41.0 42-43 37.322485848207435 40.0 37.0 41.0 31.0 41.0 44-45 37.17364810653794 40.0 37.0 41.0 31.0 41.0 46-47 37.05295920816637 40.0 37.0 41.0 30.0 41.0 48-49 36.90000400050673 40.0 36.5 41.0 30.0 41.0 50-51 36.763869812398454 40.0 36.0 41.0 30.0 41.0 52-53 36.58630593208473 40.0 36.0 41.0 30.0 41.0 54-55 36.39364763981216 39.0 35.0 41.0 30.0 41.0 56-57 36.190729436839774 39.0 35.0 41.0 30.0 41.0 58-59 35.9629775327097 39.0 35.0 41.0 29.5 41.0 60-61 35.704672036235706 38.0 35.0 40.0 29.0 41.0 62-63 35.41993124684682 38.0 34.5 40.0 29.0 41.0 64-65 35.05240663817417 37.0 34.0 40.0 28.0 41.0 66-67 34.73536064568179 37.0 34.0 40.0 28.0 41.0 68-69 34.396220187890464 36.0 34.0 39.0 28.0 41.0 70-71 34.02823941032531 36.0 34.0 39.0 28.0 41.0 72-73 33.67594534196554 35.0 33.5 38.5 27.0 40.0 74-75 33.31368001057912 35.0 33.0 37.0 26.5 39.5 76-77 33.09405663606279 35.0 33.0 37.0 26.5 39.0 78-79 32.940751939690145 35.0 33.0 36.5 27.0 39.0 80-81 32.690054906954884 35.0 33.0 36.0 27.0 38.5 82-83 32.40657038780468 35.0 33.0 36.0 26.0 37.0 84-85 32.16294841791071 35.0 33.0 35.0 26.0 37.0 86-87 31.974969051635426 35.0 33.0 35.0 26.0 36.5 88-89 31.7195997604141 35.0 33.0 35.0 25.0 36.0 90-91 31.597007009776792 35.0 33.0 35.0 25.0 36.0 92-93 31.45893035340032 35.0 33.0 35.0 25.0 36.0 94-95 31.353859544431185 35.0 33.0 35.0 25.0 35.0 96-97 31.25048339456331 35.0 32.5 35.0 24.0 35.0 98-99 31.121927944206266 35.0 32.0 35.0 24.0 35.0 100-101 31.025004000506733 35.0 32.0 35.0 23.0 35.0 102-103 29.988805248664832 33.5 30.0 34.5 19.5 35.0 104-105 30.710004656145337 34.5 31.5 35.0 20.0 35.0 106-107 31.066558986360494 35.0 32.5 35.0 23.5 35.0 108-109 31.12548561706705 35.0 33.0 35.0 23.5 35.0 110-111 31.04890147196423 35.0 33.0 35.0 23.0 35.0 112-113 30.951373007247582 35.0 33.0 35.0 21.5 35.0 114-115 30.849006707516285 35.0 33.0 35.0 20.0 35.0 116-117 30.709085650849108 35.0 33.0 35.0 19.0 35.0 118-119 30.52578909995266 35.0 32.0 35.0 16.5 35.0 120-121 30.341641885749972 35.0 32.0 35.0 4.5 35.0 122-123 30.149256127998434 35.0 32.0 35.0 2.0 35.0 124-125 29.272194755780177 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03361325393997916 1101 2 0.07263376684189282 1101 3 0.05259385031515862 1101 4 0.09822207108726388 1101 5 0.03728263876229221 1101 6 0.059242257324378045 1101 7 0.12894913223750137 1101 8 0.0038005893931440937 1101 9 0.16415783447940413 1101 10-11 0.23556334098862663 1101 12-13 0.30829255417005896 1101 14-15 0.3901577494954793 1101 16-17 0.28310913943143134 1101 18-19 0.21494409720484242 1101 20-21 0.27688102665544534 1101 22-23 0.33198902638282135 1101 24-25 0.34651337428476126 1101 26-27 0.4244279645563225 1101 28-29 0.329240178815283 1101 30-31 0.33253510954106247 1101 32-33 0.2135411028883638 1101 34-35 0.2628493328369359 1101 36-37 0.2136905093417809 1101 38-39 0.2740434095180433 1101 40-41 0.21036455441556967 1101 42-43 0.33951893936426103 1101 44-45 0.33975973636592016 1101 46-47 0.3300339414058726 1101 48-49 0.26529356482063804 1101 50-51 0.14901434387183343 1101 52-53 0.1182296241057017 1101 54-55 0.12366093339791462 1101 56-57 0.10494446352377906 1101 58-59 0.09783727427718958 1101 60-61 0.08602036620609255 1101 62-63 0.12232445789860691 1101 64-65 0.16500088379143563 1101 66-67 0.059303559397712036 1101 68-69 0.09407844922110797 1101 70-71 0.12287078111914695 1101 72-73 0.10254557951004983 1101 74-75 0.11916812540999899 1101 76-77 0.22493500027745483 1101 78-79 0.1708767795734829 1101 80-81 0.22994300496976194 1101 82-83 0.11740519519660353 1101 84-85 0.0891358414471668 1101 86-87 0.11123310484159177 1101 88-89 0.20001714296477502 1101 90-91 0.14487459778051104 1101 92-93 0.0695187441028402 1101 94-95 -0.021044873129891783 1101 96-97 -0.00164569312557461 1101 98-99 -0.06645550645384901 1101 100-101 -0.18127423412979482 1101 102-103 -0.24739048474344116 1101 104-105 -0.2393455290292934 1101 106-107 -0.09739795827051978 1101 108-109 -0.17036093882704506 1101 110-111 -0.10751674095013897 1101 112-113 -0.035697230938410485 1101 114-115 -0.12583559093024732 1101 116-117 -0.2544012324691316 1101 118-119 -0.21316080030839757 1101 120-121 -0.352670807088149 1101 122-123 -0.31028038534975977 1101 124-125 -0.31772956610752345 1102 1 0.014623965724837262 1102 2 0.11767270588313394 1102 3 0.131023135502236 1102 4 0.1796131837124122 1102 5 0.10672944888360547 1102 6 0.19016236368521078 1102 7 0.1052923635628531 1102 8 0.15998021050633326 1102 9 0.2265028826445814 1102 10-11 0.14180624395802255 1102 12-13 0.15564902855450669 1102 14-15 0.19082463380441794 1102 16-17 0.30160695827735395 1102 18-19 0.32656974303461794 1102 20-21 0.36374335845582095 1102 22-23 0.3199891304726066 1102 24-25 0.23275684501660265 1102 26-27 0.20942223961829853 1102 28-29 0.10978520710877149 1102 30-31 0.2371794063672681 1102 32-33 0.2492734691329943 1102 34-35 0.17091853834801896 1102 36-37 0.09196578896478513 1102 38-39 0.16640511204837338 1102 40-41 0.2039949694499228 1102 42-43 0.14915587893541726 1102 44-45 0.19358456272524194 1102 46-47 0.0027307184077187685 1102 48-49 0.06339960392054422 1102 50-51 -0.05763226185500514 1102 52-53 -0.11237123416186279 1102 54-55 -0.017141196657540547 1102 56-57 -0.002013701846493632 1102 58-59 -0.0010450616867743179 1102 60-61 0.01585971309002332 1102 62-63 -0.04226928441294575 1102 64-65 -0.0709499527119064 1102 66-67 -0.05699252398766674 1102 68-69 -0.1156861186170417 1102 70-71 -0.16559926468035258 1102 72-73 -0.06544231768709352 1102 74-75 -0.09050418103032598 1102 76-77 -0.03744340393155454 1102 78-79 0.09624381433947349 1102 80-81 0.015323476780352507 1102 82-83 -0.08805384074275935 1102 84-85 -0.08435698582057682 1102 86-87 -0.10948572973135029 1102 88-89 -0.018119555307333002 1102 90-91 -0.15819640564498982 1102 92-93 -0.26414755824958647 1102 94-95 -0.31447515190125586 1102 96-97 -0.30802982700395987 1102 98-99 -0.3655811522836352 1102 100-101 -0.44467165364946837 1102 102-103 -0.6242333470231927 1102 104-105 -0.43822620901945086 1102 106-107 -0.36338875105615287 1102 108-109 -0.3605619503904762 1102 110-111 -0.2487648010951098 1102 112-113 -0.31137310481944525 1102 114-115 -0.37129526560236314 1102 116-117 -0.33611112005216626 1102 118-119 -0.42242207039299373 1102 120-121 -0.5403819437485673 1102 122-123 -0.4903805481083232 1102 124-125 -0.5200274663327349 1103 1 0.23997303958422833 1103 2 0.14995398728045828 1103 3 0.25501842107298955 1103 4 0.3046840289606294 1103 5 0.21773107188383278 1103 6 0.1417863299886335 1103 7 0.11341380519924371 1103 8 0.18737477989603235 1103 9 0.3580625085816749 1103 10-11 0.4193771214086226 1103 12-13 0.3763537133575525 1103 14-15 0.4060991913436425 1103 16-17 0.25014513098518876 1103 18-19 0.2868175081890669 1103 20-21 0.3481598190272166 1103 22-23 0.33348676037702774 1103 24-25 0.3823425613264604 1103 26-27 0.3868396727017469 1103 28-29 0.28976031466473984 1103 30-31 0.41951575384388917 1103 32-33 0.4310156825961684 1103 34-35 0.4722612168136351 1103 36-37 0.2774144970050827 1103 38-39 0.17509653590905572 1103 40-41 0.23866566785525833 1103 42-43 0.3288524358563478 1103 44-45 0.1791804356675044 1103 46-47 0.2186283921073766 1103 48-49 0.26488348984680954 1103 50-51 0.20020579878853084 1103 52-53 0.1910501082911864 1103 54-55 0.24965315246784314 1103 56-57 0.19567835318093785 1103 58-59 0.18226460289318425 1103 60-61 0.18528048878108194 1103 62-63 0.09473656998074631 1103 64-65 0.13423318225473224 1103 66-67 0.05273146936576012 1103 68-69 0.08002065934106639 1103 70-71 0.2032347835459376 1103 72-73 0.12202225968879787 1103 74-75 0.04849741620800074 1103 76-77 0.07323458509387137 1103 78-79 0.12735695420087723 1103 80-81 0.13272612896882663 1103 82-83 0.11537184903234277 1103 84-85 -0.017847788819253196 1103 86-87 0.025076768658529147 1103 88-89 0.0446911977132487 1103 90-91 -0.019805848672529436 1103 92-93 0.03652048023870336 1103 94-95 -0.0829190682265768 1103 96-97 -0.1248731237553855 1103 98-99 -0.025565823948110022 1103 100-101 -0.1255951214599662 1103 102-103 -0.42489413997473946 1103 104-105 -0.20863366580384834 1103 106-107 -0.24019691500494034 1103 108-109 -0.12090938192820389 1103 110-111 -0.16602479671558612 1103 112-113 -0.21301574850648564 1103 114-115 -0.4480939298474098 1103 116-117 -0.4219393332061969 1103 118-119 -0.40280196193536 1103 120-121 -0.4886834289225881 1103 122-123 -0.503227689824584 1103 124-125 -0.7150926117587062 1104 1 0.1352254398372139 1104 2 0.16667402642393014 1104 3 0.167694356887786 1104 4 0.14200535046973783 1104 5 0.10812207875862612 1104 6 0.18599717156782702 1104 7 0.139853614155399 1104 8 0.023656764298984 1104 9 0.0917045026827239 1104 10-11 0.2992455771777571 1104 12-13 0.15336955286053922 1104 14-15 0.2119479618293738 1104 16-17 0.18635857591252858 1104 18-19 0.21391813752352817 1104 20-21 0.18445297983525677 1104 22-23 0.16653019284713366 1104 24-25 0.1773875878399167 1104 26-27 0.21648300961701494 1104 28-29 0.1855503479992251 1104 30-31 0.19121279016295745 1104 32-33 0.15807628257301332 1104 34-35 0.07526077195157654 1104 36-37 0.02588044969830605 1104 38-39 0.012014341197705392 1104 40-41 0.08167632808870451 1104 42-43 -0.004183934222808716 1104 44-45 -0.030935225726089755 1104 46-47 -0.06731938350002054 1104 48-49 -0.04987445253176048 1104 50-51 -0.1360564383465359 1104 52-53 -0.28620810149829623 1104 54-55 -0.2353387868071124 1104 56-57 -0.3240540356844548 1104 58-59 -0.22975891884554045 1104 60-61 -0.506066902961372 1104 62-63 -0.3423554226666923 1104 64-65 -0.2612216274940522 1104 66-67 -0.26121985197330133 1104 68-69 -0.3184251758230019 1104 70-71 -0.44644745000171326 1104 72-73 -0.24318926646234473 1104 74-75 -0.34079117201044795 1104 76-77 -0.3396870944990269 1104 78-79 -0.2731099589775212 1104 80-81 -0.26322263674465063 1104 82-83 -0.22408101177082074 1104 84-85 -0.30594856516115243 1104 86-87 -0.2456815182001577 1104 88-89 -0.25857239485234373 1104 90-91 -0.35395108269684883 1104 92-93 -0.42853666067505713 1104 94-95 -0.3900178133425527 1104 96-97 -0.46130424117099267 1104 98-99 -0.41347669739719706 1104 100-101 -0.5783037060909955 1104 102-103 -0.7924564823737121 1104 104-105 -0.6865235824648934 1104 106-107 -0.6152752184074437 1104 108-109 -0.6518083022463301 1104 110-111 -0.6747502778769672 1104 112-113 -0.6768910702909601 1104 114-115 -0.8113174957980753 1104 116-117 -0.8509899366772196 1104 118-119 -0.9074250167609073 1104 120-121 -0.8325535752772701 1104 122-123 -0.9108525076423213 1104 124-125 -1.0299294060750057 1105 1 -0.05910732578076505 1105 2 -0.09224031820250644 1105 3 -0.09217787549984635 1105 4 -0.03526812266086665 1105 5 -0.05603445239501781 1105 6 -0.028336744254126245 1105 7 -0.05501217603472952 1105 8 -0.028926024006501905 1105 9 0.016775704529806035 1105 10-11 -0.03539169439066825 1105 12-13 0.05286071950504123 1105 14-15 0.043662501779444085 1105 16-17 0.048590058683736004 1105 18-19 0.028999369360327876 1105 20-21 0.08464607428493309 1105 22-23 0.14144380883517726 1105 24-25 0.04463116358137853 1105 26-27 0.0922680370214195 1105 28-29 0.18605019868375905 1105 30-31 0.23966045007824022 1105 32-33 0.20777406384009822 1105 34-35 0.023722334561711023 1105 36-37 -0.05414941075768098 1105 38-39 -0.05513567494736549 1105 40-41 0.05902891731393112 1105 42-43 -0.05651056471609195 1105 44-45 -0.046565600819214126 1105 46-47 -0.008866070173908724 1105 48-49 -0.21828713411098022 1105 50-51 -0.20639886409507113 1105 52-53 -0.19346512354633916 1105 54-55 -0.14849093399523383 1105 56-57 -0.2160339218881262 1105 58-59 -0.19231773489521942 1105 60-61 -0.21860975861182652 1105 62-63 -0.19821263856103144 1105 64-65 -0.26952017770943115 1105 66-67 -0.39548308301234414 1105 68-69 -0.3645710427918871 1105 70-71 -0.30070928549510256 1105 72-73 -0.38190703173209783 1105 74-75 -0.4104627993274761 1105 76-77 -0.3885714889746694 1105 78-79 -0.23850814323808578 1105 80-81 -0.2544288524891485 1105 82-83 -0.23337609765623313 1105 84-85 -0.2483288859097783 1105 86-87 -0.28087907892354025 1105 88-89 -0.32577207290075094 1105 90-91 -0.29054351635963016 1105 92-93 -0.3300578318148588 1105 94-95 -0.32986010085556217 1105 96-97 -0.4483373401370727 1105 98-99 -0.4964476900970958 1105 100-101 -0.5067535002150372 1105 102-103 -0.6470087707520094 1105 104-105 -0.5841586602039079 1105 106-107 -0.5823493764897911 1105 108-109 -0.546829172117107 1105 110-111 -0.5584647218008278 1105 112-113 -0.5219675641315646 1105 114-115 -0.6719320977958425 1105 116-117 -0.626588372505335 1105 118-119 -0.632012961462479 1105 120-121 -0.7254504589631594 1105 122-123 -0.778175423715421 1105 124-125 -0.9381561213034928 1106 1 0.20817654182992484 1106 2 0.22198234381030701 1106 3 0.1826100408476954 1106 4 0.17892455141532793 1106 5 0.1493000872767567 1106 6 0.20955236810294764 1106 7 0.10658540875488143 1106 8 0.2429537202263603 1106 9 0.3363093494843312 1106 10-11 0.38475494929905096 1106 12-13 0.2926638466305107 1106 14-15 0.34596471538124973 1106 16-17 0.3557971635104522 1106 18-19 0.2883516591621813 1106 20-21 0.18552898881030444 1106 22-23 0.21029298096349436 1106 24-25 0.20319627613723412 1106 26-27 0.28914888966298236 1106 28-29 0.13605554584223967 1106 30-31 0.24849002164295797 1106 32-33 0.26855529952823076 1106 34-35 0.1824091284688265 1106 36-37 0.22988829803728095 1106 38-39 0.24693298110586426 1106 40-41 0.21989970268118242 1106 42-43 0.14445806268903993 1106 44-45 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2216 42-43 0.3268516059958415 2216 44-45 0.2743706705813267 2216 46-47 0.3371808002679728 2216 48-49 0.3299721520601935 2216 50-51 0.4037327407351583 2216 52-53 0.36606850712646377 2216 54-55 0.2949099396903563 2216 56-57 0.3212226665496303 2216 58-59 0.4226366036917355 2216 60-61 0.2400769329714052 2216 62-63 0.14150882648485208 2216 64-65 0.32447035680576874 2216 66-67 0.07154445233518913 2216 68-69 0.1800185029558392 2216 70-71 0.26403389121981746 2216 72-73 0.45164843639691554 2216 74-75 0.33631641340539886 2216 76-77 0.3525880312409342 2216 78-79 0.16973270641413052 2216 80-81 0.29127939620547494 2216 82-83 0.3139569299072207 2216 84-85 0.22447800133941342 2216 86-87 0.35325521092757484 2216 88-89 0.3100150972089999 2216 90-91 0.2821921639750222 2216 92-93 0.22149650958223432 2216 94-95 0.4235531307507401 2216 96-97 0.36875397532912757 2216 98-99 0.2875293992412402 2216 100-101 0.43434682308476624 2216 102-103 0.4247165977107379 2216 104-105 0.36782166815414286 2216 106-107 0.5589466696446586 2216 108-109 0.5972975872306208 2216 110-111 0.6540007703615629 2216 112-113 0.62005351458712 2216 114-115 0.6578684057543036 2216 116-117 0.7368190514804063 2216 118-119 0.7051136413203665 2216 120-121 0.8770829816907195 2216 122-123 0.844225532267096 2216 124-125 1.0688557609008313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20669.0 3 4291.0 4 2724.0 5 2326.0 6 2679.0 7 3366.0 8 3656.0 9 4012.0 10 4541.0 11 5107.0 12 5803.0 13 6013.0 14 5997.0 15 5717.0 16 5284.0 17 4602.0 18 4543.0 19 4344.0 20 4558.0 21 4718.0 22 5755.0 23 7350.0 24 10715.0 25 14867.0 26 17441.0 27 17745.0 28 20982.0 29 26022.0 30 31461.0 31 40179.0 32 52844.0 33 71291.0 34 102485.0 35 161030.0 36 321740.0 37 569830.0 38 222720.0 39 365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.794406466682155 18.778814523131107 13.122468741071458 24.304310269115277 2 15.414189346876633 20.486369647600103 39.41900136692487 24.680439638598404 3 18.485614588773934 25.466511829486482 29.679731516774645 26.36814206496494 4 12.423681385481515 16.627178428308845 37.735623651158086 33.21351653505155 5 12.995131146711927 39.15478351914558 34.519397331439365 13.330688002703129 6 31.658998878453975 37.11083520165042 17.812351678595366 13.417814241300238 7 28.371730951630838 32.99761463026769 21.36692298417366 17.263731433927816 8 26.613926652931593 34.8908639538786 20.374080716890806 18.121128676298998 9 27.108544860126727 16.108095914371376 19.45585329697317 37.32750592852872 10-11 24.52749517993966 26.992421253826876 28.070975735788462 20.409107830445 12-13 25.413368189881137 24.57642210131087 27.5943008988711 22.415908809936894 14-15 22.323593284358516 25.90808206722142 25.0152727975848 26.75305185083526 16-17 22.62284406659401 29.130586855053544 25.910178042045896 22.33639103630655 18-19 22.886578348023164 28.048929558260465 27.036661886428888 22.027830207287483 20-21 23.357041891972983 27.22314270918761 26.999669958194705 22.420145440644703 22-23 23.315231040376226 27.283483685711303 26.792088108938245 22.60919716497423 24-25 22.92748748174769 27.37382846271639 26.83895515654205 22.859728898993872 26-27 22.354748268113962 27.69567478547283 26.61767712799177 23.331899818421444 28-29 22.83191741166927 27.825611050300868 26.555337626474497 22.787133911555365 30-31 22.671245397331106 27.348312646442242 26.44980944797977 23.53063250824688 32-33 22.2452467563929 27.612985207562517 26.394913575115947 23.746854460928642 34-35 23.45153702395402 27.473683303972944 26.190290319184133 22.8844893528889 36-37 23.498919306355813 26.799952216116502 26.21484967523628 23.486278802291405 38-39 22.76203472552897 27.3773441176683 26.225253101644597 23.63536805515813 40-41 23.225111021284693 27.027574395026022 26.531732571012647 23.215582012676634 42-43 22.83972070743818 26.932207406370235 26.453643996718455 23.77442788947313 44-45 22.724629692961244 27.210069806665516 26.685721096159398 23.37957940421385 46-47 23.448192326583587 27.519735833205537 25.821187350397718 23.210884489813154 48-49 22.81950158131141 27.5301538194838 25.975679141580155 23.674665457624634 50-51 23.101898462694166 27.41074980608655 26.29294155037416 23.19441018084513 52-53 23.33875905224109 27.028803538691097 26.33507273047012 23.297364678597692 54-55 22.69137849413135 27.45681023619072 26.5319225306879 23.319888738990027 56-57 22.76226713665317 27.58516653257531 26.40593120891137 23.24663512186015 58-59 22.670763883043197 26.858020117699127 26.472440608003794 23.998775391253883 60-61 22.13116029319595 27.366909440856297 26.745490489800815 23.756439776146937 62-63 22.546389621704275 27.286087669661885 26.87011461553677 23.297408093097065 64-65 22.425810158239436 27.433586277136545 26.331788851840347 23.808814712783676 66-67 22.377499204044177 27.32323251967108 26.516691735339272 23.78257654094547 68-69 22.92030033577464 26.79848909746466 26.905089072238496 23.376121494522202 70-71 22.712166993195677 26.880634847961204 26.455045123234605 23.952153035608518 72-73 22.651645748973795 27.09127152253485 26.300811935239654 23.9562707932517 74-75 22.52781110065893 27.42745999263223 26.408863324574618 23.63586558213422 76-77 22.90250384358298 26.88874528893687 26.666353660752335 23.54239720672781 78-79 22.91386069956927 26.995348206625863 26.71022305493695 23.38056803886792 80-81 23.046906037348585 27.07780593605657 26.415268368276205 23.460019658318643 82-83 22.807306081196337 27.039812508438622 26.698183814406466 23.454697595958574 84-85 22.749632938699026 27.243463391799217 26.61991062734492 23.386993042156835 86-87 23.035282678149745 27.330684817335744 26.212418391443254 23.42161411307126 88-89 22.862297681670455 27.284171092864874 26.24783227880631 23.605698946658364 90-91 22.629070730743777 27.234050592048202 26.466146488799396 23.670732188408625 92-93 22.832540404636724 26.745723394058942 26.78628430140088 23.635451899903458 94-95 22.695778664067802 26.48344614454578 27.079191581998064 23.741583609388353 96-97 22.920005634051673 26.29685284873821 26.952047497166998 23.83109402004311 98-99 22.725531339759907 26.569168833845946 26.656957999829423 24.048341826564723 100-101 22.4603229945912 26.508854139029747 27.02300845422271 24.007814412156346 102-103 22.31450373705408 26.309705283494093 27.091954339124985 24.283836640326847 104-105 22.250216765555102 26.664899759995176 26.688930801989493 24.39595267246023 106-107 22.429742440811175 26.69856706585229 26.389203762480108 24.482486730856426 108-109 22.46835996916217 26.516922606769043 26.571847283284367 24.44287014078442 110-111 22.582548660460404 26.62496252934102 26.770511209526482 24.021977600672095 112-113 22.56827229293833 26.97514368179334 26.85884803971277 23.59773598555556 114-115 22.499365176558705 27.158913671235357 26.542871002209793 23.798850149996138 116-117 22.38487888494695 27.07831986375772 26.691123971029068 23.845677280266262 118-119 22.20094486298153 27.36823030243064 26.40132718840728 24.029497646180552 120-121 22.276353324640375 27.57806784565282 26.50960064527584 23.635978184430968 122-123 22.270060774440008 27.59076228511894 26.365132835561727 23.77404410487932 124-125 22.286442177730674 27.5016217823075 26.51796740321745 23.69396863674438 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 582.0 1 553.5 2 493.0 3 401.5 4 334.5 5 310.0 6 264.5 7 234.5 8 220.0 9 211.5 10 207.5 11 185.0 12 183.0 13 210.0 14 227.0 15 241.5 16 282.5 17 335.5 18 416.5 19 509.0 20 655.0 21 911.5 22 1258.0 23 1809.5 24 2657.0 25 4024.0 26 6090.0 27 8725.0 28 11571.5 29 14774.5 30 18394.5 31 23950.0 32 32581.5 33 35800.5 34 34378.0 35 35808.5 36 39525.0 37 43965.0 38 47227.5 39 49859.0 40 52678.0 41 56381.0 42 58093.5 43 59556.0 44 61512.0 45 64616.0 46 66351.0 47 67076.0 48 68227.0 49 67818.0 50 67321.5 51 65211.0 52 63947.5 53 65803.5 54 68812.5 55 66158.5 56 60447.0 57 54794.0 58 50298.5 59 44916.5 60 35048.5 61 28001.5 62 24178.0 63 17372.5 64 11514.0 65 9058.5 66 6550.0 67 4955.0 68 3929.5 69 3020.5 70 2074.5 71 1163.0 72 865.0 73 617.5 74 410.5 75 293.0 76 176.0 77 112.0 78 79.0 79 57.5 80 46.0 81 31.0 82 22.0 83 13.0 84 11.5 85 10.0 86 9.5 87 8.5 88 7.5 89 6.5 90 5.0 91 3.0 92 2.0 93 3.0 94 2.5 95 4.5 96 5.0 97 2.5 98 2.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04256094660879267 2 0.006223010470215115 3 0.0017780029914900332 4 0.004445007478725083 5 0.020835972556523825 6 0.026114418937509862 7 0.0028336922676872405 8 0.0 9 0.0 10-11 1.1112518696812707E-4 12-13 8.056576055189212E-4 14-15 1.9446907719422235E-4 16-17 2.778129674203177E-5 18-19 5.556259348406354E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1112518696812707E-4 30-31 5.556259348406354E-5 32-33 5.556259348406354E-5 34-35 6.389698250667307E-4 36-37 1.1112518696812707E-4 38-39 5.556259348406354E-5 40-41 2.500316706782859E-4 42-43 5.834072315826671E-4 44-45 8.612201990029849E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.834072315826671E-4 54-55 0.0022225037393625415 56-57 2.2225037393625415E-4 58-59 7.500950120348577E-4 60-61 0.0011112518696812707 62-63 8.890014957450166E-4 64-65 0.0017224403980059698 66-67 0.002722567080719113 68-69 0.0029448174546553674 70-71 0.0018891281784581603 72-73 0.00330597431230178 74-75 0.002278066332846605 76-77 0.0010556892761972073 78-79 0.0020002533654262873 80-81 0.0013890648371015883 82-83 6.111885283246989E-4 84-85 2.500316706782859E-4 86-87 0.0012223770566493978 88-89 0.003944944137368511 90-91 1.666877804521906E-4 92-93 5.278446380986037E-4 94-95 5.556259348406354E-4 96-97 8.33438902260953E-5 98-99 3.0559426416234945E-4 100-101 0.0011390331664233025 102-103 0.0014168461338436202 104-105 9.167827924870484E-4 106-107 0.006000760096278862 108-109 0.0019169094752001922 110-111 0.0016946591012639378 112-113 0.0026947857839770815 114-115 0.003278193015559749 116-117 0.0020558159589103508 118-119 0.043394385511053624 120-121 0.01286274039156071 122-123 0.004695039149403369 124-125 0.003028161344881463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1799772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.13587861287725 #Duplication Level Percentage of deduplicated Percentage of total 1 79.34896452159165 49.30417627571122 2 11.424446931365964 14.197360954932266 3 3.9095684857982356 7.287734185868685 4 1.7612112656063261 4.377376376453864 5 0.9722357837701421 3.020536232171856 6 0.632753373786947 2.3590012095308563 7 0.40504257367453983 1.7617373333622015 8 0.26941107357025706 1.3392075011461149 9 0.20485593726062828 1.1456013285678235 >10 0.9977283488447359 10.852324127080616 >50 0.05362356290622883 2.2762227018916477 >100 0.01975497899101123 1.9116324245460397 >500 4.031628333151107E-4 0.16708934873684952 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.556259348406354E-5 2 0.0 0.0 0.0 0.0 5.556259348406354E-5 3 0.0 0.0 0.0 0.0 5.556259348406354E-5 4 0.0 0.0 0.0 0.0 5.556259348406354E-5 5 0.0 0.0 0.0 0.0 5.556259348406354E-5 6 0.0 0.0 0.0 0.0 5.556259348406354E-5 7 0.0 0.0 0.0 0.0 5.556259348406354E-5 8 0.0 0.0 0.0 0.0 5.556259348406354E-5 9 0.0 0.0 0.0 0.0 5.556259348406354E-5 10-11 0.0 0.0 0.0 0.0 5.556259348406354E-5 12-13 0.0 0.0 0.0 0.0 1.1112518696812707E-4 14-15 0.0 0.0 0.0 0.0 1.3890648371015884E-4 16-17 0.0 0.0 0.0 0.0 1.9446907719422238E-4 18-19 0.0 0.0 0.0 0.0 2.2225037393625415E-4 20-21 0.0 0.0 0.0 0.0 2.500316706782859E-4 22-23 0.0 0.0 0.0 1.3890648371015884E-4 2.778129674203177E-4 24-25 0.0 0.0 0.0 5.278446380986037E-4 2.778129674203177E-4 26-27 0.0 0.0 0.0 0.0012223770566493978 2.778129674203177E-4 28-29 0.0 0.0 0.0 0.0042505384015308605 2.778129674203177E-4 30-31 0.0 0.0 0.0 0.014390711712372457 2.778129674203177E-4 32-33 0.0 0.0 0.0 0.03933831618671699 2.778129674203177E-4 34-35 0.0 0.0 0.0 0.07603740918294094 2.778129674203177E-4 36-37 0.0 0.0 0.0 0.13718404331215286 2.778129674203177E-4 38-39 0.0 0.0 0.0 0.23239054724709574 3.0559426416234945E-4 40-41 0.0 0.0 0.0 0.34120988658563417 3.333755609043812E-4 42-43 0.0 0.0 0.0 0.45022369500136683 3.333755609043812E-4 44-45 0.0 0.0 0.0 0.5837961697370556 3.61156857646413E-4 46-47 5.556259348406354E-5 0.0 0.0 0.7416494978252801 4.1671945113047653E-4 48-49 5.556259348406354E-5 0.0 0.0 0.907809433639372 4.445007478725083E-4 50-51 5.556259348406354E-5 0.0 0.0 1.1001115696877162 4.445007478725083E-4 52-53 5.556259348406354E-5 0.0 0.0 1.275022613975548 5.000633413565718E-4 54-55 5.556259348406354E-5 0.0 0.0 1.4649911210975612 5.000633413565718E-4 56-57 1.1112518696812707E-4 0.0 0.0 1.6838243955345455 5.278446380986037E-4 58-59 1.1112518696812707E-4 0.0 0.0 1.9285220572383612 5.556259348406354E-4 60-61 1.1112518696812707E-4 0.0 0.0 2.195667006709739 5.556259348406354E-4 62-63 1.1112518696812707E-4 0.0 0.0 2.460700577628722 5.556259348406354E-4 64-65 1.1112518696812707E-4 0.0 0.0 2.733512911635474 5.556259348406354E-4 66-67 1.1112518696812707E-4 0.0 0.0 3.0516643219252213 5.556259348406354E-4 68-69 1.1112518696812707E-4 0.0 0.0 3.4107931449094666 5.556259348406354E-4 70-71 1.1112518696812707E-4 0.0 0.0 3.784284898309341 5.556259348406354E-4 72-73 1.1112518696812707E-4 0.0 0.0 4.189169517027713 5.834072315826671E-4 74-75 1.1112518696812707E-4 0.0 0.0 4.590720380137039 6.111885283246989E-4 76-77 1.1112518696812707E-4 0.0 0.0 5.025025392105222 6.111885283246989E-4 78-79 1.1112518696812707E-4 0.0 0.0 5.503780478860656 6.111885283246989E-4 80-81 1.1112518696812707E-4 0.0 0.0 6.013650617967165 6.111885283246989E-4 82-83 1.1112518696812707E-4 0.0 0.0 6.555497029623752 6.111885283246989E-4 84-85 1.1112518696812707E-4 0.0 0.0 7.09542653180514 6.111885283246989E-4 86-87 1.1112518696812707E-4 0.0 0.0 7.643218140964523 6.111885283246989E-4 88-89 1.1112518696812707E-4 0.0 0.0 8.240904959072594 6.111885283246989E-4 90-91 1.666877804521906E-4 0.0 0.0 8.884903198849631 6.111885283246989E-4 92-93 1.666877804521906E-4 0.0 0.0 9.543486619416237 7.778763087768895E-4 94-95 1.666877804521906E-4 0.0 0.0 10.203292417039492 7.778763087768895E-4 96-97 1.666877804521906E-4 5.556259348406354E-5 0.0 10.875960955054307 8.612201990029849E-4 98-99 1.666877804521906E-4 5.556259348406354E-5 0.0 11.568048619491801 8.890014957450166E-4 100-101 1.666877804521906E-4 5.556259348406354E-5 0.0 12.324338860700134 8.890014957450166E-4 102-103 1.666877804521906E-4 5.556259348406354E-5 0.0 13.127134992654625 9.167827924870483E-4 104-105 1.666877804521906E-4 5.556259348406354E-5 0.0 13.927208557528399 9.445640892290801E-4 106-107 1.666877804521906E-4 5.556259348406354E-5 0.0 14.719420015424177 9.445640892290801E-4 108-109 1.666877804521906E-4 5.556259348406354E-5 0.0 15.537162485025881 0.0010001266827131437 110-111 1.666877804521906E-4 5.556259348406354E-5 0.0 16.406300353600344 0.001055689276197207 112-113 1.666877804521906E-4 5.556259348406354E-5 0.0 17.295496318422558 0.001055689276197207 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1725 0.0 59.020836 1 GTATCAA 3030 0.0 53.840153 1 TCAACGC 3800 0.0 42.290783 4 ATCAACG 3855 0.0 41.841812 3 CAACGCA 3965 0.0 40.682133 5 TATCAAC 3965 0.0 40.681 2 AACGCAG 4270 0.0 37.494347 6 GTACATG 9215 0.0 36.440315 1 TACATGG 9270 0.0 35.635166 2 ACATGGG 9500 0.0 33.70732 3 ACGCAGA 5005 0.0 31.738005 7 CGCAGAG 5075 0.0 31.30198 8 CATGGGA 6250 0.0 30.188725 4 AGGTAAG 1160 0.0 29.247074 2 TAAGGTA 770 0.0 28.600693 4 CATGGGG 3740 0.0 27.532145 4 TAAGGTG 1495 0.0 27.471699 5 GTAAGGT 1435 0.0 26.545666 4 ATGGGAT 2410 0.0 25.932829 5 GGTAAGG 1400 0.0 25.508728 3 >>END_MODULE