FastQCFastQC Report
Thu 26 May 2016
SRR1364367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799772
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.20091433803837377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33000.18335655849740967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.10968055953754142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.080.052611
GTATCAA54200.059.8862151
ATCAACG73750.043.51953
TATCAAC74100.043.4746632
TCAACGC73800.043.409334
CAACGCA74350.043.088215
AACGCAG78700.040.832946
ACGCAGA93050.034.464147
CGCAGAG94200.034.04348
GTACATG86600.033.491971
TACATGG88150.032.694922
TGGTATC9850.030.831252
ACATGGG90450.030.678533
CATGGGA59650.029.1493974
GCAGAGT110250.028.5477689
AGGTAAG11650.027.0898822
TAAGGTG14550.027.0108415
GAGTACT59500.025.89869312-13
CAGAGTA102050.025.62364810-11
AGAGTAC99650.025.37503810-11
Produced by FastQC (version 0.11.5)