##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364367_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1799772 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.243359158826784 33.0 31.0 34.0 30.0 34.0 2 32.27369300111347 33.0 31.0 34.0 30.0 34.0 3 32.37891077314237 34.0 31.0 34.0 30.0 34.0 4 35.99383588587888 37.0 35.0 37.0 35.0 37.0 5 35.93201249936103 37.0 35.0 37.0 35.0 37.0 6 35.999951660543665 37.0 35.0 37.0 35.0 37.0 7 35.94887407960564 37.0 35.0 37.0 35.0 37.0 8 35.95195391416246 37.0 35.0 37.0 35.0 37.0 9 37.69491802294957 39.0 37.0 39.0 35.0 39.0 10-11 37.644313835308026 39.0 37.0 39.0 35.0 39.0 12-13 37.62888604778827 39.0 37.0 39.0 35.0 39.0 14-15 39.00616078036551 40.5 38.0 41.0 35.0 41.0 16-17 39.0140570583385 40.5 38.0 41.0 35.0 41.0 18-19 39.08046463663175 41.0 38.5 41.0 35.0 41.0 20-21 39.08396535783422 41.0 39.0 41.0 35.0 41.0 22-23 39.04861754711152 41.0 39.0 41.0 35.0 41.0 24-25 39.01160813703069 41.0 38.5 41.0 35.0 41.0 26-27 38.90805752061928 41.0 38.0 41.0 35.0 41.0 28-29 38.81825003389318 40.0 38.0 41.0 35.0 41.0 30-31 38.692643845998276 40.0 38.0 41.0 34.0 41.0 32-33 38.55740393783212 40.0 38.0 41.0 34.0 41.0 34-35 38.45659450197025 40.0 38.0 41.0 34.0 41.0 36-37 38.328729139024276 40.0 38.0 41.0 34.0 41.0 38-39 38.229332659914704 40.0 38.0 41.0 33.5 41.0 40-41 38.114477278233025 40.0 38.0 41.0 33.0 41.0 42-43 37.95193474506771 40.0 38.0 41.0 33.0 41.0 44-45 37.77774240292659 40.0 37.0 41.0 33.0 41.0 46-47 37.54745434421693 40.0 37.0 41.0 32.5 41.0 48-49 37.31938962268554 40.0 37.0 41.0 32.0 41.0 50-51 37.14388461427336 40.0 36.5 41.0 31.0 41.0 52-53 36.970165943241696 40.0 36.0 41.0 31.0 41.0 54-55 36.7775479338494 39.5 36.0 41.0 31.0 41.0 56-57 36.567744136479504 39.0 35.0 41.0 31.0 41.0 58-59 36.33924713797081 39.0 35.0 41.0 30.5 41.0 60-61 36.07781985718191 39.0 35.0 41.0 30.0 41.0 62-63 35.81889344872573 38.0 35.0 40.5 30.0 41.0 64-65 35.50319901631984 37.0 35.0 40.0 29.0 41.0 66-67 35.173503921607846 37.0 34.5 40.0 29.0 41.0 68-69 34.81901429736656 36.0 34.0 39.0 29.0 41.0 70-71 34.545080710223296 36.0 34.0 39.0 29.0 41.0 72-73 34.3505491251114 35.5 34.0 39.0 29.0 40.5 74-75 34.02684534485479 35.0 34.0 37.5 29.0 40.0 76-77 33.7105686164692 35.0 34.0 37.0 29.0 39.0 78-79 33.40070242230682 35.0 34.0 36.5 29.0 39.0 80-81 33.12526447794498 35.0 34.0 36.0 28.0 38.5 82-83 32.857431663566274 35.0 34.0 36.0 28.0 37.0 84-85 32.6459623774567 35.0 33.5 35.0 27.0 37.0 86-87 32.47601085026326 35.0 33.0 35.0 27.0 36.5 88-89 32.32086536516847 35.0 33.0 35.0 27.0 36.0 90-91 32.18807493393608 35.0 33.0 35.0 27.0 36.0 92-93 32.06786026229989 35.0 33.0 35.0 26.5 36.0 94-95 31.98501449072438 35.0 33.0 35.0 26.0 35.0 96-97 31.90084105097757 35.0 33.0 35.0 26.0 35.0 98-99 31.83130252054149 35.0 33.0 35.0 25.0 35.0 100-101 31.772487015021902 35.0 33.0 35.0 25.0 35.0 102-103 30.78642628066222 34.0 31.5 35.0 24.0 35.0 104-105 31.511778714192687 35.0 32.5 35.0 25.0 35.0 106-107 31.81486016006472 35.0 33.0 35.0 25.5 35.0 108-109 31.838125329208367 35.0 33.0 35.0 26.0 35.0 110-111 31.795798856744078 35.0 33.0 35.0 25.0 35.0 112-113 31.718870501374617 35.0 33.0 35.0 25.0 35.0 114-115 31.63364220579051 35.0 33.0 35.0 25.0 35.0 116-117 31.559826189095062 35.0 33.0 35.0 25.0 35.0 118-119 31.44178484830301 35.0 33.0 35.0 25.0 35.0 120-121 31.293813883091858 35.0 33.0 35.0 24.0 35.0 122-123 31.14128817427985 35.0 33.0 35.0 23.5 35.0 124-125 30.42252157495505 34.5 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1530982537536687 1101 2 0.18005644383622865 1101 3 0.14432527835849385 1101 4 0.14243708675335398 1101 5 0.20288905962167547 1101 6 0.24405883926295502 1101 7 0.24062646096085416 1101 8 0.26246709169187454 1101 9 0.16208795135027998 1101 10-11 0.2720692410224501 1101 12-13 0.2274677493782562 1101 14-15 0.2956713769850623 1101 16-17 0.34041779979660447 1101 18-19 0.3437285347635637 1101 20-21 0.34057083247885345 1101 22-23 0.3392751694593912 1101 24-25 0.3261660497677852 1101 26-27 0.3126961264477188 1101 28-29 0.3028046253454235 1101 30-31 0.47695612968832535 1101 32-33 0.3266957073694172 1101 34-35 0.281078093958925 1101 36-37 0.4367250759860042 1101 38-39 0.44675454586377583 1101 40-41 0.4378051162242045 1101 42-43 0.41660427167812486 1101 44-45 0.3539440258590929 1101 46-47 0.40115855103891107 1101 48-49 0.21939529661673163 1101 50-51 0.35424643758337737 1101 52-53 0.4596490846243313 1101 54-55 0.4456342468806085 1101 56-57 0.47341930279530686 1101 58-59 0.33668322556538754 1101 60-61 0.4665796253634866 1101 62-63 0.3128489638997536 1101 64-65 0.25393031387250176 1101 66-67 0.1893866167681253 1101 68-69 0.28531281933595665 1101 70-71 0.347202270064777 1101 72-73 0.3333664434612231 1101 74-75 0.27317338696490623 1101 76-77 0.2555130945383439 1101 78-79 -0.04036016637232365 1101 80-81 0.0012158653491738391 1101 82-83 0.03857299325591157 1101 84-85 -0.13425853429819767 1101 86-87 -0.2027886036406059 1101 88-89 -0.1789934331133196 1101 90-91 -0.5375616436605206 1101 92-93 -0.39257246861892625 1101 94-95 -0.1911240663801479 1101 96-97 -0.0195310224298062 1101 98-99 0.052890531990737344 1101 100-101 -0.13461089147195082 1101 102-103 0.05450348973522523 1101 104-105 0.016370182913583164 1101 106-107 0.02695366562599233 1101 108-109 0.1231531121420062 1101 110-111 0.05835286236634474 1101 112-113 0.007061985150897954 1101 114-115 -0.11030342126097636 1101 116-117 -0.007231429069765483 1101 118-119 -0.008250706908217609 1101 120-121 -0.04627467885272196 1101 122-123 -0.02561964667541261 1101 124-125 0.06509888407507347 1102 1 0.15608965215258053 1102 2 0.14039350547630391 1102 3 0.10111145902050822 1102 4 0.12004240668749588 1102 5 0.10120517082741287 1102 6 0.08272795677086009 1102 7 0.211737997893799 1102 8 0.22968354166253846 1102 9 0.2775530907359638 1102 10-11 0.28403128610278827 1102 12-13 0.22169135788711714 1102 14-15 0.3172215104092331 1102 16-17 0.31743288335230346 1102 18-19 0.3527459035631253 1102 20-21 0.3645327734694135 1102 22-23 0.32754022935832694 1102 24-25 0.3773113922231701 1102 26-27 0.39634350338520363 1102 28-29 0.4301827220164469 1102 30-31 0.4636965108461837 1102 32-33 0.433145252213194 1102 34-35 0.4404484217471136 1102 36-37 0.4664965279438391 1102 38-39 0.44998192053021313 1102 40-41 0.4960267896093953 1102 42-43 0.4443589841064366 1102 44-45 0.440413063880257 1102 46-47 0.4391655155916112 1102 48-49 0.3600205923263431 1102 50-51 0.2954321621428875 1102 52-53 0.30599009221342044 1102 54-55 0.3711065542671932 1102 56-57 0.31931024036036604 1102 58-59 0.42111778148051826 1102 60-61 0.39809623714645426 1102 62-63 0.34543320565300917 1102 64-65 0.41006557854443315 1102 66-67 0.34245241570452123 1102 68-69 0.25750487917811427 1102 70-71 0.2650097844007817 1102 72-73 0.34752206241691397 1102 74-75 0.28107770479832084 1102 76-77 0.4049736019287238 1102 78-79 0.2895701407871556 1102 80-81 0.31453084103001316 1102 82-83 0.23338411761481126 1102 84-85 0.22538940567038424 1102 86-87 0.24756057026502987 1102 88-89 0.20896988470117606 1102 90-91 0.21993991768621157 1102 92-93 0.2023284331770938 1102 94-95 0.09306587854689496 1102 96-97 0.1559933298478704 1102 98-99 0.24321515017049578 1102 100-101 0.1436205048451633 1102 102-103 0.21596921580876938 1102 104-105 0.17954037917013466 1102 106-107 0.11387868786335176 1102 108-109 0.17501170617292772 1102 110-111 0.0836005487343705 1102 112-113 -0.04546550401429883 1102 114-115 -0.09281929495275776 1102 116-117 -0.17441026477823485 1102 118-119 -0.1901588382127457 1102 120-121 -0.1825589575105333 1102 122-123 -0.1514322566793851 1102 124-125 -0.3291642312374812 1103 1 0.17871021788408825 1103 2 0.17259589102902595 1103 3 0.19198981128884185 1103 4 0.13078351238860364 1103 5 0.00661066326629367 1103 6 0.12013263770962368 1103 7 0.12281594054779532 1103 8 0.18089048707305722 1103 9 0.30453385947131295 1103 10-11 0.27760852030429817 1103 12-13 0.23256368830936225 1103 14-15 0.3584164121275535 1103 16-17 0.31697560947745984 1103 18-19 0.2903207741095315 1103 20-21 0.23246112960203646 1103 22-23 0.2771966299044877 1103 24-25 0.2684504228025517 1103 26-27 0.2659713404153692 1103 28-29 0.25332158514342495 1103 30-31 0.2705981261178394 1103 32-33 0.1795144332421188 1103 34-35 0.2781218315933103 1103 36-37 0.3546310759511542 1103 38-39 0.3280999165113556 1103 40-41 0.3449782799357948 1103 42-43 0.2987824647978883 1103 44-45 0.2336431563655026 1103 46-47 0.26089310917173947 1103 48-49 0.26797007322388566 1103 50-51 0.1821490001262447 1103 52-53 0.26133021380962873 1103 54-55 0.27800704245536423 1103 56-57 0.18618960378360327 1103 58-59 0.2216867976332395 1103 60-61 0.19130942337928758 1103 62-63 0.16004326696635474 1103 64-65 0.16762428349328928 1103 66-67 0.1416987188706571 1103 68-69 0.18928815068287008 1103 70-71 0.19087103720239185 1103 72-73 0.2316377527072575 1103 74-75 0.26940192485654535 1103 76-77 0.19401174887464379 1103 78-79 0.23142390331388896 1103 80-81 0.24579162882918126 1103 82-83 0.17174875166690384 1103 84-85 0.15730873225409425 1103 86-87 0.1632160820253432 1103 88-89 0.13792739687367828 1103 90-91 0.2597452864679326 1103 92-93 0.14369352450305684 1103 94-95 0.15321832464010043 1103 96-97 0.0523475367675168 1103 98-99 0.07562266491918379 1103 100-101 0.12456876849253717 1103 102-103 0.18425469639717207 1103 104-105 0.2519732049938739 1103 106-107 0.24024809430898486 1103 108-109 0.25753434444997225 1103 110-111 0.24680062859629004 1103 112-113 0.21208917563637186 1103 114-115 0.2810914593559204 1103 116-117 0.23845813051248754 1103 118-119 0.09749299603513606 1103 120-121 -0.010643301998772614 1103 122-123 0.026381265746355353 1103 124-125 -0.06781530550906467 1104 1 0.07977558298639309 1104 2 0.07435062776777812 1104 3 0.1236368376519934 1104 4 0.12683693862186374 1104 5 0.1533515138359789 1104 6 0.10939122105159527 1104 7 0.14776099103222862 1104 8 0.1756172808792158 1104 9 0.1634403161148157 1104 10-11 0.1716311731632345 1104 12-13 0.2286910296633735 1104 14-15 0.1766779860835399 1104 16-17 0.2216013795523466 1104 18-19 0.18739547692685932 1104 20-21 0.09846919745803717 1104 22-23 0.20873429205835237 1104 24-25 0.15502588726103994 1104 26-27 0.12478975715622909 1104 28-29 0.11786437829573515 1104 30-31 0.11082949553053112 1104 32-33 0.14199126448581012 1104 34-35 0.14973181537312286 1104 36-37 0.18880483380686286 1104 38-39 0.18692662984739883 1104 40-41 0.08797012541123905 1104 42-43 0.10008442685351326 1104 44-45 0.11135278321344799 1104 46-47 0.20494100836413764 1104 48-49 0.11483035362412153 1104 50-51 0.04204165452656383 1104 52-53 0.12350792879840355 1104 54-55 0.06343686368224866 1104 56-57 0.08006182085873803 1104 58-59 0.0937068114892341 1104 60-61 0.13142802766433448 1104 62-63 -0.034333616223619856 1104 64-65 0.017673747656658634 1104 66-67 0.03455703716041114 1104 68-69 0.01539556100969719 1104 70-71 0.020137324721510197 1104 72-73 0.11984298386822445 1104 74-75 0.1896356474853249 1104 76-77 0.22721102020045691 1104 78-79 0.23836136536210262 1104 80-81 0.160020467848895 1104 82-83 0.10225818455279523 1104 84-85 0.1954692989348601 1104 86-87 0.1640868816854706 1104 88-89 0.05839198426274095 1104 90-91 0.14962985820179853 1104 92-93 0.20294890439935642 1104 94-95 0.17197761202702821 1104 96-97 0.10718131139743647 1104 98-99 0.06161280115887635 1104 100-101 0.08128645990527161 1104 102-103 0.06583129083820793 1104 104-105 0.044831564423773784 1104 106-107 0.1139747383650267 1104 108-109 0.20843557410137237 1104 110-111 0.22646457672240672 1104 112-113 0.20715427853883384 1104 114-115 0.2623027567678804 1104 116-117 0.1404651248850648 1104 118-119 0.07640983919300837 1104 120-121 0.07012117233718484 1104 122-123 -0.1389656251834488 1104 124-125 -0.3760309368977879 1105 1 0.14586812375532077 1105 2 0.18673729574293674 1105 3 0.14721984439071178 1105 4 0.18710802270494042 1105 5 0.12853691827513103 1105 6 0.1476563441234191 1105 7 0.24203089114387666 1105 8 0.2312012837578905 1105 9 0.16209937877243874 1105 10-11 0.23476042179513001 1105 12-13 0.23160314299961726 1105 14-15 0.2890720406897387 1105 16-17 0.3431973297791089 1105 18-19 0.3458138488500708 1105 20-21 0.28194282401951654 1105 22-23 0.31644832233087783 1105 24-25 0.3749449535275531 1105 26-27 0.3132115031869702 1105 28-29 0.3583811311749372 1105 30-31 0.45116120011172 1105 32-33 0.3696292942391608 1105 34-35 0.33537110982323526 1105 36-37 0.2314230568366895 1105 38-39 0.38308666331939634 1105 40-41 0.42951871150452803 1105 42-43 0.3338023134750614 1105 44-45 0.3157413917621028 1105 46-47 0.26770092544392554 1105 48-49 0.2591960557022972 1105 50-51 0.3050249116720849 1105 52-53 0.3763389870493796 1105 54-55 0.3277889558927285 1105 56-57 0.33660011051597394 1105 58-59 0.17994546509634546 1105 60-61 0.1512029622926505 1105 62-63 0.2117882705751981 1105 64-65 0.125736730293859 1105 66-67 0.1296432919063264 1105 68-69 0.11611326454831783 1105 70-71 0.2837709351590334 1105 72-73 0.3502942648155738 1105 74-75 0.32819585327685985 1105 76-77 0.2447619853165648 1105 78-79 0.3734764991973094 1105 80-81 0.36854387502744146 1105 82-83 0.2210811143439244 1105 84-85 0.25650311862508346 1105 86-87 0.2768808696009444 1105 88-89 0.2886513447071266 1105 90-91 0.19083024378394242 1105 92-93 0.186358069414581 1105 94-95 0.21847473968278308 1105 96-97 0.23408270937011721 1105 98-99 0.18113020617208164 1105 100-101 0.2434352783608773 1105 102-103 0.27162698664829676 1105 104-105 0.20727479288804673 1105 106-107 0.17819997837777635 1105 108-109 0.16747874948742947 1105 110-111 0.16235897375171504 1105 112-113 0.13051204579242182 1105 114-115 0.13289992895446545 1105 116-117 0.155664569199363 1105 118-119 0.028588064354405418 1105 120-121 0.1441056029017993 1105 122-123 0.036804932338359464 1105 124-125 -0.03496076706858631 1106 1 0.16135576024792897 1106 2 0.1572600797537831 1106 3 0.1602962656386353 1106 4 0.14725926811783552 1106 5 0.1014091633313825 1106 6 0.07027747979654464 1106 7 0.12062786009673943 1106 8 0.08537233533811417 1106 9 0.13572875223650271 1106 10-11 0.15175248558880128 1106 12-13 0.20171478046275837 1106 14-15 0.18563789819368282 1106 16-17 0.1420285797912939 1106 18-19 0.1120452763050821 1106 20-21 0.1775256082642258 1106 22-23 0.04924490570367368 1106 24-25 0.1275291901530835 1106 26-27 0.12444816086944144 1106 28-29 0.10780049553786597 1106 30-31 0.11534363054263252 1106 32-33 0.16971036641873383 1106 34-35 0.2056391181760091 1106 36-37 0.27331505933901923 1106 38-39 0.11466722490013836 1106 40-41 0.006485881169503216 1106 42-43 0.023544953154043924 1106 44-45 0.0660834150196905 1106 46-47 0.07639926629007476 1106 48-49 0.06514980607013854 1106 50-51 0.11009765982408481 1106 52-53 0.019800082147433784 1106 54-55 0.10930077177312825 1106 56-57 0.024902976008263522 1106 58-59 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-0.9845366361434529 2216 86-87 -1.1127146242742931 2216 88-89 -1.0378458237433144 2216 90-91 -0.9796242560514656 2216 92-93 -1.0052916527831073 2216 94-95 -1.2082115778843345 2216 96-97 -1.0807216045946646 2216 98-99 -1.047453022115583 2216 100-101 -1.1738862510505754 2216 102-103 -1.1124679110760276 2216 104-105 -1.0792869552325044 2216 106-107 -0.9591351099542607 2216 108-109 -0.8450502049802893 2216 110-111 -0.8965969893632035 2216 112-113 -0.8006825375977833 2216 114-115 -0.8963658285653295 2216 116-117 -0.8270258110258233 2216 118-119 -0.9007127697216042 2216 120-121 -0.7808179379000819 2216 122-123 -0.8703442285006169 2216 124-125 -0.7256174898577434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 229.0 3 9.0 4 43.0 5 124.0 6 288.0 7 689.0 8 1288.0 9 2178.0 10 3599.0 11 4666.0 12 6211.0 13 8317.0 14 12298.0 15 10168.0 16 5456.0 17 4795.0 18 4813.0 19 4702.0 20 4538.0 21 4864.0 22 5164.0 23 5549.0 24 7021.0 25 9670.0 26 12489.0 27 16364.0 28 20920.0 29 26383.0 30 33530.0 31 42150.0 32 53556.0 33 69220.0 34 94882.0 35 145786.0 36 295097.0 37 607181.0 38 274656.0 39 879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28151494722661 19.11924490509349 13.477041311134293 24.122198836545607 2 15.940685820203893 20.98126873848465 38.39208521968338 24.685960221628072 3 18.667753470995216 25.61691147545356 30.339398546038055 25.375936507513174 4 12.92208124140169 17.134559266395964 37.252218614357815 32.691140877844525 5 12.717750471412813 38.9208537908706 34.62860675276622 13.732788984950368 6 31.445739479706276 37.52974400979816 17.26108377669181 13.763432733803754 7 27.748848187437076 33.287938694456855 21.490110969611706 17.473102148494366 8 26.78689300644748 34.260339643021446 20.30346066057256 18.649306689958507 9 27.17271965560082 15.11558130696555 19.77483814616518 37.936860891268445 10-11 24.656928766532648 26.48040974078939 28.11592246128954 20.74673903138842 12-13 25.980540868510012 24.072076907519396 28.01288163167335 21.934500592297248 14-15 22.585916438304405 25.15996470664062 26.03135286025119 26.222765994803787 16-17 22.030929473288836 28.61470786299598 26.896823597655704 22.45753906605948 18-19 22.35130338731795 26.775808269047413 29.113715515076354 21.759172828558285 20-21 22.750312332183093 25.98384290406561 29.385673085925312 21.880171677825984 22-23 22.73882469557255 26.046660354756046 29.13966324623341 22.074851703437993 24-25 22.442287134148103 26.22471068557573 29.11157635522722 22.22142582504895 26-27 21.837075725269305 26.59067515852786 28.96123794111723 22.6110111750856 28-29 22.263480626391846 26.69188473180988 28.875443946108607 22.169190695689668 30-31 22.160698911721752 26.226552900246393 28.764850165535087 22.84789802249677 32-33 21.833990408216163 26.416271639229354 28.71153778303277 23.03820016952171 34-35 22.860051167691843 26.28124117073946 28.54861611368351 22.310091547885186 36-37 23.022055015857564 25.677030201603312 28.44785339476334 22.853061387775785 38-39 22.435897435897438 26.19178984893642 28.408431734686395 22.96388098047975 40-41 22.79123043012742 25.986668289097103 28.521243559590147 22.70085772118533 42-43 22.627171663966326 25.80657438831141 28.40265322496405 23.163600722758215 44-45 22.510076984858337 26.098230912347386 28.542655045548887 22.849037057245386 46-47 23.284699395256734 26.463018649028875 27.452227282122404 22.800054673591987 48-49 22.853109793161998 26.602597226528335 27.3399986442682 23.204294336041464 50-51 23.09748123651218 26.659821355149433 27.46903496665133 22.77366244168706 52-53 23.363931653565007 26.23865689654023 27.520735959888253 22.876675490006512 54-55 22.763244455408795 26.652625999293246 27.581188061598915 23.002941483699047 56-57 22.79357917956183 26.74272268123149 27.492429588225164 22.971268550981513 58-59 22.652943805890807 26.111356975792194 27.625244440888437 23.610454777428565 60-61 22.179982292196538 26.52970113150475 27.93449057283105 23.355826003467662 62-63 22.46526226655376 26.5488906372585 27.99860204514794 22.9872450510398 64-65 22.159945815358835 26.58625648137653 27.72967909268507 23.52411861057956 66-67 22.18861611359661 26.587117701575536 27.73367959941593 23.49058658541193 68-69 22.697597251207373 26.137005131761132 28.017715577306458 23.14768203972503 70-71 22.50895947815554 26.214544080632525 27.475871916967172 23.800624524244764 72-73 22.487617171189328 26.44845551141104 27.33170701486662 23.732220302533015 74-75 22.287545311294988 26.723690556359358 27.34115765774776 23.647606474597893 76-77 22.583990569908774 26.289379673686174 27.56040284025102 23.566226916154033 78-79 22.687873797347663 26.37264609072705 27.411416557208355 23.52806355471693 80-81 22.783152388765554 26.462181108292537 27.08067732044427 23.67398918249764 82-83 22.542133114638965 26.35506052988934 27.412361121297586 23.690445234174106 84-85 22.481381664283493 26.483167446534182 27.35655609614887 23.67889479303345 86-87 22.718766599324802 26.58692323249834 26.847484014641854 23.846826153535 88-89 22.54435561837833 26.61498234220779 26.88601667322305 23.95464536619083 90-91 22.203034606605726 26.585978668409112 27.001753555450357 24.20923316953481 92-93 22.32477224892931 26.05574483879069 27.347075073953814 24.27240783832619 94-95 22.176725718591022 25.760096278861987 27.6162758393841 24.44690216316289 96-97 22.371735070739554 25.49318774444082 27.489858431989404 24.64521875283022 98-99 22.182396134616127 25.79538657589208 27.142697995493315 24.87951929399848 100-101 21.94173592084771 25.622759784439243 27.506999501603257 24.92850479310979 102-103 21.6598607100957 25.44047952754002 27.517715443321556 25.38194431904272 104-105 21.61949294581993 25.67767107459737 27.218664163353935 25.484171816228766 106-107 21.896501861486307 25.64561667967295 26.860743155450567 25.59713830339018 108-109 21.779638532627494 25.427872521006417 27.108886107634543 25.683602838731545 110-111 21.893180093148754 25.54614597305554 27.26493052738731 25.295743406408395 112-113 21.759422860228963 25.776459462643047 27.32485003656019 25.1392676405678 114-115 21.63304574134946 25.951426069524363 27.00236474397868 25.413163445147497 116-117 21.422269042967663 25.88166723340512 27.217086386497847 25.478977337129372 118-119 21.253914384710953 26.103778700857667 26.859957816879028 25.782349097552355 120-121 21.1128076307825 26.31343682473748 27.03136468523911 25.54239085924091 122-123 21.007489607645017 26.293720747843953 26.886938567659417 25.811851076851617 124-125 20.900186745980463 26.203972616516214 27.03643963593146 25.859401001571868 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 63.5 2 134.0 3 211.5 4 149.5 5 111.5 6 265.0 7 704.0 8 1254.5 9 1467.5 10 1133.5 11 753.5 12 685.0 13 708.0 14 789.5 15 945.5 16 1080.5 17 1188.5 18 1335.5 19 1517.5 20 1796.5 21 2077.0 22 2422.5 23 2968.5 24 3677.5 25 4926.0 26 7044.0 27 9866.5 28 12738.5 29 16045.0 30 19699.5 31 25048.5 32 33527.0 33 36459.5 34 34568.5 35 35519.5 36 38863.5 37 43231.5 38 46495.5 39 49063.0 40 52134.0 41 55660.5 42 57381.0 43 58588.5 44 60242.5 45 63315.5 46 65514.0 47 67181.0 48 68056.0 49 67289.0 50 67051.5 51 65145.0 52 63923.0 53 65521.0 54 68141.0 55 65389.0 56 59364.0 57 53494.0 58 48857.0 59 43538.0 60 34159.5 61 27486.5 62 23495.5 63 16697.0 64 10983.0 65 8345.5 66 6002.0 67 4713.5 68 3708.0 69 2703.5 70 1868.5 71 1026.0 72 753.5 73 557.0 74 333.5 75 247.5 76 151.5 77 80.5 78 58.5 79 24.0 80 8.5 81 4.5 82 2.0 83 1.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026392231904930178 2 0.0 3 0.0 4 0.0 5 0.05133983637927471 6 0.013890648371015885 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.0559426416234945E-4 22-23 0.0 24-25 0.0 26-27 5.278446380986037E-4 28-29 2.2225037393625415E-4 30-31 1.3890648371015884E-4 32-33 1.3890648371015884E-4 34-35 1.9446907719422235E-4 36-37 0.0 38-39 0.0 40-41 1.666877804521906E-4 42-43 0.0 44-45 1.3890648371015884E-4 46-47 0.0 48-49 3.333755609043812E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.1112518696812707E-4 58-59 8.33438902260953E-5 60-61 2.778129674203177E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 1.1112518696812707E-4 72-73 8.33438902260953E-5 74-75 0.0 76-77 8.33438902260953E-5 78-79 0.0 80-81 1.9446907719422235E-4 82-83 0.0 84-85 2.778129674203177E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 5.556259348406354E-5 98-99 1.3890648371015884E-4 100-101 5.556259348406354E-5 102-103 2.778129674203177E-5 104-105 0.0013057209468754932 106-107 2.778129674203177E-5 108-109 0.0013612835403595566 110-111 0.0038893815438844474 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.000633413565718E-4 122-123 6.389698250667307E-4 124-125 5.556259348406354E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1799772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.976187108351 #Duplication Level Percentage of deduplicated Percentage of total 1 78.0640494687653 46.03919987905471 2 11.872082911572306 14.003403663174897 3 4.2381374083051835 7.4984755434932495 4 1.9676626682464087 4.641809667544695 5 1.0705860396657227 3.1569541295457073 6 0.6671455749555081 2.360742135425067 7 0.427429870433017 1.7645728810049086 8 0.30145318049751274 1.4222847341943052 9 0.21212345974861746 1.1259209566984703 >10 1.086048609503429 11.257970540898436 >50 0.06651051755346649 2.677168888547779 >100 0.02509714758653571 2.541390198428795 >500 0.0010457144793670635 0.4135741272571097 >1k 6.274286876202381E-4 1.096532654731794 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3616 0.20091433803837377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3300 0.18335655849740967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.10968055953754142 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.556259348406354E-5 2 0.0 0.0 0.0 0.0 5.556259348406354E-5 3 0.0 0.0 0.0 0.0 5.556259348406354E-5 4 0.0 0.0 0.0 0.0 5.556259348406354E-5 5 0.0 0.0 0.0 0.0 5.556259348406354E-5 6 0.0 0.0 0.0 0.0 5.556259348406354E-5 7 0.0 0.0 0.0 0.0 5.556259348406354E-5 8 0.0 0.0 0.0 0.0 5.556259348406354E-5 9 0.0 0.0 0.0 0.0 5.556259348406354E-5 10-11 0.0 0.0 0.0 0.0 5.556259348406354E-5 12-13 0.0 0.0 0.0 0.0 1.3890648371015884E-4 14-15 0.0 0.0 0.0 0.0 1.666877804521906E-4 16-17 0.0 0.0 0.0 0.0 1.666877804521906E-4 18-19 0.0 0.0 0.0 0.0 1.666877804521906E-4 20-21 0.0 0.0 0.0 0.0 1.9446907719422238E-4 22-23 0.0 0.0 0.0 1.666877804521906E-4 2.2225037393625415E-4 24-25 0.0 0.0 0.0 5.834072315826671E-4 2.2225037393625415E-4 26-27 0.0 0.0 0.0 0.0012779396501334612 2.2225037393625415E-4 28-29 0.0 0.0 0.0 0.004361663588498987 2.2225037393625415E-4 30-31 0.0 0.0 0.0 0.014557399492824648 2.2225037393625415E-4 32-33 0.0 0.0 0.0 0.03964391045087933 2.2225037393625415E-4 34-35 0.0 0.0 0.0 0.07642634733732939 2.2225037393625415E-4 36-37 0.0 0.0 0.0 0.13779523184047757 2.2225037393625415E-4 38-39 0.0 0.0 0.0 0.23283504799496826 2.2225037393625415E-4 40-41 0.0 0.0 0.0 0.34182107511395887 2.2225037393625415E-4 42-43 0.0 0.0 0.0 0.4509182274199176 2.2225037393625415E-4 44-45 0.0 0.0 0.0 0.5846851712328006 2.2225037393625415E-4 46-47 0.0 0.0 0.0 0.7432608130363179 2.2225037393625415E-4 48-49 0.0 0.0 0.0 0.9098652495982824 2.778129674203177E-4 50-51 0.0 0.0 0.0 1.1019451352726901 2.778129674203177E-4 52-53 0.0 0.0 0.0 1.2769673047474903 3.333755609043812E-4 54-55 0.0 0.0 0.0 1.4675470003978281 3.333755609043812E-4 56-57 0.0 0.0 0.0 1.6866303065054908 3.333755609043812E-4 58-59 0.0 0.0 0.0 1.9320502819245993 3.333755609043812E-4 60-61 0.0 0.0 0.0 2.1997786386275595 3.333755609043812E-4 62-63 0.0 0.0 0.0 2.465840117525998 4.445007478725083E-4 64-65 0.0 0.0 0.0 2.7394581091382686 4.445007478725083E-4 66-67 5.556259348406354E-5 0.0 0.0 3.0583873957367933 4.445007478725083E-4 68-69 5.556259348406354E-5 0.0 0.0 3.419294221712528 4.445007478725083E-4 70-71 5.556259348406354E-5 0.0 0.0 3.7944528529169252 4.7228204461454006E-4 72-73 5.556259348406354E-5 0.0 0.0 4.201587756671401 5.278446380986037E-4 74-75 5.556259348406354E-5 0.0 0.0 4.6041943090569255 5.556259348406354E-4 76-77 5.556259348406354E-5 0.0 0.0 5.041249669402569 6.111885283246989E-4 78-79 5.556259348406354E-5 0.0 0.0 5.5236163247344665 6.667511218087624E-4 80-81 5.556259348406354E-5 0.0 0.0 6.035764530173822 6.945324185507942E-4 82-83 5.556259348406354E-5 0.0 0.0 6.581333635593841 7.22313715292826E-4 84-85 5.556259348406354E-5 0.0 0.0 7.123930142262465 7.22313715292826E-4 86-87 5.556259348406354E-5 0.0 0.0 7.675083288327633 7.778763087768895E-4 88-89 5.556259348406354E-5 0.0 0.0 8.275742705187103 8.334389022609531E-4 90-91 5.556259348406354E-5 0.0 0.0 8.920935540724049 8.334389022609531E-4 92-93 5.556259348406354E-5 0.0 0.0 9.582435997448567 8.334389022609531E-4 94-95 5.556259348406354E-5 0.0 0.0 10.245797801054799 8.334389022609531E-4 96-97 5.556259348406354E-5 0.0 0.0 10.921022218369883 8.334389022609531E-4 98-99 5.556259348406354E-5 0.0 0.0 11.61586023118484 8.334389022609531E-4 100-101 5.556259348406354E-5 0.0 0.0 12.375512009298955 8.334389022609531E-4 102-103 5.556259348406354E-5 0.0 0.0 13.181864147236428 8.334389022609531E-4 104-105 5.556259348406354E-5 0.0 0.0 13.98618825051173 8.334389022609531E-4 106-107 5.556259348406354E-5 0.0 0.0 14.781761245313294 8.334389022609531E-4 108-109 5.556259348406354E-5 0.0 0.0 15.604170972767662 8.334389022609531E-4 110-111 5.556259348406354E-5 0.0 0.0 16.479087351064468 8.612201990029849E-4 112-113 5.556259348406354E-5 0.0 0.0 17.374117388202507 8.890014957450166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2775 0.0 80.05261 1 GTATCAA 5420 0.0 59.886215 1 ATCAACG 7375 0.0 43.5195 3 TATCAAC 7410 0.0 43.474663 2 TCAACGC 7380 0.0 43.40933 4 CAACGCA 7435 0.0 43.08821 5 AACGCAG 7870 0.0 40.83294 6 ACGCAGA 9305 0.0 34.46414 7 CGCAGAG 9420 0.0 34.0434 8 GTACATG 8660 0.0 33.49197 1 TACATGG 8815 0.0 32.69492 2 TGGTATC 985 0.0 30.83125 2 ACATGGG 9045 0.0 30.67853 3 CATGGGA 5965 0.0 29.149397 4 GCAGAGT 11025 0.0 28.547768 9 AGGTAAG 1165 0.0 27.089882 2 TAAGGTG 1455 0.0 27.010841 5 GAGTACT 5950 0.0 25.898693 12-13 CAGAGTA 10205 0.0 25.623648 10-11 AGAGTAC 9965 0.0 25.375038 10-11 >>END_MODULE