FastQCFastQC Report
Thu 26 May 2016
SRR1364366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118942
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.059.5383871
GTATCAA37550.048.3600731
ATCAACG41200.041.756673
TCAACGC41350.041.4612354
CAACGCA42100.040.5812155
GTACATG105150.037.9369661
TATCAAC47100.037.5371062
AACGCAG45550.037.368936
TACATGG112750.034.7931822
ACATGGG110300.034.1628043
ACGCAGA55000.030.7202437
CATGGGA69750.030.2976824
CGCAGAG56150.029.8791588
TAAGGTG12550.027.51125
CATGGGG44150.027.5058294
GTAAGGT11100.027.3509544
ATGGGAG29350.023.7302885
GCAGAGT70000.023.627399
CCCTATA5300.023.5867822
GTATAGG7850.023.5119741
Produced by FastQC (version 0.11.5)