FastQCFastQC Report
Thu 26 May 2016
SRR1364366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118942
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.1837709573928876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.16451606509286237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.074.552471
GTATCAA66250.056.010581
ATCAACG80000.045.9251143
TCAACGC82000.044.732374
CAACGCA82950.044.2200625
TATCAAC85500.043.1101532
AACGCAG85950.042.6484036
ACGCAGA101650.036.0553367
CGCAGAG103200.035.4561548
GTACATG105650.034.84071
ACATGGG110100.031.909483
TACATGG114100.031.8867762
TACGGTA750.00389634731.7580452
GCAGAGT118650.030.3879289
TAAGGTG11200.030.3048325
AGGTAAG11350.029.904332
GTGGTAT17650.029.021621
GTAAGGT11550.028.8709494
GAGTACT63700.027.64703212-13
CATGGGG43000.027.280534
Produced by FastQC (version 0.11.5)