FastQCFastQC Report
Thu 26 May 2016
SRR1364365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637947
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23100.054.13471
ATCAACG25900.045.7294733
CAACGCA26900.044.473365
TCAACGC27150.043.844624
AACGCAG27800.042.812946
GGTATCA15800.042.588251
GTACATG66250.042.155611
TATCAAC29750.040.6117742
TACATGG70150.039.367082
ACATGGG71300.037.897383
ACGCAGA32900.035.986617
CGCAGAG34100.034.19688
CATGGGG41200.032.215424
CATGGGA37450.029.5608774
GCAGAGT42900.025.6565489
ATGGGAG20200.024.7505055
ATGGGGA23600.024.7155485
AGTACAT48300.023.289362
GAGTACA49750.022.861711
GTATAGG5200.021.7579861
Produced by FastQC (version 0.11.5)